Reviewed, UniProtKB/Swiss-Prot Q12178 (FCY1_YEAST)

Names and origin

Protein names

Recommended name:
    Cytosine deaminase
    EC=3.5.4.1
Alternative name(s):
    Cytosine aminohydrolase

Gene names

Name:	FCY1
Ordered Locus Names:	YPR062W
ORF Names:	YP9499.17

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	158 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Converts cytosine to uracil or 5-methylcytosine to thymine by deaminating carbon number 4.
Catalytic activity	Cytosine + H2O = uracil + NH3.
Cofactor	Zinc By similarity.
Pathway	Pyrimidine metabolism; UMP biosynthesis via salvage pathway; uracil from cytosine: step 1/1.
Subunit structure	Homodimer. Ref.6
Miscellaneous	Present with 5180 molecules/cell in log phase SD medium. Ref.4
Sequence similarities	Belongs to the cytidine and deoxycytidylate deaminase family.

Ontologies

Keywords
Ligand	Metal-binding Zinc
Molecular function	Hydrolase
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	cytidine metabolic process Inferred from mutant phenotype. Source: SGD cytosine metabolic process Ref.1 Inferred from mutant phenotype. Source: SGD pyrimidine salvage Ref.1 Inferred from mutant phenotype. Source: SGD
Cellular component	cytoplasm Inferred from direct assay. Source: SGD nucleus Inferred from direct assay. Source: SGD
Molecular function	cytosine deaminase activity Ref.1 Inferred from direct assay. Source: SGD identical protein binding Inferred from physical interaction. Source: IntAct zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
itself		1	EBI-6851,EBI-6851
	P53751	1	EBI-6851,EBI-28557
RNR2	P09938	1	EBI-6851,EBI-15240

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 158

158

Cytosine deaminase

PRO_0000171702

Regions

Compositional bias

129 – 132

4

Poly-Val

Sites

Active site

64

1

Proton donor

Metal binding

62

1

Zinc; catalytic

Metal binding

91

1

Zinc; catalytic

Metal binding

94

1

Zinc; catalytic

Binding site

51

1

Substrate

Binding site

155

1

Substrate

Secondary structure

1

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158

Helix Strand Turn

Details...

Helix

11 – 27

17

Beta strand

34 – 39

6

Turn

40 – 42

3

Beta strand

45 – 50

6

Helix

53 – 56

4

Helix

63 – 71

9

Helix

76 – 79

4

Beta strand

82 – 88

7

Helix

92 – 101

10

Beta strand

105 – 113

9

Helix

118 – 124

7

Beta strand

128 – 131

4

Helix

135 – 147

13

Helix

149 – 155

7

Sequences

	Sequence		Length	Mass (Da)
	Q12178-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 19DB41E9AF26ADDC	FASTA	158	17,507
	10 20 30 40 50 60 MVTGGMASKW DQKGMDIAYE EAALGYKEGG VPIGGCLINN KDGSVLGRGH NMRFQKGSAT 70 80 90 100 110 120 LHGEISTLEN CGRLEGKVYK DTTLYTTLSP CDMCTGAIIM YGIPRCVVGE NVNFKSKGEK 130 140 150 YLQTRGHEVV VVDDERCKKI MKQFIDERPQ DWFEDIGE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization of the Saccharomyces cerevisiae FCY1 gene encoding cytosine deaminase and its homologue FCA1 of Candida albicans." Erbs P., Exinger F., Jund R. Curr. Genet. 31:1-6(1997) [PubMed: 9000374] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 28383 / FL100 / VTT C-80102.
[2]	"Isolation of thermally stable cytosine deaminase from baker's yeast." Senter P.D., Su P.C.D., Marquardt H., Hayden M.S., Linsley P.S. Submitted (AUG-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 334.
[3]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI." Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M. , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J. Nature 387:103-105(1997) [PubMed: 9169875] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[4]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[5]	"Crystal structure of yeast cytosine deaminase. Insights into enzyme mechanism and evolution." Ko T.-P., Lin J.-J., Hu C.-Y., Hsu Y.-H., Wang A.H.-J., Liaw S.-H. J. Biol. Chem. 278:19111-19117(2003) [PubMed: 12637534] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.6 ANGSTROMS).
[6]	"The 1.14 A crystal structure of yeast cytosine deaminase: evolution of nucleotide salvage enzymes and implications for genetic chemotherapy." Ireton G.C., Black M.E., Stoddard B.L. Structure 11:961-972(2003) [PubMed: 12906827] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.14 ANGSTROMS) IN COMPLEX WITH SUBSTRATE ANALOG AND ZINC IONS, SUBUNIT.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

U55193 Genomic DNA. Translation: AAC13409.1.
AF005261 Genomic DNA. Translation: AAB67713.1.
Z71255 Genomic DNA. Translation: CAA95006.1.
Z49219 Genomic DNA. Translation: CAA89179.1.

PIR

S54083.

RefSeq

NP_015387.1.

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1OX7	X-ray	1.43	A/B	1-158	[»]
1P6O	X-ray	1.14	A/B	1-158	[»]
1RB7	X-ray	2.10	A/B	1-158	[»]
1UAQ	X-ray	1.60	A/B	1-158	[»]
1YSB	X-ray	1.70	A/B	1-158	[»]
1YSD	X-ray	1.90	A/B	1-158	[»]
2O3K	X-ray	2.30	A/B	1-158	[»]

ModBase

Search...

Protein-protein interaction databases

DIP

DIP:1662N.

IntAct

Q12178. 4 interactions.

Proteomic databases

PeptideAtlas

Q12178.

Genome annotation databases

Ensembl

YPR062W. Saccharomyces cerevisiae. [Contig view]

GeneID

856175.

GenomeReviews

Gene locus YPR062W in contig U00094_GR.

KEGG

sce:YPR062W.

NMPDR

fig|4932.3.peg.6523.

Organism-specific databases

CYGD

YPR062w.

SGD

S000006266. FCY1.

Yeast-GFP

Search...

Phylogenomic databases

HOGENOM

Q12178.

OMA

Q12178. EMDCLEN.

Enzyme and pathway databases

BRENDA

3.5.4.1. 250.

Gene expression databases

ArrayExpress

Q12178.

GermOnline

YPR062W. Saccharomyces cerevisiae.

Family and domain databases

InterPro

IPR016192. APOBEC/CMP_deaminase_Zn-bd.
IPR002125. CMP_dCMP_Zn_bd.
[Graphical view]

Pfam

PF00383. dCMP_cyt_deam_1. 1 hit.
[Graphical view]

PROSITE

PS00903. CYT_DCMP_DEAMINASES. 1 hit.
[Graphical view]

ProtoNet

Search...

Other Resources

NextBio

981337.

Entry information

Entry name

FCY1_YEAST

Accession

Primary (citable) accession number: Q12178

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1996
Last modified:	June 16, 2009
This is version 87 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)