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Protein

Ribosome biogenesis protein MAK21

Gene

MAK21

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Required for 60S ribosomal subunit synthesis.1 Publication

GO - Biological processi

  • ribosomal large subunit assembly Source: SGD
  • ribosomal large subunit biogenesis Source: SGD
Complete GO annotation...

Keywords - Biological processi

Ribosome biogenesis

Enzyme and pathway databases

BioCyciYEAST:G3O-29668-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribosome biogenesis protein MAK21
Alternative name(s):
Maintenance of killer protein 21
Nucleolar complex protein 1
Gene namesi
Name:MAK21
Synonyms:NOC1
Ordered Locus Names:YDR060W
ORF Names:D4237, YD9609.14
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

EuPathDBiFungiDB:YDR060W.
SGDiS000002467. MAK21.

Subcellular locationi

GO - Cellular componenti

  • Noc1p-Noc2p complex Source: SGD
  • nucleolus Source: UniProtKB-SubCell
  • preribosome, large subunit precursor Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00001734722 – 1025Ribosome biogenesis protein MAK21Add BLAST1024

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineCombined sources1
Modified residuei62PhosphoserineCombined sources1
Modified residuei73PhosphoserineCombined sources1
Modified residuei80PhosphoserineCombined sources1
Modified residuei275PhosphoserineCombined sources1
Modified residuei708PhosphotyrosineCombined sources1
Modified residuei710PhosphoserineCombined sources1
Modified residuei874PhosphoserineCombined sources1
Modified residuei878PhosphoserineCombined sources1
Modified residuei977PhosphoserineCombined sources1
Modified residuei978PhosphoserineCombined sources1
Modified residuei1024PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiQ12176.
PRIDEiQ12176.

PTM databases

iPTMnetiQ12176.

Interactioni

Subunit structurei

Interacts with NOC2. Forms a nucleolar complex with NOC2 that binds to 90S and 66S pre-ribosomes.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
itself3EBI-10944,EBI-10944
DBP10Q123893EBI-10944,EBI-5644
HAS1Q035324EBI-10944,EBI-8170
LOC1P435863EBI-10944,EBI-22906
NOC2P397449EBI-10944,EBI-29259
NOG1Q028923EBI-10944,EBI-12105
NOG2P537423EBI-10944,EBI-28532
NOP4P378388EBI-10944,EBI-12122
RLP7P406934EBI-10944,EBI-15415
RPF2P361606EBI-10944,EBI-15881
RRP12Q127544EBI-10944,EBI-30678
RRP15Q065114EBI-10944,EBI-34602
RRP5Q050226EBI-10944,EBI-16011

Protein-protein interaction databases

BioGridi32115. 184 interactors.
DIPiDIP-4040N.
IntActiQ12176. 103 interactors.
MINTiMINT-563761.

Structurei

3D structure databases

ProteinModelPortaliQ12176.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi74 – 77Poly-Asp4
Compositional biasi117 – 122Poly-Asp6
Compositional biasi979 – 986Poly-Glu8

Sequence similaritiesi

Belongs to the CBF/MAK21 family.Curated

Phylogenomic databases

GeneTreeiENSGT00390000006395.
HOGENOMiHOG000207540.
InParanoidiQ12176.
KOiK14832.
OMAiYKKVAVK.
OrthoDBiEOG092C13JZ.

Family and domain databases

InterProiIPR016024. ARM-type_fold.
IPR005612. CCAAT-binding_factor.
[Graphical view]
PfamiPF03914. CBF. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 3 hits.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q12176-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSENNGNPLD LSSLRNKISS KLRDNNSKKA KKTHKGKDVK ASSNSKKVNE
60 70 80 90 100
DIRREALALG ASEEDLKLIQ GLSDDDDAKS EQEFDAVADE DADDKGFKND
110 120 130 140 150
LQNFMKNVGF DQHKLEDVDD DDIEEESTSS KESKIPAQEK EHAQSNIASS
160 170 180 190 200
TIEKTSQESI DNGSEQEENT VEEANLSSDQ EPESESAEKE KKEEKDGGLI
210 220 230 240 250
TQTTIISSDK LIIPYDKPWY EIPLDPQVGQ NDDVEELSKE QIEKLFERGK
260 270 280 290 300
QTLEADNQTY YEEFTKDSSQ AKFMSQILSD GTLNDKISAV TLLIQDSPLH
310 320 330 340 350
NTKSLETLVS YCGKKSRNSA LQSLNALKDL FLNGLLPNRK LRYFKNQPGL
360 370 380 390 400
SMMLNKKTLA IFYFEDYLKK LFFRVLEVLE VLSHDPIIHV RLQILNHVFD
410 420 430 440 450
LLTNQPEQEF NLLRLGVNKI GDIDSKVSSK ASYLLLKLEQ AHPNMKSIVI
460 470 480 490 500
DAIVDIALRP NADYHTTYYS VITLNQTILK RSEDSVANKL VKTYFTLFEK
510 520 530 540 550
FLINTDKDNT NGVVKSNSKS YEEKRKKNFK KGKHGGKSVK IEKTENEVLD
560 570 580 590 600
EKNSKLFSAL LTGINRAFPF AQIPASVYEV HMETLFKITH SSNFNTSIQA
610 620 630 640 650
LVLINQVTVK AKLNSDRYYR TLYESLFDPR LVNSSKQGIY LNLLYKSLKQ
660 670 680 690 700
DALNVERVEA FVKRILQVCS HWLNVGTITG FFFLLIQLAK TVPQIKNLLT
710 720 730 740 750
NTPVDYEYES DAEEEQGDKD IKRKEYDGRK RDPKFANAEK SSLWEINNFI
760 770 780 790 800
NHFHPTVKTY ANAYVTGETE QIAKPDLGLF TLSHFLDRFV YRSAKQTNTA
810 820 830 840 850
RGTSIMQPLF SGSRVNDSVL VKASDIMHDQ GPVNTEDWLT KKVEDIKPED
860 870 880 890 900
KFFYQYFTTK KTADGKGKKS NKASNFDSDD EMDENEIWSA LVKSRPDVED
910 920 930 940 950
DSDDSELDFA EDDFSDSTSD DEPKLDAIDD EDAKSEGSQE SDQEEGLDED
960 970 980 990 1000
IFYSFDGEQD NSDKKRSFAE SSEEDESSEE EKEEEENKEV SAKRAKKKQR
1010 1020
KNMLKSLPVF ASADDYAQYL DQDSD
Length:1,025
Mass (Da):116,677
Last modified:November 1, 1996 - v1
Checksum:iFB80378727ED71D8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X84162 Genomic DNA. Translation: CAA58976.1.
Z74356 Genomic DNA. Translation: CAA98878.1.
Z49209 Genomic DNA. Translation: CAA89089.1.
BK006938 Genomic DNA. Translation: DAA11906.1.
PIRiS54044.
RefSeqiNP_010345.3. NM_001180368.3.

Genome annotation databases

EnsemblFungiiYDR060W; YDR060W; YDR060W.
GeneIDi851632.
KEGGisce:YDR060W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X84162 Genomic DNA. Translation: CAA58976.1.
Z74356 Genomic DNA. Translation: CAA98878.1.
Z49209 Genomic DNA. Translation: CAA89089.1.
BK006938 Genomic DNA. Translation: DAA11906.1.
PIRiS54044.
RefSeqiNP_010345.3. NM_001180368.3.

3D structure databases

ProteinModelPortaliQ12176.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi32115. 184 interactors.
DIPiDIP-4040N.
IntActiQ12176. 103 interactors.
MINTiMINT-563761.

PTM databases

iPTMnetiQ12176.

Proteomic databases

MaxQBiQ12176.
PRIDEiQ12176.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYDR060W; YDR060W; YDR060W.
GeneIDi851632.
KEGGisce:YDR060W.

Organism-specific databases

EuPathDBiFungiDB:YDR060W.
SGDiS000002467. MAK21.

Phylogenomic databases

GeneTreeiENSGT00390000006395.
HOGENOMiHOG000207540.
InParanoidiQ12176.
KOiK14832.
OMAiYKKVAVK.
OrthoDBiEOG092C13JZ.

Enzyme and pathway databases

BioCyciYEAST:G3O-29668-MONOMER.

Miscellaneous databases

PROiQ12176.

Family and domain databases

InterProiIPR016024. ARM-type_fold.
IPR005612. CCAAT-binding_factor.
[Graphical view]
PfamiPF03914. CBF. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 3 hits.
ProtoNetiSearch...

Entry informationi

Entry nameiMAK21_YEAST
AccessioniPrimary (citable) accession number: Q12176
Secondary accession number(s): D6VS46
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: November 1, 1996
Last modified: November 2, 2016
This is version 142 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 15000 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.