UniProtKB - Q12224 (RLM1_YEAST)
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Protein
Transcription factor RLM1
Gene
RLM1
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
May function as a transcription factor downstream of MPK1 that is subject to activation by the MPK1 mitogen-activated protein kinase pathway. Binds to the DNA sequence 5'-CTA[TA]4TAG-3'. At least some RML1 target genes are involved in cell wall biosynthesis.1 Publication
Miscellaneous
Present with 736 molecules/cell in log phase SD medium.1 Publication
Regions
| Feature key | Position(s) | DescriptionActions | Graphical view | Length |
|---|---|---|---|---|
| DNA bindingi | 58 – 87 | Mef2-typeSequence analysisAdd BLAST | 30 |
GO - Molecular functioni
- DNA binding, bending Source: SGD
- mitogen-activated protein kinase binding Source: UniProtKB
- protein dimerization activity Source: InterPro
- RNA polymerase II regulatory region sequence-specific DNA binding Source: InterPro
- sequence-specific DNA binding Source: SGD
- transcription factor activity, RNA polymerase II core promoter proximal region sequence-specific binding Source: SGD
GO - Biological processi
- positive regulation of transcription from RNA polymerase II promoter Source: SGD
- positive regulation of transcription from RNA polymerase II promoter in response to stress Source: SGD
- signal transduction Source: SGD
- transcription, DNA-templated Source: UniProtKB-KW
Keywordsi
| Molecular function | DNA-binding |
| Biological process | Transcription, Transcription regulation |
Enzyme and pathway databases
| BioCyci | YEAST:G3O-33994-MONOMER. |
| Reactomei | R-SCE-375170. CDO in myogenesis. |
Names & Taxonomyi
| Protein namesi | Recommended name: Transcription factor RLM1 |
| Gene namesi | Name:RLM1 Ordered Locus Names:YPL089C ORF Names:LPG19C |
| Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
| Taxonomic identifieri | 559292 [NCBI] |
| Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
| Proteomesi |
|
Organism-specific databases
| EuPathDBi | FungiDB:YPL089C. |
| SGDi | S000006010. RLM1. |
Subcellular locationi
- Nucleus PROSITE-ProRule annotation
GO - Cellular componenti
- nucleus Source: SGD
Keywords - Cellular componenti
NucleusPTM / Processingi
Molecule processing
| Feature key | Position(s) | DescriptionActions | Graphical view | Length |
|---|---|---|---|---|
| ChainiPRO_0000199440 | 1 – 676 | Transcription factor RLM1Add BLAST | 676 |
Amino acid modifications
| Feature key | Position(s) | DescriptionActions | Graphical view | Length |
|---|---|---|---|---|
| Modified residuei | 120 | PhosphoserineCombined sources | 1 | |
| Modified residuei | 164 | PhosphoserineCombined sources | 1 | |
| Modified residuei | 374 | PhosphoserineCombined sources | 1 | |
| Modified residuei | 377 | PhosphoserineCombined sources | 1 |
Post-translational modificationi
Phosphorylated by SLT2.1 Publication
Keywords - PTMi
PhosphoproteinProteomic databases
| MaxQBi | Q12224. |
| PRIDEi | Q12224. |
PTM databases
| iPTMneti | Q12224. |
Interactioni
Subunit structurei
Can heterodimerize with SPM1. Interacts with KDX1 and SLT2.1 Publication
GO - Molecular functioni
- mitogen-activated protein kinase binding Source: UniProtKB
- protein dimerization activity Source: InterPro
Protein-protein interaction databases
| BioGridi | 36092. 107 interactors. |
| DIPi | DIP-2480N. |
| IntActi | Q12224. 12 interactors. |
| MINTi | MINT-2781402. |
| STRINGi | 4932.YPL089C. |
Structurei
3D structure databases
| ProteinModelPortali | Q12224. |
| SMRi | Q12224. |
| ModBasei | Search... |
| MobiDBi | Search... |
Family & Domainsi
Domains and Repeats
| Feature key | Position(s) | DescriptionActions | Graphical view | Length |
|---|---|---|---|---|
| Domaini | 3 – 57 | MADS-boxPROSITE-ProRule annotationAdd BLAST | 55 |
Compositional bias
| Feature key | Position(s) | DescriptionActions | Graphical view | Length |
|---|---|---|---|---|
| Compositional biasi | 129 – 134 | Poly-Asp | 6 | |
| Compositional biasi | 207 – 212 | Poly-Gln | 6 | |
| Compositional biasi | 229 – 236 | Poly-Ser | 8 | |
| Compositional biasi | 414 – 417 | Poly-Asn | 4 | |
| Compositional biasi | 567 – 579 | Poly-AsnAdd BLAST | 13 |
Sequence similaritiesi
Belongs to the MEF2 family.Curated
Phylogenomic databases
| GeneTreei | ENSGT00390000011828. |
| InParanoidi | Q12224. |
| KOi | K09265. |
| OMAi | GLTPYIN. |
| OrthoDBi | EOG092C1PUJ. |
Family and domain databases
| CDDi | cd00265. MADS_MEF2_like. 1 hit. |
| Gene3Di | 3.40.1810.10. 1 hit. |
| InterProi | View protein in InterPro IPR033896. MADS_MEF2-like. IPR002100. TF_MADSbox. |
| Pfami | View protein in Pfam PF00319. SRF-TF. 1 hit. |
| PRINTSi | PR00404. MADSDOMAIN. |
| SMARTi | View protein in SMART SM00432. MADS. 1 hit. |
| SUPFAMi | SSF55455. SSF55455. 1 hit. |
| PROSITEi | View protein in PROSITE PS00350. MADS_BOX_1. 1 hit. PS50066. MADS_BOX_2. 1 hit. |
Sequencei
Sequence statusi: Complete.
Q12224-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MGRRKIEIQR ISDDRNRAVT FIKRKAGLFK KAHELSVLCQ VDIAVIILGS
60 70 80 90 100
NNTFYEFSSV DTNDLIYHYQ NDKNLLHEVK DPSDYGDFHK SASVNINQDL
110 120 130 140 150
LRSSMSNKPS KSNVKGMNQS ENDDDENNDE DDDDHGNFER NSNMHSNKKA
160 170 180 190 200
SDKNIPSAHM KLLSPTALIS KMDGSEQNKR HPENALPPLQ HLKRLKPDPL
210 220 230 240 250
QISRTPQQQQ QQNISRPYHS SMYNLNQPSS SSSSPSTMDF PKLPSFQNSS
260 270 280 290 300
FNGRPPPISI SPNKFSKPFT NASSRTPKQE HKINNSGSNN NDNSNYTQSP
310 320 330 340 350
SNSLEDSIQQ TVKARRKLSA RPVLRVRIPN NNFSSNSAIP SEPSSASSTS
360 370 380 390 400
ANGNSMGSSQ IMKENKTSRS SKISPLSASA SGPLTLQKGN NGRMVIKLPN
410 420 430 440 450
ANAPNGSNNG NGSNNNNHPY PFGSGSSPLF SATQPYIATP LQPSNIPGGP
460 470 480 490 500
FQQNTSFLAQ RQTQQYQQMS FKKQSQTVPL TTTLTGRPPS TFSGPETSNG
510 520 530 540 550
PPTGSLPSKF VHDLMSNSPN VSSISMFPDW SMGPNSAKPG NTNNPGTFPP
560 570 580 590 600
VQTAVNNGNS SNISSTNNTN NNNNNNNNNS SNNNSNNGND NNSNNSNNSY
610 620 630 640 650
YSNNEDAPVN GAAISEHTTD GDSNNQSNSS TYDAAATAYN GNTGLTPYIN
660 670
TAQTPLGTKF FNFSTDISGE KNSSKI
Sequence databases
| Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U43281 Genomic DNA. Translation: AAB68210.1. D63340 Genomic DNA. Translation: BAA09658.1. BK006949 Genomic DNA. Translation: DAA11344.1. |
| PIRi | S61977. |
| RefSeqi | NP_015236.1. NM_001183903.1. |
Genome annotation databases
| EnsemblFungii | YPL089C; YPL089C; YPL089C. |
| GeneIDi | 856016. |
| KEGGi | sce:YPL089C. |
Similar proteinsi
Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:| 100% | UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry. |
| 90% | UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence). |
| 50% | UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster. |
Entry informationi
| Entry namei | RLM1_YEAST | |
| Accessioni | Q12224Primary (citable) accession number: Q12224 Secondary accession number(s): D6W3S8 | |
| Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 15, 1998 |
| Last sequence update: | November 1, 1996 | |
| Last modified: | July 5, 2017 | |
| This is version 141 of the entry and version 1 of the sequence. See complete history. | ||
| Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
| Annotation program | Fungal Protein Annotation Program | |
Miscellaneousi
Keywords - Technical termi
Complete proteome, Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families - Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome XVI
Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names
