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Protein

Cytosine deaminase

Gene

FCY1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Converts cytosine to uracil or 5-methylcytosine to thymine by deaminating carbon number 4.

Miscellaneous

Present with 5180 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

Cytosine + H2O = uracil + NH3.

Cofactori

Zn2+By similarity

Pathwayi: UMP biosynthesis via salvage pathway

This protein is involved in step 1 of the subpathway that synthesizes uracil from cytosine.
Proteins known to be involved in this subpathway in this organism are:
  1. Cytosine deaminase (FCY1)
This subpathway is part of the pathway UMP biosynthesis via salvage pathway, which is itself part of Pyrimidine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes uracil from cytosine, the pathway UMP biosynthesis via salvage pathway and in Pyrimidine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei51Substrate1
Metal bindingi62Zinc; catalytic1
Active sitei64Proton donor1
Metal bindingi91Zinc; catalytic1
Metal bindingi94Zinc; catalytic1
Binding sitei155Substrate1

GO - Molecular functioni

GO - Biological processi

  • cytidine metabolic process Source: SGD
  • cytosine metabolic process Source: SGD
  • pyrimidine-containing compound salvage Source: SGD
  • RNA modification Source: GO_Central
  • UMP salvage Source: UniProtKB-UniPathway

Keywordsi

Molecular functionHydrolase
LigandMetal-binding, Zinc

Enzyme and pathway databases

BioCyciMetaCyc:YPR062W-MONOMER
YEAST:YPR062W-MONOMER
BRENDAi3.5.4.1 984
UniPathwayiUPA00574; UER00635

Names & Taxonomyi

Protein namesi
Recommended name:
Cytosine deaminase (EC:3.5.4.1)
Alternative name(s):
Cytosine aminohydrolase
Gene namesi
Name:FCY1
Ordered Locus Names:YPR062W
ORF Names:YP9499.17
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XVI

Organism-specific databases

EuPathDBiFungiDB:YPR062W
SGDiS000006266 FCY1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001717021 – 158Cytosine deaminaseAdd BLAST158

Proteomic databases

MaxQBiQ12178
PaxDbiQ12178
PRIDEiQ12178

Interactioni

Subunit structurei

Homodimer.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
HSP104P315392EBI-6851,EBI-8050

Protein-protein interaction databases

BioGridi36235, 60 interactors
DIPiDIP-1662N
IntActiQ12178, 13 interactors
MINTiQ12178
STRINGi4932.YPR062W

Structurei

Secondary structure

1158
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi11 – 27Combined sources17
Beta strandi34 – 39Combined sources6
Turni40 – 42Combined sources3
Beta strandi45 – 50Combined sources6
Helixi53 – 56Combined sources4
Helixi63 – 71Combined sources9
Helixi76 – 79Combined sources4
Beta strandi82 – 88Combined sources7
Helixi92 – 101Combined sources10
Beta strandi105 – 113Combined sources9
Helixi118 – 124Combined sources7
Beta strandi128 – 131Combined sources4
Helixi135 – 147Combined sources13
Helixi149 – 155Combined sources7

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1OX7X-ray1.43A/B1-158[»]
1P6OX-ray1.14A/B1-158[»]
1RB7X-ray2.10A/B1-158[»]
1UAQX-ray1.60A/B1-158[»]
1YSBX-ray1.70A/B1-158[»]
1YSDX-ray1.90A/B1-158[»]
2O3KX-ray2.30A/B1-158[»]
ProteinModelPortaliQ12178
SMRiQ12178
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ12178

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini9 – 129CMP/dCMP-type deaminasePROSITE-ProRule annotationAdd BLAST121

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi129 – 132Poly-Val4

Sequence similaritiesi

Phylogenomic databases

HOGENOMiHOG000085050
InParanoidiQ12178
KOiK01485
OMAiILLYKIP
OrthoDBiEOG092C4YNP

Family and domain databases

InterProiView protein in InterPro
IPR016192 APOBEC/CMP_deaminase_Zn-bd
IPR002125 CMP_dCMP_dom
IPR016193 Cytidine_deaminase-like
PfamiView protein in Pfam
PF00383 dCMP_cyt_deam_1, 1 hit
SUPFAMiSSF53927 SSF53927, 1 hit
PROSITEiView protein in PROSITE
PS00903 CYT_DCMP_DEAMINASES_1, 1 hit
PS51747 CYT_DCMP_DEAMINASES_2, 1 hit

Sequencei

Sequence statusi: Complete.

Q12178-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVTGGMASKW DQKGMDIAYE EAALGYKEGG VPIGGCLINN KDGSVLGRGH
60 70 80 90 100
NMRFQKGSAT LHGEISTLEN CGRLEGKVYK DTTLYTTLSP CDMCTGAIIM
110 120 130 140 150
YGIPRCVVGE NVNFKSKGEK YLQTRGHEVV VVDDERCKKI MKQFIDERPQ

DWFEDIGE
Length:158
Mass (Da):17,507
Last modified:November 1, 1996 - v1
Checksum:i19DB41E9AF26ADDC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U55193 Genomic DNA Translation: AAC13409.1
AF005261 Genomic DNA Translation: AAB67713.1
Z71255 Genomic DNA Translation: CAA95006.1
Z49219 Genomic DNA Translation: CAA89179.1
BK006949 Genomic DNA Translation: DAA11483.1
PIRiS54083
RefSeqiNP_015387.1, NM_001184159.1

Genome annotation databases

EnsemblFungiiYPR062W; YPR062W; YPR062W
GeneIDi856175
KEGGisce:YPR062W

Similar proteinsi

Entry informationi

Entry nameiFCY1_YEAST
AccessioniPrimary (citable) accession number: Q12178
Secondary accession number(s): D6W467
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: March 28, 2018
This is version 159 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome
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Main funding by: National Institutes of Health