Q12224 (RLM1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Transcription factor RLM1

Gene names

Name:	RLM1
Ordered Locus Names:	YPL089C
ORF Names:	LPG19C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	676 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May function as a transcription factor downstream of MPK1 that is subject to activation by the MPK1 mitogen-activated protein kinase pathway. Binds to the DNA sequence 5'-CTA[TA]4TAG-3'. At least some RML1 target genes are involved in cell wall biosynthesis. Ref.5
Subunit structure	Can heterodimerize with SPM1. Interacts with KDX1 and SLT2. Ref.5
Subcellular location	Nucleus Potential.
Post-translational modification	Phosphorylated by SLT2. Ref.5
Miscellaneous	Present with 736 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the MEF2 family. Contains 1 MADS-box domain. Contains 1 Mef2-type DNA-binding domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Ligand	DNA-binding
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	positive regulation of transcription from RNA polymerase II promoter in response to stress Inferred from direct assay PubMed 15116342. Source: SGD signal transduction Inferred from mutant phenotype Ref.1. Source: SGD
Cellular_component	nucleus Inferred from direct assay PubMed 12410835. Source: SGD
Molecular_function	DNA binding, bending Inferred from direct assay PubMed 10064699. Source: SGD RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity Inferred from direct assay Ref.4. Source: SGD sequence-specific DNA binding Inferred from direct assay Ref.4. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 676	676	Transcription factor RLM1		PRO_0000199440
Regions
	Domain	3 – 57	55	MADS-box
	DNA binding	58 – 87	30	Mef2-type Potential
	Compositional bias	129 – 134	6	Poly-Asp
	Compositional bias	207 – 212	6	Poly-Gln
	Compositional bias	229 – 236	8	Poly-Ser
	Compositional bias	414 – 417	4	Poly-Asn
	Compositional bias	567 – 579	13	Poly-Asn
Amino acid modifications
	Modified residue	164	1	Phosphoserine Ref.7
	Modified residue	370	1	Phosphoserine Ref.8
	Modified residue	371	1	Phosphoserine Ref.8
	Modified residue	374	1	Phosphoserine Ref.7 Ref.8
	Modified residue	377	1	Phosphoserine Ref.7 Ref.8

Sequences

	Sequence		Length	Mass (Da)
	Q12224 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 9CE6B3DE47632747	FASTA	676	73,484
	10 20 30 40 50 60 MGRRKIEIQR ISDDRNRAVT FIKRKAGLFK KAHELSVLCQ VDIAVIILGS NNTFYEFSSV 70 80 90 100 110 120 DTNDLIYHYQ NDKNLLHEVK DPSDYGDFHK SASVNINQDL LRSSMSNKPS KSNVKGMNQS 130 140 150 160 170 180 ENDDDENNDE DDDDHGNFER NSNMHSNKKA SDKNIPSAHM KLLSPTALIS KMDGSEQNKR 190 200 210 220 230 240 HPENALPPLQ HLKRLKPDPL QISRTPQQQQ QQNISRPYHS SMYNLNQPSS SSSSPSTMDF 250 260 270 280 290 300 PKLPSFQNSS FNGRPPPISI SPNKFSKPFT NASSRTPKQE HKINNSGSNN NDNSNYTQSP 310 320 330 340 350 360 SNSLEDSIQQ TVKARRKLSA RPVLRVRIPN NNFSSNSAIP SEPSSASSTS ANGNSMGSSQ 370 380 390 400 410 420 IMKENKTSRS SKISPLSASA SGPLTLQKGN NGRMVIKLPN ANAPNGSNNG NGSNNNNHPY 430 440 450 460 470 480 PFGSGSSPLF SATQPYIATP LQPSNIPGGP FQQNTSFLAQ RQTQQYQQMS FKKQSQTVPL 490 500 510 520 530 540 TTTLTGRPPS TFSGPETSNG PPTGSLPSKF VHDLMSNSPN VSSISMFPDW SMGPNSAKPG 550 560 570 580 590 600 NTNNPGTFPP VQTAVNNGNS SNISSTNNTN NNNNNNNNNS SNNNSNNGND NNSNNSNNSY 610 620 630 640 650 660 YSNNEDAPVN GAAISEHTTD GDSNNQSNSS TYDAAATAYN GNTGLTPYIN TAQTPLGTKF 670 FNFSTDISGE KNSSKI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Yeast RLM1 encodes a serum response factor-like protein that may function downstream of the Mpk1 (Slt2) mitogen-activated protein kinase pathway." Watanabe Y., Irie K., Matsumoto K. Mol. Cell. Biol. 15:5740-5749(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI." Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M. , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J. Nature 387:103-105(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"The Saccharomyces cerevisiae MADS-box transcription factor Rlm1 is a target for the Mpk1 mitogen-activated protein kinase pathway." Dodou E., Treisman R. Mol. Cell. Biol. 17:1848-1859(1997) [PubMed] [Europe PMC] [Abstract] Cited for: CHARACTERIZATION.
[5]	"Characterization of a serum response factor-like protein in Saccharomyces cerevisiae, Rlm1, which has transcriptional activity regulated by the Mpk1 (Slt2) mitogen-activated protein kinase pathway." Watanabe Y., Takaesu G., Hagiwara M., Irie K., Matsumoto K. Mol. Cell. Biol. 17:2615-2623(1997) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, PHOSPHORYLATION, INTERACTION WITH KDX1 AND SLT2.
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]	"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-164; SER-374 AND SER-377, MASS SPECTROMETRY. Strain: ADR376.
[8]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-370; SER-371; SER-374 AND SER-377, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U43281 Genomic DNA. Translation: AAB68210.1. D63340 Genomic DNA. Translation: BAA09658.1. BK006949 Genomic DNA. Translation: DAA11344.1.
PIR	S61977.
RefSeq	NP_015236.1. NM_001183903.1.
3D structure databases
ProteinModelPortal	Q12224.
SMR	Q12224. Positions 2-103.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-2480N.
IntAct	Q12224. 11 interactions.
MINT	MINT-2781402.
STRING	4932.YPL089C.
Proteomic databases
PaxDb	Q12224.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YPL089C; YPL089C; YPL089C.
GeneID	856016.
KEGG	sce:YPL089C.
Organism-specific databases
CYGD	YPL089c.
SGD	S000006010. RLM1.
Phylogenomic databases
eggNOG	COG5068.
GeneTree	ENSGT00390000011828.
KO	K09265.
OMA	NNTFYEF.
OrthoDB	EOG42NN89.
Enzyme and pathway databases
BioCyc	YEAST:G3O-33994-MONOMER.
Gene expression databases
Genevestigator	Q12224.
GermOnline	YPL089C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR002100. TF_MADSbox. [Graphical view]
Pfam	PF00319. SRF-TF. 1 hit. [Graphical view]
PRINTS	PR00404. MADSDOMAIN.
SMART	SM00432. MADS. 1 hit. [Graphical view]
SUPFAM	SSF55455. TF_MADSbox. 1 hit.
PROSITE	PS00350. MADS_BOX_1. 1 hit. PS50066. MADS_BOX_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	980914.

Entry information

Entry name

RLM1_YEAST

Accession

Primary (citable) accession number: Q12224
Secondary accession number(s): D6W3S8

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	November 1, 1996
Last modified:	May 29, 2013
This is version 107 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program