Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

mRNA-binding protein PUF2

Gene

PUF2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

RNA-binding protein involved in post-transcriptional regulation. Negatively regulates expression of COX17 by binding to the 3'-UTR of COX17 mRNA. Promotes decay of COX17 mRNA by enhancing its rate of deadenylation and subsequent turnover. Predominantly binds to mRNAs encoding membrane-associated proteins with roles in transmembrane transport and vesicular trafficking.1 Publication

Miscellaneous

Present with 377 molecules/cell in log phase SD medium.1 Publication

GO - Molecular functioni

  • mRNA binding Source: SGD

GO - Biological processi

  • nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay Source: SGD

Keywordsi

Molecular functionRNA-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-34198-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
mRNA-binding protein PUF2
Alternative name(s):
Pumilio homology domain family member 2
Gene namesi
Name:PUF2
Ordered Locus Names:YPR042C
ORF Names:YP3085.06c, YP9499.01
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XVI

Organism-specific databases

EuPathDBiFungiDB:YPR042C
SGDiS000006246 PUF2

Subcellular locationi

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002627541 – 1075mRNA-binding protein PUF2Add BLAST1075

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei72PhosphoserineCombined sources1
Modified residuei198PhosphoserineCombined sources1
Modified residuei872PhosphoserineCombined sources1
Modified residuei876PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ12221
PaxDbiQ12221
PRIDEiQ12221

PTM databases

iPTMnetiQ12221

Interactioni

Protein-protein interaction databases

BioGridi36219, 1279 interactors
IntActiQ12221, 9 interactors
MINTiQ12221
STRINGi4932.YPR042C

Structurei

3D structure databases

ProteinModelPortaliQ12221
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini316 – 402RRMPROSITE-ProRule annotationAdd BLAST87
Domaini511 – 872PUM-HDPROSITE-ProRule annotationAdd BLAST362
Repeati574 – 611Pumilio 1Add BLAST38
Repeati612 – 647Pumilio 2Add BLAST36
Repeati649 – 683Pumilio 3Add BLAST35
Repeati684 – 719Pumilio 4Add BLAST36
Repeati722 – 758Pumilio 5Add BLAST37
Repeati760 – 800Pumilio 6Add BLAST41

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi910 – 1063Asn-richAdd BLAST154

Keywords - Domaini

Repeat

Phylogenomic databases

GeneTreeiENSGT00730000112282
HOGENOMiHOG000113136
InParanoidiQ12221
OrthoDBiEOG092C1612

Family and domain databases

Gene3Di1.25.10.10, 1 hit
3.30.70.330, 1 hit
InterProiView protein in InterPro
IPR011989 ARM-like
IPR016024 ARM-type_fold
IPR012677 Nucleotide-bd_a/b_plait_sf
IPR033133 PUM-HD
IPR001313 Pumilio_RNA-bd_rpt
IPR035979 RBD_domain_sf
IPR000504 RRM_dom
PfamiView protein in Pfam
PF00806 PUF, 3 hits
PF00076 RRM_1, 1 hit
SMARTiView protein in SMART
SM00025 Pumilio, 6 hits
SM00360 RRM, 1 hit
SUPFAMiSSF48371 SSF48371, 1 hit
SSF54928 SSF54928, 1 hit
PROSITEiView protein in PROSITE
PS50302 PUM, 5 hits
PS50303 PUM_HD, 1 hit
PS50102 RRM, 1 hit

Sequencei

Sequence statusi: Complete.

Q12221-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDNKRLYNGN LSNIPEVIDP GITIPIYEED IRNDTRMNTN ARSVRVSDKR
60 70 80 90 100
GRSSSTSPQK IGSYRTRAGR FSDTLTNLLP SISAKLHHSK KSTPVVVVPP
110 120 130 140 150
TSSTPDSLNS TTYAPRVSSD SFTVATPLSL QSTTTRTRTR NNTVSSQITA
160 170 180 190 200
SSSLTTDVGN ATSANIWSAN AESNTSSSPL FDYPLATSYF EPLTRFKSTD
210 220 230 240 250
NYTLPQTAQL NSFLEKNGNP NIWSSAGNSN TDHLNTPIVN RQRSQSQSTT
260 270 280 290 300
NRVYTDAPYY QQPAQNYQVQ VPPRVPKSTS ISPVILDDVD PASINWITAN
310 320 330 340 350
QKVPLVNQIS ALLPTNTISI SNVFPLQPTQ QHQQNAVNLT STSLATLCSQ
360 370 380 390 400
YGKVLSARTL RGLNMALVEF STVESAICAL EALQGKELSK VGAPSTVSFA
410 420 430 440 450
RVLPMYEQPL NVNGFNNTPK QPLLQEQLNH GVLNYQLQQS LQQPELQQQP
460 470 480 490 500
TSFNQPNLTY CNPTQNLSHL QLSSNENEPY PFPLPPPSLS DSKKDILHTI
510 520 530 540 550
SSFKLEYDHL ELNHLLQNAL KNKGVSDTNY FGPLPEHNSK VPKRKDTFDA
560 570 580 590 600
PKLRELRKQF DSNSLSTIEM EQLAIVMLDQ LPELSSDYLG NTVIQKLFEN
610 620 630 640 650
SSNIIRDIML RKCNKYLTSM GVHKNGTWVC QKIIKMANTP RQINLVTSGV
660 670 680 690 700
SDYCTPLFND QFGNYVIQGI LKFGFPWNSF IFESVLSHFW TIVQNRYGSR
710 720 730 740 750
AVRACLEADS IITQCQLLTI TSLIIVLSPY LATDTNGTLL ITWLLDTCTL
760 770 780 790 800
PNKNLILCDK LVNKNLVKLC CHKLGSLTVL KILNLRGGEE EALSKNKIIH
810 820 830 840 850
AIFDGPISSD SILFQILDEG NYGPTFIYKV LTSRILDNSV RDEAITKIRQ
860 870 880 890 900
LILNSNINLQ SRQLLEEVGL SSAGISPKQS SKNHRKQHPQ GFHSPGRARG
910 920 930 940 950
VSVSSVRSSN SRHNSVIQMN NAGPTPALNF NPAPMSEINS YFNNQQVVYS
960 970 980 990 1000
GNQNQNQNGN SNGLDELNSQ FDSFRIANGT NLSLPIVNLP NVSNNNNNYN
1010 1020 1030 1040 1050
NSGYSSQMNP LSRSVSHNNN NNTNNYNNND NDNNNNNNNN NNNNNNNNNN
1060 1070
NNNSNNSNNN NNNDTSLYRY RSYGY
Length:1,075
Mass (Da):119,508
Last modified:November 1, 1996 - v1
Checksum:iCD3E4D5FE9B2F47D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z73616 Genomic DNA Translation: CAA97992.1
Z71255 Genomic DNA Translation: CAA94990.1
Z49219 Genomic DNA Translation: CAA89163.1
Z68111 Genomic DNA Translation: CAA92146.1
BK006949 Genomic DNA Translation: DAA11467.1
PIRiS54067
RefSeqiNP_015367.1, NM_001184139.1

Genome annotation databases

EnsemblFungiiYPR042C; YPR042C; YPR042C
GeneIDi856155
KEGGisce:YPR042C

Similar proteinsi

Entry informationi

Entry nameiPUF2_YEAST
AccessioniPrimary (citable) accession number: Q12221
Secondary accession number(s): D6W451
, Q02573, Q04142, Q7LH42, Q7LH98
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 28, 2006
Last sequence update: November 1, 1996
Last modified: May 23, 2018
This is version 137 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  2. Yeast chromosome XVI
    Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

Cookie policy

We would like to use anonymized google analytics cookies to gather statistics on how uniprot.org is used in aggregate. Learn more

UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health