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Protein

Sphingolipid long chain base-responsive protein LSP1

Gene

LSP1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Together with PIL1, main component of eisosomes, structures at the cell periphery underneath the plasma membrane that mark the site of endocytosis. Negative regulator of cell wall integrity (CWI) in unstressed cells, probably by inhibiting protein kinase PKH1/PHK2 activity and regulating their downstream CWI pathways PKC1-MAP kinase pathway and protein kinase YPK1 pathway. Activity may be regulated by the transient increase of sphingolipid long chain bases (LCBs) during heat stress.1 Publication

GO - Molecular functioni

  • lipid binding Source: SGD

GO - Biological processi

  • endocytosis Source: SGD
  • negative regulation of protein kinase activity Source: SGD
  • response to heat Source: SGD
Complete GO annotation...

Enzyme and pathway databases

BioCyciYEAST:G3O-33923-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Sphingolipid long chain base-responsive protein LSP1
Gene namesi
Name:LSP1
Ordered Locus Names:YPL004C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XVI

Organism-specific databases

EuPathDBiFungiDB:YPL004C.
SGDiS000005925. LSP1.

Subcellular locationi

GO - Cellular componenti

  • cell cortex Source: UniProtKB-SubCell
  • eisosome Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 341341Sphingolipid long chain base-responsive protein LSP1PRO_0000084505Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei233 – 2331PhosphothreonineCombined sources

Post-translational modificationi

Phosphorylated by PKH1 and PKH2. Phosphorylation is stimulated by sphingolipid long chain bases (LCBs).1 Publication
N-glycosylated.1 Publication

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

MaxQBiQ12230.
PRIDEiQ12230.
TopDownProteomicsiQ12230.

PTM databases

iPTMnetiQ12230.

Interactioni

Protein-protein interaction databases

BioGridi36173. 124 interactions.
DIPiDIP-3993N.
IntActiQ12230. 46 interactions.
MINTiMINT-546535.

Structurei

Secondary structure

1
341
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Turni53 – 553Combined sources
Helixi56 – 9136Combined sources
Helixi95 – 15965Combined sources
Helixi165 – 22460Combined sources
Beta strandi234 – 2363Combined sources
Helixi244 – 26017Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3PLTX-ray2.90A/B/C36-267[»]
ProteinModelPortaliQ12230.
SMRiQ12230. Positions 51-264.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ12230.

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi21 – 255Poly-Pro
Compositional biasi283 – 32442Glu-richAdd
BLAST

Phylogenomic databases

GeneTreeiENSGT00390000012909.
HOGENOMiHOG000214828.
InParanoidiQ12230.
OMAiYSMRQTR.
OrthoDBiEOG092C3P1A.

Family and domain databases

InterProiIPR028245. PIL1/LSP1.
[Graphical view]
PfamiPF13805. Pil1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q12230-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHRTYSLRNQ RAPTAAELQA PPPPPSSTKS KFFGKASIAS SFRKNAAGNF
60 70 80 90 100
GPELARKLSQ LVKTEKGVLR AMEVVASERR EAAKQLSLWG ADNDDDVSDV
110 120 130 140 150
TDKLGVLIYE LGELQDQFID KYDQYRVTLK SIRNIEASVQ PSRDRKEKIT
160 170 180 190 200
DEIAHLKYKD PQSTKIPVLE QELVRAEAES LVAEAQLSNI TREKLKAAYS
210 220 230 240 250
YMFDSLRELS EKFALIAGYG KALLELLDDS PVTPGEARPA YDGYEASRQI
260 270 280 290 300
IMDAESALES WTLDMAAVKP TLSFHQTVDD VYEDEDGEEE EEPEIQNGDI
310 320 330 340
PGQVVEEEEV EWTTEVPVDD EAHEADHHVS QNGHTSGSEN I
Length:341
Mass (Da):38,071
Last modified:November 1, 1996 - v1
Checksum:i6FCEE022ECC9778C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U33335 Genomic DNA. Translation: AAB68101.1.
Z48483 Genomic DNA. Translation: CAA88382.1.
Z71255 Genomic DNA. Translation: CAA95037.1.
BK006949 Genomic DNA. Translation: DAA11424.1.
PIRiS52527.
RefSeqiNP_015321.1. NM_001183818.1.

Genome annotation databases

EnsemblFungiiYPL004C; YPL004C; YPL004C.
GeneIDi856103.
KEGGisce:YPL004C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U33335 Genomic DNA. Translation: AAB68101.1.
Z48483 Genomic DNA. Translation: CAA88382.1.
Z71255 Genomic DNA. Translation: CAA95037.1.
BK006949 Genomic DNA. Translation: DAA11424.1.
PIRiS52527.
RefSeqiNP_015321.1. NM_001183818.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3PLTX-ray2.90A/B/C36-267[»]
ProteinModelPortaliQ12230.
SMRiQ12230. Positions 51-264.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36173. 124 interactions.
DIPiDIP-3993N.
IntActiQ12230. 46 interactions.
MINTiMINT-546535.

PTM databases

iPTMnetiQ12230.

Proteomic databases

MaxQBiQ12230.
PRIDEiQ12230.
TopDownProteomicsiQ12230.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYPL004C; YPL004C; YPL004C.
GeneIDi856103.
KEGGisce:YPL004C.

Organism-specific databases

EuPathDBiFungiDB:YPL004C.
SGDiS000005925. LSP1.

Phylogenomic databases

GeneTreeiENSGT00390000012909.
HOGENOMiHOG000214828.
InParanoidiQ12230.
OMAiYSMRQTR.
OrthoDBiEOG092C3P1A.

Enzyme and pathway databases

BioCyciYEAST:G3O-33923-MONOMER.

Miscellaneous databases

EvolutionaryTraceiQ12230.
PROiQ12230.

Family and domain databases

InterProiIPR028245. PIL1/LSP1.
[Graphical view]
PfamiPF13805. Pil1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiLSP1_YEAST
AccessioniPrimary (citable) accession number: Q12230
Secondary accession number(s): D6W408
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 22, 2005
Last sequence update: November 1, 1996
Last modified: September 7, 2016
This is version 132 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 104485 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XVI
    Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.