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Protein

High affinity cysteine transporter

Gene

YCT1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

High affinity cysteine-specific transporter. Major contributor to cysteine transport when cysteine, at low concentrations, is provided as the sole sulfur source.1 Publication

Kineticsi

  1. KM=55.4 µM for cysteine1 Publication
  1. Vmax=22.4 nmol/min/mg enzyme for cysteine transport1 Publication

GO - Molecular functioni

  • cysteine transmembrane transporter activity Source: SGD
  • ion transmembrane transporter activity Source: SGD

GO - Biological processi

  • cysteine transport Source: SGD
Complete GO annotation...

Keywords - Biological processi

Amino-acid transport, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-32154-MONOMER.

Protein family/group databases

TCDBi2.A.1.14.20. the major facilitator superfamily (mfs).

Names & Taxonomyi

Protein namesi
Recommended name:
High affinity cysteine transporter
Gene namesi
Name:YCT1
Ordered Locus Names:YLL055W
ORF Names:L0578
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XII

Organism-specific databases

EuPathDBiFungiDB:YLL055W.
SGDiS000003978. YCT1.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 54CytoplasmicSequence analysisAdd BLAST54
Transmembranei55 – 75HelicalSequence analysisAdd BLAST21
Topological domaini76 – 97LumenalSequence analysisAdd BLAST22
Transmembranei98 – 118HelicalSequence analysisAdd BLAST21
Topological domaini119 – 120CytoplasmicSequence analysis2
Transmembranei121 – 141HelicalSequence analysisAdd BLAST21
Topological domaini142 – 154LumenalSequence analysisAdd BLAST13
Transmembranei155 – 175HelicalSequence analysisAdd BLAST21
Topological domaini176 – 186CytoplasmicSequence analysisAdd BLAST11
Transmembranei187 – 207HelicalSequence analysisAdd BLAST21
Topological domaini208 – 218LumenalSequence analysisAdd BLAST11
Transmembranei219 – 239HelicalSequence analysisAdd BLAST21
Topological domaini240 – 285CytoplasmicSequence analysisAdd BLAST46
Transmembranei286 – 306HelicalSequence analysisAdd BLAST21
Topological domaini307 – 324LumenalSequence analysisAdd BLAST18
Transmembranei325 – 345HelicalSequence analysisAdd BLAST21
Topological domaini346 – 352CytoplasmicSequence analysis7
Transmembranei353 – 373HelicalSequence analysisAdd BLAST21
Topological domaini374 – 378LumenalSequence analysis5
Transmembranei379 – 399HelicalSequence analysisAdd BLAST21
Topological domaini400 – 413CytoplasmicSequence analysisAdd BLAST14
Transmembranei414 – 436HelicalSequence analysisAdd BLAST23
Topological domaini437 – 447LumenalSequence analysisAdd BLAST11
Transmembranei448 – 468HelicalSequence analysisAdd BLAST21
Topological domaini469 – 531CytoplasmicSequence analysisAdd BLAST63

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002471511 – 531High affinity cysteine transporterAdd BLAST531

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi146N-linked (GlcNAc...)Sequence analysis1
Modified residuei500PhosphoserineCombined sources1
Modified residuei501PhosphoserineCombined sources1

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

PRIDEiQ12235.

PTM databases

iPTMnetiQ12235.

Expressioni

Inductioni

Induced under nitrogen starvation conditions, co-regulated by GCN4 and GLN3. Regulated by the MET4-based sulfur regulatory network. Expression is maximum in nonrepressing sulfur conditions and considerably repressed in the presence of organic sulfur. Induced by chromate.4 Publications

Interactioni

Protein-protein interaction databases

BioGridi31260. 18 interactors.
MINTiMINT-2494190.

Structurei

3D structure databases

ProteinModelPortaliQ12235.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili469 – 498Sequence analysisAdd BLAST30

Sequence similaritiesi

Keywords - Domaini

Coiled coil, Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00620000088258.
InParanoidiQ12235.
OMAiLANICMA.
OrthoDBiEOG092C1ZH9.

Family and domain databases

CDDicd06174. MFS. 1 hit.
InterProiIPR011701. MFS.
IPR020846. MFS_dom.
[Graphical view]
PfamiPF07690. MFS_1. 1 hit.
[Graphical view]
SUPFAMiSSF103473. SSF103473. 1 hit.

Sequencei

Sequence statusi: Complete.

Q12235-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSKVDVKIGA DSISSSDEIL VPSRLADVTL AFMEENDAAV PEITPEQEKK
60 70 80 90 100
LKRKLFLTIF TFVSAINLLL YMDKATLSYD SILGFFEDTG LTQNTYNTVN
110 120 130 140 150
TLFYVGFAIG QFPGQYLAQK LPLGKFLGGL LATWTILIFL SCTAYNFSGV
160 170 180 190 200
VALRFFLGLT ESVVIPILIT TMGMFFDASE RAAAQPFFFA ACMGSPIPTG
210 220 230 240 250
FIAYGVLHIT NPSISLWKIF TIIIGGLTFI MTVVVILWFP NNPADVKFFS
260 270 280 290 300
IQERVWIIRR VQASTGSSIE QKVFKKSQFR EAMKDYITWL FGLFFLLQQL
310 320 330 340 350
ANNLPYQQNL LFEGMGGVDA LGSTLVSVAG AGFAVVCAFI ATLMLAKWKN
360 370 380 390 400
ISALTAIFWT LPALVGSIAA AALPWDNKIG ILANICMAGQ IFGIPFIIAL
410 420 430 440 450
SWASSSASGY TKKLTRSSVS LFAMGIANII SPQIWREKDS PRFLPAWIVQ
460 470 480 490 500
IVLSFSLAPA ILLLIHFILK RRNNQRLKNY DENLQNYLDR IQLIESENPS
510 520 530
SIEEGKVVTH ENNLAVFDLT DLENETFIYP L
Length:531
Mass (Da):58,905
Last modified:November 1, 1996 - v1
Checksum:i7C008873BBB54A0D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z47973 Genomic DNA. Translation: CAA88002.1.
Z73160 Genomic DNA. Translation: CAA97508.1.
AY692634 Genomic DNA. Translation: AAT92653.1.
BK006945 Genomic DNA. Translation: DAA09269.1.
PIRiS50965.
RefSeqiNP_013045.1. NM_001181875.1.

Genome annotation databases

EnsemblFungiiYLL055W; YLL055W; YLL055W.
GeneIDi850671.
KEGGisce:YLL055W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z47973 Genomic DNA. Translation: CAA88002.1.
Z73160 Genomic DNA. Translation: CAA97508.1.
AY692634 Genomic DNA. Translation: AAT92653.1.
BK006945 Genomic DNA. Translation: DAA09269.1.
PIRiS50965.
RefSeqiNP_013045.1. NM_001181875.1.

3D structure databases

ProteinModelPortaliQ12235.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi31260. 18 interactors.
MINTiMINT-2494190.

Protein family/group databases

TCDBi2.A.1.14.20. the major facilitator superfamily (mfs).

PTM databases

iPTMnetiQ12235.

Proteomic databases

PRIDEiQ12235.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYLL055W; YLL055W; YLL055W.
GeneIDi850671.
KEGGisce:YLL055W.

Organism-specific databases

EuPathDBiFungiDB:YLL055W.
SGDiS000003978. YCT1.

Phylogenomic databases

GeneTreeiENSGT00620000088258.
InParanoidiQ12235.
OMAiLANICMA.
OrthoDBiEOG092C1ZH9.

Enzyme and pathway databases

BioCyciYEAST:G3O-32154-MONOMER.

Miscellaneous databases

PROiQ12235.

Family and domain databases

CDDicd06174. MFS. 1 hit.
InterProiIPR011701. MFS.
IPR020846. MFS_dom.
[Graphical view]
PfamiPF07690. MFS_1. 1 hit.
[Graphical view]
SUPFAMiSSF103473. SSF103473. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiYCT1_YEAST
AccessioniPrimary (citable) accession number: Q12235
Secondary accession number(s): D6VXV3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 25, 2006
Last sequence update: November 1, 1996
Last modified: November 2, 2016
This is version 121 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XII
    Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.