Probable ferric reductase transmembrane component 8 - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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Q12209 (FRE8_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified December 14, 2011. Version 72. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Probable ferric reductase transmembrane component 8
EC=1.16.1.7

Alternative name(s):
Ferric-chelate reductase 8

Gene names

Name:	FRE8
Ordered Locus Names:	YLR047C
ORF Names:	L2114

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	686 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Required for the uptake of Fe³⁺ ions. May participate in the transport of electrons from cytoplasm to an extracellular substrate (Fe³⁺ ion) via FAD and heme intermediates By similarity. Involved in iron homeostasis. Ref.3
Catalytic activity	2 Fe²⁺ + NAD⁺ + H⁺ = 2 Fe³⁺ + NADH.
Cofactor	FAD Probable.
Subcellular location	Membrane; Multi-pass membrane protein.
Induction	Expression decreased by the copper-sensing transcription factor MAC1. Ref.3
Sequence similarities	Contains 1 ferric oxidoreductase domain.

Ontologies

Keywords
Biological process	Electron transport Ion transport Iron transport Transport
Cellular component	Membrane
Domain	Transmembrane Transmembrane helix
Ligand	FAD Iron NAD
Molecular function	Oxidoreductase
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cellular metal ion homeostasis Inferred from mutant phenotype Ref.3. Source: SGD electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW ion transport Inferred from electronic annotation. Source: UniProtKB-KW iron ion homeostasis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	electron carrier activity Inferred from electronic annotation. Source: InterPro ferric-chelate reductase activity Inferred from electronic annotation. Source: EC flavin adenine dinucleotide binding Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 686

686

Probable ferric reductase transmembrane component 8

PRO_0000239647

Regions

Topological domain

1 – 35

Cytoplasmic Potential

Transmembrane

36 – 56

Helical; Potential

Topological domain

57 – 89

Extracellular Potential

Transmembrane

90 – 110

Helical; Potential

Topological domain

111 – 128

Cytoplasmic Potential

Transmembrane

129 – 149

Helical; Potential

Topological domain

150 – 157

Extracellular Potential

Transmembrane

158 – 178

Helical; Potential

Topological domain

179 – 186

Cytoplasmic Potential

Transmembrane

187 – 207

Helical; Potential

Topological domain

208 – 216

Extracellular Potential

Transmembrane

217 – 237

Helical; Potential

Topological domain

238 – 267

Cytoplasmic Potential

Transmembrane

268 – 288

Helical; Potential

Topological domain

289 – 402

114

Extracellular Potential

Transmembrane

403 – 423

Helical; Potential

Topological domain

424 – 686

263

Cytoplasmic Potential

Domain

119 – 666

548

Ferric oxidoreductase

Nucleotide binding

320 – 326

FAD Potential

Nucleotide binding

442 – 450

NAD Potential

Amino acid modifications

Glycosylation

371

N-linked (GlcNAc...) Potential

Glycosylation

383

N-linked (GlcNAc...) Potential

Glycosylation

384

N-linked (GlcNAc...) Potential

Sequences

Sequence

Length

Mass (Da)

Tools

Q12209 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 907EDDE10D7B9BA2
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FASTA

686

78,949

        10         20         30         40         50         60 
MNLKSIVSWF KEHLPSFDVD VDKHFRTLRV RKYSQICLLI SFIIICVIIP LMNYLLLTDK 

        70         80         90        100        110        120 
FFKICHHLKH HVFNRRSWVH KTHMYHKQSL QLCLICFVFT SFFVIQGANG DLLEITKRMG 

       130        140        150        160        170        180 
RISVALMPPL LFLTLRPSPL PHTLYLALLP LHKWISRIVV LESILHTWFY LYYMYINDTL 

       190        200        210        220        230        240 
YVKMRKLPNI YGVIALGLFL LIAITSVRYA RRWSYRVFYY VHYVSTWLIL VFLHYHARPG 

       250        260        270        280        290        300 
ISYYTTLNVL ILTGQIVYRL HITNVTRVTI VPISSSLSLL EFPLTDLPKK PILPGGHLRI 

       310        320        330        340        350        360 
NIYHRNFLRR FFSHLIPFQH PFTIASIPSD NLVRLIIRNG HFPLRTNEKY YITGAFEPEL 

       370        380        390        400        410        420 
SFISKPTVPF NITTKSSKNP FRNNSSALIN SPLNFLIKAQ RVFMCVGGSG ISFGLPLLRI 

       430        440        450        460        470        480 
LNFNGVNVRL LWVSRDYKDL EVLNHFKNNF EGMEIYISGT EGNEQDIEID YIDYHDCAAD 

       490        500        510        520        530        540 
INDEVRSISS SGRVSELGDN SMLSDGNPQP TEPNENTALL SKKSTLRNHH PPKTSDIPDI 

       550        560        570        580        590        600 
NADDEIDFTY AFSRSKSRKN TAQGTLTTHS SFNGSSVFRQ PKIIEPPAQD PCLEAAPKKI 

       610        620        630        640        650        660 
RIPAGVKVFF GRPTLGDKDY EWCLQTECDA ETDSIQCCRW ANQGRDHAEY LSQVWVLAAG 

       670        680 
PRGLIESTKR WATDGGLHFH GESFAL

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XII." Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H. Hoheisel J.D. Nature 387:87-90(1997) [PubMed: 9169871] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Exploratory and confirmatory gene expression profiling of mac1Delta." De Freitas J.M., Kim J.H., Poynton H., Su T., Wintz H., Fox T., Holman P., Loguinov A., Keles S., van der Laan M., Vulpe C. J. Biol. Chem. 279:4450-4458(2004) [PubMed: 14534306] [Abstract] Cited for: FUNCTION, INDUCTION.
[4]	"A global topology map of the Saccharomyces cerevisiae membrane proteome." Kim H., Melen K., Oesterberg M., von Heijne G. Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed: 16847258] [Abstract] Cited for: TOPOLOGY [LARGE SCALE ANALYSIS]. Strain: ATCC 208353 / W303-1A.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X94607 Genomic DNA. Translation: CAA64294.1. Z73219 Genomic DNA. Translation: CAA97577.1. BK006945 Genomic DNA. Translation: DAA09365.1.
PIR	S61621.
RefSeq	NP_013148.1. NM_001181934.1.
3D structure databases
ProteinModelPortal	Q12209.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q12209. 1 interaction.
STRING	Q12209.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YLR047C; YLR047C; YLR047C.
GeneID	850736.
KEGG	sce:YLR047C.
NMPDR	fig\|4932.3.peg.4139.
Organism-specific databases
CYGD	YLR047c.
SGD	S000004037. FRE8.
Phylogenomic databases
eggNOG	fuNOG05695.
GeneTree	EFGT00050000003069.
HOGENOM	HBG202425.
OMA	CINEGFT.
OrthoDB	EOG49CTHG.
Gene expression databases
ArrayExpress	Q12209.
Genevestigator	Q12209.
GermOnline	YLR047C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR013130. Flavoprotein_TM. [Graphical view]
Pfam	PF01794. Ferric_reduct. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	966840.

Entry information

Entry name

FRE8_YEAST

Accession

Primary (citable) accession number: Q12209
Secondary accession number(s): D6VY49

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 13, 2006
Last sequence update:	November 1, 1996
Last modified:	December 14, 2011
This is version 72 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents