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Protein

Putative endoplasmic reticulum mannosidase MNL2

Gene

MNL2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Putative mannosidase involved in glycoprotein quality control since it is involved in the targeting of misfolded glycoproteins for ER-associated protein degradation (ERAD).1 Publication

Miscellaneous

Present with 71 molecules/cell in log phase SD medium.1 Publication

Cofactori

Ca2+By similarity

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.By similarity
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

GO - Molecular functioni

GO - Biological processi

  • N-glycan processing Source: GO_Central
  • protein glycosylation Source: UniProtKB-UniPathway
  • ubiquitin-dependent ERAD pathway Source: SGD

Keywordsi

Molecular functionGlycosidase, Hydrolase

Enzyme and pathway databases

BioCyciYEAST:G3O-32212-MONOMER
UniPathwayiUPA00378

Protein family/group databases

CAZyiGH47 Glycoside Hydrolase Family 47

Names & Taxonomyi

Protein namesi
Recommended name:
Putative endoplasmic reticulum mannosidase MNL2 (EC:3.2.1.-By similarity)
Alternative name(s):
Mannosidase-like protein 2
Gene namesi
Name:MNL2
Ordered Locus Names:YLR057W
ORF Names:L2153
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XII

Organism-specific databases

EuPathDBiFungiDB:YLR057W
SGDiS000004047 MNL2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 12CytoplasmicSequence analysisAdd BLAST12
Transmembranei13 – 32Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST20
Topological domaini33 – 849LumenalSequence analysisAdd BLAST817

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002472291 – 849Putative endoplasmic reticulum mannosidase MNL2Add BLAST849

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi45N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi559 ↔ 598By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

MaxQBiQ12205
PaxDbiQ12205
PRIDEiQ12205

PTM databases

iPTMnetiQ12205

Interactioni

Protein-protein interaction databases

BioGridi31332, 66 interactors
DIPiDIP-8921N
MINTiQ12205
STRINGi4932.YLR057W

Structurei

3D structure databases

ProteinModelPortaliQ12205
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 47 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

HOGENOMiHOG000142372
InParanoidiQ12205
KOiK01230
OMAiEKNKYFP
OrthoDBiEOG092C1PJZ

Family and domain databases

Gene3Di1.50.10.10, 2 hits
InterProiView protein in InterPro
IPR012341 6hp_glycosidase-like_sf
IPR001382 Glyco_hydro_47
IPR036026 Seven-hairpin_glycosidases
PfamiView protein in Pfam
PF01532 Glyco_hydro_47, 1 hit
PRINTSiPR00747 GLYHDRLASE47
SUPFAMiSSF48225 SSF48225, 4 hits

Sequencei

Sequence statusi: Complete.

Q12205-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSIARLVYSL FRRVRSVLLL FITISLLFYY TFQNEIDILN SYALNDSLPS
60 70 80 90 100
INNYEHNTEG SSKLDPPDLS STGSDRIATD KENGNVAVDL SDPATLREKN
110 120 130 140 150
KYFPLLLKGS SHQIGSNLPI SSLLTYKEKY PVLFEYSSPS LTSISQNDVH
160 170 180 190 200
KIQPAMQLPP DVDMIKQIKD IFMKSWNQEQ LLLKSNLRRE STWPIDLIDS
210 220 230 240 250
LDTLYLCGET KLFQDSVNII EDFDFRVPPL AMEVIDIPDI TTRVLEGLLS
260 270 280 290 300
AYELSMDKRL LNKAKHVADF ILRSFDTPNR IPILKYFWKS DLRNRFPDRT
310 320 330 340 350
VPSGQLTTMA LAFIRLSQLT RLNKYFDAVE RVFTTIRQSY NEFDMEFMLP
360 370 380 390 400
DVVDASGCQL LTQEEIENGA HLKGSSIMKS INENFKFVHC QQLGKFLNPP
410 420 430 440 450
IDDNSLQEQS QYQAYRINEK TVPILENLFK INDLFQSSYD ILDGSSKNAN
460 470 480 490 500
AATMDPSIGS EVEAVDEIIE KRNFKDGTKK DSTKNTVGDK SLIDSQTFLT
510 520 530 540 550
NSISNIFKFM TFRPMLPKQT ENKKFNFLNS ILTKSQFMPT TNELDVTIRK
560 570 580 590 600
SYDVSLYSCR LGGILGLSSR VPHRGGVNTK YILPSSLLEM SEIITESCFM
610 620 630 640 650
LMEEFDGLLP QKFELDPCTD ETNGNCEFNG ETKSRMIANG EYETFENDLD
660 670 680 690 700
VGIKVSNYGK GGNDQKAKRN VLSKDGITET QNIKGDTVGS SKSIAEIDGD
710 720 730 740 750
EVTQIRRVFT LGKDIKPHIT TDDTMGSQWK NHPDWPFWVN KVESRRLLDS
760 770 780 790 800
NIIESIFYMY RISGEQKWRS MGKQSFGILM QELMELNSGA KGLWQIKEFY
810 820 830 840
ENGEKVNNDL PSYWFSRTLK YYLLLFSDGD KVSLDKHILT QGGHIIKKK
Length:849
Mass (Da):96,997
Last modified:November 1, 1996 - v1
Checksum:iA6B87AC32936A0D5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X94607 Genomic DNA Translation: CAA64304.1
Z73229 Genomic DNA Translation: CAA97587.1
Z73230 Genomic DNA Translation: CAA97589.1
AY692557 Genomic DNA Translation: AAT92576.1
BK006945 Genomic DNA Translation: DAA09375.1
PIRiS61631
RefSeqiNP_013158.1, NM_001181944.1

Genome annotation databases

EnsemblFungiiYLR057W; YLR057W; YLR057W
GeneIDi850746
KEGGisce:YLR057W

Similar proteinsi

Entry informationi

Entry nameiMNL2_YEAST
AccessioniPrimary (citable) accession number: Q12205
Secondary accession number(s): D6VY59, Q7LGY1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 25, 2006
Last sequence update: November 1, 1996
Last modified: May 23, 2018
This is version 133 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  5. Yeast chromosome XII
    Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

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