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Protein

Cell wall protein TIR4

Gene

TIR4

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Component of the cell wall. Required for anaerobic growth.1 Publication

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Cell wall biogenesis/degradation

Enzyme and pathway databases

BioCyciYEAST:G3O-33559-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Cell wall protein TIR4
Alternative name(s):
TIP1-related protein 4
Gene namesi
Name:TIR4
Ordered Locus Names:YOR009W
ORF Names:UNB487
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XV

Organism-specific databases

EuPathDBiFungiDB:YOR009W.
SGDiS000005535. TIR4.

Subcellular locationi

GO - Cellular componenti

  • anchored component of membrane Source: UniProtKB-KW
  • extracellular region Source: UniProtKB-KW
  • fungal-type cell wall Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cell wall, Membrane, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 22Sequence analysisAdd BLAST22
ChainiPRO_000026764023 – 465Cell wall protein TIR4Add BLAST443
PropeptideiPRO_0000267641466 – 487Removed in mature formSequence analysisAdd BLAST22

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi327N-linked (GlcNAc...)Sequence analysis1
Glycosylationi348N-linked (GlcNAc...)Sequence analysis1
Glycosylationi368N-linked (GlcNAc...)Sequence analysis1
Glycosylationi403N-linked (GlcNAc...)Sequence analysis1
Glycosylationi404N-linked (GlcNAc...)Sequence analysis1
Lipidationi465GPI-anchor amidated asparagineSequence analysis1

Post-translational modificationi

The GPI-anchor is attached to the protein in the endoplasmic reticulum and serves to target the protein to the cell surface. There, the glucosamine-inositol phospholipid moiety is cleaved off and the GPI-modified mannoprotein is covalently attached via its lipidless GPI glycan remnant to the 1,6-beta-glucan of the outer cell wall layer (By similarity).By similarity

Keywords - PTMi

Glycoprotein, GPI-anchor, Lipoprotein

Proteomic databases

TopDownProteomicsiQ12218.

Expressioni

Inductioni

Induced during anaerobic growth. Induced by cold shock.1 Publication

Interactioni

Protein-protein interaction databases

BioGridi34414. 28 interactors.
DIPiDIP-5194N.
IntActiQ12218. 1 interactor.
MINTiMINT-557897.

Structurei

3D structure databases

ProteinModelPortaliQ12218.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati137 – 14811 PublicationAdd BLAST12
Repeati149 – 16021 PublicationAdd BLAST12
Repeati161 – 17231 PublicationAdd BLAST12
Repeati173 – 18441 PublicationAdd BLAST12
Repeati185 – 19651 PublicationAdd BLAST12
Repeati197 – 20861 PublicationAdd BLAST12
Repeati209 – 22071 PublicationAdd BLAST12
Repeati221 – 23281 PublicationAdd BLAST12
Repeati233 – 24491 PublicationAdd BLAST12
Repeati245 – 256101 PublicationAdd BLAST12
Repeati257 – 268111 PublicationAdd BLAST12

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni137 – 26811 X 12 AA approximate tandem repeats, Ser-richAdd BLAST132

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi113 – 365Ser-richAdd BLAST253

Domaini

The number of the intragenic tandem repeats varies between different S.cerevisiae strains.

Sequence similaritiesi

Belongs to the SRP1/TIP1 family.Curated

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

GeneTreeiENSGT00700000104882.
InParanoidiQ12218.
OMAiDCPARTE.
OrthoDBiEOG092C5NOA.

Family and domain databases

InterProiIPR000992. SRP1_TIP1.
[Graphical view]
PfamiPF00660. SRP1_TIP1. 1 hit.
[Graphical view]
PROSITEiPS00724. SRP1_TIP1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q12218-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAYSKITLLA ALAAIAYAQT QAQINELNVV LDDVKTNIAD YITLSYTPNS
60 70 80 90 100
GFSLDQMPAG IMDIAAQLVA NPSDDSYTTL YSEVDFSAVE HMLTMVPWYS
110 120 130 140 150
SRLLPELEAM DASLTTSSSA ATSSSEVASS SIASSTSSSV APSSSEVVSS
160 170 180 190 200
SVAPSSSEVV SSSVAPSSSE VVSSSVASSS SEVASSSVAP SSSEVVSSSV
210 220 230 240 250
ASSSSEVASS SVAPSSSEVV SSSVAPSSSE VVSSSVASSS SEVASSSVAP
260 270 280 290 300
SSSEVVSSSV ASSTSEATSS SAVTSSSAVS SSTESVSSSS VSSSSAVSSS
310 320 330 340 350
EAVSSSPVSS VVSSSAGPAS SSVAPYNSTI ASSSSTAQTS ISTIAPYNST
360 370 380 390 400
TTTTPASSAS SVIISTRNGT TVTETDNTLV TKETTVCDYS STSAVPASTT
410 420 430 440 450
GYNNSTKVST ATICSTCKEG TSTATDFSTL KTTVTVCDSA CQAKKSATVV
460 470 480
SVQSKTTGIV EQTENGAAKA VIGMGAGALA AVAAMLL
Length:487
Mass (Da):47,849
Last modified:November 1, 1996 - v1
Checksum:iBA12415C400FAD65
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U43491 Genomic DNA. Translation: AAC49489.1.
Z74917 Genomic DNA. Translation: CAA99197.1.
BK006948 Genomic DNA. Translation: DAA10792.1.
PIRiS61993.
RefSeqiNP_014652.1. NM_001183428.1.

Genome annotation databases

EnsemblFungiiYOR009W; YOR009W; YOR009W.
GeneIDi854173.
KEGGisce:YOR009W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U43491 Genomic DNA. Translation: AAC49489.1.
Z74917 Genomic DNA. Translation: CAA99197.1.
BK006948 Genomic DNA. Translation: DAA10792.1.
PIRiS61993.
RefSeqiNP_014652.1. NM_001183428.1.

3D structure databases

ProteinModelPortaliQ12218.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34414. 28 interactors.
DIPiDIP-5194N.
IntActiQ12218. 1 interactor.
MINTiMINT-557897.

Proteomic databases

TopDownProteomicsiQ12218.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYOR009W; YOR009W; YOR009W.
GeneIDi854173.
KEGGisce:YOR009W.

Organism-specific databases

EuPathDBiFungiDB:YOR009W.
SGDiS000005535. TIR4.

Phylogenomic databases

GeneTreeiENSGT00700000104882.
InParanoidiQ12218.
OMAiDCPARTE.
OrthoDBiEOG092C5NOA.

Enzyme and pathway databases

BioCyciYEAST:G3O-33559-MONOMER.

Miscellaneous databases

PROiQ12218.

Family and domain databases

InterProiIPR000992. SRP1_TIP1.
[Graphical view]
PfamiPF00660. SRP1_TIP1. 1 hit.
[Graphical view]
PROSITEiPS00724. SRP1_TIP1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTIR4_YEAST
AccessioniPrimary (citable) accession number: Q12218
Secondary accession number(s): D6W276
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 12, 2006
Last sequence update: November 1, 1996
Last modified: November 2, 2016
This is version 107 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XV
    Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.