Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Branchpoint-bridging protein

Gene

MSL5

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Required for pre-spliceosome formation, which is the first step of pre-mRNA splicing. 2 commitment complexes, CC1 and CC2, have been defined. CC1 is a basal complex dependent only on the 5'-splice site. CC2 is a complex of lower mobility and is dependent on a branchpoint as well as a 5'-splice site region. This protein is involved in CC2 formation where it binds to the snRNP U1-associated protein PRP40, bridging the U1 snRNP-associated 5'-splice site and the MSL5-associated branch point 3' intron splice site. Involved in nuclear retention of pre-mRNA.3 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri271 – 28818CCHC-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri297 – 31418CCHC-type 2PROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  • pre-mRNA branch point binding Source: SGD
  • zinc ion binding Source: InterPro

GO - Biological processi

  • mRNA splicing, via spliceosome Source: SGD
Complete GO annotation...

Keywords - Biological processi

mRNA processing, mRNA splicing

Keywords - Ligandi

Metal-binding, RNA-binding, Zinc

Enzyme and pathway databases

BioCyciYEAST:G3O-32261-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Branchpoint-bridging protein
Alternative name(s):
Mud synthetic-lethal 5 protein
Splicing factor 1
Zinc finger protein BBP
Gene namesi
Name:MSL5
Synonyms:BBP, SF1
Ordered Locus Names:YLR116W
ORF Names:L2949
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XII

Organism-specific databases

EuPathDBiFungiDB:YLR116W.
SGDiS000004106. MSL5.

Subcellular locationi

GO - Cellular componenti

  • commitment complex Source: SGD
  • cytosol Source: SGD
  • nucleus Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi60 – 601R → G in MSL5-2; temperature-sensitive; growth defect; when associated with N-72 and S-155. 1 Publication
Mutagenesisi72 – 721I → N in MSL5-2; temperature-sensitive; growth defect; when associated with G-60 and S-155. 1 Publication
Mutagenesisi155 – 1551P → S in MSL5-2; temperature-sensitive; growth defect; when associated with G-60 and N-72. 1 Publication
Mutagenesisi195 – 1951V → D in MSL5-5; cold-sensitive; growth defect; when associated with V-258. 1 Publication
Mutagenesisi230 – 2301G → S in msl-5; loss of function. 1 Publication
Mutagenesisi258 – 2581E → V in MSL5-5; cold-sensitive; growth defect; when associated with D-195. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 476476Branchpoint-bridging proteinPRO_0000234564Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei93 – 931PhosphoserineCombined sources
Modified residuei95 – 951PhosphoserineCombined sources
Modified residuei100 – 1001PhosphotyrosineCombined sources
Modified residuei382 – 3821PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ12186.

PTM databases

iPTMnetiQ12186.

Interactioni

Subunit structurei

Interacts with MUD2 and PRP40. The proline-rich sequence of this protein interacts with the GYF domains of SMY2 and SYH1.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
MUD2P360845EBI-34012,EBI-11612

Protein-protein interaction databases

BioGridi31388. 72 interactions.
DIPiDIP-991N.
IntActiQ12186. 6 interactions.
MINTiMINT-489287.

Structurei

Secondary structure

1
476
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi148 – 1536Combined sources
Turni156 – 1583Combined sources
Helixi164 – 1696Combined sources
Helixi171 – 1733Combined sources
Helixi174 – 18310Combined sources
Beta strandi186 – 1927Combined sources
Helixi201 – 2033Combined sources
Turni206 – 2094Combined sources
Beta strandi212 – 22312Combined sources
Helixi224 – 24219Combined sources
Helixi250 – 26213Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3FMAX-ray2.50L/M/N/O/P440-450[»]
4WALX-ray2.20A144-271[»]
4WANX-ray1.80A/C/E/G144-271[»]
ProteinModelPortaliQ12186.
SMRiQ12186. Positions 50-316.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ12186.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini153 – 21967KHAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi363 – 474112Pro-richAdd
BLAST

Sequence similaritiesi

Belongs to the BBP/SF1 family.Curated
Contains 2 CCHC-type zinc fingers.PROSITE-ProRule annotation
Contains 1 KH domain.Curated

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri271 – 28818CCHC-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri297 – 31418CCHC-type 2PROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

GeneTreeiENSGT00550000074434.
HOGENOMiHOG000197271.
InParanoidiQ12186.
KOiK13095.
OMAiPPASHIS.
OrthoDBiEOG7WX0KW.

Family and domain databases

Gene3Di3.30.1370.10. 1 hit.
4.10.60.10. 1 hit.
InterProiIPR031150. BBP/SF1.
IPR013041. Coatomer/clathrin_app_Ig-like.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR032570. SF1-HH.
IPR001878. Znf_CCHC.
[Graphical view]
PANTHERiPTHR11208:SF45. PTHR11208:SF45. 1 hit.
PfamiPF00013. KH_1. 1 hit.
PF16275. SF1-HH. 1 hit.
PF00098. zf-CCHC. 1 hit.
[Graphical view]
SMARTiSM00322. KH. 1 hit.
SM00343. ZnF_C2HC. 2 hits.
[Graphical view]
SUPFAMiSSF49348. SSF49348. 1 hit.
SSF54791. SSF54791. 1 hit.
SSF57756. SSF57756. 1 hit.
PROSITEiPS50158. ZF_CCHC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q12186-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSFRRINSRY FENRKGSSME EKKAKVPPNV NLSLWRKNTV ESDVHRFNSL
60 70 80 90 100
PSKISGALTR EQIYSYQVMF RIQEITIKLR TNDFVPPSRK NRSPSPPPVY
110 120 130 140 150
DAQGKRTNTR EQRYRKKLED ERIKLVEIAL KTIPYFVPPD DYKRPTKFQD
160 170 180 190 200
KYYIPVDQYP DVNFVGLLLG PRGRTLRKLQ EDSNCKIAIR GRGSVKEGKN
210 220 230 240 250
ASDLPPGAMN FEDPLHCLII ADSEDKIQKG IKVCQNIVIK AVTSPEGQND
260 270 280 290 300
LKRGQLRELA ELNGTLREDN RPCPICGLKD HKRYDCPNRK IPNIQGIVCK
310 320 330 340 350
ICGQTGHFSR DCNSSSQRMS RFDRNATVNN SAPIQSNDVH YNSNTHPIQA
360 370 380 390 400
PKRSRYDNNS TEPPLKFPAS SRYAPSPSPP ASHISRQAQN VTPTPPPGLT
410 420 430 440 450
SSSFSSGVPG IAPPPLQSPP ESEQPKFSLP PPPGMTTVQS SIAPPPGLSG
460 470
PPGFSNNMGN DINKPTPPGL QGPPGL
Length:476
Mass (Da):53,034
Last modified:November 1, 1996 - v1
Checksum:iF94FF7175345C47D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X89514 Genomic DNA. Translation: CAA61695.1.
U53877 Genomic DNA. Translation: AAB82363.1.
Z73288 Genomic DNA. Translation: CAA97683.1.
BK006945 Genomic DNA. Translation: DAA09430.1.
PIRiS64953.
RefSeqiNP_013217.1. NM_001182003.1.

Genome annotation databases

EnsemblFungiiYLR116W; YLR116W; YLR116W.
GeneIDi850807.
KEGGisce:YLR116W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X89514 Genomic DNA. Translation: CAA61695.1.
U53877 Genomic DNA. Translation: AAB82363.1.
Z73288 Genomic DNA. Translation: CAA97683.1.
BK006945 Genomic DNA. Translation: DAA09430.1.
PIRiS64953.
RefSeqiNP_013217.1. NM_001182003.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3FMAX-ray2.50L/M/N/O/P440-450[»]
4WALX-ray2.20A144-271[»]
4WANX-ray1.80A/C/E/G144-271[»]
ProteinModelPortaliQ12186.
SMRiQ12186. Positions 50-316.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi31388. 72 interactions.
DIPiDIP-991N.
IntActiQ12186. 6 interactions.
MINTiMINT-489287.

PTM databases

iPTMnetiQ12186.

Proteomic databases

MaxQBiQ12186.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYLR116W; YLR116W; YLR116W.
GeneIDi850807.
KEGGisce:YLR116W.

Organism-specific databases

EuPathDBiFungiDB:YLR116W.
SGDiS000004106. MSL5.

Phylogenomic databases

GeneTreeiENSGT00550000074434.
HOGENOMiHOG000197271.
InParanoidiQ12186.
KOiK13095.
OMAiPPASHIS.
OrthoDBiEOG7WX0KW.

Enzyme and pathway databases

BioCyciYEAST:G3O-32261-MONOMER.

Miscellaneous databases

EvolutionaryTraceiQ12186.
NextBioi967037.
PROiQ12186.

Family and domain databases

Gene3Di3.30.1370.10. 1 hit.
4.10.60.10. 1 hit.
InterProiIPR031150. BBP/SF1.
IPR013041. Coatomer/clathrin_app_Ig-like.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR032570. SF1-HH.
IPR001878. Znf_CCHC.
[Graphical view]
PANTHERiPTHR11208:SF45. PTHR11208:SF45. 1 hit.
PfamiPF00013. KH_1. 1 hit.
PF16275. SF1-HH. 1 hit.
PF00098. zf-CCHC. 1 hit.
[Graphical view]
SMARTiSM00322. KH. 1 hit.
SM00343. ZnF_C2HC. 2 hits.
[Graphical view]
SUPFAMiSSF49348. SSF49348. 1 hit.
SSF54791. SSF54791. 1 hit.
SSF57756. SSF57756. 1 hit.
PROSITEiPS50158. ZF_CCHC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence analysis of a 37.6 kbp cosmid clone from the right arm of Saccharomyces cerevisiae chromosome XII, carrying YAP3, HOG1, SNR6, tRNA-Arg3 and 23 new open reading frames, among which several homologies to proteins involved in cell division control and to mammalian growth factors and other animal proteins are found."
    Verhasselt P., Volckaert G.
    Yeast 13:241-250(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 90840 / EAY235 / FY23.
  2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XII."
    Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H.
    , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D.
    Nature 387:87-90(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "Cross-intron bridging interactions in the yeast commitment complex are conserved in mammals."
    Abovich N., Rosbash M.
    Cell 89:403-412(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH MUD2 AND PRP40, MUTAGENESIS OF GLY-230.
  5. "Transient interaction of BBP/ScSF1 and Mud2 with the splicing machinery affects the kinetics of spliceosome assembly."
    Rutz B., Seraphin B.
    RNA 5:819-831(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH MUD2.
  6. "A dual role for BBP/ScSF1 in nuclear pre-mRNA retention and splicing."
    Rutz B., Seraphin B.
    EMBO J. 19:1873-1886(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, MUTAGENESIS OF ARG-60; ILE-72; PRO-155; VAL-195 AND GLU-258.
  7. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  8. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  9. "GYF domain proteomics reveals interaction sites in known and novel target proteins."
    Kofler M., Motzny K., Freund C.
    Mol. Cell. Proteomics 4:1797-1811(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH SMY2 AND SYH1.
  10. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-95, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  11. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-93; SER-95; TYR-100 AND SER-382, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiBBP_YEAST
AccessioniPrimary (citable) accession number: Q12186
Secondary accession number(s): D6VYB4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 16, 2006
Last sequence update: November 1, 1996
Last modified: May 11, 2016
This is version 127 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 4380 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XII
    Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.