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Q12178

- FCY1_YEAST

UniProt

Q12178 - FCY1_YEAST

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Protein

Cytosine deaminase

Gene

FCY1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Converts cytosine to uracil or 5-methylcytosine to thymine by deaminating carbon number 4.

Catalytic activityi

Cytosine + H2O = uracil + NH3.

Cofactori

Zinc.By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei51 – 511Substrate
Metal bindingi62 – 621Zinc; catalytic
Active sitei64 – 641Proton donor
Metal bindingi91 – 911Zinc; catalytic
Metal bindingi94 – 941Zinc; catalytic
Binding sitei155 – 1551Substrate

GO - Molecular functioni

  1. cytosine deaminase activity Source: SGD
  2. zinc ion binding Source: InterPro

GO - Biological processi

  1. cytidine metabolic process Source: SGD
  2. cytosine metabolic process Source: SGD
  3. pyrimidine-containing compound salvage Source: SGD
  4. UMP salvage Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciMetaCyc:YPR062W-MONOMER.
YEAST:YPR062W-MONOMER.
UniPathwayiUPA00574; UER00635.

Names & Taxonomyi

Protein namesi
Recommended name:
Cytosine deaminase (EC:3.5.4.1)
Alternative name(s):
Cytosine aminohydrolase
Gene namesi
Name:FCY1
Ordered Locus Names:YPR062W
ORF Names:YP9499.17
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XVI

Organism-specific databases

CYGDiYPR062w.
SGDiS000006266. FCY1.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 158158Cytosine deaminasePRO_0000171702Add
BLAST

Proteomic databases

MaxQBiQ12178.
PaxDbiQ12178.
PeptideAtlasiQ12178.

Expressioni

Gene expression databases

GenevestigatoriQ12178.

Interactioni

Subunit structurei

Homodimer.1 Publication

Protein-protein interaction databases

BioGridi36235. 25 interactions.
DIPiDIP-1662N.
IntActiQ12178. 2 interactions.
MINTiMINT-392713.
STRINGi4932.YPR062W.

Structurei

Secondary structure

1
158
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi11 – 2717
Beta strandi34 – 396
Turni40 – 423
Beta strandi45 – 506
Helixi53 – 564
Helixi63 – 719
Helixi76 – 794
Beta strandi82 – 887
Helixi92 – 10110
Beta strandi105 – 1139
Helixi118 – 1247
Beta strandi128 – 1314
Helixi135 – 14713
Helixi149 – 1557

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1OX7X-ray1.43A/B1-158[»]
1P6OX-ray1.14A/B1-158[»]
1RB7X-ray2.10A/B1-158[»]
1UAQX-ray1.60A/B1-158[»]
1YSBX-ray1.70A/B1-158[»]
1YSDX-ray1.90A/B1-158[»]
2O3KX-ray2.30A/B1-158[»]
ProteinModelPortaliQ12178.
SMRiQ12178. Positions 1-158.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ12178.

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi129 – 1324Poly-Val

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0590.
HOGENOMiHOG000085050.
InParanoidiQ12178.
KOiK01485.
OMAiRININAN.
OrthoDBiEOG7R2BX1.

Family and domain databases

InterProiIPR016192. APOBEC/CMP_deaminase_Zn-bd.
IPR002125. CMP_dCMP_Zn-bd.
IPR016193. Cytidine_deaminase-like.
[Graphical view]
PfamiPF00383. dCMP_cyt_deam_1. 1 hit.
[Graphical view]
SUPFAMiSSF53927. SSF53927. 1 hit.
PROSITEiPS00903. CYT_DCMP_DEAMINASES. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q12178-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MVTGGMASKW DQKGMDIAYE EAALGYKEGG VPIGGCLINN KDGSVLGRGH
60 70 80 90 100
NMRFQKGSAT LHGEISTLEN CGRLEGKVYK DTTLYTTLSP CDMCTGAIIM
110 120 130 140 150
YGIPRCVVGE NVNFKSKGEK YLQTRGHEVV VVDDERCKKI MKQFIDERPQ

DWFEDIGE
Length:158
Mass (Da):17,507
Last modified:November 1, 1996 - v1
Checksum:i19DB41E9AF26ADDC
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U55193 Genomic DNA. Translation: AAC13409.1.
AF005261 Genomic DNA. Translation: AAB67713.1.
Z71255 Genomic DNA. Translation: CAA95006.1.
Z49219 Genomic DNA. Translation: CAA89179.1.
BK006949 Genomic DNA. Translation: DAA11483.1.
PIRiS54083.
RefSeqiNP_015387.1. NM_001184159.1.

Genome annotation databases

EnsemblFungiiYPR062W; YPR062W; YPR062W.
GeneIDi856175.
KEGGisce:YPR062W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U55193 Genomic DNA. Translation: AAC13409.1 .
AF005261 Genomic DNA. Translation: AAB67713.1 .
Z71255 Genomic DNA. Translation: CAA95006.1 .
Z49219 Genomic DNA. Translation: CAA89179.1 .
BK006949 Genomic DNA. Translation: DAA11483.1 .
PIRi S54083.
RefSeqi NP_015387.1. NM_001184159.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1OX7 X-ray 1.43 A/B 1-158 [» ]
1P6O X-ray 1.14 A/B 1-158 [» ]
1RB7 X-ray 2.10 A/B 1-158 [» ]
1UAQ X-ray 1.60 A/B 1-158 [» ]
1YSB X-ray 1.70 A/B 1-158 [» ]
1YSD X-ray 1.90 A/B 1-158 [» ]
2O3K X-ray 2.30 A/B 1-158 [» ]
ProteinModelPortali Q12178.
SMRi Q12178. Positions 1-158.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 36235. 25 interactions.
DIPi DIP-1662N.
IntActi Q12178. 2 interactions.
MINTi MINT-392713.
STRINGi 4932.YPR062W.

Proteomic databases

MaxQBi Q12178.
PaxDbi Q12178.
PeptideAtlasi Q12178.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YPR062W ; YPR062W ; YPR062W .
GeneIDi 856175.
KEGGi sce:YPR062W.

Organism-specific databases

CYGDi YPR062w.
SGDi S000006266. FCY1.

Phylogenomic databases

eggNOGi COG0590.
HOGENOMi HOG000085050.
InParanoidi Q12178.
KOi K01485.
OMAi RININAN.
OrthoDBi EOG7R2BX1.

Enzyme and pathway databases

UniPathwayi UPA00574 ; UER00635 .
BioCyci MetaCyc:YPR062W-MONOMER.
YEAST:YPR062W-MONOMER.

Miscellaneous databases

EvolutionaryTracei Q12178.
NextBioi 981337.

Gene expression databases

Genevestigatori Q12178.

Family and domain databases

InterProi IPR016192. APOBEC/CMP_deaminase_Zn-bd.
IPR002125. CMP_dCMP_Zn-bd.
IPR016193. Cytidine_deaminase-like.
[Graphical view ]
Pfami PF00383. dCMP_cyt_deam_1. 1 hit.
[Graphical view ]
SUPFAMi SSF53927. SSF53927. 1 hit.
PROSITEi PS00903. CYT_DCMP_DEAMINASES. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Characterization of the Saccharomyces cerevisiae FCY1 gene encoding cytosine deaminase and its homologue FCA1 of Candida albicans."
    Erbs P., Exinger F., Jund R.
    Curr. Genet. 31:1-6(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 28383 / FL100 / VTT C-80102.
  2. "Isolation of thermally stable cytosine deaminase from baker's yeast."
    Senter P.D., Su P.C.D., Marquardt H., Hayden M.S., Linsley P.S.
    Submitted (AUG-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 334.
  3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI."
    Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M.
    , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J.
    Nature 387:103-105(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  6. "Crystal structure of yeast cytosine deaminase. Insights into enzyme mechanism and evolution."
    Ko T.-P., Lin J.-J., Hu C.-Y., Hsu Y.-H., Wang A.H.-J., Liaw S.-H.
    J. Biol. Chem. 278:19111-19117(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.6 ANGSTROMS).
  7. "The 1.14 A crystal structure of yeast cytosine deaminase: evolution of nucleotide salvage enzymes and implications for genetic chemotherapy."
    Ireton G.C., Black M.E., Stoddard B.L.
    Structure 11:961-972(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.14 ANGSTROMS) IN COMPLEX WITH SUBSTRATE ANALOG AND ZINC IONS, SUBUNIT.

Entry informationi

Entry nameiFCY1_YEAST
AccessioniPrimary (citable) accession number: Q12178
Secondary accession number(s): D6W467
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: October 29, 2014
This is version 131 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 5180 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  5. Yeast chromosome XVI
    Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

External Data

Dasty 3