Q12208 (YL132_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Uncharacterized protein YLR132C

Gene names

Ordered Locus Names:	YLR132C
ORF Names:	L3127

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	290 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Appears to be a bifunctional protein that may be involved in mRNA splicing and aerobic respiration. Ref.3
Subunit structure	Interacts with the pre-mRNA-splicing factor PRP19 in the nucleus. Interacts with the cytochrome b-c1 complex subunit COR1 in the mitochondrion. Ref.3
Subcellular location	Nucleus. Mitochondrion Ref.3 Ref.4.
Miscellaneous	Present with 486 molecules/cell in log phase SD medium. Ref.5

Ontologies

Keywords
Biological process	mRNA processing mRNA splicing
Cellular component	Mitochondrion Nucleus
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	RNA splicing Inferred from electronic annotation. Source: UniProtKB-KW mRNA processing Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	mitochondrion Inferred from direct assay Ref.4Ref.3. Source: SGD nucleus Inferred from direct assay Ref.4Ref.3. Source: SGD
Molecular function	protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
PRP19	P32523	3	EBI-31589,EBI-493

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 290	290	Uncharacterized protein YLR132C		PRO_0000262872
Amino acid modifications
	Modified residue	42	1	Phosphothreonine Ref.6

Sequences

	Sequence		Length	Mass (Da)
	Q12208 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 63BCE8C433136FB5	FASTA	290	33,694
	10 20 30 40 50 60 MEFISADYSS SDGSDTESES SNKSEVQIEY TEKTCIQKAD STDLPAIPDS IILKYHIPPN 70 80 90 100 110 120 LQKYEHQDMN MSRFWRSFTY FEWRPTPAIH RQLQKIICKY KETFMKQEYT NPYQLVDFDP 130 140 150 160 170 180 LFISHLGAPK PLHVSLTRSL LFETEEQRHV FIQEMRNGLR NNEITPFKLQ ICSYPKLYIS 190 200 210 220 230 240 ERANTLYLGL PVSECPNKAQ ISPFKTIIAE ALQKSGISNY QDLIVSRQNL HVSIAIASNP 250 260 270 280 290 SKATLKRYQQ LNETMGALLL LNNDFAYKLE FLVNSIYCDE NRHSIRIPFN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XII." Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H. , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D. Nature 387:87-90(1997) [PubMed: 9169871] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Assigning function to yeast proteins by integration of technologies." Hazbun T.R., Malmstroem L., Anderson S., Graczyk B.J., Fox B., Riffle M., Sundin B.A., Aranda J.D., McDonald W.H., Chiu C.-H., Snydsman B.E., Bradley P., Muller E.G.D., Fields S., Baker D., Yates J.R. III, Davis T.N. Mol. Cell 12:1353-1365(2003) [PubMed: 14690591] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH COR1 AND PRP19.
[4]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-42, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U53881 Genomic DNA. Translation: AAB82399.1. X91258 Genomic DNA. Translation: CAA62645.1. Z73304 Genomic DNA. Translation: CAA97703.1. BK006945 Genomic DNA. Translation: DAA09443.1.
PIR	S59322.
RefSeq	NP_013233.1. NM_001182019.1.
3D structure databases
ProteinModelPortal	Q12208.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-2147N.
IntAct	Q12208. 13 interactions.
MINT	MINT-2492570.
STRING	Q12208.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YLR132C; YLR132C; YLR132C.
GeneID	850823.
KEGG	sce:YLR132C.
NMPDR	fig|4932.3.peg.4226.
Organism-specific databases
CYGD	YLR132c.
SGD	S000004122. YLR132C.
Phylogenomic databases
eggNOG	fuNOG12962.
OMA	TEREMIS.
OrthoDB	EOG4HMNM3.
Gene expression databases
ArrayExpress	Q12208.
Genevestigator	Q12208.
GermOnline	YLR132C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR019146. UPF0406. [Graphical view]
Pfam	PF09749. HVSL. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	967076.

Entry information

Entry name

YL132_YEAST

Accession

Primary (citable) accession number: Q12208
Secondary accession number(s): D6VYC7

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 28, 2006
Last sequence update:	November 1, 1996
Last modified:	December 14, 2011
This is version 71 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program