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Q12216

- SIZ2_YEAST

UniProt

Q12216 - SIZ2_YEAST

Protein

E3 SUMO-protein ligase SIZ2

Gene

NFI1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 106 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    May act as an E3 ligase mediating SUMO/Smt3 attachment to septins. May be involved in chromosome maintenance.2 Publications

    Pathwayi

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri323 – 40078SP-RING-typePROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. double-stranded DNA binding Source: SGD
    2. SUMO ligase activity Source: SGD
    3. zinc ion binding Source: InterPro

    GO - Biological processi

    1. chromosome condensation Source: SGD
    2. chromosome segregation Source: SGD
    3. negative regulation of gene silencing Source: SGD
    4. protein sumoylation Source: SGD

    Keywords - Molecular functioni

    Ligase

    Keywords - Biological processi

    Ubl conjugation pathway

    Keywords - Ligandi

    Metal-binding, Zinc

    Enzyme and pathway databases

    BioCyciYEAST:G3O-33673-MONOMER.
    UniPathwayiUPA00886.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    E3 SUMO-protein ligase SIZ2 (EC:6.3.2.-)
    Alternative name(s):
    SAP and Miz-finger domain-containing protein 2
    Gene namesi
    Name:NFI1
    Synonyms:SIZ2
    Ordered Locus Names:YOR156C
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XV

    Organism-specific databases

    CYGDiYOR156c.
    SGDiS000005682. NFI1.

    Subcellular locationi

    Nucleus 1 Publication

    GO - Cellular componenti

    1. nuclear chromatin Source: SGD

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 726726E3 SUMO-protein ligase SIZ2PRO_0000218985Add
    BLAST

    Post-translational modificationi

    Autosumoylated upon ethanol stress.1 Publication

    Keywords - PTMi

    Ubl conjugation

    Proteomic databases

    PaxDbiQ12216.

    Expressioni

    Gene expression databases

    GenevestigatoriQ12216.

    Interactioni

    Subunit structurei

    Interacts with CDC12.

    Protein-protein interaction databases

    BioGridi34552. 149 interactions.
    DIPiDIP-5775N.
    IntActiQ12216. 12 interactions.
    MINTiMINT-582835.
    STRINGi4932.YOR156C.

    Structurei

    3D structure databases

    ProteinModelPortaliQ12216.
    SMRiQ12216. Positions 32-120, 156-420.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini43 – 7735SAPPROSITE-ProRule annotationAdd
    BLAST
    Domaini139 – 291153PINITPROSITE-ProRule annotationAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi7 – 1610Poly-Asn

    Sequence similaritiesi

    Belongs to the PIAS family.Curated
    Contains 1 PINIT domain.PROSITE-ProRule annotation
    Contains 1 SAP domain.PROSITE-ProRule annotation
    Contains 1 SP-RING-type zinc finger.PROSITE-ProRule annotation

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri323 – 40078SP-RING-typePROSITE-ProRule annotationAdd
    BLAST

    Keywords - Domaini

    Zinc-finger

    Phylogenomic databases

    eggNOGiNOG125513.
    GeneTreeiENSGT00550000074410.
    OrthoDBiEOG7HHX2J.

    Family and domain databases

    Gene3Di1.10.720.30. 1 hit.
    3.30.40.10. 1 hit.
    InterProiIPR023321. PINIT.
    IPR003034. SAP_dom.
    IPR027229. SIZ1/SIZ2/Pli1/Gei17.
    IPR004181. Znf_MIZ.
    IPR013083. Znf_RING/FYVE/PHD.
    [Graphical view]
    PANTHERiPTHR10782:SF4. PTHR10782:SF4. 1 hit.
    PfamiPF14324. PINIT. 1 hit.
    PF02037. SAP. 1 hit.
    PF02891. zf-MIZ. 1 hit.
    [Graphical view]
    SMARTiSM00513. SAP. 1 hit.
    [Graphical view]
    PROSITEiPS51466. PINIT. 1 hit.
    PS50800. SAP. 1 hit.
    PS51044. ZF_SP_RING. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q12216-1 [UniParc]FASTAAdd to Basket

    « Hide

    MASVMSNNNN NNNNNNASYM FTNPLSNTGG GLINEIKDAI NEMEQLKVLE    50
    LKQICKSLDL SITGKKAVLQ DRIKQFLRKS CDIGHIDPWR PKAIKILIAK 100
    VRINSSLPKY STLWETLKTG AFKHPVASGQ LPVTALQSTA LPPYSQQQAL 150
    AYSFTSPFYK PIVQIPDANK KLKQSAGRGC TKMKFKVSKS NHDLLKSNKS 200
    YKLYLFSGFS IPFIYETVGH EAIDFPYPCE LVFNGTKLED NVKGLKKQNG 250
    TGNPANLTPY LKVPTEMNHL DLHYLNIDKE YSISCFIVEV FSPEALLGKI 300
    LKRPKIIKQA TTAYIKRTLN EQDDDDIITT STVLSLQCPI SCTRMKYPAK 350
    TDQCKHIQCF DALWFLHSQS QVPTWQCPIC QHPIKFDQLK ISEFVDNIIQ 400
    NCNEDVEQVE ISVDGSWKPI HNSSAVITDT VNQNHSVKNE NQGTVKQEQD 450
    YDSRNAFDTN LRNGSNHNEP EIISLDSSDD EAFIPASKSF PTHVNPRNDQ 500
    LRADIFPSES EGSSDYNPNH TSTPKGSPTM DQDNYQDAFQ MRSFLNQGAT 550
    TNINDTPTNN SSINSFVTAT NGDSRIFYNR GPSTPLLPAV LQNLTNQTEA 600
    QRNPYGPNYN TTAQDRNLLG IEGDLPPIPP VDPNSEAETE LPTRTTSAAH 650
    LPPYIHVSTS GHGDDGKIRK RRHSNVSIYI PKNPYATLMK RRPQANHAIM 700
    NKTLAQTNDF NTSAQDNSEV VDLTSD 726
    Length:726
    Mass (Da):81,178
    Last modified:November 1, 1996 - v1
    Checksum:i74F0F839C5E74BAB
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U33152 Genomic DNA. Translation: AAA86121.1.
    U55020 Genomic DNA. Translation: AAC49642.1.
    Z75064 Genomic DNA. Translation: CAA99362.1.
    BK006948 Genomic DNA. Translation: DAA10929.1.
    PIRiS67044.
    RefSeqiNP_014799.1. NM_001183575.1.

    Genome annotation databases

    EnsemblFungiiYOR156C; YOR156C; YOR156C.
    GeneIDi854327.
    KEGGisce:YOR156C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U33152 Genomic DNA. Translation: AAA86121.1 .
    U55020 Genomic DNA. Translation: AAC49642.1 .
    Z75064 Genomic DNA. Translation: CAA99362.1 .
    BK006948 Genomic DNA. Translation: DAA10929.1 .
    PIRi S67044.
    RefSeqi NP_014799.1. NM_001183575.1.

    3D structure databases

    ProteinModelPortali Q12216.
    SMRi Q12216. Positions 32-120, 156-420.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 34552. 149 interactions.
    DIPi DIP-5775N.
    IntActi Q12216. 12 interactions.
    MINTi MINT-582835.
    STRINGi 4932.YOR156C.

    Proteomic databases

    PaxDbi Q12216.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YOR156C ; YOR156C ; YOR156C .
    GeneIDi 854327.
    KEGGi sce:YOR156C.

    Organism-specific databases

    CYGDi YOR156c.
    SGDi S000005682. NFI1.

    Phylogenomic databases

    eggNOGi NOG125513.
    GeneTreei ENSGT00550000074410.
    OrthoDBi EOG7HHX2J.

    Enzyme and pathway databases

    UniPathwayi UPA00886 .
    BioCyci YEAST:G3O-33673-MONOMER.

    Miscellaneous databases

    NextBioi 976375.

    Gene expression databases

    Genevestigatori Q12216.

    Family and domain databases

    Gene3Di 1.10.720.30. 1 hit.
    3.30.40.10. 1 hit.
    InterProi IPR023321. PINIT.
    IPR003034. SAP_dom.
    IPR027229. SIZ1/SIZ2/Pli1/Gei17.
    IPR004181. Znf_MIZ.
    IPR013083. Znf_RING/FYVE/PHD.
    [Graphical view ]
    PANTHERi PTHR10782:SF4. PTHR10782:SF4. 1 hit.
    Pfami PF14324. PINIT. 1 hit.
    PF02037. SAP. 1 hit.
    PF02891. zf-MIZ. 1 hit.
    [Graphical view ]
    SMARTi SM00513. SAP. 1 hit.
    [Graphical view ]
    PROSITEi PS51466. PINIT. 1 hit.
    PS50800. SAP. 1 hit.
    PS51044. ZF_SP_RING. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "S. cerevisiae two-hybrid interactor with the C-terminus of Cdc12p."
      De Virgilio C., Pringle J.R.
      Submitted (SEP-1995) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. "Analysis of a 35.6 kb region on the right arm of Saccharomyces cerevisiae chromosome XV."
      Bordonne R., Camasses A., Madania A., Poch O., Tarassov I.A., Winsor B., Martin R.P.
      Yeast 13:73-83(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: S288c / FY1678.
    3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XV."
      Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D.
      , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.
      Nature 387:98-102(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    5. "A novel factor required for the SUMO1/Smt3 conjugation of yeast septins."
      Takahashi Y., Toh-e A., Kikuchi Y.
      Gene 275:223-231(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION.
    6. "Saccharomyces cerevisiae SMT4 encodes an evolutionarily conserved protease with a role in chromosome condensation regulation."
      Strunnikov A.V., Aravind L., Koonin E.V.
      Genetics 158:95-107(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    7. "Comparative analysis of yeast PIAS-type SUMO ligases in vivo and in vitro."
      Takahashi Y., Toh-e A., Kikuchi Y.
      J. Biochem. 133:415-422(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    8. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    9. "Global analyses of sumoylated proteins in Saccharomyces cerevisiae. Induction of protein sumoylation by cellular stresses."
      Zhou W., Ryan J.J., Zhou H.
      J. Biol. Chem. 279:32262-32268(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUMOYLATION [LARGE SCALE ANALYSIS].
    10. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiSIZ2_YEAST
    AccessioniPrimary (citable) accession number: Q12216
    Secondary accession number(s): D6W2L3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 106 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 3170 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome XV
      Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

    External Data

    Dasty 3