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Protein

Cytosine deaminase

Gene

FCY1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Converts cytosine to uracil or 5-methylcytosine to thymine by deaminating carbon number 4.

Catalytic activityi

Cytosine + H2O = uracil + NH3.

Cofactori

Zn2+By similarity

Pathwayi: UMP biosynthesis via salvage pathway

This protein is involved in step 1 of the subpathway that synthesizes uracil from cytosine.
Proteins known to be involved in this subpathway in this organism are:
  1. Cytosine deaminase (FCY1)
This subpathway is part of the pathway UMP biosynthesis via salvage pathway, which is itself part of Pyrimidine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes uracil from cytosine, the pathway UMP biosynthesis via salvage pathway and in Pyrimidine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei51Substrate1
Metal bindingi62Zinc; catalytic1
Active sitei64Proton donor1
Metal bindingi91Zinc; catalytic1
Metal bindingi94Zinc; catalytic1
Binding sitei155Substrate1

GO - Molecular functioni

  • cytosine deaminase activity Source: SGD
  • zinc ion binding Source: InterPro

GO - Biological processi

  • cytidine metabolic process Source: SGD
  • cytosine metabolic process Source: SGD
  • pyrimidine-containing compound salvage Source: SGD
  • RNA modification Source: GO_Central
  • UMP salvage Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciMetaCyc:YPR062W-MONOMER.
YEAST:YPR062W-MONOMER.
BRENDAi3.5.4.1. 984.
UniPathwayiUPA00574; UER00635.

Names & Taxonomyi

Protein namesi
Recommended name:
Cytosine deaminase (EC:3.5.4.1)
Alternative name(s):
Cytosine aminohydrolase
Gene namesi
Name:FCY1
Ordered Locus Names:YPR062W
ORF Names:YP9499.17
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XVI

Organism-specific databases

EuPathDBiFungiDB:YPR062W.
SGDiS000006266. FCY1.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001717021 – 158Cytosine deaminaseAdd BLAST158

Proteomic databases

MaxQBiQ12178.
PRIDEiQ12178.

Interactioni

Subunit structurei

Homodimer.1 Publication

Protein-protein interaction databases

BioGridi36235. 24 interactors.
DIPiDIP-1662N.
IntActiQ12178. 2 interactors.
MINTiMINT-392713.

Structurei

Secondary structure

1158
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi11 – 27Combined sources17
Beta strandi34 – 39Combined sources6
Turni40 – 42Combined sources3
Beta strandi45 – 50Combined sources6
Helixi53 – 56Combined sources4
Helixi63 – 71Combined sources9
Helixi76 – 79Combined sources4
Beta strandi82 – 88Combined sources7
Helixi92 – 101Combined sources10
Beta strandi105 – 113Combined sources9
Helixi118 – 124Combined sources7
Beta strandi128 – 131Combined sources4
Helixi135 – 147Combined sources13
Helixi149 – 155Combined sources7

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1OX7X-ray1.43A/B1-158[»]
1P6OX-ray1.14A/B1-158[»]
1RB7X-ray2.10A/B1-158[»]
1UAQX-ray1.60A/B1-158[»]
1YSBX-ray1.70A/B1-158[»]
1YSDX-ray1.90A/B1-158[»]
2O3KX-ray2.30A/B1-158[»]
ProteinModelPortaliQ12178.
SMRiQ12178.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ12178.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini9 – 129CMP/dCMP-type deaminasePROSITE-ProRule annotationAdd BLAST121

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi129 – 132Poly-Val4

Sequence similaritiesi

Contains 1 CMP/dCMP-type deaminase domain.PROSITE-ProRule annotation

Phylogenomic databases

HOGENOMiHOG000085050.
InParanoidiQ12178.
KOiK01485.
OMAiILLYKIP.
OrthoDBiEOG092C4YNP.

Family and domain databases

InterProiIPR016192. APOBEC/CMP_deaminase_Zn-bd.
IPR002125. CMP_dCMP_Zn-bd.
IPR016193. Cytidine_deaminase-like.
[Graphical view]
PfamiPF00383. dCMP_cyt_deam_1. 1 hit.
[Graphical view]
SUPFAMiSSF53927. SSF53927. 1 hit.
PROSITEiPS00903. CYT_DCMP_DEAMINASES_1. 1 hit.
PS51747. CYT_DCMP_DEAMINASES_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q12178-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVTGGMASKW DQKGMDIAYE EAALGYKEGG VPIGGCLINN KDGSVLGRGH
60 70 80 90 100
NMRFQKGSAT LHGEISTLEN CGRLEGKVYK DTTLYTTLSP CDMCTGAIIM
110 120 130 140 150
YGIPRCVVGE NVNFKSKGEK YLQTRGHEVV VVDDERCKKI MKQFIDERPQ

DWFEDIGE
Length:158
Mass (Da):17,507
Last modified:November 1, 1996 - v1
Checksum:i19DB41E9AF26ADDC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U55193 Genomic DNA. Translation: AAC13409.1.
AF005261 Genomic DNA. Translation: AAB67713.1.
Z71255 Genomic DNA. Translation: CAA95006.1.
Z49219 Genomic DNA. Translation: CAA89179.1.
BK006949 Genomic DNA. Translation: DAA11483.1.
PIRiS54083.
RefSeqiNP_015387.1. NM_001184159.1.

Genome annotation databases

EnsemblFungiiYPR062W; YPR062W; YPR062W.
GeneIDi856175.
KEGGisce:YPR062W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U55193 Genomic DNA. Translation: AAC13409.1.
AF005261 Genomic DNA. Translation: AAB67713.1.
Z71255 Genomic DNA. Translation: CAA95006.1.
Z49219 Genomic DNA. Translation: CAA89179.1.
BK006949 Genomic DNA. Translation: DAA11483.1.
PIRiS54083.
RefSeqiNP_015387.1. NM_001184159.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1OX7X-ray1.43A/B1-158[»]
1P6OX-ray1.14A/B1-158[»]
1RB7X-ray2.10A/B1-158[»]
1UAQX-ray1.60A/B1-158[»]
1YSBX-ray1.70A/B1-158[»]
1YSDX-ray1.90A/B1-158[»]
2O3KX-ray2.30A/B1-158[»]
ProteinModelPortaliQ12178.
SMRiQ12178.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36235. 24 interactors.
DIPiDIP-1662N.
IntActiQ12178. 2 interactors.
MINTiMINT-392713.

Proteomic databases

MaxQBiQ12178.
PRIDEiQ12178.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYPR062W; YPR062W; YPR062W.
GeneIDi856175.
KEGGisce:YPR062W.

Organism-specific databases

EuPathDBiFungiDB:YPR062W.
SGDiS000006266. FCY1.

Phylogenomic databases

HOGENOMiHOG000085050.
InParanoidiQ12178.
KOiK01485.
OMAiILLYKIP.
OrthoDBiEOG092C4YNP.

Enzyme and pathway databases

UniPathwayiUPA00574; UER00635.
BioCyciMetaCyc:YPR062W-MONOMER.
YEAST:YPR062W-MONOMER.
BRENDAi3.5.4.1. 984.

Miscellaneous databases

EvolutionaryTraceiQ12178.
PROiQ12178.

Family and domain databases

InterProiIPR016192. APOBEC/CMP_deaminase_Zn-bd.
IPR002125. CMP_dCMP_Zn-bd.
IPR016193. Cytidine_deaminase-like.
[Graphical view]
PfamiPF00383. dCMP_cyt_deam_1. 1 hit.
[Graphical view]
SUPFAMiSSF53927. SSF53927. 1 hit.
PROSITEiPS00903. CYT_DCMP_DEAMINASES_1. 1 hit.
PS51747. CYT_DCMP_DEAMINASES_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiFCY1_YEAST
AccessioniPrimary (citable) accession number: Q12178
Secondary accession number(s): D6W467
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: November 2, 2016
This is version 147 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 5180 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  5. Yeast chromosome XVI
    Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.