Reviewed, UniProtKB/Swiss-Prot Q12220 (UTP12_YEAST)

Names and origin

Protein names

Recommended name:
    U3 small nucleolar RNA-associated protein 12
      Short name=U3 snoRNA-associated protein 12
Alternative name(s):
    U three protein 12
    DOM34-interacting protein 2

Gene names

Name:	DIP2
Synonyms:	UTP12
Ordered Locus Names:	YLR129W
ORF Names:	L9233.1, L3116

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	943 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Involved in nucleolar processing of pre-18S ribosomal RNA. Ref.2
Subunit structure	Interacts with snoRNA U3. Interacts with MPP10. Component of the ribosomal small subunit (SSU) processome composed of at least 40 protein subunits and snoRNA U3. Ref.2
Subcellular location	Nucleus › nucleolus Ref.2.
Miscellaneous	Present with 9620 molecules/cell in log phase SD medium. Ref.3
Sequence similarities	Belongs to the WD repeat WDR3/UTP12 family. Contains 10 WD repeats.

Ontologies

Keywords
Biological process	Ribosome biogenesis rRNA processing
Cellular component	Nucleus
Domain	Repeat WD repeat
Molecular function	Ribonucleoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Inferred from mutant phenotype. Source: SGD endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Inferred from mutant phenotype. Source: SGD endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Inferred from mutant phenotype. Source: SGD
Cellular component	Pwp2p-containing subcomplex of 90S preribosome Inferred from direct assay. Source: SGD small-subunit processome Ref.2 Inferred from direct assay. Source: SGD
Molecular function	protein binding Inferred from physical interaction. Source: IntAct snoRNA binding Ref.2 Inferred from physical interaction. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
PWP2	P25635	1	EBI-5896,EBI-14332
UBP10	P53874	1	EBI-5896,EBI-19873

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 943	943	U3 small nucleolar RNA-associated protein 12		PRO_0000050955
Regions
	Repeat	77 – 107	31	WD 1
	Repeat	119 – 149	31	WD 2
	Repeat	161 – 190	30	WD 3
	Repeat	202 – 230	29	WD 4
	Repeat	389 – 418	30	WD 5
	Repeat	428 – 458	31	WD 6
	Repeat	471 – 501	31	WD 7
	Repeat	571 – 601	31	WD 8
	Repeat	613 – 643	31	WD 9
	Repeat	655 – 685	31	WD 10

Sequences

	Sequence		Length	Mass (Da)
	Q12220-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 7D9AEC7AF6A9C740	FASTA	943	106,342
	10 20 30 40 50 60 MVKSYQRFEQ AAAFGVIASN ANCVWIPASS GNSNGSGPGQ LITSALEDVN IWDIKTGDLV 70 80 90 100 110 120 SKLSDGLPPG ASDARGAKPA ECTYLEAHKD TDLLAVGYAD GVIKVWDLMS KTVLLNFNGH 130 140 150 160 170 180 KAAITLLQFD GTGTRLISGS KDSNIIVWDL VGEVGLYKLR SHKDSITGFW CQGEDWLIST 190 200 210 220 230 240 SKDGMIKLWD LKTHQCIETH IAHTGECWGL AVKDDLLITT GTDSQVKIWK LDIENDKMGG 250 260 270 280 290 300 KLTEMGIFEK QSKQRGLKIE FITNSSDKTS FFYIQNADKT IETFRIRKEE EIARGLKKRE 310 320 330 340 350 360 KRLKEKGLTE EEIAKSIKES YSSFILHPFQ TIRSLYKIKS ASWTTVSSSK LELVLTTSSN 370 380 390 400 410 420 TIEYYSIPYE KRDPTSPAPL KTHTIELQGQ RTDVRSIDIS DDNKLLATAS NGSLKIWNIK 430 440 450 460 470 480 THKCIRTFEC GYALTCKFLP GGLLVILGTR NGELQLFDLA SSSLLDTIED AHDAAIWSLD 490 500 510 520 530 540 LTSDGKRLVT GSADKTVKFW DFKVENSLVP GTKNKFLPVL KLHHDTTLEL TDDILCVRVS 550 560 570 580 590 600 PDDRYLAISL LDNTVKVFFL DSMKFYLSLY GHKLPVLSID ISFDSKMIIT SSADKNIKIW 610 620 630 640 650 660 GLDFGDCHKS LFAHQDSIMN VKFLPQSHNF FSCSKDAVVK YWDGEKFECI QKLYAHQSEV 670 680 690 700 710 720 WALAVATDGG FVVSSSHDHS IRIWEETEDQ VFLEEEKEKE LEEQYEDTLL TSLEEGNGDD 730 740 750 760 770 780 AFKADASGEG VEDEASGVHK QTLESLKAGE RLMEALDLGI AEIEGLEAYN RDMKLWQRKK 790 800 810 820 830 840 LGEAPIKPQG NAVLIAVNKT PEQYIMDTLL RIRMSQLEDA LMVMPFSYVL KFLKFIDTVM 850 860 870 880 890 900 QNKTLLHSHL PLICKNLFFI IKFNHKELVS QKNEELKLQI NRVKTELRSA LKSTEDDLGF 910 920 930 940 NVQGLKFVKQ QWNLRHNYEF VDEYDQQEKE SNSARKRVFG TVI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XII." Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H. , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D. Nature 387:87-90(1997) [PubMed: 9169871] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	"A large nucleolar U3 ribonucleoprotein required for 18S ribosomal RNA biogenesis." Dragon F., Gallagher J.E.G., Compagnone-Post P.A., Mitchell B.M., Porwancher K.A., Wehner K.A., Wormsley S., Settlage R.E., Shabanowitz J., Osheim Y., Beyer A.L., Hunt D.F., Baserga S.J. Nature 417:967-970(2002) [PubMed: 12068309] [Abstract] Cited for: FUNCTION, INTERACTION WITH MPP10 AND SNORNA U3, IDENTIFICATION IN SSU PROCESSOME BY MASS SPECTROMETRY, SUBCELLULAR LOCATION.
[3]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U53877 Genomic DNA. Translation: AAB82375.1. U53881 Genomic DNA. Translation: AAB82402.1. X91258 Genomic DNA. Translation: CAA62640.1. Z73301 Genomic DNA. Translation: CAA97699.1. Z73302 Genomic DNA. Translation: CAA97700.1. X89514 Genomic DNA. Translation: CAA61707.1.
PIR	S59317.
RefSeq	NP_013230.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-6494N.
IntAct	Q12220. 21 interactions.
STRING	Q12220.
Proteomic databases
PeptideAtlas	Q12220.
Genome annotation databases
Ensembl	YLR129W; YLR129W; YLR129W; Saccharomyces cerevisiae. [Genome view]
GeneID	850820.
KEGG	sce:YLR129W.
NMPDR	fig|4932.3.peg.4223.
Organism-specific databases
CYGD	YLR129w.
SGD	S000004119. DIP2.
Phylogenomic databases
HOGENOM	HBG379828.
OMA	SSADKNI.
OrthoDB	EOG9H73VF.
Gene expression databases
ArrayExpress	Q12220.
Genevestigator	Q12220.
GermOnline	YLR129W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR020472. G-protein_beta_WD-40_rep_reg. IPR007148. SSU_processome_Utp12. IPR015943. WD40/YVTN_repeat-like_dom. IPR001680. WD40_repeat. IPR011046. WD40_repeat-like_dom. IPR019782. WD40_repeat_2. IPR019775. WD40_repeat_CS. IPR017986. WD40_repeat_dom. IPR019781. WD40_repeat_sg. [Graphical view]
Gene3D	G3DSA:2.130.10.10. WD40/YVTN_repeat-like. 2 hits.
Pfam	PF04003. Utp12. 1 hit. PF00400. WD40. 9 hits. [Graphical view]
PRINTS	PR00320. GPROTEINBRPT.
SMART	SM00320. WD40. 11 hits. [Graphical view]
PROSITE	PS00678. WD_REPEATS_1. 4 hits. PS50082. WD_REPEATS_2. 8 hits. PS50294. WD_REPEATS_REGION. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	967067.

Entry information

Entry name

UTP12_YEAST

Accession

Primary (citable) accession number: Q12220
Secondary accession number(s): Q05386

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	November 1, 1996
Last modified:	December 15, 2009
This is version 82 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)