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Protein

Niemann-Pick type C-related protein 1

Gene

NCR1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Involved in sphingolipid trafficking. May recycle sphingolipids between cellular membranous compartments.2 Publications

GO - Molecular functioni

  • sphingolipid transporter activity Source: SGD

GO - Biological processi

  • sphingolipid metabolic process Source: SGD
Complete GO annotation...

Keywords - Biological processi

Lipid metabolism, Lipid transport, Sphingolipid metabolism, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-33925-MONOMER.
ReactomeiR-SCE-265473. Trafficking of dietary sterols.
R-SCE-5632684. Hedgehog 'on' state.

Protein family/group databases

TCDBi2.A.6.6.3. the resistance-nodulation-cell division (rnd) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
Niemann-Pick type C-related protein 1
Gene namesi
Name:NCR1
Ordered Locus Names:YPL006W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XVI

Organism-specific databases

EuPathDBiFungiDB:YPL006W.
SGDiS000005927. NCR1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei260 – 28021HelicalSequence analysisAdd
BLAST
Transmembranei341 – 36121HelicalSequence analysisAdd
BLAST
Transmembranei556 – 57621HelicalSequence analysisAdd
BLAST
Transmembranei585 – 60521HelicalSequence analysisAdd
BLAST
Transmembranei616 – 63621HelicalSequence analysisAdd
BLAST
Transmembranei667 – 68721HelicalSequence analysisAdd
BLAST
Transmembranei698 – 71821HelicalSequence analysisAdd
BLAST
Transmembranei752 – 77221HelicalSequence analysisAdd
BLAST
Transmembranei1000 – 102021HelicalSequence analysisAdd
BLAST
Transmembranei1027 – 104721HelicalSequence analysisAdd
BLAST
Transmembranei1054 – 107421HelicalSequence analysisAdd
BLAST
Transmembranei1099 – 111921HelicalSequence analysisAdd
BLAST
Transmembranei1133 – 115321HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

  • fungal-type vacuole membrane Source: SGD
  • integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Vacuole

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi718 – 7181Y → D: Sphingolipids mislocalization and no growth at 38 degrees Celsius. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1919Sequence analysisAdd
BLAST
Chaini20 – 11701151Niemann-Pick type C-related protein 1PRO_0000268685Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi123 – 1231N-linked (GlcNAc...)Sequence analysis
Glycosylationi145 – 1451N-linked (GlcNAc...)Sequence analysis
Glycosylationi178 – 1781N-linked (GlcNAc...)Sequence analysis
Glycosylationi314 – 3141N-linked (GlcNAc...)Sequence analysis
Glycosylationi401 – 4011N-linked (GlcNAc...)Sequence analysis
Glycosylationi513 – 5131N-linked (GlcNAc...)Sequence analysis
Glycosylationi900 – 9001N-linked (GlcNAc...)Sequence analysis
Glycosylationi940 – 9401N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiQ12200.

Interactioni

Protein-protein interaction databases

BioGridi36171. 98 interactions.
IntActiQ12200. 5 interactions.
MINTiMINT-1552235.

Structurei

3D structure databases

ProteinModelPortaliQ12200.
SMRiQ12200. Positions 23-240, 1002-1152.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini557 – 717161SSDPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the patched family.Curated
Contains 1 SSD (sterol-sensing) domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00800000124043.
HOGENOMiHOG000036674.
InParanoidiQ12200.
KOiK12385.
OMAiVHALCLL.
OrthoDBiEOG72VHFB.

Family and domain databases

InterProiIPR004765. NP_C_type.
IPR032190. NPC1_N.
IPR003392. Ptc/Disp.
IPR000731. SSD.
[Graphical view]
PANTHERiPTHR10796:SF116. PTHR10796:SF116. 1 hit.
PfamiPF16414. NPC1_N. 1 hit.
PF02460. Patched. 1 hit.
[Graphical view]
PROSITEiPS50156. SSD. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q12200-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNVLWIIALV GQLMRLVQGT ATCAMYGNCG KKSVFGNELP CPVPRSFEPP
60 70 80 90 100
VLSDETSKLL VEVCGEEWKE VRYACCTKDQ VVALRDNLQK AQPLISSCPA
110 120 130 140 150
CLKNFNNLFC HFTCAADQGR FVNITKVEKS KEDKDIVAEL DVFMNSSWAS
160 170 180 190 200
EFYDSCKNIK FSATNGYAMD LIGGGAKNYS QFLKFLGDAK PMLGGSPFQI
210 220 230 240 250
NYKYDLANEE KEWQEFNDEV YACDDAQYKC ACSDCQESCP HLKPLKDGVC
260 270 280 290 300
KVGPLPCFSL SVLIFYTICA LFAFMWYYLC KRKKNGAMIV DDDIVPESGS
310 320 330 340 350
LDESETNVFE SFNNETNFFN GKLANLFTKV GQFSVENPYK ILITTVFSIF
360 370 380 390 400
VFSFIIFQYA TLETDPINLW VSKNSEKFKE KEYFDDNFGP FYRTEQIFVV
410 420 430 440 450
NETGPVLSYE TLHWWFDVEN FITEELQSSE NIGYQDLCFR PTEDSTCVIE
460 470 480 490 500
SFTQYFQGAL PNKDSWKREL QECGKFPVNC LPTFQQPLKT NLLFSDDDIL
510 520 530 540 550
NAHAFVVTLL LTNHTQSANR WEERLEEYLL DLKVPEGLRI SFNTEISLEK
560 570 580 590 600
ELNNNNDIST VAISYLMMFL YATWALRRKD GKTRLLLGIS GLLIVLASIV
610 620 630 640 650
CAAGFLTLFG LKSTLIIAEV IPFLILAIGI DNIFLITHEY DRNCEQKPEY
660 670 680 690 700
SIDQKIISAI GRMSPSILMS LLCQTGCFLI AAFVTMPAVH NFAIYSTVSV
710 720 730 740 750
IFNGVLQLTA YVSILSLYEK RSNYKQITGN EETKESFLKT FYFKMLTQKR
760 770 780 790 800
LIIIIFSAWF FTSLVFLPEI QFGLDQTLAV PQDSYLVDYF KDVYSFLNVG
810 820 830 840 850
PPVYMVVKNL DLTKRQNQQK ICGKFTTCER DSLANVLEQE RHRSTITEPL
860 870 880 890 900
ANWLDDYFMF LNPQNDQCCR LKKGTDEVCP PSFPSRRCET CFQQGSWNYN
910 920 930 940 950
MSGFPEGKDF MEYLSIWINA PSDPCPLGGR APYSTALVYN ETSVSASVFR
960 970 980 990 1000
TAHHPLRSQK DFIQAYSDGV RISSSFPELD MFAYSPFYIF FVQYQTLGPL
1010 1020 1030 1040 1050
TLKLIGSAII LIFFISSVFL QNIRSSFLLA LVVTMIIVDI GALMALLGIS
1060 1070 1080 1090 1100
LNAVSLVNLI ICVGLGVEFC VHIVRSFTVV PSETKKDANS RVLYSLNTIG
1110 1120 1130 1140 1150
ESVIKGITLT KFIGVCVLAF AQSKIFDVFY FRMWFTLIIV AALHALLFLP
1160 1170
ALLSLFGGES YRDDSIEAED
Length:1,170
Mass (Da):132,645
Last modified:November 1, 1996 - v1
Checksum:iF976EBDB36C36C92
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U33335 Genomic DNA. Translation: AAB68099.1.
Z48483 Genomic DNA. Translation: CAA88380.1.
Z71255 Genomic DNA. Translation: CAA95035.1.
BK006949 Genomic DNA. Translation: DAA11422.1.
PIRiS52525.
RefSeqiNP_015319.1. NM_001183820.1.

Genome annotation databases

EnsemblFungiiYPL006W; YPL006W; YPL006W.
GeneIDi856101.
KEGGisce:YPL006W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U33335 Genomic DNA. Translation: AAB68099.1.
Z48483 Genomic DNA. Translation: CAA88380.1.
Z71255 Genomic DNA. Translation: CAA95035.1.
BK006949 Genomic DNA. Translation: DAA11422.1.
PIRiS52525.
RefSeqiNP_015319.1. NM_001183820.1.

3D structure databases

ProteinModelPortaliQ12200.
SMRiQ12200. Positions 23-240, 1002-1152.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36171. 98 interactions.
IntActiQ12200. 5 interactions.
MINTiMINT-1552235.

Protein family/group databases

TCDBi2.A.6.6.3. the resistance-nodulation-cell division (rnd) superfamily.

Proteomic databases

MaxQBiQ12200.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYPL006W; YPL006W; YPL006W.
GeneIDi856101.
KEGGisce:YPL006W.

Organism-specific databases

EuPathDBiFungiDB:YPL006W.
SGDiS000005927. NCR1.

Phylogenomic databases

GeneTreeiENSGT00800000124043.
HOGENOMiHOG000036674.
InParanoidiQ12200.
KOiK12385.
OMAiVHALCLL.
OrthoDBiEOG72VHFB.

Enzyme and pathway databases

BioCyciYEAST:G3O-33925-MONOMER.
ReactomeiR-SCE-265473. Trafficking of dietary sterols.
R-SCE-5632684. Hedgehog 'on' state.

Miscellaneous databases

PROiQ12200.

Family and domain databases

InterProiIPR004765. NP_C_type.
IPR032190. NPC1_N.
IPR003392. Ptc/Disp.
IPR000731. SSD.
[Graphical view]
PANTHERiPTHR10796:SF116. PTHR10796:SF116. 1 hit.
PfamiPF16414. NPC1_N. 1 hit.
PF02460. Patched. 1 hit.
[Graphical view]
PROSITEiPS50156. SSD. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI."
    Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M.
    , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J.
    Nature 387:103-105(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  4. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  5. "Mutagenesis of the putative sterol-sensing domain of yeast Niemann Pick C-related protein reveals a primordial role in subcellular sphingolipid distribution."
    Malathi K., Higaki K., Tinkelenberg A.H., Balderes D.A., Almanzar-Paramio D., Wilcox L.J., Erdeniz N., Redican F., Padamsee M., Liu Y., Khan S., Alcantara F., Carstea E.D., Morris J.A., Sturley S.L.
    J. Cell Biol. 164:547-556(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, MUTAGENESIS OF TYR-718.
  6. "Ncr1p, the yeast ortholog of mammalian Niemann Pick C1 protein, is dispensable for endocytic transport."
    Zhang S., Ren J., Li H., Zhang Q., Armstrong J.S., Munn A.L., Yang H.
    Traffic 5:1017-1030(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
  7. "A yeast model system for functional analysis of the Niemann-Pick type C protein 1 homolog, Ncr1p."
    Berger A.C., Hanson P.K., Wylie Nichols J., Corbett A.H.
    Traffic 6:907-917(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiNPC1_YEAST
AccessioniPrimary (citable) accession number: Q12200
Secondary accession number(s): D6W406
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 12, 2006
Last sequence update: November 1, 1996
Last modified: July 6, 2016
This is version 121 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 521 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XVI
    Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.