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Protein

Putative endoplasmic reticulum mannosidase MNL2

Gene

MNL2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Putative mannosidase involved in glycoprotein quality control since it is involved in the targeting of misfolded glycoproteins for ER-associated protein degradation (ERAD).1 Publication

Cofactori

Ca2+By similarity

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.By similarity
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

GO - Molecular functioni

GO - Biological processi

  • ER-associated ubiquitin-dependent protein catabolic process Source: SGD
  • protein glycosylation Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Enzyme and pathway databases

BioCyciYEAST:G3O-32212-MONOMER.
UniPathwayiUPA00378.

Protein family/group databases

CAZyiGH47. Glycoside Hydrolase Family 47.

Names & Taxonomyi

Protein namesi
Recommended name:
Putative endoplasmic reticulum mannosidase MNL2 (EC:3.2.1.-By similarity)
Alternative name(s):
Mannosidase-like protein 2
Gene namesi
Name:MNL2
Ordered Locus Names:YLR057W
ORF Names:L2153
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XII

Organism-specific databases

EuPathDBiFungiDB:YLR057W.
SGDiS000004047. MNL2.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 1212CytoplasmicSequence analysisAdd
BLAST
Transmembranei13 – 3220Helical; Signal-anchor for type II membrane proteinSequence analysisAdd
BLAST
Topological domaini33 – 849817LumenalSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 849849Putative endoplasmic reticulum mannosidase MNL2PRO_0000247229Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi45 – 451N-linked (GlcNAc...)Sequence analysis
Disulfide bondi559 ↔ 598By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

MaxQBiQ12205.

PTM databases

iPTMnetiQ12205.

Interactioni

Protein-protein interaction databases

BioGridi31332. 30 interactions.
DIPiDIP-8921N.
MINTiMINT-499842.

Structurei

3D structure databases

ProteinModelPortaliQ12205.
SMRiQ12205. Positions 166-332, 739-844.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 47 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

HOGENOMiHOG000142372.
InParanoidiQ12205.
KOiK01230.
OMAiYRRYRAF.
OrthoDBiEOG7SR4XT.

Family and domain databases

Gene3Di1.50.10.50. 4 hits.
InterProiIPR001382. Glyco_hydro_47.
[Graphical view]
PfamiPF01532. Glyco_hydro_47. 1 hit.
[Graphical view]
PRINTSiPR00747. GLYHDRLASE47.
SUPFAMiSSF48225. SSF48225. 4 hits.

Sequencei

Sequence statusi: Complete.

Q12205-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSIARLVYSL FRRVRSVLLL FITISLLFYY TFQNEIDILN SYALNDSLPS
60 70 80 90 100
INNYEHNTEG SSKLDPPDLS STGSDRIATD KENGNVAVDL SDPATLREKN
110 120 130 140 150
KYFPLLLKGS SHQIGSNLPI SSLLTYKEKY PVLFEYSSPS LTSISQNDVH
160 170 180 190 200
KIQPAMQLPP DVDMIKQIKD IFMKSWNQEQ LLLKSNLRRE STWPIDLIDS
210 220 230 240 250
LDTLYLCGET KLFQDSVNII EDFDFRVPPL AMEVIDIPDI TTRVLEGLLS
260 270 280 290 300
AYELSMDKRL LNKAKHVADF ILRSFDTPNR IPILKYFWKS DLRNRFPDRT
310 320 330 340 350
VPSGQLTTMA LAFIRLSQLT RLNKYFDAVE RVFTTIRQSY NEFDMEFMLP
360 370 380 390 400
DVVDASGCQL LTQEEIENGA HLKGSSIMKS INENFKFVHC QQLGKFLNPP
410 420 430 440 450
IDDNSLQEQS QYQAYRINEK TVPILENLFK INDLFQSSYD ILDGSSKNAN
460 470 480 490 500
AATMDPSIGS EVEAVDEIIE KRNFKDGTKK DSTKNTVGDK SLIDSQTFLT
510 520 530 540 550
NSISNIFKFM TFRPMLPKQT ENKKFNFLNS ILTKSQFMPT TNELDVTIRK
560 570 580 590 600
SYDVSLYSCR LGGILGLSSR VPHRGGVNTK YILPSSLLEM SEIITESCFM
610 620 630 640 650
LMEEFDGLLP QKFELDPCTD ETNGNCEFNG ETKSRMIANG EYETFENDLD
660 670 680 690 700
VGIKVSNYGK GGNDQKAKRN VLSKDGITET QNIKGDTVGS SKSIAEIDGD
710 720 730 740 750
EVTQIRRVFT LGKDIKPHIT TDDTMGSQWK NHPDWPFWVN KVESRRLLDS
760 770 780 790 800
NIIESIFYMY RISGEQKWRS MGKQSFGILM QELMELNSGA KGLWQIKEFY
810 820 830 840
ENGEKVNNDL PSYWFSRTLK YYLLLFSDGD KVSLDKHILT QGGHIIKKK
Length:849
Mass (Da):96,997
Last modified:November 1, 1996 - v1
Checksum:iA6B87AC32936A0D5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X94607 Genomic DNA. Translation: CAA64304.1.
Z73229 Genomic DNA. Translation: CAA97587.1.
Z73230 Genomic DNA. Translation: CAA97589.1.
AY692557 Genomic DNA. Translation: AAT92576.1.
BK006945 Genomic DNA. Translation: DAA09375.1.
PIRiS61631.
RefSeqiNP_013158.1. NM_001181944.1.

Genome annotation databases

EnsemblFungiiYLR057W; YLR057W; YLR057W.
GeneIDi850746.
KEGGisce:YLR057W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X94607 Genomic DNA. Translation: CAA64304.1.
Z73229 Genomic DNA. Translation: CAA97587.1.
Z73230 Genomic DNA. Translation: CAA97589.1.
AY692557 Genomic DNA. Translation: AAT92576.1.
BK006945 Genomic DNA. Translation: DAA09375.1.
PIRiS61631.
RefSeqiNP_013158.1. NM_001181944.1.

3D structure databases

ProteinModelPortaliQ12205.
SMRiQ12205. Positions 166-332, 739-844.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi31332. 30 interactions.
DIPiDIP-8921N.
MINTiMINT-499842.

Protein family/group databases

CAZyiGH47. Glycoside Hydrolase Family 47.

PTM databases

iPTMnetiQ12205.

Proteomic databases

MaxQBiQ12205.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYLR057W; YLR057W; YLR057W.
GeneIDi850746.
KEGGisce:YLR057W.

Organism-specific databases

EuPathDBiFungiDB:YLR057W.
SGDiS000004047. MNL2.

Phylogenomic databases

HOGENOMiHOG000142372.
InParanoidiQ12205.
KOiK01230.
OMAiYRRYRAF.
OrthoDBiEOG7SR4XT.

Enzyme and pathway databases

UniPathwayiUPA00378.
BioCyciYEAST:G3O-32212-MONOMER.

Miscellaneous databases

NextBioi966870.
PROiQ12205.

Family and domain databases

Gene3Di1.50.10.50. 4 hits.
InterProiIPR001382. Glyco_hydro_47.
[Graphical view]
PfamiPF01532. Glyco_hydro_47. 1 hit.
[Graphical view]
PRINTSiPR00747. GLYHDRLASE47.
SUPFAMiSSF48225. SSF48225. 4 hits.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XII."
    Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H.
    , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D.
    Nature 387:87-90(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  5. "Mnl2, a novel component of the ER associated protein degradation pathway."
    Martinez Benitez E., Stolz A., Becher A., Wolf D.H.
    Biochem. Biophys. Res. Commun. 414:528-532(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, FUNCTION.

Entry informationi

Entry nameiMNL2_YEAST
AccessioniPrimary (citable) accession number: Q12205
Secondary accession number(s): D6VY59, Q7LGY1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 25, 2006
Last sequence update: November 1, 1996
Last modified: May 11, 2016
This is version 115 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 71 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  5. Yeast chromosome XII
    Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.