ID   UTP12_YEAST             Reviewed;         943 AA.
AC   Q12220; D6VYC4; Q05386;
DT   15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   27-MAR-2024, entry version 189.
DE   RecName: Full=U3 small nucleolar RNA-associated protein 12;
DE            Short=U3 snoRNA-associated protein 12;
DE   AltName: Full=DOM34-interacting protein 2;
DE   AltName: Full=U three protein 12;
GN   Name=DIP2; Synonyms=UTP12; OrderedLocusNames=YLR129W;
GN   ORFNames=L3116, L9233.1;
OS   Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX   NCBI_TaxID=559292;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=9169871;
RA   Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W.,
RA   Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A.,
RA   Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K.,
RA   Heuss-Neitzel D., Hilbert H., Hilger F., Kleine K., Koetter P., Louis E.J.,
RA   Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S.,
RA   Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D.,
RA   Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M.,
RA   Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P.,
RA   Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M.,
RA   Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K.,
RA   Zollner A., Hani J., Hoheisel J.D.;
RT   "The nucleotide sequence of Saccharomyces cerevisiae chromosome XII.";
RL   Nature 387:87-90(1997).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=24374639; DOI=10.1534/g3.113.008995;
RA   Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA   Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA   Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT   "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL   G3 (Bethesda) 4:389-398(2014).
RN   [3]
RP   FUNCTION, INTERACTION WITH MPP10 AND SNORNA U3, IDENTIFICATION IN SSU
RP   PROCESSOME BY MASS SPECTROMETRY, AND SUBCELLULAR LOCATION.
RX   PubMed=12068309; DOI=10.1038/nature00769;
RA   Dragon F., Gallagher J.E.G., Compagnone-Post P.A., Mitchell B.M.,
RA   Porwancher K.A., Wehner K.A., Wormsley S., Settlage R.E., Shabanowitz J.,
RA   Osheim Y., Beyer A.L., Hunt D.F., Baserga S.J.;
RT   "A large nucleolar U3 ribonucleoprotein required for 18S ribosomal RNA
RT   biogenesis.";
RL   Nature 417:967-970(2002).
RN   [4]
RP   LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX   PubMed=14562106; DOI=10.1038/nature02046;
RA   Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA   O'Shea E.K., Weissman J.S.;
RT   "Global analysis of protein expression in yeast.";
RL   Nature 425:737-741(2003).
CC   -!- FUNCTION: Involved in nucleolar processing of pre-18S ribosomal RNA.
CC       {ECO:0000269|PubMed:12068309}.
CC   -!- SUBUNIT: Interacts with snoRNA U3. Interacts with MPP10. Component of
CC       the ribosomal small subunit (SSU) processome composed of at least 40
CC       protein subunits and snoRNA U3. {ECO:0000269|PubMed:12068309}.
CC   -!- INTERACTION:
CC       Q12220; P25635: PWP2; NbExp=11; IntAct=EBI-5896, EBI-14332;
CC       Q12220; Q05946: UTP13; NbExp=7; IntAct=EBI-5896, EBI-34702;
CC   -!- SUBCELLULAR LOCATION: Nucleus, nucleolus {ECO:0000269|PubMed:12068309}.
CC   -!- MISCELLANEOUS: Present with 9620 molecules/cell in log phase SD medium.
CC       {ECO:0000269|PubMed:14562106}.
CC   -!- SIMILARITY: Belongs to the WD repeat WDR3/UTP12 family. {ECO:0000305}.
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DR   EMBL; U53877; AAB82375.1; -; Genomic_DNA.
DR   EMBL; U53881; AAB82402.1; -; Genomic_DNA.
DR   EMBL; X91258; CAA62640.1; -; Genomic_DNA.
DR   EMBL; Z73301; CAA97699.1; -; Genomic_DNA.
DR   EMBL; Z73302; CAA97700.1; -; Genomic_DNA.
DR   EMBL; X89514; CAA61707.1; -; Genomic_DNA.
DR   EMBL; BK006945; DAA09440.1; -; Genomic_DNA.
DR   PIR; S59317; S59317.
DR   RefSeq; NP_013230.1; NM_001182016.1.
DR   PDB; 5WLC; EM; 3.80 A; LQ=1-943.
DR   PDB; 5WYJ; EM; 8.70 A; BB=1-943.
DR   PDB; 5WYK; EM; 4.50 A; BB=1-943.
DR   PDB; 6KE6; EM; 3.40 A; B2=1-943.
DR   PDB; 6LQP; EM; 3.20 A; B2=1-943.
DR   PDB; 6LQQ; EM; 4.10 A; B2=1-943.
DR   PDB; 6LQR; EM; 8.60 A; B2=1-943.
DR   PDB; 6LQS; EM; 3.80 A; B2=1-943.
DR   PDB; 6LQT; EM; 4.90 A; B2=1-943.
DR   PDB; 6LQU; EM; 3.70 A; B2=1-943.
DR   PDB; 6LQV; EM; 4.80 A; B2=1-943.
DR   PDB; 6ND4; EM; 4.30 A; Q=1-943.
DR   PDB; 6ZQA; EM; 4.40 A; UL=1-943.
DR   PDB; 6ZQB; EM; 3.90 A; UL=1-943.
DR   PDB; 6ZQC; EM; 3.80 A; UL=1-943.
DR   PDB; 6ZQD; EM; 3.80 A; UL=1-943.
DR   PDB; 6ZQE; EM; 7.10 A; UL=1-943.
DR   PDB; 6ZQF; EM; 4.90 A; UL=1-943.
DR   PDB; 7AJT; EM; 4.60 A; UL=1-943.
DR   PDB; 7AJU; EM; 3.80 A; UL=1-943.
DR   PDB; 7D4I; EM; 4.00 A; B2=1-943.
DR   PDB; 7D5S; EM; 4.60 A; B2=1-943.
DR   PDB; 7D5T; EM; 6.00 A; B2=1-943.
DR   PDB; 7D63; EM; 12.30 A; B2=1-943.
DR   PDB; 7SUK; EM; 3.99 A; LQ=5-943.
DR   PDBsum; 5WLC; -.
DR   PDBsum; 5WYJ; -.
DR   PDBsum; 5WYK; -.
DR   PDBsum; 6KE6; -.
DR   PDBsum; 6LQP; -.
DR   PDBsum; 6LQQ; -.
DR   PDBsum; 6LQR; -.
DR   PDBsum; 6LQS; -.
DR   PDBsum; 6LQT; -.
DR   PDBsum; 6LQU; -.
DR   PDBsum; 6LQV; -.
DR   PDBsum; 6ND4; -.
DR   PDBsum; 6ZQA; -.
DR   PDBsum; 6ZQB; -.
DR   PDBsum; 6ZQC; -.
DR   PDBsum; 6ZQD; -.
DR   PDBsum; 6ZQE; -.
DR   PDBsum; 6ZQF; -.
DR   PDBsum; 7AJT; -.
DR   PDBsum; 7AJU; -.
DR   PDBsum; 7D4I; -.
DR   PDBsum; 7D5S; -.
DR   PDBsum; 7D5T; -.
DR   PDBsum; 7D63; -.
DR   PDBsum; 7SUK; -.
DR   AlphaFoldDB; Q12220; -.
DR   EMDB; EMD-0949; -.
DR   EMDB; EMD-0950; -.
DR   EMDB; EMD-0951; -.
DR   EMDB; EMD-0952; -.
DR   EMDB; EMD-0953; -.
DR   EMDB; EMD-0954; -.
DR   EMDB; EMD-0955; -.
DR   EMDB; EMD-11357; -.
DR   EMDB; EMD-11358; -.
DR   EMDB; EMD-11359; -.
DR   EMDB; EMD-11360; -.
DR   EMDB; EMD-11361; -.
DR   EMDB; EMD-11362; -.
DR   EMDB; EMD-11807; -.
DR   EMDB; EMD-11808; -.
DR   EMDB; EMD-30574; -.
DR   EMDB; EMD-30584; -.
DR   EMDB; EMD-30585; -.
DR   EMDB; EMD-30588; -.
DR   EMDB; EMD-6695; -.
DR   EMDB; EMD-6696; -.
DR   EMDB; EMD-9964; -.
DR   SMR; Q12220; -.
DR   BioGRID; 31398; 147.
DR   ComplexPortal; CPX-1410; UTP-B complex.
DR   DIP; DIP-6494N; -.
DR   IntAct; Q12220; 21.
DR   MINT; Q12220; -.
DR   STRING; 4932.YLR129W; -.
DR   iPTMnet; Q12220; -.
DR   MaxQB; Q12220; -.
DR   PaxDb; 4932-YLR129W; -.
DR   PeptideAtlas; Q12220; -.
DR   EnsemblFungi; YLR129W_mRNA; YLR129W; YLR129W.
DR   GeneID; 850820; -.
DR   KEGG; sce:YLR129W; -.
DR   AGR; SGD:S000004119; -.
DR   SGD; S000004119; DIP2.
DR   VEuPathDB; FungiDB:YLR129W; -.
DR   eggNOG; KOG0306; Eukaryota.
DR   GeneTree; ENSGT00940000153859; -.
DR   HOGENOM; CLU_005318_0_1_1; -.
DR   InParanoid; Q12220; -.
DR   OMA; MQCLRTV; -.
DR   OrthoDB; 168440at2759; -.
DR   BioCyc; YEAST:G3O-32271-MONOMER; -.
DR   Reactome; R-SCE-6791226; Major pathway of rRNA processing in the nucleolus and cytosol.
DR   BioGRID-ORCS; 850820; 4 hits in 10 CRISPR screens.
DR   PRO; PR:Q12220; -.
DR   Proteomes; UP000002311; Chromosome XII.
DR   RNAct; Q12220; Protein.
DR   GO; GO:0030686; C:90S preribosome; HDA:SGD.
DR   GO; GO:0005730; C:nucleolus; IDA:SGD.
DR   GO; GO:0005654; C:nucleoplasm; TAS:Reactome.
DR   GO; GO:0034388; C:Pwp2p-containing subcomplex of 90S preribosome; IDA:SGD.
DR   GO; GO:0032040; C:small-subunit processome; IDA:SGD.
DR   GO; GO:0030515; F:snoRNA binding; IBA:GO_Central.
DR   GO; GO:0034511; F:U3 snoRNA binding; IDA:SGD.
DR   GO; GO:0000480; P:endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA); IMP:SGD.
DR   GO; GO:0000447; P:endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA); IMP:SGD.
DR   GO; GO:0000472; P:endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA); IMP:SGD.
DR   GO; GO:0030490; P:maturation of SSU-rRNA; IBA:GO_Central.
DR   GO; GO:0000462; P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA); IMP:SGD.
DR   CDD; cd00200; WD40; 1.
DR   Gene3D; 2.130.10.10; YVTN repeat-like/Quinoprotein amine dehydrogenase; 4.
DR   InterPro; IPR020472; G-protein_beta_WD-40_rep.
DR   InterPro; IPR007148; SSU_processome_Utp12.
DR   InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR   InterPro; IPR019775; WD40_repeat_CS.
DR   InterPro; IPR036322; WD40_repeat_dom_sf.
DR   InterPro; IPR001680; WD40_rpt.
DR   PANTHER; PTHR19853; WD REPEAT CONTAINING PROTEIN 3 WDR3; 1.
DR   PANTHER; PTHR19853:SF0; WD REPEAT-CONTAINING PROTEIN 3; 1.
DR   Pfam; PF04003; Utp12; 1.
DR   Pfam; PF00400; WD40; 10.
DR   PRINTS; PR00320; GPROTEINBRPT.
DR   SMART; SM00320; WD40; 11.
DR   SUPFAM; SSF117289; Nucleoporin domain; 1.
DR   SUPFAM; SSF50978; WD40 repeat-like; 1.
DR   PROSITE; PS00678; WD_REPEATS_1; 4.
DR   PROSITE; PS50082; WD_REPEATS_2; 8.
DR   PROSITE; PS50294; WD_REPEATS_REGION; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Nucleus; Reference proteome; Repeat; Ribonucleoprotein;
KW   Ribosome biogenesis; rRNA processing; WD repeat.
FT   CHAIN           1..943
FT                   /note="U3 small nucleolar RNA-associated protein 12"
FT                   /id="PRO_0000050955"
FT   REPEAT          77..107
FT                   /note="WD 1"
FT   REPEAT          119..149
FT                   /note="WD 2"
FT   REPEAT          161..190
FT                   /note="WD 3"
FT   REPEAT          202..230
FT                   /note="WD 4"
FT   REPEAT          389..418
FT                   /note="WD 5"
FT   REPEAT          428..458
FT                   /note="WD 6"
FT   REPEAT          471..501
FT                   /note="WD 7"
FT   REPEAT          571..601
FT                   /note="WD 8"
FT   REPEAT          613..643
FT                   /note="WD 9"
FT   REPEAT          655..685
FT                   /note="WD 10"
FT   REGION          715..739
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        718..739
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   943 AA;  106342 MW;  7D9AEC7AF6A9C740 CRC64;
     MVKSYQRFEQ AAAFGVIASN ANCVWIPASS GNSNGSGPGQ LITSALEDVN IWDIKTGDLV
     SKLSDGLPPG ASDARGAKPA ECTYLEAHKD TDLLAVGYAD GVIKVWDLMS KTVLLNFNGH
     KAAITLLQFD GTGTRLISGS KDSNIIVWDL VGEVGLYKLR SHKDSITGFW CQGEDWLIST
     SKDGMIKLWD LKTHQCIETH IAHTGECWGL AVKDDLLITT GTDSQVKIWK LDIENDKMGG
     KLTEMGIFEK QSKQRGLKIE FITNSSDKTS FFYIQNADKT IETFRIRKEE EIARGLKKRE
     KRLKEKGLTE EEIAKSIKES YSSFILHPFQ TIRSLYKIKS ASWTTVSSSK LELVLTTSSN
     TIEYYSIPYE KRDPTSPAPL KTHTIELQGQ RTDVRSIDIS DDNKLLATAS NGSLKIWNIK
     THKCIRTFEC GYALTCKFLP GGLLVILGTR NGELQLFDLA SSSLLDTIED AHDAAIWSLD
     LTSDGKRLVT GSADKTVKFW DFKVENSLVP GTKNKFLPVL KLHHDTTLEL TDDILCVRVS
     PDDRYLAISL LDNTVKVFFL DSMKFYLSLY GHKLPVLSID ISFDSKMIIT SSADKNIKIW
     GLDFGDCHKS LFAHQDSIMN VKFLPQSHNF FSCSKDAVVK YWDGEKFECI QKLYAHQSEV
     WALAVATDGG FVVSSSHDHS IRIWEETEDQ VFLEEEKEKE LEEQYEDTLL TSLEEGNGDD
     AFKADASGEG VEDEASGVHK QTLESLKAGE RLMEALDLGI AEIEGLEAYN RDMKLWQRKK
     LGEAPIKPQG NAVLIAVNKT PEQYIMDTLL RIRMSQLEDA LMVMPFSYVL KFLKFIDTVM
     QNKTLLHSHL PLICKNLFFI IKFNHKELVS QKNEELKLQI NRVKTELRSA LKSTEDDLGF
     NVQGLKFVKQ QWNLRHNYEF VDEYDQQEKE SNSARKRVFG TVI
//