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Q12216 (SIZ2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 104. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
E3 SUMO-protein ligase SIZ2

EC=6.3.2.-
Alternative name(s):
SAP and Miz-finger domain-containing protein 2
Gene names
Name:NFI1
Synonyms:SIZ2
Ordered Locus Names:YOR156C
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length726 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

May act as an E3 ligase mediating SUMO/Smt3 attachment to septins. May be involved in chromosome maintenance. Ref.6 Ref.7

Pathway

Protein modification; protein sumoylation.

Subunit structure

Interacts with CDC12.

Subcellular location

Nucleus Ref.5.

Post-translational modification

Autosumoylated upon ethanol stress.

Miscellaneous

Present with 3170 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the PIAS family.

Contains 1 PINIT domain.

Contains 1 SAP domain.

Contains 1 SP-RING-type zinc finger.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 726726E3 SUMO-protein ligase SIZ2
PRO_0000218985

Regions

Domain43 – 7735SAP
Domain139 – 291153PINIT
Zinc finger323 – 40078SP-RING-type
Compositional bias7 – 1610Poly-Asn

Sequences

Sequence LengthMass (Da)Tools
Q12216 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 74F0F839C5E74BAB

FASTA72681,178
        10         20         30         40         50         60 
MASVMSNNNN NNNNNNASYM FTNPLSNTGG GLINEIKDAI NEMEQLKVLE LKQICKSLDL 

        70         80         90        100        110        120 
SITGKKAVLQ DRIKQFLRKS CDIGHIDPWR PKAIKILIAK VRINSSLPKY STLWETLKTG 

       130        140        150        160        170        180 
AFKHPVASGQ LPVTALQSTA LPPYSQQQAL AYSFTSPFYK PIVQIPDANK KLKQSAGRGC 

       190        200        210        220        230        240 
TKMKFKVSKS NHDLLKSNKS YKLYLFSGFS IPFIYETVGH EAIDFPYPCE LVFNGTKLED 

       250        260        270        280        290        300 
NVKGLKKQNG TGNPANLTPY LKVPTEMNHL DLHYLNIDKE YSISCFIVEV FSPEALLGKI 

       310        320        330        340        350        360 
LKRPKIIKQA TTAYIKRTLN EQDDDDIITT STVLSLQCPI SCTRMKYPAK TDQCKHIQCF 

       370        380        390        400        410        420 
DALWFLHSQS QVPTWQCPIC QHPIKFDQLK ISEFVDNIIQ NCNEDVEQVE ISVDGSWKPI 

       430        440        450        460        470        480 
HNSSAVITDT VNQNHSVKNE NQGTVKQEQD YDSRNAFDTN LRNGSNHNEP EIISLDSSDD 

       490        500        510        520        530        540 
EAFIPASKSF PTHVNPRNDQ LRADIFPSES EGSSDYNPNH TSTPKGSPTM DQDNYQDAFQ 

       550        560        570        580        590        600 
MRSFLNQGAT TNINDTPTNN SSINSFVTAT NGDSRIFYNR GPSTPLLPAV LQNLTNQTEA 

       610        620        630        640        650        660 
QRNPYGPNYN TTAQDRNLLG IEGDLPPIPP VDPNSEAETE LPTRTTSAAH LPPYIHVSTS 

       670        680        690        700        710        720 
GHGDDGKIRK RRHSNVSIYI PKNPYATLMK RRPQANHAIM NKTLAQTNDF NTSAQDNSEV 


VDLTSD 

« Hide

References

« Hide 'large scale' references
[1]"S. cerevisiae two-hybrid interactor with the C-terminus of Cdc12p."
De Virgilio C., Pringle J.R.
Submitted (SEP-1995) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]"Analysis of a 35.6 kb region on the right arm of Saccharomyces cerevisiae chromosome XV."
Bordonne R., Camasses A., Madania A., Poch O., Tarassov I.A., Winsor B., Martin R.P.
Yeast 13:73-83(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: S288c / FY1678.
[3]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XV."
Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D. expand/collapse author list , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.
Nature 387:98-102(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[5]"A novel factor required for the SUMO1/Smt3 conjugation of yeast septins."
Takahashi Y., Toh-e A., Kikuchi Y.
Gene 275:223-231(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION.
[6]"Saccharomyces cerevisiae SMT4 encodes an evolutionarily conserved protease with a role in chromosome condensation regulation."
Strunnikov A.V., Aravind L., Koonin E.V.
Genetics 158:95-107(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[7]"Comparative analysis of yeast PIAS-type SUMO ligases in vivo and in vitro."
Takahashi Y., Toh-e A., Kikuchi Y.
J. Biochem. 133:415-422(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[8]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[9]"Global analyses of sumoylated proteins in Saccharomyces cerevisiae. Induction of protein sumoylation by cellular stresses."
Zhou W., Ryan J.J., Zhou H.
J. Biol. Chem. 279:32262-32268(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: SUMOYLATION [LARGE SCALE ANALYSIS].
[10]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U33152 Genomic DNA. Translation: AAA86121.1.
U55020 Genomic DNA. Translation: AAC49642.1.
Z75064 Genomic DNA. Translation: CAA99362.1.
BK006948 Genomic DNA. Translation: DAA10929.1.
PIRS67044.
RefSeqNP_014799.1. NM_001183575.1.

3D structure databases

ProteinModelPortalQ12216.
SMRQ12216. Positions 32-120, 156-420.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid34552. 148 interactions.
DIPDIP-5775N.
IntActQ12216. 12 interactions.
MINTMINT-582835.
STRING4932.YOR156C.

Proteomic databases

PaxDbQ12216.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYOR156C; YOR156C; YOR156C.
GeneID854327.
KEGGsce:YOR156C.

Organism-specific databases

CYGDYOR156c.
SGDS000005682. NFI1.

Phylogenomic databases

eggNOGNOG125513.
GeneTreeENSGT00550000074410.
OrthoDBEOG7HHX2J.

Enzyme and pathway databases

BioCycYEAST:G3O-33673-MONOMER.
UniPathwayUPA00886.

Gene expression databases

GenevestigatorQ12216.

Family and domain databases

Gene3D1.10.720.30. 1 hit.
3.30.40.10. 1 hit.
InterProIPR023321. PINIT.
IPR003034. SAP_dom.
IPR027229. SIZ1/SIZ2/Pli1/Gei17.
IPR004181. Znf_MIZ.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PANTHERPTHR10782:SF4. PTHR10782:SF4. 1 hit.
PfamPF14324. PINIT. 1 hit.
PF02037. SAP. 1 hit.
PF02891. zf-MIZ. 1 hit.
[Graphical view]
SMARTSM00513. SAP. 1 hit.
[Graphical view]
PROSITEPS51466. PINIT. 1 hit.
PS50800. SAP. 1 hit.
PS51044. ZF_SP_RING. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio976375.

Entry information

Entry nameSIZ2_YEAST
AccessionPrimary (citable) accession number: Q12216
Secondary accession number(s): D6W2L3
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: June 11, 2014
This is version 104 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XV

Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways