ID FCY1_YEAST Reviewed; 158 AA. AC Q12178; DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot. DT 01-NOV-1996, sequence version 1. DT 16-JUN-2009, entry version 87. DE RecName: Full=Cytosine deaminase; DE EC=3.5.4.1; DE AltName: Full=Cytosine aminohydrolase; GN Name=FCY1; OrderedLocusNames=YPR062W; ORFNames=YP9499.17; OS Saccharomyces cerevisiae (Baker's yeast). OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; OC Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces. OX NCBI_TaxID=4932; RN [1] RP NUCLEOTIDE SEQUENCE [GENOMIC DNA]. RC STRAIN=ATCC 28383 / FL100 / VTT C-80102; RX MEDLINE=97153461; PubMed=9000374; DOI=10.1007/s002940050169; RA Erbs P., Exinger F., Jund R.; RT "Characterization of the Saccharomyces cerevisiae FCY1 gene encoding RT cytosine deaminase and its homologue FCA1 of Candida albicans."; RL Curr. Genet. 31:1-6(1997). RN [2] RP NUCLEOTIDE SEQUENCE [GENOMIC DNA]. RC STRAIN=334; RA Senter P.D., Su P.C.D., Marquardt H., Hayden M.S., Linsley P.S.; RT "Isolation of thermally stable cytosine deaminase from baker's RT yeast."; RL Submitted (AUG-1997) to the EMBL/GenBank/DDBJ databases. RN [3] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=ATCC 204511 / S288c / AB972; RX MEDLINE=97313271; PubMed=9169875; RA Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., RA Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., RA Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., RA Chung E., Churcher C.M., Coster F., Davis K., Davis R.W., RA Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., RA Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., RA Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., RA Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., RA Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., RA Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., RA Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., RA Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., RA Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., RA Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., RA Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., RA Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., RA Zollner A., Vo D.H., Hani J.; RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI."; RL Nature 387:103-105(1997). RN [4] RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS]. RX MEDLINE=22923965; PubMed=14562106; DOI=10.1038/nature02046; RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., RA Dephoure N., O'Shea E.K., Weissman J.S.; RT "Global analysis of protein expression in yeast."; RL Nature 425:737-741(2003). RN [5] RP X-RAY CRYSTALLOGRAPHY (1.6 ANGSTROMS). RX PubMed=12637534; DOI=10.1074/jbc.M300874200; RA Ko T.-P., Lin J.-J., Hu C.-Y., Hsu Y.-H., Wang A.H.-J., Liaw S.-H.; RT "Crystal structure of yeast cytosine deaminase. Insights into enzyme RT mechanism and evolution."; RL J. Biol. Chem. 278:19111-19117(2003). RN [6] RP X-RAY CRYSTALLOGRAPHY (1.14 ANGSTROMS) IN COMPLEX WITH SUBSTRATE RP ANALOG AND ZINC IONS, AND SUBUNIT. RX PubMed=12906827; DOI=10.1016/S0969-2126(03)00153-9; RA Ireton G.C., Black M.E., Stoddard B.L.; RT "The 1.14 A crystal structure of yeast cytosine deaminase: evolution RT of nucleotide salvage enzymes and implications for genetic RT chemotherapy."; RL Structure 11:961-972(2003). CC -!- FUNCTION: Converts cytosine to uracil or 5-methylcytosine to CC thymine by deaminating carbon number 4. CC -!- CATALYTIC ACTIVITY: Cytosine + H(2)O = uracil + NH(3). CC -!- COFACTOR: Zinc (By similarity). CC -!- PATHWAY: Pyrimidine metabolism; UMP biosynthesis via salvage CC pathway; uracil from cytosine: step 1/1. CC -!- SUBUNIT: Homodimer. CC -!- INTERACTION: CC Self; NbExp=1; IntAct=EBI-6851, EBI-6851; CC P53751:-; NbExp=1; IntAct=EBI-6851, EBI-28557; CC P09938:RNR2; NbExp=1; IntAct=EBI-6851, EBI-15240; CC -!- MISCELLANEOUS: Present with 5180 molecules/cell in log phase SD CC medium. CC -!- SIMILARITY: Belongs to the cytidine and deoxycytidylate deaminase CC family. CC ----------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution-NoDerivs License CC ----------------------------------------------------------------------- DR EMBL; U55193; AAC13409.1; -; Genomic_DNA. DR EMBL; AF005261; AAB67713.1; -; Genomic_DNA. DR EMBL; Z71255; CAA95006.1; -; Genomic_DNA. DR EMBL; Z49219; CAA89179.1; -; Genomic_DNA. DR PIR; S54083; S54083. DR RefSeq; NP_015387.1; -. DR PDB; 1OX7; X-ray; 1.43 A; A/B=1-158. DR PDB; 1P6O; X-ray; 1.14 A; A/B=1-158. DR PDB; 1RB7; X-ray; 2.10 A; A/B=1-158. DR PDB; 1UAQ; X-ray; 1.60 A; A/B=1-158. DR PDB; 1YSB; X-ray; 1.70 A; A/B=1-158. DR PDB; 1YSD; X-ray; 1.90 A; A/B=1-158. DR PDB; 2O3K; X-ray; 2.30 A; A/B=1-158. DR PDBsum; 1OX7; -. DR PDBsum; 1P6O; -. DR PDBsum; 1RB7; -. DR PDBsum; 1UAQ; -. DR PDBsum; 1YSB; -. DR PDBsum; 1YSD; -. DR PDBsum; 2O3K; -. DR DIP; DIP:1662N; -. DR IntAct; Q12178; 4. DR PeptideAtlas; Q12178; -. DR Ensembl; YPR062W; Saccharomyces cerevisiae. DR GeneID; 856175; -. DR GenomeReviews; U00094_GR; YPR062W. DR KEGG; sce:YPR062W; -. DR NMPDR; fig|4932.3.peg.6523; -. DR CYGD; YPR062w; -. DR SGD; S000006266; FCY1. DR HOGENOM; Q12178; -. DR OMA; Q12178; EMDCLEN. DR BRENDA; 3.5.4.1; 250. DR NextBio; 981337; -. DR ArrayExpress; Q12178; -. DR GermOnline; YPR062W; Saccharomyces cerevisiae. DR GO; GO:0005737; C:cytoplasm; IDA:SGD. DR GO; GO:0005634; C:nucleus; IDA:SGD. DR GO; GO:0004131; F:cytosine deaminase activity; IDA:SGD. DR GO; GO:0042802; F:identical protein binding; IPI:IntAct. DR GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-KW. DR GO; GO:0046087; P:cytidine metabolic process; IMP:SGD. DR GO; GO:0019858; P:cytosine metabolic process; IMP:SGD. DR GO; GO:0008655; P:pyrimidine salvage; IMP:SGD. DR InterPro; IPR016192; APOBEC/CMP_deaminase_Zn-bd. DR InterPro; IPR002125; CMP_dCMP_Zn_bd. DR Pfam; PF00383; dCMP_cyt_deam_1; 1. DR PROSITE; PS00903; CYT_DCMP_DEAMINASES; 1. PE 1: Evidence at protein level; KW 3D-structure; Complete proteome; Hydrolase; Metal-binding; Zinc. FT CHAIN 1 158 Cytosine deaminase. FT /FTId=PRO_0000171702. FT COMPBIAS 129 132 Poly-Val. FT ACT_SITE 64 64 Proton donor. FT METAL 62 62 Zinc; catalytic. FT METAL 91 91 Zinc; catalytic. FT METAL 94 94 Zinc; catalytic. FT BINDING 51 51 Substrate. FT BINDING 155 155 Substrate. FT HELIX 11 27 FT STRAND 34 39 FT TURN 40 42 FT STRAND 45 50 FT HELIX 53 56 FT HELIX 63 71 FT HELIX 76 79 FT STRAND 82 88 FT HELIX 92 101 FT STRAND 105 113 FT HELIX 118 124 FT STRAND 128 131 FT HELIX 135 147 FT HELIX 149 155 SQ SEQUENCE 158 AA; 17507 MW; 19DB41E9AF26ADDC CRC64; MVTGGMASKW DQKGMDIAYE EAALGYKEGG VPIGGCLINN KDGSVLGRGH NMRFQKGSAT LHGEISTLEN CGRLEGKVYK DTTLYTTLSP CDMCTGAIIM YGIPRCVVGE NVNFKSKGEK YLQTRGHEVV VVDDERCKKI MKQFIDERPQ DWFEDIGE //