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Protein

Syntaxin VAM3

Gene

VAM3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Required for vacuolar assembly. Provides the t-SNARE function in a late step of the vacuolar assembly. Required for homotypic vacuole membrane fusion, autophagy and fusion of biosynthetic transport vesicles with the vacuole. Required for the delivery of alpha-factor receptor-ligand complexes to the vacuole.

GO - Molecular functioni

  • SNAP receptor activity Source: SGD
  • SNARE binding Source: GO_Central

GO - Biological processi

  • autophagosome maturation Source: SGD
  • Golgi to vacuole transport Source: GO_Central
  • intracellular protein transport Source: GO_Central
  • piecemeal microautophagy of nucleus Source: SGD
  • vacuole fusion, non-autophagic Source: SGD
  • vesicle docking Source: SGD
  • vesicle fusion Source: SGD
  • vesicle fusion with vacuole Source: SGD
Complete GO annotation...

Keywords - Biological processi

Autophagy

Enzyme and pathway databases

BioCyciYEAST:G3O-33637-MONOMER.

Protein family/group databases

TCDBi1.F.1.1.2. the synaptosomal vesicle fusion pore (svf-pore) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Syntaxin VAM3
Alternative name(s):
Vacuolar morphogenesis protein 3
Gene namesi
Name:VAM3
Synonyms:PTH1
Ordered Locus Names:YOR106W
ORF Names:YOR3220W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XV

Organism-specific databases

EuPathDBiFungiDB:YOR106W.
SGDiS000005632. VAM3.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 261CytoplasmicSequence analysisAdd BLAST261
Transmembranei262 – 282Helical; Anchor for type IV membrane proteinSequence analysisAdd BLAST21
Topological domaini283VacuolarSequence analysis1

GO - Cellular componenti

  • endomembrane system Source: GO_Central
  • fungal-type vacuole membrane Source: SGD
  • integral component of membrane Source: GO_Central
  • SNARE complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Vacuole

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002102801 – 283Syntaxin VAM3Add BLAST283

Proteomic databases

MaxQBiQ12241.
PRIDEiQ12241.

PTM databases

iPTMnetiQ12241.
SwissPalmiQ12241.

Interactioni

Subunit structurei

Associates with VAM7.

Binary interactionsi

WithEntry#Exp.IntActNotes
NYV1Q1225510EBI-20227,EBI-35465
VAM7P329125EBI-20227,EBI-20232

GO - Molecular functioni

  • SNAP receptor activity Source: SGD
  • SNARE binding Source: GO_Central

Protein-protein interaction databases

BioGridi34502. 221 interactors.
DIPiDIP-1726N.
IntActiQ12241. 6 interactors.
MINTiMINT-384960.

Structurei

Secondary structure

1283
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi25 – 50Combined sources26
Beta strandi53 – 55Combined sources3
Helixi58 – 66Combined sources9
Helixi68 – 81Combined sources14
Helixi84 – 87Combined sources4
Helixi89 – 115Combined sources27

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1HS7NMR-A23-119[»]
ProteinModelPortaliQ12241.
SMRiQ12241.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ12241.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini190 – 252t-SNARE coiled-coil homologyPROSITE-ProRule annotationAdd BLAST63

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili28 – 48Sequence analysisAdd BLAST21
Coiled coili84 – 111Sequence analysisAdd BLAST28
Coiled coili169 – 189Sequence analysisAdd BLAST21

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi174 – 179Poly-Gln6
Compositional biasi268 – 271Poly-Ile4

Sequence similaritiesi

Belongs to the syntaxin family.Curated
Contains 1 t-SNARE coiled-coil homology domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil, Transmembrane, Transmembrane helix

Phylogenomic databases

HOGENOMiHOG000141965.
InParanoidiQ12241.
KOiK08488.
OMAiQASHELT.
OrthoDBiEOG092C42X0.

Family and domain databases

InterProiIPR006012. Syntaxin/epimorphin_CS.
IPR010989. t-SNARE.
IPR000727. T_SNARE_dom.
[Graphical view]
PfamiPF05739. SNARE. 1 hit.
[Graphical view]
SMARTiSM00397. t_SNARE. 1 hit.
[Graphical view]
SUPFAMiSSF47661. SSF47661. 1 hit.
PROSITEiPS00914. SYNTAXIN. 1 hit.
PS50192. T_SNARE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q12241-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSFFDIEAQS SKGNSQQEPQ FSTNQKTKEL SNLIETFAEQ SRVLEKECTK
60 70 80 90 100
IGSKRDSKEL RYKIETELIP NCTSVRDKIE SNILIHQNGK LSADFKNLKT
110 120 130 140 150
KYQSLQQSYN QRKSLFPLKT PISPGTSKER KDIHPRTEAV RQDPESSYIS
160 170 180 190 200
IKVNEQSPLL HNEGQHQLQL QEEQEQQQQG LSQEELDFQT IIHQERSQQI
210 220 230 240 250
GRIHTAVQEV NAIFHQLGSL VKEQGEQVTT IDENISHLHD NMQNANKQLT
260 270 280
RADQHQRDRN KCGKVTLIII IVVCMVVLLA VLS
Length:283
Mass (Da):32,498
Last modified:November 1, 1997 - v1
Checksum:i6415D9D852C6C64D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U57827 Genomic DNA. Translation: AAC49737.1.
X94335 Genomic DNA. Translation: CAA64026.1.
Z75014 Genomic DNA. Translation: CAA99304.1.
BK006948 Genomic DNA. Translation: DAA10881.1.
PIRiS61664.
RefSeqiNP_014749.1. NM_001183525.1.

Genome annotation databases

EnsemblFungiiYOR106W; YOR106W; YOR106W.
GeneIDi854273.
KEGGisce:YOR106W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U57827 Genomic DNA. Translation: AAC49737.1.
X94335 Genomic DNA. Translation: CAA64026.1.
Z75014 Genomic DNA. Translation: CAA99304.1.
BK006948 Genomic DNA. Translation: DAA10881.1.
PIRiS61664.
RefSeqiNP_014749.1. NM_001183525.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1HS7NMR-A23-119[»]
ProteinModelPortaliQ12241.
SMRiQ12241.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34502. 221 interactors.
DIPiDIP-1726N.
IntActiQ12241. 6 interactors.
MINTiMINT-384960.

Protein family/group databases

TCDBi1.F.1.1.2. the synaptosomal vesicle fusion pore (svf-pore) family.

PTM databases

iPTMnetiQ12241.
SwissPalmiQ12241.

Proteomic databases

MaxQBiQ12241.
PRIDEiQ12241.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYOR106W; YOR106W; YOR106W.
GeneIDi854273.
KEGGisce:YOR106W.

Organism-specific databases

EuPathDBiFungiDB:YOR106W.
SGDiS000005632. VAM3.

Phylogenomic databases

HOGENOMiHOG000141965.
InParanoidiQ12241.
KOiK08488.
OMAiQASHELT.
OrthoDBiEOG092C42X0.

Enzyme and pathway databases

BioCyciYEAST:G3O-33637-MONOMER.

Miscellaneous databases

EvolutionaryTraceiQ12241.
PROiQ12241.

Family and domain databases

InterProiIPR006012. Syntaxin/epimorphin_CS.
IPR010989. t-SNARE.
IPR000727. T_SNARE_dom.
[Graphical view]
PfamiPF05739. SNARE. 1 hit.
[Graphical view]
SMARTiSM00397. t_SNARE. 1 hit.
[Graphical view]
SUPFAMiSSF47661. SSF47661. 1 hit.
PROSITEiPS00914. SYNTAXIN. 1 hit.
PS50192. T_SNARE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiVAM3_YEAST
AccessioniPrimary (citable) accession number: Q12241
Secondary accession number(s): D6W2G5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: November 2, 2016
This is version 144 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 414 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XV
    Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.