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Protein

Serine/threonine-protein kinase PKH2

Gene

PKH2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Serine/threonine-protein kinase which is part sphingolipid-mediated signaling pathway that is required for the internalization step of endocytosis by regulating eisosome assembly and organization, and modulating the organization of the plasma membrane. Phosphorylates and activates PKC1. Activates YPK1 and YPK2, 2 components of signaling cascade required for maintenance of cell wall integrity. Required for stress-induced P-body assembly and regulates global mRNA decay at the deadenylation step.7 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Enzyme regulationi

Sphingoid base activates kinase activity.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei208ATPBy similarity1
Binding sitei264ATPBy similarity1
Active sitei303Proton acceptorPROSITE-ProRule annotation1
Binding sitei307ATP; via carbonyl oxygenBy similarity1
Binding sitei321ATPBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi189 – 191ATPBy similarity3
Nucleotide bindingi258 – 260ATPBy similarity3

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • protein kinase activity Source: SGD
  • protein serine/threonine kinase activity Source: SGD

GO - Biological processi

  • endocytosis Source: UniProtKB-KW
  • late endosome to vacuole transport via multivesicular body sorting pathway Source: SGD
  • MAPK cascade involved in cell wall organization or biogenesis Source: SGD
  • peptidyl-serine phosphorylation Source: SGD
  • positive regulation of cytoplasmic mRNA processing body assembly Source: SGD
  • positive regulation of protein serine/threonine kinase activity Source: SGD
  • protein phosphorylation Source: SGD
  • regulation of nuclear-transcribed mRNA poly(A) tail shortening Source: SGD

Keywordsi

Molecular functionKinase, Serine/threonine-protein kinase, Transferase
Biological processEndocytosis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-33498-MONOMER.
ReactomeiR-SCE-114604. GPVI-mediated activation cascade.
R-SCE-1257604. PIP3 activates AKT signaling.
R-SCE-165158. Activation of AKT2.
R-SCE-202424. Downstream TCR signaling.
R-SCE-2730905. Role of LAT2/NTAL/LAB on calcium mobilization.
R-SCE-389357. CD28 dependent PI3K/Akt signaling.
R-SCE-389513. CTLA4 inhibitory signaling.
R-SCE-392451. G beta:gamma signalling through PI3Kgamma.
R-SCE-444257. RSK activation.
R-SCE-5218920. VEGFR2 mediated vascular permeability.
R-SCE-5218921. VEGFR2 mediated cell proliferation.
R-SCE-5625740. RHO GTPases activate PKNs.
R-SCE-6804757. Regulation of TP53 Degradation.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein kinase PKH2 (EC:2.7.11.1)
Alternative name(s):
PKB-activating kinase homolog 2
Gene namesi
Name:PKH2
Ordered Locus Names:YOL100W
ORF Names:HRC1081
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XV

Organism-specific databases

EuPathDBiFungiDB:YOL100W.
SGDiS000005460. PKH2.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Disruption phenotypei

Long-term depletion of PKH2 in a PKH1 null mutant (Pkh depletion) induces programmed cell death. This is mediated by the lack of Pkh-dependent activation of the PKC1 downstream signaling cascade and results in accumulation of reactive oxigen species (ROS) and DNA fragmentation.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000861581 – 1081Serine/threonine-protein kinase PKH2Add BLAST1081

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei138PhosphoserineCombined sources1
Modified residuei619PhosphoserineCombined sources1
Modified residuei1009PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ12236.
PRIDEiQ12236.

PTM databases

iPTMnetiQ12236.

Interactioni

Protein-protein interaction databases

BioGridi34303. 87 interactors.
DIPiDIP-6295N.
IntActiQ12236. 10 interactors.
MINTiMINT-678158.
STRINGi4932.YOL100W.

Structurei

3D structure databases

ProteinModelPortaliQ12236.
SMRiQ12236.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini179 – 443Protein kinasePROSITE-ProRule annotationAdd BLAST265

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni210 – 255PIF-pocketBy similarityAdd BLAST46

Domaini

The PIF-pocket is a small lobe in the catalytic domain required by the enzyme for the binding to the hydrophobic motif of its substrates. It is an allosteric regulatory site that can accommodate small compounds acting as allosteric inhibitors.By similarity

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00550000074819.
InParanoidiQ12236.
KOiK06276.
OMAiFQMIAGK.
OrthoDBiEOG092C0HH3.

Family and domain databases

InterProiView protein in InterPro
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
PfamiView protein in Pfam
PF00069. Pkinase. 1 hit.
SMARTiView protein in SMART
SM00220. S_TKc. 1 hit.
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiView protein in PROSITE
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.

Sequencei

Sequence statusi: Complete.

Q12236-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MYFDKDNSMS PRPLLPSDEQ KLNINLLTKK EKFSHLDPHY DAKATPQRST
60 70 80 90 100
SNRNVGDLLL EKRTAKPMIQ KALTNTDNFI EMYHNQQRKN LDDDTIKEVM
110 120 130 140 150
INDENGKTVA STNDGRYDND YDNNDINDQK TLDNIAGSPH MEKNRNKVKI
160 170 180 190 200
EHDSSSQKPI AKESSKAQKN IIKKGIKDFK FGSVIGDGAY STVMLATSID
210 220 230 240 250
TKKRYAAKVL NKEYLIRQKK VKYVSIEKTA LQKLNNSPSV VRLFSTFQDE
260 270 280 290 300
SSLYFLLEYA PNGDFLSLMK KYGSLDETCA RYYAAQIIDA IDYLHSNGII
310 320 330 340 350
HRDIKPENIL LDGEMKIKLT DFGTAKLLNP TNNSVSKPEY DLSTRSKSFV
360 370 380 390 400
GTAEYVSPEL LNDSFTDYRC DIWAFGCILF QMIAGKPPFK ATNEYLTFQK
410 420 430 440 450
VMKVQYAFTP GFPLIIRDLV KKILVKNLDR RLTISQIKEH HFFKDLNFKD
460 470 480 490 500
GSVWSKTPPE IKPYKINAKS MQAMPSGSDR KLVKKSVNTL GKSHLVTQRS
510 520 530 540 550
ASSPSVEETT HSTLYNNNTH ASTESEISIK KRPTDERTAQ ILENARKGIN
560 570 580 590 600
NRKNQPGKRT PSGAASAALA ASAALTKKTM QSYPTSSSKS SRSSSPATTS
610 620 630 640 650
RPGTYKRTSS TESKPFAKSP PLSASVLSSK VPMPPYTPPM SPPMTPYDTY
660 670 680 690 700
QMTPPYTTKQ QDYSDTAIAA PKPCISKQNV KNSTDSPLMN KQDIQWSFYL
710 720 730 740 750
KNINEHVLRT EKLDFVTTNY DILEKKMLKL NGSLLDPQLF GKPRHTFLSQ
760 770 780 790 800
VARSGGEVTG FRNDPTMTAY SKTEDTYYSK NIIDLQLLED DYRIEGGDLS
810 820 830 840 850
ELLTNRSGEG YKCNQNSSPM KDDDKSESNN KGSSVFSGKI KKLFHPTSAA
860 870 880 890 900
ETLSSSDEKT KYYKRTIVMT SFGRFLVFAK RRQPNPVTNL KYELEYDINL
910 920 930 940 950
RQQGTKIKEL IIPLEMGTNH IVVIQTPYKS FLLSTDKKTT SKLFTVLKKI
960 970 980 990 1000
LNSNTNKIEK ELLQRNQKVI ERRTSSSGRA IPKDLPTSKS PSPKPRTHSQ
1010 1020 1030 1040 1050
SPSISKHNSF SESINSAKSN RSSRIFETFI NAKEQNSKKH AAPVPLTSKL
1060 1070 1080
VNGLPKRQVT VGLGLNTGTN FKNSSAKSKR S
Length:1,081
Mass (Da):121,660
Last modified:November 1, 1996 - v1
Checksum:iBE0DD9D49AC2EBC3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z48149 Genomic DNA. Translation: CAA88162.1.
Z74842 Genomic DNA. Translation: CAA99113.1.
BK006948 Genomic DNA. Translation: DAA10684.1.
PIRiS51899.
RefSeqiNP_014541.1. NM_001183354.1.

Genome annotation databases

EnsemblFungiiYOL100W; YOL100W; YOL100W.
GeneIDi854053.
KEGGisce:YOL100W.

Similar proteinsi

Entry informationi

Entry nameiPKH2_YEAST
AccessioniPrimary (citable) accession number: Q12236
Secondary accession number(s): D6W1W8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: September 27, 2017
This is version 159 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XV
    Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names