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Protein

Ribosome biogenesis protein MAK21

Gene

MAK21

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Required for 60S ribosomal subunit synthesis.1 Publication

GO - Molecular functioni

  • identical protein binding Source: IntAct

GO - Biological processi

  • ribosomal large subunit assembly Source: SGD
  • ribosomal large subunit biogenesis Source: SGD
Complete GO annotation...

Keywords - Biological processi

Ribosome biogenesis

Enzyme and pathway databases

BioCyciYEAST:G3O-29668-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribosome biogenesis protein MAK21
Alternative name(s):
Maintenance of killer protein 21
Nucleolar complex protein 1
Gene namesi
Name:MAK21
Synonyms:NOC1
Ordered Locus Names:YDR060W
ORF Names:D4237, YD9609.14
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

EuPathDBiFungiDB:YDR060W.
SGDiS000002467. MAK21.

Subcellular locationi

GO - Cellular componenti

  • Noc1p-Noc2p complex Source: SGD
  • nucleolus Source: UniProtKB-SubCell
  • preribosome, large subunit precursor Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedCombined sources
Chaini2 – 10251024Ribosome biogenesis protein MAK21PRO_0000173472Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserineCombined sources
Modified residuei62 – 621PhosphoserineCombined sources
Modified residuei73 – 731PhosphoserineCombined sources
Modified residuei80 – 801PhosphoserineCombined sources
Modified residuei275 – 2751PhosphoserineCombined sources
Modified residuei708 – 7081PhosphotyrosineCombined sources
Modified residuei710 – 7101PhosphoserineCombined sources
Modified residuei874 – 8741PhosphoserineCombined sources
Modified residuei878 – 8781PhosphoserineCombined sources
Modified residuei977 – 9771PhosphoserineCombined sources
Modified residuei978 – 9781PhosphoserineCombined sources
Modified residuei1024 – 10241PhosphoserineCombined sources

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiQ12176.
PeptideAtlasiQ12176.

PTM databases

iPTMnetiQ12176.

Interactioni

Subunit structurei

Interacts with NOC2. Forms a nucleolar complex with NOC2 that binds to 90S and 66S pre-ribosomes.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
itself3EBI-10944,EBI-10944
DBP10Q123893EBI-10944,EBI-5644
HAS1Q035324EBI-10944,EBI-8170
LOC1P435863EBI-10944,EBI-22906
NOC2P397449EBI-10944,EBI-29259
NOG1Q028923EBI-10944,EBI-12105
NOG2P537423EBI-10944,EBI-28532
NOP4P378388EBI-10944,EBI-12122
RLP7P406934EBI-10944,EBI-15415
RPF2P361606EBI-10944,EBI-15881
RRP12Q127544EBI-10944,EBI-30678
RRP15Q065114EBI-10944,EBI-34602
RRP5Q050226EBI-10944,EBI-16011

GO - Molecular functioni

  • identical protein binding Source: IntAct

Protein-protein interaction databases

BioGridi32115. 183 interactions.
DIPiDIP-4040N.
IntActiQ12176. 102 interactions.
MINTiMINT-563761.

Structurei

3D structure databases

ProteinModelPortaliQ12176.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi74 – 774Poly-Asp
Compositional biasi117 – 1226Poly-Asp
Compositional biasi979 – 9868Poly-Glu

Sequence similaritiesi

Belongs to the CBF/MAK21 family.Curated

Phylogenomic databases

GeneTreeiENSGT00390000006395.
HOGENOMiHOG000207540.
InParanoidiQ12176.
KOiK14832.
OMAiPHIAAGC.
OrthoDBiEOG7P02SB.

Family and domain databases

InterProiIPR016024. ARM-type_fold.
IPR005612. CCAAT-binding_factor.
[Graphical view]
PfamiPF03914. CBF. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 3 hits.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q12176-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSENNGNPLD LSSLRNKISS KLRDNNSKKA KKTHKGKDVK ASSNSKKVNE
60 70 80 90 100
DIRREALALG ASEEDLKLIQ GLSDDDDAKS EQEFDAVADE DADDKGFKND
110 120 130 140 150
LQNFMKNVGF DQHKLEDVDD DDIEEESTSS KESKIPAQEK EHAQSNIASS
160 170 180 190 200
TIEKTSQESI DNGSEQEENT VEEANLSSDQ EPESESAEKE KKEEKDGGLI
210 220 230 240 250
TQTTIISSDK LIIPYDKPWY EIPLDPQVGQ NDDVEELSKE QIEKLFERGK
260 270 280 290 300
QTLEADNQTY YEEFTKDSSQ AKFMSQILSD GTLNDKISAV TLLIQDSPLH
310 320 330 340 350
NTKSLETLVS YCGKKSRNSA LQSLNALKDL FLNGLLPNRK LRYFKNQPGL
360 370 380 390 400
SMMLNKKTLA IFYFEDYLKK LFFRVLEVLE VLSHDPIIHV RLQILNHVFD
410 420 430 440 450
LLTNQPEQEF NLLRLGVNKI GDIDSKVSSK ASYLLLKLEQ AHPNMKSIVI
460 470 480 490 500
DAIVDIALRP NADYHTTYYS VITLNQTILK RSEDSVANKL VKTYFTLFEK
510 520 530 540 550
FLINTDKDNT NGVVKSNSKS YEEKRKKNFK KGKHGGKSVK IEKTENEVLD
560 570 580 590 600
EKNSKLFSAL LTGINRAFPF AQIPASVYEV HMETLFKITH SSNFNTSIQA
610 620 630 640 650
LVLINQVTVK AKLNSDRYYR TLYESLFDPR LVNSSKQGIY LNLLYKSLKQ
660 670 680 690 700
DALNVERVEA FVKRILQVCS HWLNVGTITG FFFLLIQLAK TVPQIKNLLT
710 720 730 740 750
NTPVDYEYES DAEEEQGDKD IKRKEYDGRK RDPKFANAEK SSLWEINNFI
760 770 780 790 800
NHFHPTVKTY ANAYVTGETE QIAKPDLGLF TLSHFLDRFV YRSAKQTNTA
810 820 830 840 850
RGTSIMQPLF SGSRVNDSVL VKASDIMHDQ GPVNTEDWLT KKVEDIKPED
860 870 880 890 900
KFFYQYFTTK KTADGKGKKS NKASNFDSDD EMDENEIWSA LVKSRPDVED
910 920 930 940 950
DSDDSELDFA EDDFSDSTSD DEPKLDAIDD EDAKSEGSQE SDQEEGLDED
960 970 980 990 1000
IFYSFDGEQD NSDKKRSFAE SSEEDESSEE EKEEEENKEV SAKRAKKKQR
1010 1020
KNMLKSLPVF ASADDYAQYL DQDSD
Length:1,025
Mass (Da):116,677
Last modified:November 1, 1996 - v1
Checksum:iFB80378727ED71D8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X84162 Genomic DNA. Translation: CAA58976.1.
Z74356 Genomic DNA. Translation: CAA98878.1.
Z49209 Genomic DNA. Translation: CAA89089.1.
BK006938 Genomic DNA. Translation: DAA11906.1.
PIRiS54044.
RefSeqiNP_010345.3. NM_001180368.3.

Genome annotation databases

EnsemblFungiiYDR060W; YDR060W; YDR060W.
GeneIDi851632.
KEGGisce:YDR060W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X84162 Genomic DNA. Translation: CAA58976.1.
Z74356 Genomic DNA. Translation: CAA98878.1.
Z49209 Genomic DNA. Translation: CAA89089.1.
BK006938 Genomic DNA. Translation: DAA11906.1.
PIRiS54044.
RefSeqiNP_010345.3. NM_001180368.3.

3D structure databases

ProteinModelPortaliQ12176.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi32115. 183 interactions.
DIPiDIP-4040N.
IntActiQ12176. 102 interactions.
MINTiMINT-563761.

PTM databases

iPTMnetiQ12176.

Proteomic databases

MaxQBiQ12176.
PeptideAtlasiQ12176.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYDR060W; YDR060W; YDR060W.
GeneIDi851632.
KEGGisce:YDR060W.

Organism-specific databases

EuPathDBiFungiDB:YDR060W.
SGDiS000002467. MAK21.

Phylogenomic databases

GeneTreeiENSGT00390000006395.
HOGENOMiHOG000207540.
InParanoidiQ12176.
KOiK14832.
OMAiPHIAAGC.
OrthoDBiEOG7P02SB.

Enzyme and pathway databases

BioCyciYEAST:G3O-29668-MONOMER.

Miscellaneous databases

NextBioi969182.
PROiQ12176.

Family and domain databases

InterProiIPR016024. ARM-type_fold.
IPR005612. CCAAT-binding_factor.
[Graphical view]
PfamiPF03914. CBF. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 3 hits.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Nucleotide sequence analysis of a 32,500 bp region of the right arm of Saccharomyces cerevisiae chromosome IV."
    Brandt P., Ramlow S., Otto B., Bloecker H.
    Yeast 12:85-90(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV."
    Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T.
    , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P.
    Nature 387:75-78(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "Mak21p of Saccharomyces cerevisiae, a homolog of human CAATT-binding protein, is essential for 60 S ribosomal subunit biogenesis."
    Edskes H.K., Ohtake Y., Wickner R.B.
    J. Biol. Chem. 273:28912-28920(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
  5. "Maturation and intranuclear transport of pre-ribosomes requires Noc proteins."
    Milkereit P., Gadal O., Podtelejnikov A., Trumtel S., Gas N., Petfalski E., Tollervey D., Mann M., Hurt E., Tschochner H.
    Cell 105:499-509(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH NOC2.
  6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  7. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-62; SER-73; SER-977 AND SER-978, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  8. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-62; SER-73; SER-80; TYR-708; SER-710 AND SER-878, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  9. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-73; SER-275; SER-710; SER-874; SER-878 AND SER-1024, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  10. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS], IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiMAK21_YEAST
AccessioniPrimary (citable) accession number: Q12176
Secondary accession number(s): D6VS46
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: November 1, 1996
Last modified: May 11, 2016
This is version 137 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 15000 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.