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Protein

Transcription factor RLM1

Gene

RLM1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May function as a transcription factor downstream of MPK1 that is subject to activation by the MPK1 mitogen-activated protein kinase pathway. Binds to the DNA sequence 5'-CTA[TA]4TAG-3'. At least some RML1 target genes are involved in cell wall biosynthesis.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi58 – 87Mef2-typeSequence analysisAdd BLAST30

GO - Molecular functioni

  • DNA binding, bending Source: SGD
  • mitogen-activated protein kinase binding Source: UniProtKB
  • RNA polymerase II regulatory region sequence-specific DNA binding Source: InterPro
  • sequence-specific DNA binding Source: SGD
  • transcription factor activity, RNA polymerase II core promoter proximal region sequence-specific binding Source: SGD

GO - Biological processi

  • MAPK cascade Source: InterPro
  • positive regulation of transcription from RNA polymerase II promoter Source: SGD
  • positive regulation of transcription from RNA polymerase II promoter in response to stress Source: SGD
  • signal transduction Source: SGD
  • transcription, DNA-templated Source: UniProtKB-KW

Keywordsi

Molecular functionDNA-binding
Biological processTranscription, Transcription regulation

Enzyme and pathway databases

BioCyciYEAST:G3O-33994-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription factor RLM1
Gene namesi
Name:RLM1
Ordered Locus Names:YPL089C
ORF Names:LPG19C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XVI

Organism-specific databases

EuPathDBiFungiDB:YPL089C.
SGDiS000006010. RLM1.

Subcellular locationi

  • Nucleus PROSITE-ProRule annotation

GO - Cellular componenti

  • nucleus Source: SGD

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001994401 – 676Transcription factor RLM1Add BLAST676

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei120PhosphoserineCombined sources1
Modified residuei164PhosphoserineCombined sources1
Modified residuei374PhosphoserineCombined sources1
Modified residuei377PhosphoserineCombined sources1

Post-translational modificationi

Phosphorylated by SLT2.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ12224.
PRIDEiQ12224.

PTM databases

iPTMnetiQ12224.

Interactioni

Subunit structurei

Can heterodimerize with SPM1. Interacts with KDX1 and SLT2.1 Publication

GO - Molecular functioni

  • mitogen-activated protein kinase binding Source: UniProtKB

Protein-protein interaction databases

BioGridi36092. 107 interactors.
DIPiDIP-2480N.
IntActiQ12224. 12 interactors.
MINTiMINT-2781402.

Structurei

3D structure databases

ProteinModelPortaliQ12224.
SMRiQ12224.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini3 – 57MADS-boxPROSITE-ProRule annotationAdd BLAST55

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi129 – 134Poly-Asp6
Compositional biasi207 – 212Poly-Gln6
Compositional biasi229 – 236Poly-Ser8
Compositional biasi414 – 417Poly-Asn4
Compositional biasi567 – 579Poly-AsnAdd BLAST13

Sequence similaritiesi

Belongs to the MEF2 family.Curated

Phylogenomic databases

GeneTreeiENSGT00390000011828.
InParanoidiQ12224.
KOiK09265.
OMAiGLTPYIN.
OrthoDBiEOG092C1PUJ.

Family and domain databases

CDDicd00265. MADS_MEF2_like. 1 hit.
InterProiView protein in InterPro
IPR033896. MADS_MEF2-like.
IPR002100. TF_MADSbox.
PfamiView protein in Pfam
PF00319. SRF-TF. 1 hit.
PRINTSiPR00404. MADSDOMAIN.
SMARTiView protein in SMART
SM00432. MADS. 1 hit.
SUPFAMiSSF55455. SSF55455. 1 hit.
PROSITEiView protein in PROSITE
PS00350. MADS_BOX_1. 1 hit.
PS50066. MADS_BOX_2. 1 hit.

Sequencei

Sequence statusi: Complete.

Q12224-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGRRKIEIQR ISDDRNRAVT FIKRKAGLFK KAHELSVLCQ VDIAVIILGS
60 70 80 90 100
NNTFYEFSSV DTNDLIYHYQ NDKNLLHEVK DPSDYGDFHK SASVNINQDL
110 120 130 140 150
LRSSMSNKPS KSNVKGMNQS ENDDDENNDE DDDDHGNFER NSNMHSNKKA
160 170 180 190 200
SDKNIPSAHM KLLSPTALIS KMDGSEQNKR HPENALPPLQ HLKRLKPDPL
210 220 230 240 250
QISRTPQQQQ QQNISRPYHS SMYNLNQPSS SSSSPSTMDF PKLPSFQNSS
260 270 280 290 300
FNGRPPPISI SPNKFSKPFT NASSRTPKQE HKINNSGSNN NDNSNYTQSP
310 320 330 340 350
SNSLEDSIQQ TVKARRKLSA RPVLRVRIPN NNFSSNSAIP SEPSSASSTS
360 370 380 390 400
ANGNSMGSSQ IMKENKTSRS SKISPLSASA SGPLTLQKGN NGRMVIKLPN
410 420 430 440 450
ANAPNGSNNG NGSNNNNHPY PFGSGSSPLF SATQPYIATP LQPSNIPGGP
460 470 480 490 500
FQQNTSFLAQ RQTQQYQQMS FKKQSQTVPL TTTLTGRPPS TFSGPETSNG
510 520 530 540 550
PPTGSLPSKF VHDLMSNSPN VSSISMFPDW SMGPNSAKPG NTNNPGTFPP
560 570 580 590 600
VQTAVNNGNS SNISSTNNTN NNNNNNNNNS SNNNSNNGND NNSNNSNNSY
610 620 630 640 650
YSNNEDAPVN GAAISEHTTD GDSNNQSNSS TYDAAATAYN GNTGLTPYIN
660 670
TAQTPLGTKF FNFSTDISGE KNSSKI
Length:676
Mass (Da):73,484
Last modified:November 1, 1996 - v1
Checksum:i9CE6B3DE47632747
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U43281 Genomic DNA. Translation: AAB68210.1.
D63340 Genomic DNA. Translation: BAA09658.1.
BK006949 Genomic DNA. Translation: DAA11344.1.
PIRiS61977.
RefSeqiNP_015236.1. NM_001183903.1.

Genome annotation databases

EnsemblFungiiYPL089C; YPL089C; YPL089C.
GeneIDi856016.
KEGGisce:YPL089C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U43281 Genomic DNA. Translation: AAB68210.1.
D63340 Genomic DNA. Translation: BAA09658.1.
BK006949 Genomic DNA. Translation: DAA11344.1.
PIRiS61977.
RefSeqiNP_015236.1. NM_001183903.1.

3D structure databases

ProteinModelPortaliQ12224.
SMRiQ12224.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36092. 107 interactors.
DIPiDIP-2480N.
IntActiQ12224. 12 interactors.
MINTiMINT-2781402.

PTM databases

iPTMnetiQ12224.

Proteomic databases

MaxQBiQ12224.
PRIDEiQ12224.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYPL089C; YPL089C; YPL089C.
GeneIDi856016.
KEGGisce:YPL089C.

Organism-specific databases

EuPathDBiFungiDB:YPL089C.
SGDiS000006010. RLM1.

Phylogenomic databases

GeneTreeiENSGT00390000011828.
InParanoidiQ12224.
KOiK09265.
OMAiGLTPYIN.
OrthoDBiEOG092C1PUJ.

Enzyme and pathway databases

BioCyciYEAST:G3O-33994-MONOMER.

Miscellaneous databases

PROiQ12224.

Family and domain databases

CDDicd00265. MADS_MEF2_like. 1 hit.
InterProiView protein in InterPro
IPR033896. MADS_MEF2-like.
IPR002100. TF_MADSbox.
PfamiView protein in Pfam
PF00319. SRF-TF. 1 hit.
PRINTSiPR00404. MADSDOMAIN.
SMARTiView protein in SMART
SM00432. MADS. 1 hit.
SUPFAMiSSF55455. SSF55455. 1 hit.
PROSITEiView protein in PROSITE
PS00350. MADS_BOX_1. 1 hit.
PS50066. MADS_BOX_2. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiRLM1_YEAST
AccessioniPrimary (citable) accession number: Q12224
Secondary accession number(s): D6W3S8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: November 1, 1996
Last modified: March 15, 2017
This is version 138 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 736 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XVI
    Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.