Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Cytosine deaminase

Gene

FCY1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Converts cytosine to uracil or 5-methylcytosine to thymine by deaminating carbon number 4.

Catalytic activityi

Cytosine + H2O = uracil + NH3.

Cofactori

Zn2+By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei51 – 511Substrate
Metal bindingi62 – 621Zinc; catalytic
Active sitei64 – 641Proton donor
Metal bindingi91 – 911Zinc; catalytic
Metal bindingi94 – 941Zinc; catalytic
Binding sitei155 – 1551Substrate

GO - Molecular functioni

  1. cytosine deaminase activity Source: SGD
  2. zinc ion binding Source: InterPro

GO - Biological processi

  1. cytidine metabolic process Source: SGD
  2. cytosine metabolic process Source: SGD
  3. pyrimidine-containing compound salvage Source: SGD
  4. UMP salvage Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciMetaCyc:YPR062W-MONOMER.
YEAST:YPR062W-MONOMER.
BRENDAi3.5.4.1. 984.
UniPathwayiUPA00574; UER00635.

Names & Taxonomyi

Protein namesi
Recommended name:
Cytosine deaminase (EC:3.5.4.1)
Alternative name(s):
Cytosine aminohydrolase
Gene namesi
Name:FCY1
Ordered Locus Names:YPR062W
ORF Names:YP9499.17
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311 Componenti: Chromosome XVI

Organism-specific databases

CYGDiYPR062w.
SGDiS000006266. FCY1.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 158158Cytosine deaminasePRO_0000171702Add
BLAST

Proteomic databases

MaxQBiQ12178.
PaxDbiQ12178.
PeptideAtlasiQ12178.

Expressioni

Gene expression databases

GenevestigatoriQ12178.

Interactioni

Subunit structurei

Homodimer.1 Publication

Protein-protein interaction databases

BioGridi36235. 25 interactions.
DIPiDIP-1662N.
IntActiQ12178. 2 interactions.
MINTiMINT-392713.
STRINGi4932.YPR062W.

Structurei

Secondary structure

1
158
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi11 – 2717Combined sources
Beta strandi34 – 396Combined sources
Turni40 – 423Combined sources
Beta strandi45 – 506Combined sources
Helixi53 – 564Combined sources
Helixi63 – 719Combined sources
Helixi76 – 794Combined sources
Beta strandi82 – 887Combined sources
Helixi92 – 10110Combined sources
Beta strandi105 – 1139Combined sources
Helixi118 – 1247Combined sources
Beta strandi128 – 1314Combined sources
Helixi135 – 14713Combined sources
Helixi149 – 1557Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1OX7X-ray1.43A/B1-158[»]
1P6OX-ray1.14A/B1-158[»]
1RB7X-ray2.10A/B1-158[»]
1UAQX-ray1.60A/B1-158[»]
1YSBX-ray1.70A/B1-158[»]
1YSDX-ray1.90A/B1-158[»]
2O3KX-ray2.30A/B1-158[»]
ProteinModelPortaliQ12178.
SMRiQ12178. Positions 1-158.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ12178.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini9 – 129121CMP/dCMP-type deaminasePROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi129 – 1324Poly-Val

Sequence similaritiesi

Contains 1 CMP/dCMP-type deaminase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0590.
HOGENOMiHOG000085050.
InParanoidiQ12178.
KOiK01485.
OMAiYGIPRVV.
OrthoDBiEOG7R2BX1.

Family and domain databases

InterProiIPR016192. APOBEC/CMP_deaminase_Zn-bd.
IPR002125. CMP_dCMP_Zn-bd.
IPR016193. Cytidine_deaminase-like.
[Graphical view]
PfamiPF00383. dCMP_cyt_deam_1. 1 hit.
[Graphical view]
SUPFAMiSSF53927. SSF53927. 1 hit.
PROSITEiPS00903. CYT_DCMP_DEAMINASES_1. 1 hit.
PS51747. CYT_DCMP_DEAMINASES_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q12178-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVTGGMASKW DQKGMDIAYE EAALGYKEGG VPIGGCLINN KDGSVLGRGH
60 70 80 90 100
NMRFQKGSAT LHGEISTLEN CGRLEGKVYK DTTLYTTLSP CDMCTGAIIM
110 120 130 140 150
YGIPRCVVGE NVNFKSKGEK YLQTRGHEVV VVDDERCKKI MKQFIDERPQ

DWFEDIGE
Length:158
Mass (Da):17,507
Last modified:October 31, 1996 - v1
Checksum:i19DB41E9AF26ADDC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U55193 Genomic DNA. Translation: AAC13409.1.
AF005261 Genomic DNA. Translation: AAB67713.1.
Z71255 Genomic DNA. Translation: CAA95006.1.
Z49219 Genomic DNA. Translation: CAA89179.1.
BK006949 Genomic DNA. Translation: DAA11483.1.
PIRiS54083.
RefSeqiNP_015387.1. NM_001184159.1.

Genome annotation databases

EnsemblFungiiYPR062W; YPR062W; YPR062W.
GeneIDi856175.
KEGGisce:YPR062W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U55193 Genomic DNA. Translation: AAC13409.1.
AF005261 Genomic DNA. Translation: AAB67713.1.
Z71255 Genomic DNA. Translation: CAA95006.1.
Z49219 Genomic DNA. Translation: CAA89179.1.
BK006949 Genomic DNA. Translation: DAA11483.1.
PIRiS54083.
RefSeqiNP_015387.1. NM_001184159.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1OX7X-ray1.43A/B1-158[»]
1P6OX-ray1.14A/B1-158[»]
1RB7X-ray2.10A/B1-158[»]
1UAQX-ray1.60A/B1-158[»]
1YSBX-ray1.70A/B1-158[»]
1YSDX-ray1.90A/B1-158[»]
2O3KX-ray2.30A/B1-158[»]
ProteinModelPortaliQ12178.
SMRiQ12178. Positions 1-158.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36235. 25 interactions.
DIPiDIP-1662N.
IntActiQ12178. 2 interactions.
MINTiMINT-392713.
STRINGi4932.YPR062W.

Proteomic databases

MaxQBiQ12178.
PaxDbiQ12178.
PeptideAtlasiQ12178.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYPR062W; YPR062W; YPR062W.
GeneIDi856175.
KEGGisce:YPR062W.

Organism-specific databases

CYGDiYPR062w.
SGDiS000006266. FCY1.

Phylogenomic databases

eggNOGiCOG0590.
HOGENOMiHOG000085050.
InParanoidiQ12178.
KOiK01485.
OMAiYGIPRVV.
OrthoDBiEOG7R2BX1.

Enzyme and pathway databases

UniPathwayiUPA00574; UER00635.
BioCyciMetaCyc:YPR062W-MONOMER.
YEAST:YPR062W-MONOMER.
BRENDAi3.5.4.1. 984.

Miscellaneous databases

EvolutionaryTraceiQ12178.
NextBioi981337.

Gene expression databases

GenevestigatoriQ12178.

Family and domain databases

InterProiIPR016192. APOBEC/CMP_deaminase_Zn-bd.
IPR002125. CMP_dCMP_Zn-bd.
IPR016193. Cytidine_deaminase-like.
[Graphical view]
PfamiPF00383. dCMP_cyt_deam_1. 1 hit.
[Graphical view]
SUPFAMiSSF53927. SSF53927. 1 hit.
PROSITEiPS00903. CYT_DCMP_DEAMINASES_1. 1 hit.
PS51747. CYT_DCMP_DEAMINASES_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Characterization of the Saccharomyces cerevisiae FCY1 gene encoding cytosine deaminase and its homologue FCA1 of Candida albicans."
    Erbs P., Exinger F., Jund R.
    Curr. Genet. 31:1-6(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 28383 / FL100 / VTT C-80102.
  2. "Isolation of thermally stable cytosine deaminase from baker's yeast."
    Senter P.D., Su P.C.D., Marquardt H., Hayden M.S., Linsley P.S.
    Submitted (JUL-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 334.
  3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI."
    Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M.
    , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J.
    Nature 387:103-105(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  6. "Crystal structure of yeast cytosine deaminase. Insights into enzyme mechanism and evolution."
    Ko T.-P., Lin J.-J., Hu C.-Y., Hsu Y.-H., Wang A.H.-J., Liaw S.-H.
    J. Biol. Chem. 278:19111-19117(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.6 ANGSTROMS).
  7. "The 1.14 A crystal structure of yeast cytosine deaminase: evolution of nucleotide salvage enzymes and implications for genetic chemotherapy."
    Ireton G.C., Black M.E., Stoddard B.L.
    Structure 11:961-972(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.14 ANGSTROMS) IN COMPLEX WITH SUBSTRATE ANALOG AND ZINC IONS, SUBUNIT.

Entry informationi

Entry nameiFCY1_YEAST
AccessioniPrimary (citable) accession number: Q12178
Secondary accession number(s): D6W467
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 31, 1997
Last sequence update: October 31, 1996
Last modified: March 31, 2015
This is version 134 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 5180 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  5. Yeast chromosome XVI
    Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.