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Protein

Branchpoint-bridging protein

Gene

MSL5

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Required for pre-spliceosome formation, which is the first step of pre-mRNA splicing. 2 commitment complexes, CC1 and CC2, have been defined. CC1 is a basal complex dependent only on the 5'-splice site. CC2 is a complex of lower mobility and is dependent on a branchpoint as well as a 5'-splice site region. This protein is involved in CC2 formation where it binds to the snRNP U1-associated protein PRP40, bridging the U1 snRNP-associated 5'-splice site and the MSL5-associated branch point 3' intron splice site. Involved in nuclear retention of pre-mRNA.3 Publications

Miscellaneous

Present with 4380 molecules/cell in log phase SD medium.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri271 – 288CCHC-type 1PROSITE-ProRule annotationAdd BLAST18
Zinc fingeri297 – 314CCHC-type 2PROSITE-ProRule annotationAdd BLAST18

GO - Molecular functioni

  • pre-mRNA branch point binding Source: SGD
  • zinc ion binding Source: InterPro

GO - Biological processi

  • mRNA splicing, via spliceosome Source: SGD

Keywordsi

Molecular functionRNA-binding
Biological processmRNA processing, mRNA splicing
LigandMetal-binding, Zinc

Enzyme and pathway databases

BioCyciYEAST:G3O-32261-MONOMER
ReactomeiR-SCE-8849468 PTK6 Regulates Proteins Involved in RNA Processing

Names & Taxonomyi

Protein namesi
Recommended name:
Branchpoint-bridging protein
Alternative name(s):
Mud synthetic-lethal 5 protein
Splicing factor 1
Zinc finger protein BBP
Gene namesi
Name:MSL5
Synonyms:BBP, SF1
Ordered Locus Names:YLR116W
ORF Names:L2949
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XII

Organism-specific databases

EuPathDBiFungiDB:YLR116W
SGDiS000004106 MSL5

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi60R → G in MSL5-2; temperature-sensitive; growth defect; when associated with N-72 and S-155. 1 Publication1
Mutagenesisi72I → N in MSL5-2; temperature-sensitive; growth defect; when associated with G-60 and S-155. 1 Publication1
Mutagenesisi155P → S in MSL5-2; temperature-sensitive; growth defect; when associated with G-60 and N-72. 1 Publication1
Mutagenesisi195V → D in MSL5-5; cold-sensitive; growth defect; when associated with V-258. 1 Publication1
Mutagenesisi230G → S in msl-5; loss of function. 1 Publication1
Mutagenesisi258E → V in MSL5-5; cold-sensitive; growth defect; when associated with D-195. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002345641 – 476Branchpoint-bridging proteinAdd BLAST476

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei93PhosphoserineCombined sources1
Modified residuei95PhosphoserineCombined sources1
Modified residuei100PhosphotyrosineCombined sources1
Modified residuei382PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ12186
PaxDbiQ12186
PRIDEiQ12186

PTM databases

iPTMnetiQ12186

Interactioni

Subunit structurei

Interacts with MUD2 and PRP40. The proline-rich sequence of this protein interacts with the GYF domains of SMY2 and SYH1.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
MUD2P360847EBI-34012,EBI-11612

Protein-protein interaction databases

BioGridi31388, 261 interactors
DIPiDIP-991N
IntActiQ12186, 9 interactors
MINTiQ12186
STRINGi4932.YLR116W

Structurei

Secondary structure

1476
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi148 – 153Combined sources6
Turni156 – 158Combined sources3
Helixi164 – 169Combined sources6
Helixi171 – 173Combined sources3
Helixi174 – 183Combined sources10
Beta strandi186 – 192Combined sources7
Helixi201 – 203Combined sources3
Turni206 – 209Combined sources4
Beta strandi212 – 223Combined sources12
Helixi224 – 242Combined sources19
Helixi250 – 262Combined sources13

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3FMAX-ray2.50L/M/N/O/P440-450[»]
4WALX-ray2.20A144-271[»]
4WANX-ray1.80A/C/E/G144-271[»]
ProteinModelPortaliQ12186
SMRiQ12186
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ12186

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini153 – 219KHAdd BLAST67

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi363 – 474Pro-richAdd BLAST112

Sequence similaritiesi

Belongs to the BBP/SF1 family.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri271 – 288CCHC-type 1PROSITE-ProRule annotationAdd BLAST18
Zinc fingeri297 – 314CCHC-type 2PROSITE-ProRule annotationAdd BLAST18

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

GeneTreeiENSGT00550000074434
HOGENOMiHOG000197271
InParanoidiQ12186
KOiK13095
OMAiQYFRIEE
OrthoDBiEOG092C2J4N

Family and domain databases

Gene3Di3.30.1370.10, 1 hit
InterProiView protein in InterPro
IPR031150 BBP/SF1
IPR013041 Clathrin_app_Ig-like_sf
IPR004087 KH_dom
IPR004088 KH_dom_type_1
IPR036612 KH_dom_type_1_sf
IPR032570 SF1-HH
IPR001878 Znf_CCHC
IPR036875 Znf_CCHC_sf
PANTHERiPTHR11208:SF45 PTHR11208:SF45, 1 hit
PfamiView protein in Pfam
PF00013 KH_1, 1 hit
PF16275 SF1-HH, 1 hit
PF00098 zf-CCHC, 1 hit
SMARTiView protein in SMART
SM00322 KH, 1 hit
SM00343 ZnF_C2HC, 2 hits
SUPFAMiSSF49348 SSF49348, 1 hit
SSF54791 SSF54791, 1 hit
SSF57756 SSF57756, 1 hit
PROSITEiView protein in PROSITE
PS50158 ZF_CCHC, 1 hit

Sequencei

Sequence statusi: Complete.

Q12186-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSFRRINSRY FENRKGSSME EKKAKVPPNV NLSLWRKNTV ESDVHRFNSL
60 70 80 90 100
PSKISGALTR EQIYSYQVMF RIQEITIKLR TNDFVPPSRK NRSPSPPPVY
110 120 130 140 150
DAQGKRTNTR EQRYRKKLED ERIKLVEIAL KTIPYFVPPD DYKRPTKFQD
160 170 180 190 200
KYYIPVDQYP DVNFVGLLLG PRGRTLRKLQ EDSNCKIAIR GRGSVKEGKN
210 220 230 240 250
ASDLPPGAMN FEDPLHCLII ADSEDKIQKG IKVCQNIVIK AVTSPEGQND
260 270 280 290 300
LKRGQLRELA ELNGTLREDN RPCPICGLKD HKRYDCPNRK IPNIQGIVCK
310 320 330 340 350
ICGQTGHFSR DCNSSSQRMS RFDRNATVNN SAPIQSNDVH YNSNTHPIQA
360 370 380 390 400
PKRSRYDNNS TEPPLKFPAS SRYAPSPSPP ASHISRQAQN VTPTPPPGLT
410 420 430 440 450
SSSFSSGVPG IAPPPLQSPP ESEQPKFSLP PPPGMTTVQS SIAPPPGLSG
460 470
PPGFSNNMGN DINKPTPPGL QGPPGL
Length:476
Mass (Da):53,034
Last modified:November 1, 1996 - v1
Checksum:iF94FF7175345C47D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X89514 Genomic DNA Translation: CAA61695.1
U53877 Genomic DNA Translation: AAB82363.1
Z73288 Genomic DNA Translation: CAA97683.1
BK006945 Genomic DNA Translation: DAA09430.1
PIRiS64953
RefSeqiNP_013217.1, NM_001182003.1

Genome annotation databases

EnsemblFungiiYLR116W; YLR116W; YLR116W
GeneIDi850807
KEGGisce:YLR116W

Similar proteinsi

Entry informationi

Entry nameiBBP_YEAST
AccessioniPrimary (citable) accession number: Q12186
Secondary accession number(s): D6VYB4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 16, 2006
Last sequence update: November 1, 1996
Last modified: March 28, 2018
This is version 142 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health