Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q12216

- SIZ2_YEAST

UniProt

Q12216 - SIZ2_YEAST

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

E3 SUMO-protein ligase SIZ2

Gene

NFI1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

May act as an E3 ligase mediating SUMO/Smt3 attachment to septins. May be involved in chromosome maintenance.2 Publications

Pathwayi

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri323 – 40078SP-RING-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. double-stranded DNA binding Source: SGD
  2. SUMO ligase activity Source: SGD
  3. zinc ion binding Source: InterPro

GO - Biological processi

  1. chromosome segregation Source: SGD
  2. negative regulation of gene silencing Source: SGD
  3. protein sumoylation Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Ubl conjugation pathway

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciYEAST:G3O-33673-MONOMER.
UniPathwayiUPA00886.

Names & Taxonomyi

Protein namesi
Recommended name:
E3 SUMO-protein ligase SIZ2 (EC:6.3.2.-)
Alternative name(s):
SAP and Miz-finger domain-containing protein 2
Gene namesi
Name:NFI1
Synonyms:SIZ2
Ordered Locus Names:YOR156C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XV

Organism-specific databases

CYGDiYOR156c.
SGDiS000005682. NFI1.

Subcellular locationi

Nucleus 1 Publication

GO - Cellular componenti

  1. nuclear chromatin Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 726726E3 SUMO-protein ligase SIZ2PRO_0000218985Add
BLAST

Post-translational modificationi

Autosumoylated upon ethanol stress.1 Publication

Keywords - PTMi

Ubl conjugation

Proteomic databases

PaxDbiQ12216.

Expressioni

Gene expression databases

GenevestigatoriQ12216.

Interactioni

Subunit structurei

Interacts with CDC12.

Protein-protein interaction databases

BioGridi34552. 150 interactions.
DIPiDIP-5775N.
IntActiQ12216. 12 interactions.
MINTiMINT-582835.
STRINGi4932.YOR156C.

Structurei

3D structure databases

ProteinModelPortaliQ12216.
SMRiQ12216. Positions 32-120, 156-420.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini43 – 7735SAPPROSITE-ProRule annotationAdd
BLAST
Domaini139 – 291153PINITPROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi7 – 1610Poly-Asn

Sequence similaritiesi

Belongs to the PIAS family.Curated
Contains 1 PINIT domain.PROSITE-ProRule annotation
Contains 1 SAP domain.PROSITE-ProRule annotation
Contains 1 SP-RING-type zinc finger.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri323 – 40078SP-RING-typePROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiNOG125513.
GeneTreeiENSGT00550000074410.
InParanoidiQ12216.
OrthoDBiEOG7HHX2J.

Family and domain databases

Gene3Di1.10.720.30. 1 hit.
3.30.40.10. 1 hit.
InterProiIPR023321. PINIT.
IPR003034. SAP_dom.
IPR027229. SIZ1/SIZ2/Pli1/Gei17.
IPR004181. Znf_MIZ.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PANTHERiPTHR10782:SF4. PTHR10782:SF4. 1 hit.
PfamiPF14324. PINIT. 1 hit.
PF02037. SAP. 1 hit.
PF02891. zf-MIZ. 1 hit.
[Graphical view]
SMARTiSM00513. SAP. 1 hit.
[Graphical view]
PROSITEiPS51466. PINIT. 1 hit.
PS50800. SAP. 1 hit.
PS51044. ZF_SP_RING. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q12216-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MASVMSNNNN NNNNNNASYM FTNPLSNTGG GLINEIKDAI NEMEQLKVLE
60 70 80 90 100
LKQICKSLDL SITGKKAVLQ DRIKQFLRKS CDIGHIDPWR PKAIKILIAK
110 120 130 140 150
VRINSSLPKY STLWETLKTG AFKHPVASGQ LPVTALQSTA LPPYSQQQAL
160 170 180 190 200
AYSFTSPFYK PIVQIPDANK KLKQSAGRGC TKMKFKVSKS NHDLLKSNKS
210 220 230 240 250
YKLYLFSGFS IPFIYETVGH EAIDFPYPCE LVFNGTKLED NVKGLKKQNG
260 270 280 290 300
TGNPANLTPY LKVPTEMNHL DLHYLNIDKE YSISCFIVEV FSPEALLGKI
310 320 330 340 350
LKRPKIIKQA TTAYIKRTLN EQDDDDIITT STVLSLQCPI SCTRMKYPAK
360 370 380 390 400
TDQCKHIQCF DALWFLHSQS QVPTWQCPIC QHPIKFDQLK ISEFVDNIIQ
410 420 430 440 450
NCNEDVEQVE ISVDGSWKPI HNSSAVITDT VNQNHSVKNE NQGTVKQEQD
460 470 480 490 500
YDSRNAFDTN LRNGSNHNEP EIISLDSSDD EAFIPASKSF PTHVNPRNDQ
510 520 530 540 550
LRADIFPSES EGSSDYNPNH TSTPKGSPTM DQDNYQDAFQ MRSFLNQGAT
560 570 580 590 600
TNINDTPTNN SSINSFVTAT NGDSRIFYNR GPSTPLLPAV LQNLTNQTEA
610 620 630 640 650
QRNPYGPNYN TTAQDRNLLG IEGDLPPIPP VDPNSEAETE LPTRTTSAAH
660 670 680 690 700
LPPYIHVSTS GHGDDGKIRK RRHSNVSIYI PKNPYATLMK RRPQANHAIM
710 720
NKTLAQTNDF NTSAQDNSEV VDLTSD
Length:726
Mass (Da):81,178
Last modified:November 1, 1996 - v1
Checksum:i74F0F839C5E74BAB
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U33152 Genomic DNA. Translation: AAA86121.1.
U55020 Genomic DNA. Translation: AAC49642.1.
Z75064 Genomic DNA. Translation: CAA99362.1.
BK006948 Genomic DNA. Translation: DAA10929.1.
PIRiS67044.
RefSeqiNP_014799.1. NM_001183575.1.

Genome annotation databases

EnsemblFungiiYOR156C; YOR156C; YOR156C.
GeneIDi854327.
KEGGisce:YOR156C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U33152 Genomic DNA. Translation: AAA86121.1 .
U55020 Genomic DNA. Translation: AAC49642.1 .
Z75064 Genomic DNA. Translation: CAA99362.1 .
BK006948 Genomic DNA. Translation: DAA10929.1 .
PIRi S67044.
RefSeqi NP_014799.1. NM_001183575.1.

3D structure databases

ProteinModelPortali Q12216.
SMRi Q12216. Positions 32-120, 156-420.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 34552. 150 interactions.
DIPi DIP-5775N.
IntActi Q12216. 12 interactions.
MINTi MINT-582835.
STRINGi 4932.YOR156C.

Proteomic databases

PaxDbi Q12216.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YOR156C ; YOR156C ; YOR156C .
GeneIDi 854327.
KEGGi sce:YOR156C.

Organism-specific databases

CYGDi YOR156c.
SGDi S000005682. NFI1.

Phylogenomic databases

eggNOGi NOG125513.
GeneTreei ENSGT00550000074410.
InParanoidi Q12216.
OrthoDBi EOG7HHX2J.

Enzyme and pathway databases

UniPathwayi UPA00886 .
BioCyci YEAST:G3O-33673-MONOMER.

Miscellaneous databases

NextBioi 976375.

Gene expression databases

Genevestigatori Q12216.

Family and domain databases

Gene3Di 1.10.720.30. 1 hit.
3.30.40.10. 1 hit.
InterProi IPR023321. PINIT.
IPR003034. SAP_dom.
IPR027229. SIZ1/SIZ2/Pli1/Gei17.
IPR004181. Znf_MIZ.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view ]
PANTHERi PTHR10782:SF4. PTHR10782:SF4. 1 hit.
Pfami PF14324. PINIT. 1 hit.
PF02037. SAP. 1 hit.
PF02891. zf-MIZ. 1 hit.
[Graphical view ]
SMARTi SM00513. SAP. 1 hit.
[Graphical view ]
PROSITEi PS51466. PINIT. 1 hit.
PS50800. SAP. 1 hit.
PS51044. ZF_SP_RING. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "S. cerevisiae two-hybrid interactor with the C-terminus of Cdc12p."
    De Virgilio C., Pringle J.R.
    Submitted (SEP-1995) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. "Analysis of a 35.6 kb region on the right arm of Saccharomyces cerevisiae chromosome XV."
    Bordonne R., Camasses A., Madania A., Poch O., Tarassov I.A., Winsor B., Martin R.P.
    Yeast 13:73-83(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: S288c / FY1678.
  3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XV."
    Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D.
    , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.
    Nature 387:98-102(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. "A novel factor required for the SUMO1/Smt3 conjugation of yeast septins."
    Takahashi Y., Toh-e A., Kikuchi Y.
    Gene 275:223-231(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
  6. "Saccharomyces cerevisiae SMT4 encodes an evolutionarily conserved protease with a role in chromosome condensation regulation."
    Strunnikov A.V., Aravind L., Koonin E.V.
    Genetics 158:95-107(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  7. "Comparative analysis of yeast PIAS-type SUMO ligases in vivo and in vitro."
    Takahashi Y., Toh-e A., Kikuchi Y.
    J. Biochem. 133:415-422(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  8. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  9. "Global analyses of sumoylated proteins in Saccharomyces cerevisiae. Induction of protein sumoylation by cellular stresses."
    Zhou W., Ryan J.J., Zhou H.
    J. Biol. Chem. 279:32262-32268(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUMOYLATION [LARGE SCALE ANALYSIS].
  10. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiSIZ2_YEAST
AccessioniPrimary (citable) accession number: Q12216
Secondary accession number(s): D6W2L3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: October 29, 2014
This is version 107 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 3170 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XV
    Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

External Data

Dasty 3