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Q12178

- FCY1_YEAST

UniProt

Q12178 - FCY1_YEAST

Protein

Cytosine deaminase

Gene

FCY1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 130 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Converts cytosine to uracil or 5-methylcytosine to thymine by deaminating carbon number 4.

    Catalytic activityi

    Cytosine + H2O = uracil + NH3.

    Cofactori

    Zinc.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei51 – 511Substrate
    Metal bindingi62 – 621Zinc; catalytic
    Active sitei64 – 641Proton donor
    Metal bindingi91 – 911Zinc; catalytic
    Metal bindingi94 – 941Zinc; catalytic
    Binding sitei155 – 1551Substrate

    GO - Molecular functioni

    1. cytosine deaminase activity Source: SGD
    2. zinc ion binding Source: InterPro

    GO - Biological processi

    1. cytidine metabolic process Source: SGD
    2. cytosine metabolic process Source: SGD
    3. pyrimidine-containing compound salvage Source: SGD
    4. UMP salvage Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Ligandi

    Metal-binding, Zinc

    Enzyme and pathway databases

    BioCyciMetaCyc:YPR062W-MONOMER.
    YEAST:YPR062W-MONOMER.
    UniPathwayiUPA00574; UER00635.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cytosine deaminase (EC:3.5.4.1)
    Alternative name(s):
    Cytosine aminohydrolase
    Gene namesi
    Name:FCY1
    Ordered Locus Names:YPR062W
    ORF Names:YP9499.17
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XVI

    Organism-specific databases

    CYGDiYPR062w.
    SGDiS000006266. FCY1.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 158158Cytosine deaminasePRO_0000171702Add
    BLAST

    Proteomic databases

    MaxQBiQ12178.
    PaxDbiQ12178.
    PeptideAtlasiQ12178.

    Expressioni

    Gene expression databases

    GenevestigatoriQ12178.

    Interactioni

    Subunit structurei

    Homodimer.1 Publication

    Protein-protein interaction databases

    BioGridi36235. 25 interactions.
    DIPiDIP-1662N.
    IntActiQ12178. 2 interactions.
    MINTiMINT-392713.
    STRINGi4932.YPR062W.

    Structurei

    Secondary structure

    1
    158
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi11 – 2717
    Beta strandi34 – 396
    Turni40 – 423
    Beta strandi45 – 506
    Helixi53 – 564
    Helixi63 – 719
    Helixi76 – 794
    Beta strandi82 – 887
    Helixi92 – 10110
    Beta strandi105 – 1139
    Helixi118 – 1247
    Beta strandi128 – 1314
    Helixi135 – 14713
    Helixi149 – 1557

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1OX7X-ray1.43A/B1-158[»]
    1P6OX-ray1.14A/B1-158[»]
    1RB7X-ray2.10A/B1-158[»]
    1UAQX-ray1.60A/B1-158[»]
    1YSBX-ray1.70A/B1-158[»]
    1YSDX-ray1.90A/B1-158[»]
    2O3KX-ray2.30A/B1-158[»]
    ProteinModelPortaliQ12178.
    SMRiQ12178. Positions 1-158.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ12178.

    Family & Domainsi

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi129 – 1324Poly-Val

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0590.
    HOGENOMiHOG000085050.
    KOiK01485.
    OMAiRININAN.
    OrthoDBiEOG7R2BX1.

    Family and domain databases

    InterProiIPR016192. APOBEC/CMP_deaminase_Zn-bd.
    IPR002125. CMP_dCMP_Zn-bd.
    IPR016193. Cytidine_deaminase-like.
    [Graphical view]
    PfamiPF00383. dCMP_cyt_deam_1. 1 hit.
    [Graphical view]
    SUPFAMiSSF53927. SSF53927. 1 hit.
    PROSITEiPS00903. CYT_DCMP_DEAMINASES. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q12178-1 [UniParc]FASTAAdd to Basket

    « Hide

    MVTGGMASKW DQKGMDIAYE EAALGYKEGG VPIGGCLINN KDGSVLGRGH    50
    NMRFQKGSAT LHGEISTLEN CGRLEGKVYK DTTLYTTLSP CDMCTGAIIM 100
    YGIPRCVVGE NVNFKSKGEK YLQTRGHEVV VVDDERCKKI MKQFIDERPQ 150
    DWFEDIGE 158
    Length:158
    Mass (Da):17,507
    Last modified:November 1, 1996 - v1
    Checksum:i19DB41E9AF26ADDC
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U55193 Genomic DNA. Translation: AAC13409.1.
    AF005261 Genomic DNA. Translation: AAB67713.1.
    Z71255 Genomic DNA. Translation: CAA95006.1.
    Z49219 Genomic DNA. Translation: CAA89179.1.
    BK006949 Genomic DNA. Translation: DAA11483.1.
    PIRiS54083.
    RefSeqiNP_015387.1. NM_001184159.1.

    Genome annotation databases

    EnsemblFungiiYPR062W; YPR062W; YPR062W.
    GeneIDi856175.
    KEGGisce:YPR062W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U55193 Genomic DNA. Translation: AAC13409.1 .
    AF005261 Genomic DNA. Translation: AAB67713.1 .
    Z71255 Genomic DNA. Translation: CAA95006.1 .
    Z49219 Genomic DNA. Translation: CAA89179.1 .
    BK006949 Genomic DNA. Translation: DAA11483.1 .
    PIRi S54083.
    RefSeqi NP_015387.1. NM_001184159.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1OX7 X-ray 1.43 A/B 1-158 [» ]
    1P6O X-ray 1.14 A/B 1-158 [» ]
    1RB7 X-ray 2.10 A/B 1-158 [» ]
    1UAQ X-ray 1.60 A/B 1-158 [» ]
    1YSB X-ray 1.70 A/B 1-158 [» ]
    1YSD X-ray 1.90 A/B 1-158 [» ]
    2O3K X-ray 2.30 A/B 1-158 [» ]
    ProteinModelPortali Q12178.
    SMRi Q12178. Positions 1-158.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 36235. 25 interactions.
    DIPi DIP-1662N.
    IntActi Q12178. 2 interactions.
    MINTi MINT-392713.
    STRINGi 4932.YPR062W.

    Proteomic databases

    MaxQBi Q12178.
    PaxDbi Q12178.
    PeptideAtlasi Q12178.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YPR062W ; YPR062W ; YPR062W .
    GeneIDi 856175.
    KEGGi sce:YPR062W.

    Organism-specific databases

    CYGDi YPR062w.
    SGDi S000006266. FCY1.

    Phylogenomic databases

    eggNOGi COG0590.
    HOGENOMi HOG000085050.
    KOi K01485.
    OMAi RININAN.
    OrthoDBi EOG7R2BX1.

    Enzyme and pathway databases

    UniPathwayi UPA00574 ; UER00635 .
    BioCyci MetaCyc:YPR062W-MONOMER.
    YEAST:YPR062W-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei Q12178.
    NextBioi 981337.

    Gene expression databases

    Genevestigatori Q12178.

    Family and domain databases

    InterProi IPR016192. APOBEC/CMP_deaminase_Zn-bd.
    IPR002125. CMP_dCMP_Zn-bd.
    IPR016193. Cytidine_deaminase-like.
    [Graphical view ]
    Pfami PF00383. dCMP_cyt_deam_1. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53927. SSF53927. 1 hit.
    PROSITEi PS00903. CYT_DCMP_DEAMINASES. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Characterization of the Saccharomyces cerevisiae FCY1 gene encoding cytosine deaminase and its homologue FCA1 of Candida albicans."
      Erbs P., Exinger F., Jund R.
      Curr. Genet. 31:1-6(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 28383 / FL100 / VTT C-80102.
    2. "Isolation of thermally stable cytosine deaminase from baker's yeast."
      Senter P.D., Su P.C.D., Marquardt H., Hayden M.S., Linsley P.S.
      Submitted (AUG-1997) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: 334.
    3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI."
      Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M.
      , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J.
      Nature 387:103-105(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    6. "Crystal structure of yeast cytosine deaminase. Insights into enzyme mechanism and evolution."
      Ko T.-P., Lin J.-J., Hu C.-Y., Hsu Y.-H., Wang A.H.-J., Liaw S.-H.
      J. Biol. Chem. 278:19111-19117(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.6 ANGSTROMS).
    7. "The 1.14 A crystal structure of yeast cytosine deaminase: evolution of nucleotide salvage enzymes and implications for genetic chemotherapy."
      Ireton G.C., Black M.E., Stoddard B.L.
      Structure 11:961-972(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.14 ANGSTROMS) IN COMPLEX WITH SUBSTRATE ANALOG AND ZINC IONS, SUBUNIT.

    Entry informationi

    Entry nameiFCY1_YEAST
    AccessioniPrimary (citable) accession number: Q12178
    Secondary accession number(s): D6W467
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 130 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 5180 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families
    4. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    5. Yeast chromosome XVI
      Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

    External Data

    Dasty 3