Q12178 (FCY1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cytosine deaminase
EC=3.5.4.1

Alternative name(s):
Cytosine aminohydrolase

Gene names

Name:	FCY1
Ordered Locus Names:	YPR062W
ORF Names:	YP9499.17

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	158 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Converts cytosine to uracil or 5-methylcytosine to thymine by deaminating carbon number 4.
Catalytic activity	Cytosine + H2O = uracil + NH3.
Cofactor	Zinc By similarity.
Pathway	Pyrimidine metabolism; UMP biosynthesis via salvage pathway; uracil from cytosine: step 1/1.
Subunit structure	Homodimer. Ref.7
Miscellaneous	Present with 5180 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the cytidine and deoxycytidylate deaminase family.

Ontologies

Keywords
Ligand	Metal-binding Zinc
Molecular function	Hydrolase
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	UMP salvage Inferred from electronic annotation. Source: UniProtKB-UniPathway cytidine metabolic process Inferred from mutant phenotype PubMed 10501935. Source: SGD cytosine metabolic process Inferred from mutant phenotype Ref.1. Source: SGD pyrimidine-containing compound salvage Inferred from mutant phenotype Ref.1. Source: SGD
Cellular_component	cytoplasm Inferred from direct assay PubMed 14562095. Source: SGD nucleus Inferred from direct assay PubMed 14562095. Source: SGD
Molecular_function	cytosine deaminase activity Inferred from direct assay PubMed 15535715Ref.1. Source: SGD zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 158

158

Cytosine deaminase

PRO_0000171702

Regions

Compositional bias

129 – 132

4

Poly-Val

Sites

Active site

64

1

Proton donor

Metal binding

62

1

Zinc; catalytic

Metal binding

91

1

Zinc; catalytic

Metal binding

94

1

Zinc; catalytic

Binding site

51

1

Substrate

Binding site

155

1

Substrate

Secondary structure

1

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158

Helix Strand Turn

Details...

Helix

11 – 27

17

Beta strand

34 – 39

6

Turn

40 – 42

3

Beta strand

45 – 50

6

Helix

53 – 56

4

Helix

63 – 71

9

Helix

76 – 79

4

Beta strand

82 – 88

7

Helix

92 – 101

10

Beta strand

105 – 113

9

Helix

118 – 124

7

Beta strand

128 – 131

4

Helix

135 – 147

13

Helix

149 – 155

7

Sequences

	Sequence		Length	Mass (Da)
	Q12178 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 19DB41E9AF26ADDC	FASTA	158	17,507
	10 20 30 40 50 60 MVTGGMASKW DQKGMDIAYE EAALGYKEGG VPIGGCLINN KDGSVLGRGH NMRFQKGSAT 70 80 90 100 110 120 LHGEISTLEN CGRLEGKVYK DTTLYTTLSP CDMCTGAIIM YGIPRCVVGE NVNFKSKGEK 130 140 150 YLQTRGHEVV VVDDERCKKI MKQFIDERPQ DWFEDIGE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization of the Saccharomyces cerevisiae FCY1 gene encoding cytosine deaminase and its homologue FCA1 of Candida albicans." Erbs P., Exinger F., Jund R. Curr. Genet. 31:1-6(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 28383 / FL100 / VTT C-80102.
[2]	"Isolation of thermally stable cytosine deaminase from baker's yeast." Senter P.D., Su P.C.D., Marquardt H., Hayden M.S., Linsley P.S. Submitted (AUG-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 334.
[3]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI." Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M. , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J. Nature 387:103-105(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[4]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]	"Crystal structure of yeast cytosine deaminase. Insights into enzyme mechanism and evolution." Ko T.-P., Lin J.-J., Hu C.-Y., Hsu Y.-H., Wang A.H.-J., Liaw S.-H. J. Biol. Chem. 278:19111-19117(2003) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.6 ANGSTROMS).
[7]	"The 1.14 A crystal structure of yeast cytosine deaminase: evolution of nucleotide salvage enzymes and implications for genetic chemotherapy." Ireton G.C., Black M.E., Stoddard B.L. Structure 11:961-972(2003) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.14 ANGSTROMS) IN COMPLEX WITH SUBSTRATE ANALOG AND ZINC IONS, SUBUNIT.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

U55193 Genomic DNA. Translation: AAC13409.1.
AF005261 Genomic DNA. Translation: AAB67713.1.
Z71255 Genomic DNA. Translation: CAA95006.1.
Z49219 Genomic DNA. Translation: CAA89179.1.
BK006949 Genomic DNA. Translation: DAA11483.1.

PIR

S54083.

RefSeq

NP_015387.1. NM_001184159.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1OX7	X-ray	1.43	A/B	1-158	[»]
1P6O	X-ray	1.14	A/B	1-158	[»]
1RB7	X-ray	2.10	A/B	1-158	[»]
1UAQ	X-ray	1.60	A/B	1-158	[»]
1YSB	X-ray	1.70	A/B	1-158	[»]
1YSD	X-ray	1.90	A/B	1-158	[»]
2O3K	X-ray	2.30	A/B	1-158	[»]

ProteinModelPortal

Q12178.

SMR

Q12178. Positions 1-158.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-1662N.

IntAct

Q12178. 2 interactions.

MINT

MINT-392713.

STRING

4932.YPR062W.

Proteomic databases

PaxDb

Q12178.

PeptideAtlas

Q12178.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblFungi

YPR062W; YPR062W; YPR062W.

GeneID

856175.

KEGG

sce:YPR062W.

Organism-specific databases

CYGD

YPR062w.

SGD

S000006266. FCY1.

Phylogenomic databases

eggNOG

COG0590.

GeneTree

ENSGT00390000014126.

HOGENOM

HOG000085050.

KO

K01485.

OMA

AILHAEM.

OrthoDB

EOG40ZV73.

Enzyme and pathway databases

UniPathway

UPA00574; UER00635.

Gene expression databases

Genevestigator

Q12178.

GermOnline

YPR062W. Saccharomyces cerevisiae.

Family and domain databases

InterPro

IPR016192. APOBEC/CMP_deaminase_Zn-bd.
IPR002125. CMP_dCMP_Zn-bd.
IPR016193. Cytidine_deaminase-like.
[Graphical view]

Pfam

PF00383. dCMP_cyt_deam_1. 1 hit.
[Graphical view]

SUPFAM

SSF53927. Cytidine_deaminase-like. 1 hit.

PROSITE

PS00903. CYT_DCMP_DEAMINASES. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

Q12178.

NextBio

981337.

Entry information

Entry name

FCY1_YEAST

Accession

Primary (citable) accession number: Q12178
Secondary accession number(s): D6W467

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1996
Last modified:	April 3, 2013
This is version 122 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program