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Protein

E3 SUMO-protein ligase SIZ2

Gene

NFI1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

May act as an E3 ligase mediating SUMO/Smt3 attachment to septins. May be involved in chromosome maintenance.2 Publications

Miscellaneous

Present with 3170 molecules/cell in log phase SD medium.1 Publication

Pathwayi: protein sumoylation

This protein is involved in the pathway protein sumoylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein sumoylation and in Protein modification.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri323 – 400SP-RING-typePROSITE-ProRule annotationAdd BLAST78

GO - Molecular functioni

  • double-stranded DNA binding Source: SGD
  • SUMO transferase activity Source: SGD
  • zinc ion binding Source: InterPro

GO - Biological processi

  • chromosome segregation Source: SGD
  • DNA double-strand break attachment to nuclear envelope Source: SGD
  • negative regulation of gene silencing Source: SGD
  • protein sumoylation Source: SGD

Keywordsi

Molecular functionTransferase
Biological processUbl conjugation pathway
LigandMetal-binding, Zinc

Enzyme and pathway databases

BioCyciYEAST:G3O-33673-MONOMER
ReactomeiR-SCE-3108214 SUMOylation of DNA damage response and repair proteins
R-SCE-4615885 SUMOylation of DNA replication proteins
R-SCE-5693565 Recruitment and ATM-mediated phosphorylation of repair and signaling proteins at DNA double strand breaks
R-SCE-5696395 Formation of Incision Complex in GG-NER
UniPathwayiUPA00886

Names & Taxonomyi

Protein namesi
Recommended name:
E3 SUMO-protein ligase SIZ2 (EC:2.3.2.-)
Alternative name(s):
E3 SUMO-protein transferase SIZ2Curated
SAP and Miz-finger domain-containing protein 2
Gene namesi
Name:NFI1
Synonyms:SIZ2
Ordered Locus Names:YOR156C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XV

Organism-specific databases

EuPathDBiFungiDB:YOR156C
SGDiS000005682 NFI1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002189851 – 726E3 SUMO-protein ligase SIZ2Add BLAST726

Post-translational modificationi

Autosumoylated upon ethanol stress.1 Publication

Keywords - PTMi

Ubl conjugation

Proteomic databases

PaxDbiQ12216
PRIDEiQ12216

PTM databases

iPTMnetiQ12216

Interactioni

Subunit structurei

Interacts with CDC12.

Protein-protein interaction databases

BioGridi34552, 210 interactors
DIPiDIP-5775N
IntActiQ12216, 13 interactors
MINTiQ12216
STRINGi4932.YOR156C

Structurei

3D structure databases

ProteinModelPortaliQ12216
SMRiQ12216
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini43 – 77SAPPROSITE-ProRule annotationAdd BLAST35
Domaini139 – 291PINITPROSITE-ProRule annotationAdd BLAST153

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi7 – 16Poly-Asn10

Sequence similaritiesi

Belongs to the PIAS family.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri323 – 400SP-RING-typePROSITE-ProRule annotationAdd BLAST78

Keywords - Domaini

Zinc-finger

Phylogenomic databases

GeneTreeiENSGT00550000074410
InParanoidiQ12216
OrthoDBiEOG092C445R

Family and domain databases

Gene3Di1.10.720.30, 1 hit
2.60.120.780, 1 hit
3.30.40.10, 1 hit
InterProiView protein in InterPro
IPR023321 PINIT
IPR038654 PINIT_sf
IPR003034 SAP_dom
IPR036361 SAP_dom_sf
IPR027229 SIZ1/SIZ2/Pli1/Gei17
IPR004181 Znf_MIZ
IPR013083 Znf_RING/FYVE/PHD
PANTHERiPTHR10782:SF4 PTHR10782:SF4, 1 hit
PfamiView protein in Pfam
PF14324 PINIT, 1 hit
PF02037 SAP, 1 hit
PF02891 zf-MIZ, 1 hit
SMARTiView protein in SMART
SM00513 SAP, 1 hit
SUPFAMiSSF68906 SSF68906, 1 hit
PROSITEiView protein in PROSITE
PS51466 PINIT, 1 hit
PS50800 SAP, 1 hit
PS51044 ZF_SP_RING, 1 hit

Sequencei

Sequence statusi: Complete.

Q12216-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASVMSNNNN NNNNNNASYM FTNPLSNTGG GLINEIKDAI NEMEQLKVLE
60 70 80 90 100
LKQICKSLDL SITGKKAVLQ DRIKQFLRKS CDIGHIDPWR PKAIKILIAK
110 120 130 140 150
VRINSSLPKY STLWETLKTG AFKHPVASGQ LPVTALQSTA LPPYSQQQAL
160 170 180 190 200
AYSFTSPFYK PIVQIPDANK KLKQSAGRGC TKMKFKVSKS NHDLLKSNKS
210 220 230 240 250
YKLYLFSGFS IPFIYETVGH EAIDFPYPCE LVFNGTKLED NVKGLKKQNG
260 270 280 290 300
TGNPANLTPY LKVPTEMNHL DLHYLNIDKE YSISCFIVEV FSPEALLGKI
310 320 330 340 350
LKRPKIIKQA TTAYIKRTLN EQDDDDIITT STVLSLQCPI SCTRMKYPAK
360 370 380 390 400
TDQCKHIQCF DALWFLHSQS QVPTWQCPIC QHPIKFDQLK ISEFVDNIIQ
410 420 430 440 450
NCNEDVEQVE ISVDGSWKPI HNSSAVITDT VNQNHSVKNE NQGTVKQEQD
460 470 480 490 500
YDSRNAFDTN LRNGSNHNEP EIISLDSSDD EAFIPASKSF PTHVNPRNDQ
510 520 530 540 550
LRADIFPSES EGSSDYNPNH TSTPKGSPTM DQDNYQDAFQ MRSFLNQGAT
560 570 580 590 600
TNINDTPTNN SSINSFVTAT NGDSRIFYNR GPSTPLLPAV LQNLTNQTEA
610 620 630 640 650
QRNPYGPNYN TTAQDRNLLG IEGDLPPIPP VDPNSEAETE LPTRTTSAAH
660 670 680 690 700
LPPYIHVSTS GHGDDGKIRK RRHSNVSIYI PKNPYATLMK RRPQANHAIM
710 720
NKTLAQTNDF NTSAQDNSEV VDLTSD
Length:726
Mass (Da):81,178
Last modified:November 1, 1996 - v1
Checksum:i74F0F839C5E74BAB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U33152 Genomic DNA Translation: AAA86121.1
U55020 Genomic DNA Translation: AAC49642.1
Z75064 Genomic DNA Translation: CAA99362.1
BK006948 Genomic DNA Translation: DAA10929.1
PIRiS67044
RefSeqiNP_014799.1, NM_001183575.1

Genome annotation databases

EnsemblFungiiYOR156C; YOR156C; YOR156C
GeneIDi854327
KEGGisce:YOR156C

Similar proteinsi

Entry informationi

Entry nameiSIZ2_YEAST
AccessioniPrimary (citable) accession number: Q12216
Secondary accession number(s): D6W2L3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: May 23, 2018
This is version 138 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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