Q12216 (SIZ2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
E3 SUMO-protein ligase SIZ2
EC=6.3.2.-

Alternative name(s):
SAP and Miz-finger domain-containing protein 2

Gene names

Name:	NFI1
Synonyms:	SIZ2
Ordered Locus Names:	YOR156C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	726 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May act as an E3 ligase mediating SUMO/Smt3 attachment to septins. May be involved in chromosome maintenance. Ref.6 Ref.7
Pathway	Protein modification; protein sumoylation.
Subunit structure	Interacts with CDC12.
Subcellular location	Nucleus Ref.5.
Post-translational modification	Autosumoylated upon ethanol stress.
Miscellaneous	Present with 3170 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the PIAS family. Contains 1 PINIT domain. Contains 1 SAP domain. Contains 1 SP-RING-type zinc finger.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Cellular component	Nucleus
Domain	Zinc-finger
Ligand	Metal-binding Zinc
Molecular function	Ligase
PTM	Phosphoprotein Ubl conjugation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	chromosome condensation Traceable author statement PubMed 12402242. Source: SGD chromosome segregation Inferred from mutant phenotype PubMed 16204216. Source: SGD protein sumoylation Inferred from direct assay Ref.7. Source: SGD
Cellular_component	cytoplasm Inferred from direct assay PubMed 14562095. Source: SGD nuclear chromatin Inferred from direct assay PubMed 16204216. Source: SGD
Molecular_function	SUMO ligase activity Inferred from direct assay Ref.7. Source: SGD nucleic acid binding Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 726	726	E3 SUMO-protein ligase SIZ2		PRO_0000218985
Regions
	Domain	43 – 77	35	SAP
	Domain	139 – 291	153	PINIT
	Zinc finger	323 – 400	78	SP-RING-type
	Compositional bias	7 – 16	10	Poly-Asn
Amino acid modifications
	Modified residue	527	1	Phosphoserine Ref.10

Sequences

	Sequence		Length	Mass (Da)
	Q12216 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 74F0F839C5E74BAB	FASTA	726	81,178
	10 20 30 40 50 60 MASVMSNNNN NNNNNNASYM FTNPLSNTGG GLINEIKDAI NEMEQLKVLE LKQICKSLDL 70 80 90 100 110 120 SITGKKAVLQ DRIKQFLRKS CDIGHIDPWR PKAIKILIAK VRINSSLPKY STLWETLKTG 130 140 150 160 170 180 AFKHPVASGQ LPVTALQSTA LPPYSQQQAL AYSFTSPFYK PIVQIPDANK KLKQSAGRGC 190 200 210 220 230 240 TKMKFKVSKS NHDLLKSNKS YKLYLFSGFS IPFIYETVGH EAIDFPYPCE LVFNGTKLED 250 260 270 280 290 300 NVKGLKKQNG TGNPANLTPY LKVPTEMNHL DLHYLNIDKE YSISCFIVEV FSPEALLGKI 310 320 330 340 350 360 LKRPKIIKQA TTAYIKRTLN EQDDDDIITT STVLSLQCPI SCTRMKYPAK TDQCKHIQCF 370 380 390 400 410 420 DALWFLHSQS QVPTWQCPIC QHPIKFDQLK ISEFVDNIIQ NCNEDVEQVE ISVDGSWKPI 430 440 450 460 470 480 HNSSAVITDT VNQNHSVKNE NQGTVKQEQD YDSRNAFDTN LRNGSNHNEP EIISLDSSDD 490 500 510 520 530 540 EAFIPASKSF PTHVNPRNDQ LRADIFPSES EGSSDYNPNH TSTPKGSPTM DQDNYQDAFQ 550 560 570 580 590 600 MRSFLNQGAT TNINDTPTNN SSINSFVTAT NGDSRIFYNR GPSTPLLPAV LQNLTNQTEA 610 620 630 640 650 660 QRNPYGPNYN TTAQDRNLLG IEGDLPPIPP VDPNSEAETE LPTRTTSAAH LPPYIHVSTS 670 680 690 700 710 720 GHGDDGKIRK RRHSNVSIYI PKNPYATLMK RRPQANHAIM NKTLAQTNDF NTSAQDNSEV VDLTSD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Pringle J.R. Submitted (SEP-1995) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	"Analysis of a 35.6 kb region on the right arm of Saccharomyces cerevisiae chromosome XV." Bordonne R., Camasses A., Madania A., Poch O., Tarassov I.A., Winsor B., Martin R.P. Yeast 13:73-83(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: S288c / FY1678.
[3]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XV." Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D. , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K. Nature 387:98-102(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: S288c / FY1678.
[4]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[5]	"A novel factor required for the SUMO1/Smt3 conjugation of yeast septins." Takahashi Y., Toh-e A., Kikuchi Y. Gene 275:223-231(2001) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION.
[6]	"Saccharomyces cerevisiae SMT4 encodes an evolutionarily conserved protease with a role in chromosome condensation regulation." Strunnikov A.V., Aravind L., Koonin E.V. Genetics 158:95-107(2001) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[7]	"Comparative analysis of yeast PIAS-type SUMO ligases in vivo and in vitro." Takahashi Y., Toh-e A., Kikuchi Y. J. Biochem. 133:415-422(2003) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[8]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[9]	"Global analyses of sumoylated proteins in Saccharomyces cerevisiae. Induction of protein sumoylation by cellular stresses." Zhou W., Ryan J.J., Zhou H. J. Biol. Chem. 279:32262-32268(2004) [PubMed] [Europe PMC] [Abstract] Cited for: SUMOYLATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.
[10]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-527, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U33152 Genomic DNA. Translation: AAA86121.1. U55020 Genomic DNA. Translation: AAC49642.1. Z75064 Genomic DNA. Translation: CAA99362.1. BK006948 Genomic DNA. Translation: DAA10929.1.
PIR	S67044.
RefSeq	NP_014799.1. NM_001183575.1.
3D structure databases
ProteinModelPortal	Q12216.
SMR	Q12216. Positions 32-120, 156-420.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-5775N.
IntAct	Q12216. 12 interactions.
MINT	MINT-582835.
STRING	4932.YOR156C.
Proteomic databases
PaxDb	Q12216.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YOR156C; YOR156C; YOR156C.
GeneID	854327.
KEGG	sce:YOR156C.
Organism-specific databases
CYGD	YOR156c.
SGD	S000005682. NFI1.
Phylogenomic databases
eggNOG	NOG125513.
GeneTree	ENSGT00550000074410.
OrthoDB	EOG4004TM.
Enzyme and pathway databases
UniPathway	UPA00886.
Gene expression databases
Genevestigator	Q12216.
GermOnline	YOR156C. Saccharomyces cerevisiae.
Family and domain databases
Gene3D	1.10.720.30. 1 hit. 3.30.40.10. 1 hit.
InterPro	IPR023321. PINIT. IPR003034. SAP_dom. IPR027229. SIZ1/SIZ2/Pli1/Gei17. IPR004181. Znf_MIZ. IPR013083. Znf_RING/FYVE/PHD. [Graphical view]
PANTHER	PTHR10782:SF13. PTHR10782:SF13. 1 hit.
Pfam	PF14324. PINIT. 1 hit. PF02037. SAP. 1 hit. PF02891. zf-MIZ. 1 hit. [Graphical view]
SMART	SM00513. SAP. 1 hit. [Graphical view]
PROSITE	PS51466. PINIT. 1 hit. PS50800. SAP. 1 hit. PS51044. ZF_SP_RING. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	976375.

Entry information

Entry name

SIZ2_YEAST

Accession

Primary (citable) accession number: Q12216
Secondary accession number(s): D6W2L3

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1996
Last modified:	May 1, 2013
This is version 94 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program