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Protein

E3 SUMO-protein ligase SIZ2

Gene

NFI1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May act as an E3 ligase mediating SUMO/Smt3 attachment to septins. May be involved in chromosome maintenance.2 Publications

Pathwayi

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri323 – 40078SP-RING-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. double-stranded DNA binding Source: SGD
  2. ligase activity Source: UniProtKB-KW
  3. SUMO transferase activity Source: SGD
  4. zinc ion binding Source: InterPro

GO - Biological processi

  1. chromosome segregation Source: SGD
  2. negative regulation of gene silencing Source: SGD
  3. protein sumoylation Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Ubl conjugation pathway

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciYEAST:G3O-33673-MONOMER.
UniPathwayiUPA00886.

Names & Taxonomyi

Protein namesi
Recommended name:
E3 SUMO-protein ligase SIZ2 (EC:6.3.2.-)
Alternative name(s):
SAP and Miz-finger domain-containing protein 2
Gene namesi
Name:NFI1
Synonyms:SIZ2
Ordered Locus Names:YOR156C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311 Componenti: Chromosome XV

Organism-specific databases

CYGDiYOR156c.
EuPathDBiFungiDB:YOR156C.
SGDiS000005682. NFI1.

Subcellular locationi

  1. Nucleus 1 Publication

GO - Cellular componenti

  1. nuclear chromatin Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 726726E3 SUMO-protein ligase SIZ2PRO_0000218985Add
BLAST

Post-translational modificationi

Autosumoylated upon ethanol stress.1 Publication

Keywords - PTMi

Ubl conjugation

Proteomic databases

PaxDbiQ12216.

Expressioni

Gene expression databases

GenevestigatoriQ12216.

Interactioni

Subunit structurei

Interacts with CDC12.

Protein-protein interaction databases

BioGridi34552. 157 interactions.
DIPiDIP-5775N.
IntActiQ12216. 12 interactions.
MINTiMINT-582835.
STRINGi4932.YOR156C.

Structurei

3D structure databases

ProteinModelPortaliQ12216.
SMRiQ12216. Positions 32-120, 156-420.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini43 – 7735SAPPROSITE-ProRule annotationAdd
BLAST
Domaini139 – 291153PINITPROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi7 – 1610Poly-Asn

Sequence similaritiesi

Belongs to the PIAS family.Curated
Contains 1 PINIT domain.PROSITE-ProRule annotation
Contains 1 SAP domain.PROSITE-ProRule annotation
Contains 1 SP-RING-type zinc finger.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri323 – 40078SP-RING-typePROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiNOG125513.
GeneTreeiENSGT00550000074410.
InParanoidiQ12216.
OrthoDBiEOG7HHX2J.

Family and domain databases

Gene3Di1.10.720.30. 1 hit.
3.30.40.10. 1 hit.
InterProiIPR023321. PINIT.
IPR003034. SAP_dom.
IPR027229. SIZ1/SIZ2/Pli1/Gei17.
IPR004181. Znf_MIZ.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PANTHERiPTHR10782:SF4. PTHR10782:SF4. 1 hit.
PfamiPF14324. PINIT. 1 hit.
PF02037. SAP. 1 hit.
PF02891. zf-MIZ. 1 hit.
[Graphical view]
SMARTiSM00513. SAP. 1 hit.
[Graphical view]
PROSITEiPS51466. PINIT. 1 hit.
PS50800. SAP. 1 hit.
PS51044. ZF_SP_RING. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q12216-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASVMSNNNN NNNNNNASYM FTNPLSNTGG GLINEIKDAI NEMEQLKVLE
60 70 80 90 100
LKQICKSLDL SITGKKAVLQ DRIKQFLRKS CDIGHIDPWR PKAIKILIAK
110 120 130 140 150
VRINSSLPKY STLWETLKTG AFKHPVASGQ LPVTALQSTA LPPYSQQQAL
160 170 180 190 200
AYSFTSPFYK PIVQIPDANK KLKQSAGRGC TKMKFKVSKS NHDLLKSNKS
210 220 230 240 250
YKLYLFSGFS IPFIYETVGH EAIDFPYPCE LVFNGTKLED NVKGLKKQNG
260 270 280 290 300
TGNPANLTPY LKVPTEMNHL DLHYLNIDKE YSISCFIVEV FSPEALLGKI
310 320 330 340 350
LKRPKIIKQA TTAYIKRTLN EQDDDDIITT STVLSLQCPI SCTRMKYPAK
360 370 380 390 400
TDQCKHIQCF DALWFLHSQS QVPTWQCPIC QHPIKFDQLK ISEFVDNIIQ
410 420 430 440 450
NCNEDVEQVE ISVDGSWKPI HNSSAVITDT VNQNHSVKNE NQGTVKQEQD
460 470 480 490 500
YDSRNAFDTN LRNGSNHNEP EIISLDSSDD EAFIPASKSF PTHVNPRNDQ
510 520 530 540 550
LRADIFPSES EGSSDYNPNH TSTPKGSPTM DQDNYQDAFQ MRSFLNQGAT
560 570 580 590 600
TNINDTPTNN SSINSFVTAT NGDSRIFYNR GPSTPLLPAV LQNLTNQTEA
610 620 630 640 650
QRNPYGPNYN TTAQDRNLLG IEGDLPPIPP VDPNSEAETE LPTRTTSAAH
660 670 680 690 700
LPPYIHVSTS GHGDDGKIRK RRHSNVSIYI PKNPYATLMK RRPQANHAIM
710 720
NKTLAQTNDF NTSAQDNSEV VDLTSD
Length:726
Mass (Da):81,178
Last modified:November 1, 1996 - v1
Checksum:i74F0F839C5E74BAB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U33152 Genomic DNA. Translation: AAA86121.1.
U55020 Genomic DNA. Translation: AAC49642.1.
Z75064 Genomic DNA. Translation: CAA99362.1.
BK006948 Genomic DNA. Translation: DAA10929.1.
PIRiS67044.
RefSeqiNP_014799.1. NM_001183575.1.

Genome annotation databases

EnsemblFungiiYOR156C; YOR156C; YOR156C.
GeneIDi854327.
KEGGisce:YOR156C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U33152 Genomic DNA. Translation: AAA86121.1.
U55020 Genomic DNA. Translation: AAC49642.1.
Z75064 Genomic DNA. Translation: CAA99362.1.
BK006948 Genomic DNA. Translation: DAA10929.1.
PIRiS67044.
RefSeqiNP_014799.1. NM_001183575.1.

3D structure databases

ProteinModelPortaliQ12216.
SMRiQ12216. Positions 32-120, 156-420.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34552. 157 interactions.
DIPiDIP-5775N.
IntActiQ12216. 12 interactions.
MINTiMINT-582835.
STRINGi4932.YOR156C.

Proteomic databases

PaxDbiQ12216.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYOR156C; YOR156C; YOR156C.
GeneIDi854327.
KEGGisce:YOR156C.

Organism-specific databases

CYGDiYOR156c.
EuPathDBiFungiDB:YOR156C.
SGDiS000005682. NFI1.

Phylogenomic databases

eggNOGiNOG125513.
GeneTreeiENSGT00550000074410.
InParanoidiQ12216.
OrthoDBiEOG7HHX2J.

Enzyme and pathway databases

UniPathwayiUPA00886.
BioCyciYEAST:G3O-33673-MONOMER.

Miscellaneous databases

NextBioi976375.
PROiQ12216.

Gene expression databases

GenevestigatoriQ12216.

Family and domain databases

Gene3Di1.10.720.30. 1 hit.
3.30.40.10. 1 hit.
InterProiIPR023321. PINIT.
IPR003034. SAP_dom.
IPR027229. SIZ1/SIZ2/Pli1/Gei17.
IPR004181. Znf_MIZ.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PANTHERiPTHR10782:SF4. PTHR10782:SF4. 1 hit.
PfamiPF14324. PINIT. 1 hit.
PF02037. SAP. 1 hit.
PF02891. zf-MIZ. 1 hit.
[Graphical view]
SMARTiSM00513. SAP. 1 hit.
[Graphical view]
PROSITEiPS51466. PINIT. 1 hit.
PS50800. SAP. 1 hit.
PS51044. ZF_SP_RING. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "S. cerevisiae two-hybrid interactor with the C-terminus of Cdc12p."
    De Virgilio C., Pringle J.R.
    Submitted (SEP-1995) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. "Analysis of a 35.6 kb region on the right arm of Saccharomyces cerevisiae chromosome XV."
    Bordonne R., Camasses A., Madania A., Poch O., Tarassov I.A., Winsor B., Martin R.P.
    Yeast 13:73-83(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: S288c / FY1678.
  3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XV."
    Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D.
    , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.
    Nature 387:98-102(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. "A novel factor required for the SUMO1/Smt3 conjugation of yeast septins."
    Takahashi Y., Toh-e A., Kikuchi Y.
    Gene 275:223-231(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
  6. "Saccharomyces cerevisiae SMT4 encodes an evolutionarily conserved protease with a role in chromosome condensation regulation."
    Strunnikov A.V., Aravind L., Koonin E.V.
    Genetics 158:95-107(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  7. "Comparative analysis of yeast PIAS-type SUMO ligases in vivo and in vitro."
    Takahashi Y., Toh-e A., Kikuchi Y.
    J. Biochem. 133:415-422(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  8. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  9. "Global analyses of sumoylated proteins in Saccharomyces cerevisiae. Induction of protein sumoylation by cellular stresses."
    Zhou W., Ryan J.J., Zhou H.
    J. Biol. Chem. 279:32262-32268(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUMOYLATION [LARGE SCALE ANALYSIS].
  10. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiSIZ2_YEAST
AccessioniPrimary (citable) accession number: Q12216
Secondary accession number(s): D6W2L3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: April 29, 2015
This is version 111 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 3170 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XV
    Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.