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Q12178 (FCY1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 129. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Cytosine deaminase

EC=3.5.4.1
Alternative name(s):
Cytosine aminohydrolase
Gene names
Name:FCY1
Ordered Locus Names:YPR062W
ORF Names:YP9499.17
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length158 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Converts cytosine to uracil or 5-methylcytosine to thymine by deaminating carbon number 4.

Catalytic activity

Cytosine + H2O = uracil + NH3.

Cofactor

Zinc By similarity.

Pathway

Pyrimidine metabolism; UMP biosynthesis via salvage pathway; uracil from cytosine: step 1/1.

Subunit structure

Homodimer. Ref.7

Miscellaneous

Present with 5180 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the cytidine and deoxycytidylate deaminase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 158158Cytosine deaminase
PRO_0000171702

Regions

Compositional bias129 – 1324Poly-Val

Sites

Active site641Proton donor
Metal binding621Zinc; catalytic
Metal binding911Zinc; catalytic
Metal binding941Zinc; catalytic
Binding site511Substrate
Binding site1551Substrate

Secondary structure

............................ 158
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q12178 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 19DB41E9AF26ADDC

FASTA15817,507
        10         20         30         40         50         60 
MVTGGMASKW DQKGMDIAYE EAALGYKEGG VPIGGCLINN KDGSVLGRGH NMRFQKGSAT 

        70         80         90        100        110        120 
LHGEISTLEN CGRLEGKVYK DTTLYTTLSP CDMCTGAIIM YGIPRCVVGE NVNFKSKGEK 

       130        140        150 
YLQTRGHEVV VVDDERCKKI MKQFIDERPQ DWFEDIGE 

« Hide

References

« Hide 'large scale' references
[1]"Characterization of the Saccharomyces cerevisiae FCY1 gene encoding cytosine deaminase and its homologue FCA1 of Candida albicans."
Erbs P., Exinger F., Jund R.
Curr. Genet. 31:1-6(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 28383 / FL100 / VTT C-80102.
[2]"Isolation of thermally stable cytosine deaminase from baker's yeast."
Senter P.D., Su P.C.D., Marquardt H., Hayden M.S., Linsley P.S.
Submitted (AUG-1997) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 334.
[3]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI."
Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M. expand/collapse author list , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J.
Nature 387:103-105(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[5]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]"Crystal structure of yeast cytosine deaminase. Insights into enzyme mechanism and evolution."
Ko T.-P., Lin J.-J., Hu C.-Y., Hsu Y.-H., Wang A.H.-J., Liaw S.-H.
J. Biol. Chem. 278:19111-19117(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.6 ANGSTROMS).
[7]"The 1.14 A crystal structure of yeast cytosine deaminase: evolution of nucleotide salvage enzymes and implications for genetic chemotherapy."
Ireton G.C., Black M.E., Stoddard B.L.
Structure 11:961-972(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.14 ANGSTROMS) IN COMPLEX WITH SUBSTRATE ANALOG AND ZINC IONS, SUBUNIT.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U55193 Genomic DNA. Translation: AAC13409.1.
AF005261 Genomic DNA. Translation: AAB67713.1.
Z71255 Genomic DNA. Translation: CAA95006.1.
Z49219 Genomic DNA. Translation: CAA89179.1.
BK006949 Genomic DNA. Translation: DAA11483.1.
PIRS54083.
RefSeqNP_015387.1. NM_001184159.1.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1OX7X-ray1.43A/B1-158[»]
1P6OX-ray1.14A/B1-158[»]
1RB7X-ray2.10A/B1-158[»]
1UAQX-ray1.60A/B1-158[»]
1YSBX-ray1.70A/B1-158[»]
1YSDX-ray1.90A/B1-158[»]
2O3KX-ray2.30A/B1-158[»]
ProteinModelPortalQ12178.
SMRQ12178. Positions 1-158.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid36235. 25 interactions.
DIPDIP-1662N.
IntActQ12178. 2 interactions.
MINTMINT-392713.
STRING4932.YPR062W.

Proteomic databases

MaxQBQ12178.
PaxDbQ12178.
PeptideAtlasQ12178.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYPR062W; YPR062W; YPR062W.
GeneID856175.
KEGGsce:YPR062W.

Organism-specific databases

CYGDYPR062w.
SGDS000006266. FCY1.

Phylogenomic databases

eggNOGCOG0590.
HOGENOMHOG000085050.
KOK01485.
OMARININAN.
OrthoDBEOG7R2BX1.

Enzyme and pathway databases

BioCycMetaCyc:YPR062W-MONOMER.
YEAST:YPR062W-MONOMER.
UniPathwayUPA00574; UER00635.

Gene expression databases

GenevestigatorQ12178.

Family and domain databases

InterProIPR016192. APOBEC/CMP_deaminase_Zn-bd.
IPR002125. CMP_dCMP_Zn-bd.
IPR016193. Cytidine_deaminase-like.
[Graphical view]
PfamPF00383. dCMP_cyt_deam_1. 1 hit.
[Graphical view]
SUPFAMSSF53927. SSF53927. 1 hit.
PROSITEPS00903. CYT_DCMP_DEAMINASES. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceQ12178.
NextBio981337.

Entry information

Entry nameFCY1_YEAST
AccessionPrimary (citable) accession number: Q12178
Secondary accession number(s): D6W467
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: May 14, 2014
This is version 129 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XVI

Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

PATHWAY comments

Index of metabolic and biosynthesis pathways