Reviewed, UniProtKB/Swiss-Prot Q12209 (FRE8_YEAST)

Names and origin

Protein names

Recommended name:
    Probable ferric reductase transmembrane component 8
    EC=1.16.1.7
Alternative name(s):
    Ferric-chelate reductase 8

Gene names

Name:	FRE8
Ordered Locus Names:	YLR047C
ORF Names:	L2114

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	686 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Required for the uptake of Fe3+ ions. May participate in the transport of electrons from cytoplasm to an extracellular substrate (Fe3+ ion) via FAD and heme intermediates By similarity. Involved in iron homeostasis.
Catalytic activity	2 Fe2+ + NAD+ = 2 Fe3+ + NADH.
Cofactor	FAD Probable.
Subcellular location	Membrane; Multi-pass membrane protein.
Induction	Expression decreased by the copper-sensing transcription factor MAC1. Ref.2
Sequence similarities	Contains 1 ferric oxidoreductase domain.

Ontologies

Keywords
Biological process	Electron transport Ion transport Iron transport Transport
Cellular component	Membrane
Domain	Transmembrane
Ligand	FAD Iron NAD
Molecular function	Oxidoreductase
PTM	Glycoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cellular metal ion homeostasis Ref.2 Inferred from mutant phenotype. Source: SGD electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW iron ion transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	FAD binding Inferred from electronic annotation. Source: InterPro electron carrier activity Inferred from electronic annotation. Source: InterPro ferric-chelate reductase activity Inferred from electronic annotation. Source: EC iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 686	686	Probable ferric reductase transmembrane component 8		PRO_0000239647
Regions
	Topological domain	1 – 35	35	Cytoplasmic Potential
	Transmembrane	36 – 56	21	Potential
	Topological domain	57 – 89	33	Extracellular Potential
	Transmembrane	90 – 110	21	Potential
	Topological domain	111 – 128	18	Cytoplasmic Potential
	Transmembrane	129 – 149	21	Potential
	Topological domain	150 – 157	8	Extracellular Potential
	Transmembrane	158 – 178	21	Potential
	Topological domain	179 – 186	8	Cytoplasmic Potential
	Transmembrane	187 – 207	21	Potential
	Topological domain	208 – 216	9	Extracellular Potential
	Transmembrane	217 – 237	21	Potential
	Topological domain	238 – 267	30	Cytoplasmic Potential
	Transmembrane	268 – 288	21	Potential
	Topological domain	289 – 402	114	Extracellular Potential
	Transmembrane	403 – 423	21	Potential
	Topological domain	424 – 686	263	Cytoplasmic Potential
	Domain	119 – 666	548	Ferric oxidoreductase
	Nucleotide binding	320 – 326	7	FAD Potential
	Nucleotide binding	442 – 450	9	NAD Potential
Amino acid modifications
	Glycosylation	371	1	N-linked (GlcNAc...) Potential
	Glycosylation	383	1	N-linked (GlcNAc...) Potential
	Glycosylation	384	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q12209-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 907EDDE10D7B9BA2	FASTA	686	78,949
	10 20 30 40 50 60 MNLKSIVSWF KEHLPSFDVD VDKHFRTLRV RKYSQICLLI SFIIICVIIP LMNYLLLTDK 70 80 90 100 110 120 FFKICHHLKH HVFNRRSWVH KTHMYHKQSL QLCLICFVFT SFFVIQGANG DLLEITKRMG 130 140 150 160 170 180 RISVALMPPL LFLTLRPSPL PHTLYLALLP LHKWISRIVV LESILHTWFY LYYMYINDTL 190 200 210 220 230 240 YVKMRKLPNI YGVIALGLFL LIAITSVRYA RRWSYRVFYY VHYVSTWLIL VFLHYHARPG 250 260 270 280 290 300 ISYYTTLNVL ILTGQIVYRL HITNVTRVTI VPISSSLSLL EFPLTDLPKK PILPGGHLRI 310 320 330 340 350 360 NIYHRNFLRR FFSHLIPFQH PFTIASIPSD NLVRLIIRNG HFPLRTNEKY YITGAFEPEL 370 380 390 400 410 420 SFISKPTVPF NITTKSSKNP FRNNSSALIN SPLNFLIKAQ RVFMCVGGSG ISFGLPLLRI 430 440 450 460 470 480 LNFNGVNVRL LWVSRDYKDL EVLNHFKNNF EGMEIYISGT EGNEQDIEID YIDYHDCAAD 490 500 510 520 530 540 INDEVRSISS SGRVSELGDN SMLSDGNPQP TEPNENTALL SKKSTLRNHH PPKTSDIPDI 550 560 570 580 590 600 NADDEIDFTY AFSRSKSRKN TAQGTLTTHS SFNGSSVFRQ PKIIEPPAQD PCLEAAPKKI 610 620 630 640 650 660 RIPAGVKVFF GRPTLGDKDY EWCLQTECDA ETDSIQCCRW ANQGRDHAEY LSQVWVLAAG 670 680 PRGLIESTKR WATDGGLHFH GESFAL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XII." Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H. , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D. Nature 387:87-90(1997) [PubMed: 9169871] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	"Exploratory and confirmatory gene expression profiling of mac1Delta." De Freitas J.M., Kim J.H., Poynton H., Su T., Wintz H., Fox T., Holman P., Loguinov A., Keles S., van der Laan M., Vulpe C. J. Biol. Chem. 279:4450-4458(2004) [PubMed: 14534306] [Abstract] Cited for: FUNCTION, INDUCTION.
[3]	"A global topology map of the Saccharomyces cerevisiae membrane proteome." Kim H., Melen K., Oesterberg M., von Heijne G. Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed: 16847258] [Abstract] Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X94607 Genomic DNA. Translation: CAA64294.1. Z73219 Genomic DNA. Translation: CAA97577.1.
PIR	S61621.
RefSeq	NP_013148.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	Q12209. 1 interaction.
Genome annotation databases
Ensembl	YLR047C. Saccharomyces cerevisiae. [Contig view]
GeneID	850736.
GenomeReviews	Gene locus YLR047C in contig Y13138_GR.
KEGG	sce:YLR047C.
NMPDR	fig|4932.3.peg.4139.
Organism-specific databases
CYGD	YLR047c.
SGD	S000004037. FRE8.
Yeast-GFP	Search...
Phylogenomic databases
HOGENOM	Q12209.
OMA	Q12209. FHEEAFY.
Enzyme and pathway databases
BRENDA	1.16.1.7. 250.
Gene expression databases
ArrayExpress	Q12209.
GermOnline	YLR047C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR013130. Fe3_reduct_TM_N. [Graphical view]
Pfam	PF01794. Ferric_reduct. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	966840.

Entry information

Entry name

FRE8_YEAST

Accession

Primary (citable) accession number: Q12209

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 13, 2006
Last sequence update:	November 1, 1996
Last modified:	June 16, 2009
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)