Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Branchpoint-bridging protein

Gene

MSL5

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Required for pre-spliceosome formation, which is the first step of pre-mRNA splicing. 2 commitment complexes, CC1 and CC2, have been defined. CC1 is a basal complex dependent only on the 5'-splice site. CC2 is a complex of lower mobility and is dependent on a branchpoint as well as a 5'-splice site region. This protein is involved in CC2 formation where it binds to the snRNP U1-associated protein PRP40, bridging the U1 snRNP-associated 5'-splice site and the MSL5-associated branch point 3' intron splice site. Involved in nuclear retention of pre-mRNA.3 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri271 – 288CCHC-type 1PROSITE-ProRule annotationAdd BLAST18
Zinc fingeri297 – 314CCHC-type 2PROSITE-ProRule annotationAdd BLAST18

GO - Molecular functioni

  • pre-mRNA branch point binding Source: SGD
  • zinc ion binding Source: InterPro

GO - Biological processi

  • mRNA splicing, via spliceosome Source: SGD
Complete GO annotation...

Keywords - Biological processi

mRNA processing, mRNA splicing

Keywords - Ligandi

Metal-binding, RNA-binding, Zinc

Enzyme and pathway databases

BioCyciYEAST:G3O-32261-MONOMER.
ReactomeiR-SCE-8849468. PTK6 Regulates Proteins Involved in RNA Processing.

Names & Taxonomyi

Protein namesi
Recommended name:
Branchpoint-bridging protein
Alternative name(s):
Mud synthetic-lethal 5 protein
Splicing factor 1
Zinc finger protein BBP
Gene namesi
Name:MSL5
Synonyms:BBP, SF1
Ordered Locus Names:YLR116W
ORF Names:L2949
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XII

Organism-specific databases

EuPathDBiFungiDB:YLR116W.
SGDiS000004106. MSL5.

Subcellular locationi

GO - Cellular componenti

  • commitment complex Source: SGD
  • cytosol Source: SGD
  • nucleus Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi60R → G in MSL5-2; temperature-sensitive; growth defect; when associated with N-72 and S-155. 1 Publication1
Mutagenesisi72I → N in MSL5-2; temperature-sensitive; growth defect; when associated with G-60 and S-155. 1 Publication1
Mutagenesisi155P → S in MSL5-2; temperature-sensitive; growth defect; when associated with G-60 and N-72. 1 Publication1
Mutagenesisi195V → D in MSL5-5; cold-sensitive; growth defect; when associated with V-258. 1 Publication1
Mutagenesisi230G → S in msl-5; loss of function. 1 Publication1
Mutagenesisi258E → V in MSL5-5; cold-sensitive; growth defect; when associated with D-195. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002345641 – 476Branchpoint-bridging proteinAdd BLAST476

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei93PhosphoserineCombined sources1
Modified residuei95PhosphoserineCombined sources1
Modified residuei100PhosphotyrosineCombined sources1
Modified residuei382PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ12186.
PRIDEiQ12186.

PTM databases

iPTMnetiQ12186.

Interactioni

Subunit structurei

Interacts with MUD2 and PRP40. The proline-rich sequence of this protein interacts with the GYF domains of SMY2 and SYH1.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
MUD2P360845EBI-34012,EBI-11612

Protein-protein interaction databases

BioGridi31388. 72 interactors.
DIPiDIP-991N.
IntActiQ12186. 6 interactors.
MINTiMINT-489287.

Structurei

Secondary structure

1476
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi148 – 153Combined sources6
Turni156 – 158Combined sources3
Helixi164 – 169Combined sources6
Helixi171 – 173Combined sources3
Helixi174 – 183Combined sources10
Beta strandi186 – 192Combined sources7
Helixi201 – 203Combined sources3
Turni206 – 209Combined sources4
Beta strandi212 – 223Combined sources12
Helixi224 – 242Combined sources19
Helixi250 – 262Combined sources13

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3FMAX-ray2.50L/M/N/O/P440-450[»]
4WALX-ray2.20A144-271[»]
4WANX-ray1.80A/C/E/G144-271[»]
ProteinModelPortaliQ12186.
SMRiQ12186.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ12186.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini153 – 219KHAdd BLAST67

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi363 – 474Pro-richAdd BLAST112

Sequence similaritiesi

Belongs to the BBP/SF1 family.Curated
Contains 2 CCHC-type zinc fingers.PROSITE-ProRule annotation
Contains 1 KH domain.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri271 – 288CCHC-type 1PROSITE-ProRule annotationAdd BLAST18
Zinc fingeri297 – 314CCHC-type 2PROSITE-ProRule annotationAdd BLAST18

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

GeneTreeiENSGT00550000074434.
HOGENOMiHOG000197271.
InParanoidiQ12186.
KOiK13095.
OMAiQNIVIKA.
OrthoDBiEOG092C2J4N.

Family and domain databases

Gene3Di3.30.1370.10. 1 hit.
4.10.60.10. 1 hit.
InterProiIPR031150. BBP/SF1.
IPR013041. Coatomer/clathrin_app_Ig-like.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR032570. SF1-HH.
IPR001878. Znf_CCHC.
[Graphical view]
PANTHERiPTHR11208:SF45. PTHR11208:SF45. 1 hit.
PfamiPF00013. KH_1. 1 hit.
PF16275. SF1-HH. 1 hit.
PF00098. zf-CCHC. 1 hit.
[Graphical view]
SMARTiSM00322. KH. 1 hit.
SM00343. ZnF_C2HC. 2 hits.
[Graphical view]
SUPFAMiSSF49348. SSF49348. 1 hit.
SSF54791. SSF54791. 1 hit.
SSF57756. SSF57756. 1 hit.
PROSITEiPS50158. ZF_CCHC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q12186-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSFRRINSRY FENRKGSSME EKKAKVPPNV NLSLWRKNTV ESDVHRFNSL
60 70 80 90 100
PSKISGALTR EQIYSYQVMF RIQEITIKLR TNDFVPPSRK NRSPSPPPVY
110 120 130 140 150
DAQGKRTNTR EQRYRKKLED ERIKLVEIAL KTIPYFVPPD DYKRPTKFQD
160 170 180 190 200
KYYIPVDQYP DVNFVGLLLG PRGRTLRKLQ EDSNCKIAIR GRGSVKEGKN
210 220 230 240 250
ASDLPPGAMN FEDPLHCLII ADSEDKIQKG IKVCQNIVIK AVTSPEGQND
260 270 280 290 300
LKRGQLRELA ELNGTLREDN RPCPICGLKD HKRYDCPNRK IPNIQGIVCK
310 320 330 340 350
ICGQTGHFSR DCNSSSQRMS RFDRNATVNN SAPIQSNDVH YNSNTHPIQA
360 370 380 390 400
PKRSRYDNNS TEPPLKFPAS SRYAPSPSPP ASHISRQAQN VTPTPPPGLT
410 420 430 440 450
SSSFSSGVPG IAPPPLQSPP ESEQPKFSLP PPPGMTTVQS SIAPPPGLSG
460 470
PPGFSNNMGN DINKPTPPGL QGPPGL
Length:476
Mass (Da):53,034
Last modified:November 1, 1996 - v1
Checksum:iF94FF7175345C47D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X89514 Genomic DNA. Translation: CAA61695.1.
U53877 Genomic DNA. Translation: AAB82363.1.
Z73288 Genomic DNA. Translation: CAA97683.1.
BK006945 Genomic DNA. Translation: DAA09430.1.
PIRiS64953.
RefSeqiNP_013217.1. NM_001182003.1.

Genome annotation databases

EnsemblFungiiYLR116W; YLR116W; YLR116W.
GeneIDi850807.
KEGGisce:YLR116W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X89514 Genomic DNA. Translation: CAA61695.1.
U53877 Genomic DNA. Translation: AAB82363.1.
Z73288 Genomic DNA. Translation: CAA97683.1.
BK006945 Genomic DNA. Translation: DAA09430.1.
PIRiS64953.
RefSeqiNP_013217.1. NM_001182003.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3FMAX-ray2.50L/M/N/O/P440-450[»]
4WALX-ray2.20A144-271[»]
4WANX-ray1.80A/C/E/G144-271[»]
ProteinModelPortaliQ12186.
SMRiQ12186.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi31388. 72 interactors.
DIPiDIP-991N.
IntActiQ12186. 6 interactors.
MINTiMINT-489287.

PTM databases

iPTMnetiQ12186.

Proteomic databases

MaxQBiQ12186.
PRIDEiQ12186.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYLR116W; YLR116W; YLR116W.
GeneIDi850807.
KEGGisce:YLR116W.

Organism-specific databases

EuPathDBiFungiDB:YLR116W.
SGDiS000004106. MSL5.

Phylogenomic databases

GeneTreeiENSGT00550000074434.
HOGENOMiHOG000197271.
InParanoidiQ12186.
KOiK13095.
OMAiQNIVIKA.
OrthoDBiEOG092C2J4N.

Enzyme and pathway databases

BioCyciYEAST:G3O-32261-MONOMER.
ReactomeiR-SCE-8849468. PTK6 Regulates Proteins Involved in RNA Processing.

Miscellaneous databases

EvolutionaryTraceiQ12186.
PROiQ12186.

Family and domain databases

Gene3Di3.30.1370.10. 1 hit.
4.10.60.10. 1 hit.
InterProiIPR031150. BBP/SF1.
IPR013041. Coatomer/clathrin_app_Ig-like.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR032570. SF1-HH.
IPR001878. Znf_CCHC.
[Graphical view]
PANTHERiPTHR11208:SF45. PTHR11208:SF45. 1 hit.
PfamiPF00013. KH_1. 1 hit.
PF16275. SF1-HH. 1 hit.
PF00098. zf-CCHC. 1 hit.
[Graphical view]
SMARTiSM00322. KH. 1 hit.
SM00343. ZnF_C2HC. 2 hits.
[Graphical view]
SUPFAMiSSF49348. SSF49348. 1 hit.
SSF54791. SSF54791. 1 hit.
SSF57756. SSF57756. 1 hit.
PROSITEiPS50158. ZF_CCHC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiBBP_YEAST
AccessioniPrimary (citable) accession number: Q12186
Secondary accession number(s): D6VYB4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 16, 2006
Last sequence update: November 1, 1996
Last modified: November 2, 2016
This is version 131 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 4380 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XII
    Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.