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Protein

Probable ferric reductase transmembrane component 8

Gene

FRE8

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Required for the uptake of Fe3+ ions. May participate in the transport of electrons from cytoplasm to an extracellular substrate (Fe3+ ion) via FAD and heme intermediates (By similarity). Involved in iron homeostasis.By similarity1 Publication

Catalytic activityi

2 Fe(II)-siderophore + NAD+ + H+ = 2 Fe(III)-siderophore + NADH.

Cofactori

FADCurated

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi320 – 3267FADSequence analysis
Nucleotide bindingi442 – 4509NADSequence analysis

GO - Molecular functioni

  • ferric-chelate reductase activity Source: UniProtKB-EC
  • oxidoreductase activity, oxidizing metal ions Source: SGD

GO - Biological processi

  • cellular metal ion homeostasis Source: SGD
  • ion transport Source: UniProtKB-KW
  • iron ion homeostasis Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Ion transport, Iron transport, Transport

Keywords - Ligandi

FAD, Flavoprotein, Iron, NAD

Enzyme and pathway databases

BioCyciYEAST:G3O-32203-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable ferric reductase transmembrane component 8 (EC:1.16.1.7)
Alternative name(s):
Ferric-chelate reductase 8
Gene namesi
Name:FRE8
Ordered Locus Names:YLR047C
ORF Names:L2114
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XII

Organism-specific databases

EuPathDBiFungiDB:YLR047C.
SGDiS000004037. FRE8.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 3535CytoplasmicSequence analysisAdd
BLAST
Transmembranei36 – 5621HelicalSequence analysisAdd
BLAST
Topological domaini57 – 8933ExtracellularSequence analysisAdd
BLAST
Transmembranei90 – 11021HelicalSequence analysisAdd
BLAST
Topological domaini111 – 12818CytoplasmicSequence analysisAdd
BLAST
Transmembranei129 – 14921HelicalSequence analysisAdd
BLAST
Topological domaini150 – 1578ExtracellularSequence analysis
Transmembranei158 – 17821HelicalSequence analysisAdd
BLAST
Topological domaini179 – 1868CytoplasmicSequence analysis
Transmembranei187 – 20721HelicalSequence analysisAdd
BLAST
Topological domaini208 – 2169ExtracellularSequence analysis
Transmembranei217 – 23721HelicalSequence analysisAdd
BLAST
Topological domaini238 – 26730CytoplasmicSequence analysisAdd
BLAST
Transmembranei268 – 28821HelicalSequence analysisAdd
BLAST
Topological domaini289 – 402114ExtracellularSequence analysisAdd
BLAST
Transmembranei403 – 42321HelicalSequence analysisAdd
BLAST
Topological domaini424 – 686263CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • cell Source: GOC
  • integral component of membrane Source: UniProtKB-KW
  • membrane Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 686686Probable ferric reductase transmembrane component 8PRO_0000239647Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi371 – 3711N-linked (GlcNAc...)Sequence analysis
Glycosylationi383 – 3831N-linked (GlcNAc...)Sequence analysis
Glycosylationi384 – 3841N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiQ12209.

PTM databases

iPTMnetiQ12209.

Expressioni

Inductioni

Expression decreased by the copper-sensing transcription factor MAC1.1 Publication

Interactioni

Protein-protein interaction databases

BioGridi31322. 8 interactions.
IntActiQ12209. 1 interaction.

Structurei

3D structure databases

ProteinModelPortaliQ12209.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini119 – 666548Ferric oxidoreductaseAdd
BLAST

Sequence similaritiesi

Contains 1 ferric oxidoreductase domain.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00530000068386.
HOGENOMiHOG000112643.
InParanoidiQ12209.
OMAiIPNYHPY.
OrthoDBiEOG7JHMF0.

Family and domain databases

InterProiIPR013130. Fe3_Rdtase_TM_dom.
[Graphical view]
PfamiPF01794. Ferric_reduct. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q12209-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNLKSIVSWF KEHLPSFDVD VDKHFRTLRV RKYSQICLLI SFIIICVIIP
60 70 80 90 100
LMNYLLLTDK FFKICHHLKH HVFNRRSWVH KTHMYHKQSL QLCLICFVFT
110 120 130 140 150
SFFVIQGANG DLLEITKRMG RISVALMPPL LFLTLRPSPL PHTLYLALLP
160 170 180 190 200
LHKWISRIVV LESILHTWFY LYYMYINDTL YVKMRKLPNI YGVIALGLFL
210 220 230 240 250
LIAITSVRYA RRWSYRVFYY VHYVSTWLIL VFLHYHARPG ISYYTTLNVL
260 270 280 290 300
ILTGQIVYRL HITNVTRVTI VPISSSLSLL EFPLTDLPKK PILPGGHLRI
310 320 330 340 350
NIYHRNFLRR FFSHLIPFQH PFTIASIPSD NLVRLIIRNG HFPLRTNEKY
360 370 380 390 400
YITGAFEPEL SFISKPTVPF NITTKSSKNP FRNNSSALIN SPLNFLIKAQ
410 420 430 440 450
RVFMCVGGSG ISFGLPLLRI LNFNGVNVRL LWVSRDYKDL EVLNHFKNNF
460 470 480 490 500
EGMEIYISGT EGNEQDIEID YIDYHDCAAD INDEVRSISS SGRVSELGDN
510 520 530 540 550
SMLSDGNPQP TEPNENTALL SKKSTLRNHH PPKTSDIPDI NADDEIDFTY
560 570 580 590 600
AFSRSKSRKN TAQGTLTTHS SFNGSSVFRQ PKIIEPPAQD PCLEAAPKKI
610 620 630 640 650
RIPAGVKVFF GRPTLGDKDY EWCLQTECDA ETDSIQCCRW ANQGRDHAEY
660 670 680
LSQVWVLAAG PRGLIESTKR WATDGGLHFH GESFAL
Length:686
Mass (Da):78,949
Last modified:November 1, 1996 - v1
Checksum:i907EDDE10D7B9BA2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X94607 Genomic DNA. Translation: CAA64294.1.
Z73219 Genomic DNA. Translation: CAA97577.1.
BK006945 Genomic DNA. Translation: DAA09365.1.
PIRiS61621.
RefSeqiNP_013148.1. NM_001181934.1.

Genome annotation databases

EnsemblFungiiYLR047C; YLR047C; YLR047C.
GeneIDi850736.
KEGGisce:YLR047C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X94607 Genomic DNA. Translation: CAA64294.1.
Z73219 Genomic DNA. Translation: CAA97577.1.
BK006945 Genomic DNA. Translation: DAA09365.1.
PIRiS61621.
RefSeqiNP_013148.1. NM_001181934.1.

3D structure databases

ProteinModelPortaliQ12209.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi31322. 8 interactions.
IntActiQ12209. 1 interaction.

PTM databases

iPTMnetiQ12209.

Proteomic databases

MaxQBiQ12209.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYLR047C; YLR047C; YLR047C.
GeneIDi850736.
KEGGisce:YLR047C.

Organism-specific databases

EuPathDBiFungiDB:YLR047C.
SGDiS000004037. FRE8.

Phylogenomic databases

GeneTreeiENSGT00530000068386.
HOGENOMiHOG000112643.
InParanoidiQ12209.
OMAiIPNYHPY.
OrthoDBiEOG7JHMF0.

Enzyme and pathway databases

BioCyciYEAST:G3O-32203-MONOMER.

Miscellaneous databases

PROiQ12209.

Family and domain databases

InterProiIPR013130. Fe3_Rdtase_TM_dom.
[Graphical view]
PfamiPF01794. Ferric_reduct. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XII."
    Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H.
    , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D.
    Nature 387:87-90(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. Cited for: FUNCTION, INDUCTION.
  4. "A global topology map of the Saccharomyces cerevisiae membrane proteome."
    Kim H., Melen K., Oesterberg M., von Heijne G.
    Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
    Strain: ATCC 208353 / W303-1A.

Entry informationi

Entry nameiFRE8_YEAST
AccessioniPrimary (citable) accession number: Q12209
Secondary accession number(s): D6VY49
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 13, 2006
Last sequence update: November 1, 1996
Last modified: June 8, 2016
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XII
    Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.