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Protein

Sphingolipid long chain base-responsive protein LSP1

Gene

LSP1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Together with PIL1, main component of eisosomes, structures at the cell periphery underneath the plasma membrane that mark the site of endocytosis. Negative regulator of cell wall integrity (CWI) in unstressed cells, probably by inhibiting protein kinase PKH1/PHK2 activity and regulating their downstream CWI pathways PKC1-MAP kinase pathway and protein kinase YPK1 pathway. Activity may be regulated by the transient increase of sphingolipid long chain bases (LCBs) during heat stress.1 Publication

Miscellaneous

Present with 104485 molecules/cell in log phase SD medium.1 Publication

GO - Molecular functioni

  • lipid binding Source: SGD

GO - Biological processi

  • eisosome assembly Source: GO_Central
  • endocytosis Source: SGD
  • negative regulation of protein kinase activity Source: SGD
  • response to heat Source: SGD

Enzyme and pathway databases

BioCyciYEAST:G3O-33923-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Sphingolipid long chain base-responsive protein LSP1
Gene namesi
Name:LSP1
Ordered Locus Names:YPL004C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XVI

Organism-specific databases

EuPathDBiFungiDB:YPL004C
SGDiS000005925 LSP1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000845051 – 341Sphingolipid long chain base-responsive protein LSP1Add BLAST341

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei233PhosphothreonineCombined sources1

Post-translational modificationi

Phosphorylated by PKH1 and PKH2. Phosphorylation is stimulated by sphingolipid long chain bases (LCBs).1 Publication
N-glycosylated.1 Publication

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

MaxQBiQ12230
PaxDbiQ12230
PRIDEiQ12230
TopDownProteomicsiQ12230

PTM databases

iPTMnetiQ12230

Interactioni

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi36173, 151 interactors
DIPiDIP-3993N
IntActiQ12230, 92 interactors
MINTiQ12230
STRINGi4932.YPL004C

Structurei

Secondary structure

1341
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Turni53 – 55Combined sources3
Helixi56 – 91Combined sources36
Helixi95 – 159Combined sources65
Helixi165 – 224Combined sources60
Beta strandi234 – 236Combined sources3
Helixi244 – 260Combined sources17

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3PLTX-ray2.90A/B/C36-267[»]
ProteinModelPortaliQ12230
SMRiQ12230
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ12230

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi21 – 25Poly-Pro5
Compositional biasi283 – 324Glu-richAdd BLAST42

Phylogenomic databases

GeneTreeiENSGT00390000012909
HOGENOMiHOG000214828
InParanoidiQ12230
OMAiDKYDQYR
OrthoDBiEOG092C3P1A

Family and domain databases

Gene3Di1.20.1270.60, 1 hit
InterProiView protein in InterPro
IPR027267 AH/BAR_dom_sf
IPR028245 PIL1/LSP1
PfamiView protein in Pfam
PF13805 Pil1, 1 hit

Sequencei

Sequence statusi: Complete.

Q12230-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHRTYSLRNQ RAPTAAELQA PPPPPSSTKS KFFGKASIAS SFRKNAAGNF
60 70 80 90 100
GPELARKLSQ LVKTEKGVLR AMEVVASERR EAAKQLSLWG ADNDDDVSDV
110 120 130 140 150
TDKLGVLIYE LGELQDQFID KYDQYRVTLK SIRNIEASVQ PSRDRKEKIT
160 170 180 190 200
DEIAHLKYKD PQSTKIPVLE QELVRAEAES LVAEAQLSNI TREKLKAAYS
210 220 230 240 250
YMFDSLRELS EKFALIAGYG KALLELLDDS PVTPGEARPA YDGYEASRQI
260 270 280 290 300
IMDAESALES WTLDMAAVKP TLSFHQTVDD VYEDEDGEEE EEPEIQNGDI
310 320 330 340
PGQVVEEEEV EWTTEVPVDD EAHEADHHVS QNGHTSGSEN I
Length:341
Mass (Da):38,071
Last modified:November 1, 1996 - v1
Checksum:i6FCEE022ECC9778C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U33335 Genomic DNA Translation: AAB68101.1
Z48483 Genomic DNA Translation: CAA88382.1
Z71255 Genomic DNA Translation: CAA95037.1
BK006949 Genomic DNA Translation: DAA11424.1
PIRiS52527
RefSeqiNP_015321.1, NM_001183818.1

Genome annotation databases

EnsemblFungiiYPL004C; YPL004C; YPL004C
GeneIDi856103
KEGGisce:YPL004C

Similar proteinsi

Entry informationi

Entry nameiLSP1_YEAST
AccessioniPrimary (citable) accession number: Q12230
Secondary accession number(s): D6W408
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 22, 2005
Last sequence update: November 1, 1996
Last modified: March 28, 2018
This is version 146 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome
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Main funding by: National Institutes of Health