Q12198 (METW_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Putative cystathionine gamma-synthase YLL058W
EC=2.5.1.48

Alternative name(s):
O-succinylhomoserine (thiol)-lyase

Gene names

Ordered Locus Names:	YLL058W
ORF Names:	L0569

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	575 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	O(4)-succinyl-L-homoserine + L-cysteine = L-cystathionine + succinate.
Cofactor	Pyridoxal phosphate.
Pathway	Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-cystathionine from O-succinyl-L-homoserine: step 1/1.
Miscellaneous	Present with 2070 molecules/cell in log phase SD medium. Ref.4
Sequence similarities	Belongs to the trans-sulfuration enzymes family. MET7 subfamily.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Methionine biosynthesis
Ligand	Pyridoxal phosphate
Molecular function	Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	methionine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	cystathionine gamma-synthase activity Inferred from electronic annotation. Source: EC pyridoxal phosphate binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
YHR080C	P38800	1	EBI-2101750,EBI-24597

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 575	575	Putative cystathionine gamma-synthase YLL058W		PRO_0000114782
Amino acid modifications
	Modified residue	376	1	N6-(pyridoxal phosphate)lysine Potential

Sequences

	Sequence		Length	Mass (Da)
	Q12198 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: EDE6292C60ADFDC1	FASTA	575	64,223
	10 20 30 40 50 60 MTEIEFGQPL PSNLDYAVSF GIPTWDSAIG YAEKVPEVIG KMATGYPRYF PQPPVQRLCA 70 80 90 100 110 120 YFVKKFGRGS ENCRPFPSVN LGLKCFEYVK SVSGPESKAH LEVETVTIKN RGAKTSKEPA 130 140 150 160 170 180 ELVLTIAAVL ASEEEFETVK EYWKLRGECV SSRLALSVNQ LLDCANHGSE QVLRELEAGV 190 200 210 220 230 240 FAAKKGEEKA KNLIKGRIVE NRFRPFGLEK KTPNWEGLNL NPNEDVYLVS SGMSAISTAR 250 260 270 280 290 300 NLLTFWEEKK NSGDSLNKTT SDQKKKPLLC DTVGIFGFPF KDTQVIMTKF GKCKFFGFGN 310 320 330 340 350 360 SRDVVELQKF LETSKQRILA VFVETPSNPL LNMPDLKKLR SLADQYGFFI VIDDTIGGLN 370 380 390 400 410 420 VDILPYADIV STSLTKLFNG ASNVMGGSVV LNPKSSLYPY AREYFRSANF EDLLWCEDAI 430 440 450 460 470 480 VLERNSRDFE DRTLRANANT GILLNDLLLP EEGKICKKIY YPTVTSKETF ENYESVRNER 490 500 510 520 530 540 GGYGCLFSVA FFNEGDAKAF YDSLKVFKGP SNGTNFTLAC PYVHLAHHSE LEEVSKFGAD 550 560 570 PNIIRVSVGL EDIQWLLKVF SSALDVVKSR GSKHS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence of a 37 kb DNA fragment from chromosome XII of Saccharomyces cerevisiae including the subtelomeric region of the left arm." Wedler H., Wambutt R. Submitted (JAN-1995) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XII." Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H. , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D. Nature 387:87-90(1997) [PubMed: 9169871] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z47973 Genomic DNA. Translation: CAA87999.1. Z73163 Genomic DNA. Translation: CAA97511.1. BK006945 Genomic DNA. Translation: DAA09266.1.
PIR	S50962.
RefSeq	NP_013042.1. NM_001181878.1.
3D structure databases
ProteinModelPortal	Q12198.
SMR	Q12198. Positions 188-567.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q12198. 1 interaction.
STRING	Q12198.
Proteomic databases
PeptideAtlas	Q12198.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YLL058W; YLL058W; YLL058W.
GeneID	850668.
KEGG	sce:YLL058W.
NMPDR	fig|4932.3.peg.4033.
Organism-specific databases
CYGD	YLL058w.
SGD	S000003981. YLL058W.
Phylogenomic databases
eggNOG	fuNOG05525.
GeneTree	EFGT00050000003427.
HOGENOM	HBG634482.
OrthoDB	EOG47DDS5.
Gene expression databases
ArrayExpress	Q12198.
Genevestigator	Q12198.
GermOnline	YLL058W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR000277. Cys/Met-Metab_PyrdxlP-dep_enz. IPR015424. PyrdxlP-dep_Trfase_major_dom. IPR015421. PyrdxlP-dep_Trfase_major_sub1. IPR015422. PyrdxlP-dep_Trfase_major_sub2. [Graphical view]
Gene3D	G3DSA:3.40.640.10. PyrdxlP-dep_Trfase_major_sub1. 1 hit. G3DSA:3.90.1150.10. PyrdxlP-dep_Trfase_major_sub2. 1 hit.
KO	K01739.
PANTHER	PTHR11808. Cys_Met_Meta_PP. 1 hit.
Pfam	PF01053. Cys_Met_Meta_PP. 1 hit. [Graphical view]
SUPFAM	SSF53383. PyrdxlP-dep_Trfase_major. 1 hit.
ProtoNet	Search...
Other
NextBio	966647.

Entry information

Entry name

METW_YEAST

Accession

Primary (citable) accession number: Q12198
Secondary accession number(s): D6VXV0

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 23, 2003
Last sequence update:	November 1, 1996
Last modified:	December 14, 2011
This is version 78 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program