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Protein

Putative cystathionine gamma-synthase YLL058W

Gene

YLL058W

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the formation of L-cystathionine from O-succinyl-L-homoserine (OSHS) and L-cysteine, via a gamma-replacement reaction. In the absence of thiol, catalyzes gamma-elimination to form 2-oxobutanoate, succinate and ammonia (By similarity).By similarity

Catalytic activityi

O(4)-succinyl-L-homoserine + L-cysteine = L-cystathionine + succinate.

Cofactori

Pathwayi: L-methionine biosynthesis via de novo pathway

This protein is involved in step 1 of the subpathway that synthesizes L-cystathionine from O-succinyl-L-homoserine.
Proteins known to be involved in this subpathway in this organism are:
  1. Putative cystathionine gamma-synthase YML082W (YML082W), Cystathionine gamma-synthase (STR2), Putative cystathionine gamma-synthase YLL058W (YLL058W)
This subpathway is part of the pathway L-methionine biosynthesis via de novo pathway, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-cystathionine from O-succinyl-L-homoserine, the pathway L-methionine biosynthesis via de novo pathway and in Amino-acid biosynthesis.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Amino-acid biosynthesis, Methionine biosynthesis

Keywords - Ligandi

Pyridoxal phosphate

Enzyme and pathway databases

BioCyciYEAST:G3O-32157-MONOMER.
UniPathwayiUPA00051; UER00077.

Names & Taxonomyi

Protein namesi
Recommended name:
Putative cystathionine gamma-synthase YLL058W (EC:2.5.1.48)
Alternative name(s):
O-succinylhomoserine (thiol)-lyase
Gene namesi
Ordered Locus Names:YLL058W
ORF Names:L0569
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XII

Organism-specific databases

EuPathDBiFungiDB:YLL058W.
SGDiS000003981. YLL058W.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 575575Putative cystathionine gamma-synthase YLL058WPRO_0000114782Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei376 – 3761N6-(pyridoxal phosphate)lysineSequence analysis

Proteomic databases

MaxQBiQ12198.

Interactioni

Protein-protein interaction databases

BioGridi31257. 19 interactions.

Structurei

3D structure databases

ProteinModelPortaliQ12198.
SMRiQ12198. Positions 206-565.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00390000016853.
HOGENOMiHOG000193384.
InParanoidiQ12198.
KOiK01739.
OrthoDBiEOG092C18EA.

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
InterProiIPR000277. Cys/Met-Metab_PyrdxlP-dep_enz.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]
PANTHERiPTHR11808. PTHR11808. 2 hits.
PfamiPF01053. Cys_Met_Meta_PP. 1 hit.
[Graphical view]
SUPFAMiSSF53383. SSF53383. 1 hit.

Sequencei

Sequence statusi: Complete.

Q12198-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTEIEFGQPL PSNLDYAVSF GIPTWDSAIG YAEKVPEVIG KMATGYPRYF
60 70 80 90 100
PQPPVQRLCA YFVKKFGRGS ENCRPFPSVN LGLKCFEYVK SVSGPESKAH
110 120 130 140 150
LEVETVTIKN RGAKTSKEPA ELVLTIAAVL ASEEEFETVK EYWKLRGECV
160 170 180 190 200
SSRLALSVNQ LLDCANHGSE QVLRELEAGV FAAKKGEEKA KNLIKGRIVE
210 220 230 240 250
NRFRPFGLEK KTPNWEGLNL NPNEDVYLVS SGMSAISTAR NLLTFWEEKK
260 270 280 290 300
NSGDSLNKTT SDQKKKPLLC DTVGIFGFPF KDTQVIMTKF GKCKFFGFGN
310 320 330 340 350
SRDVVELQKF LETSKQRILA VFVETPSNPL LNMPDLKKLR SLADQYGFFI
360 370 380 390 400
VIDDTIGGLN VDILPYADIV STSLTKLFNG ASNVMGGSVV LNPKSSLYPY
410 420 430 440 450
AREYFRSANF EDLLWCEDAI VLERNSRDFE DRTLRANANT GILLNDLLLP
460 470 480 490 500
EEGKICKKIY YPTVTSKETF ENYESVRNER GGYGCLFSVA FFNEGDAKAF
510 520 530 540 550
YDSLKVFKGP SNGTNFTLAC PYVHLAHHSE LEEVSKFGAD PNIIRVSVGL
560 570
EDIQWLLKVF SSALDVVKSR GSKHS
Length:575
Mass (Da):64,223
Last modified:November 1, 1996 - v1
Checksum:iEDE6292C60ADFDC1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z47973 Genomic DNA. Translation: CAA87999.1.
Z73163 Genomic DNA. Translation: CAA97511.1.
BK006945 Genomic DNA. Translation: DAA09266.1.
PIRiS50962.
RefSeqiNP_013042.1. NM_001181878.1.

Genome annotation databases

EnsemblFungiiYLL058W; YLL058W; YLL058W.
GeneIDi850668.
KEGGisce:YLL058W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z47973 Genomic DNA. Translation: CAA87999.1.
Z73163 Genomic DNA. Translation: CAA97511.1.
BK006945 Genomic DNA. Translation: DAA09266.1.
PIRiS50962.
RefSeqiNP_013042.1. NM_001181878.1.

3D structure databases

ProteinModelPortaliQ12198.
SMRiQ12198. Positions 206-565.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi31257. 19 interactions.

Proteomic databases

MaxQBiQ12198.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYLL058W; YLL058W; YLL058W.
GeneIDi850668.
KEGGisce:YLL058W.

Organism-specific databases

EuPathDBiFungiDB:YLL058W.
SGDiS000003981. YLL058W.

Phylogenomic databases

GeneTreeiENSGT00390000016853.
HOGENOMiHOG000193384.
InParanoidiQ12198.
KOiK01739.
OrthoDBiEOG092C18EA.

Enzyme and pathway databases

UniPathwayiUPA00051; UER00077.
BioCyciYEAST:G3O-32157-MONOMER.

Miscellaneous databases

PROiQ12198.

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
InterProiIPR000277. Cys/Met-Metab_PyrdxlP-dep_enz.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]
PANTHERiPTHR11808. PTHR11808. 2 hits.
PfamiPF01053. Cys_Met_Meta_PP. 1 hit.
[Graphical view]
SUPFAMiSSF53383. SSF53383. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiMETW_YEAST
AccessioniPrimary (citable) accession number: Q12198
Secondary accession number(s): D6VXV0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 23, 2003
Last sequence update: November 1, 1996
Last modified: September 7, 2016
This is version 114 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 2070 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XII
    Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.