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Protein

tRNA pseudouridine synthase 1

Gene

PUS1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Formation of pseudouridine at positions 27 and 28 in the anticodon stem and loop of transfer RNAs; at positions 34 and 36 of intron-containing precursor tRNA(Ile) and at position 35 in the intron-containing tRNA(Tyr).

Catalytic activityi

tRNA uridine = tRNA pseudouridine.

Cofactori

Zn2+Note: Binds 1 zinc ion per subunit.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei134 – 1341NucleophileBy similarity
Binding sitei190 – 1901SubstrateBy similarity

GO - Molecular functioni

  • pseudouridine synthase activity Source: SGD
  • RNA binding Source: InterPro

GO - Biological processi

  • mRNA pseudouridine synthesis Source: SGD
  • snRNA pseudouridine synthesis Source: SGD
  • tRNA modification Source: Reactome
  • tRNA pseudouridine synthesis Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

Zinc

Enzyme and pathway databases

BioCyciYEAST:YPL212C-MONOMER.
BRENDAi5.4.99.B22. 984.
ReactomeiR-SCE-6782315. tRNA modification in the nucleus and cytosol.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA pseudouridine synthase 1 (EC:5.4.99.-)
Alternative name(s):
tRNA pseudouridylate synthase 1
tRNA-uridine isomerase 1
Gene namesi
Name:PUS1
Ordered Locus Names:YPL212C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XVI

Organism-specific databases

EuPathDBiFungiDB:YPL212C.
SGDiS000006133. PUS1.

Subcellular locationi

GO - Cellular componenti

  • nucleoplasm Source: Reactome
  • nucleus Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 544544tRNA pseudouridine synthase 1PRO_0000057530Add
BLAST

Proteomic databases

MaxQBiQ12211.

Interactioni

Protein-protein interaction databases

BioGridi35973. 40 interactions.
DIPiDIP-5344N.
IntActiQ12211. 24 interactions.
MINTiMINT-513680.

Structurei

3D structure databases

ProteinModelPortaliQ12211.
SMRiQ12211. Positions 73-199, 282-458.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00510000047706.
HOGENOMiHOG000213984.
InParanoidiQ12211.
KOiK06173.
OMAiNYTVQKS.
OrthoDBiEOG092C2LVK.

Family and domain databases

Gene3Di3.30.70.580. 1 hit.
3.30.70.660. 1 hit.
InterProiIPR020103. PsdUridine_synth_cat_dom.
IPR001406. PsdUridine_synth_TruA.
IPR020097. PsdUridine_synth_TruA_a/b_dom.
IPR020095. PsdUridine_synth_TruA_C.
IPR020094. PsdUridine_synth_TruA_N.
[Graphical view]
PANTHERiPTHR11142. PTHR11142. 2 hits.
PfamiPF01416. PseudoU_synth_1. 1 hit.
[Graphical view]
SUPFAMiSSF55120. SSF55120. 2 hits.
TIGRFAMsiTIGR00071. hisT_truA. 1 hit.

Sequencei

Sequence statusi: Complete.

Q12211-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSEENLRPAY DDQVNEDVYK RGAQSKLTKA RKADFDDEKD KKKDNDKHID
60 70 80 90 100
KRPKSGPRLD ENGNPLPKEP RLPKRKVAVM VGYCGTGYHG MQYNPPNPTI
110 120 130 140 150
ESALFKAFVE AGAISKDNSN DLKKNGFMRA ARTDKGVHAG GNLISLKMII
160 170 180 190 200
EDPDIKQKIN EKLPEGIRVW DIERVNKAFD CRKMCSSRWY EYLLPTYSLI
210 220 230 240 250
GPKPGSILYR DIEESKTELP GVLDEDLESK EFWEEFKKDA NEKFSTEEIE
260 270 280 290 300
AILAYVPPAR DEFDINEELY QKVKKYKQLE NAHRRRYRIS AAKLAKFRAS
310 320 330 340 350
TSQYLGAHNF HNFTLGKDFK EPSAIRFMKD IKVSDPFVIG DAQTEWISIK
360 370 380 390 400
IHGQSFMLHQ IRKMVSMATL ITRCGCPVER ISQAYGQQKI NIPKAPALGL
410 420 430 440 450
LLEAPVFEGY NKRLEQFGYK AIDFSKYQDE VDKFKMKHIY DKIYKEEVDE
460 470 480 490 500
NVFNAFFSYI DSFNKVTGAQ GEETADKSGP AVQKSIFEFL TAKGIPGLTD
510 520 530 540
APESNKKIKQ RKRMEEEEAA SKKAEISSTT QSNEPEVQPE AAAN
Length:544
Mass (Da):62,143
Last modified:November 1, 1996 - v1
Checksum:i1A7E449601C26EFD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X80673 Genomic DNA. Translation: CAA56698.1.
Z73568 Genomic DNA. Translation: CAA97927.1.
BK006949 Genomic DNA. Translation: DAA11224.1.
PIRiS65231.
RefSeqiNP_015112.1. NM_001184026.1.

Genome annotation databases

EnsemblFungiiYPL212C; YPL212C; YPL212C.
GeneIDi855889.
KEGGisce:YPL212C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X80673 Genomic DNA. Translation: CAA56698.1.
Z73568 Genomic DNA. Translation: CAA97927.1.
BK006949 Genomic DNA. Translation: DAA11224.1.
PIRiS65231.
RefSeqiNP_015112.1. NM_001184026.1.

3D structure databases

ProteinModelPortaliQ12211.
SMRiQ12211. Positions 73-199, 282-458.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi35973. 40 interactions.
DIPiDIP-5344N.
IntActiQ12211. 24 interactions.
MINTiMINT-513680.

Proteomic databases

MaxQBiQ12211.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYPL212C; YPL212C; YPL212C.
GeneIDi855889.
KEGGisce:YPL212C.

Organism-specific databases

EuPathDBiFungiDB:YPL212C.
SGDiS000006133. PUS1.

Phylogenomic databases

GeneTreeiENSGT00510000047706.
HOGENOMiHOG000213984.
InParanoidiQ12211.
KOiK06173.
OMAiNYTVQKS.
OrthoDBiEOG092C2LVK.

Enzyme and pathway databases

BioCyciYEAST:YPL212C-MONOMER.
BRENDAi5.4.99.B22. 984.
ReactomeiR-SCE-6782315. tRNA modification in the nucleus and cytosol.

Miscellaneous databases

PROiQ12211.

Family and domain databases

Gene3Di3.30.70.580. 1 hit.
3.30.70.660. 1 hit.
InterProiIPR020103. PsdUridine_synth_cat_dom.
IPR001406. PsdUridine_synth_TruA.
IPR020097. PsdUridine_synth_TruA_a/b_dom.
IPR020095. PsdUridine_synth_TruA_C.
IPR020094. PsdUridine_synth_TruA_N.
[Graphical view]
PANTHERiPTHR11142. PTHR11142. 2 hits.
PfamiPF01416. PseudoU_synth_1. 1 hit.
[Graphical view]
SUPFAMiSSF55120. SSF55120. 2 hits.
TIGRFAMsiTIGR00071. hisT_truA. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiPUS1_YEAST
AccessioniPrimary (citable) accession number: Q12211
Secondary accession number(s): D6W3F8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: November 1, 1996
Last modified: September 7, 2016
This is version 129 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 8130 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XVI
    Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.