Reviewed, UniProtKB/Swiss-Prot Q12176 (MAK21_YEAST)

Names and origin

Protein names

Recommended name:
    Ribosome biogenesis protein MAK21
Alternative name(s):
    Maintenance of killer protein 21
    Nucleolar complex protein 1

Gene names

Name:	MAK21
Synonyms:	NOC1
Ordered Locus Names:	YDR060W
ORF Names:	D4237, YD9609.14

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	1025 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Required for 60S ribosomal subunit synthesis. Ref.4
Subunit structure	Interacts with NOC2. Forms a nucleolar complex with NOC2 that binds to 90S and 66S pre-ribosomes. Ref.4
Subcellular location	Nucleus › nucleolus.
Miscellaneous	Present with 15000 molecules/cell in log phase SD medium. Ref.5
Sequence similarities	Belongs to the CBF/MAK21 family.

Ontologies

Keywords
Biological process	Ribosome biogenesis
Cellular component	Nucleus
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	ribosomal large subunit assembly Ref.3 Inferred from mutant phenotype. Source: SGD
Cellular component	Noc1p-Noc2p complex Ref.4 Inferred from physical interaction. Source: SGD
Molecular function	protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
EBP2	P36049	1	EBI-10944,EBI-6289
NOC2	P39744	3	EBI-10944,EBI-29259

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1025	1025	Ribosome biogenesis protein MAK21		PRO_0000173472
Regions
	Compositional bias	74 – 77	4	Poly-Asp
	Compositional bias	117 – 122	6	Poly-Asp
	Compositional bias	979 – 986	8	Poly-Glu
Amino acid modifications
	Modified residue	62	1	Phosphoserine Ref.7 Ref.9
	Modified residue	73	1	Phosphoserine Ref.7 Ref.9 Ref.8
	Modified residue	80	1	Phosphoserine Ref.7 Ref.9 Ref.8
	Modified residue	708	1	Phosphotyrosine Ref.9
	Modified residue	710	1	Phosphoserine Ref.9 Ref.8 Ref.6
	Modified residue	878	1	Phosphoserine Ref.9
	Modified residue	977	1	Phosphoserine Ref.7
	Modified residue	978	1	Phosphoserine Ref.7

Sequences

	Sequence		Length	Mass (Da)
	Q12176-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: FB80378727ED71D8	FASTA	1,025	116,677
	10 20 30 40 50 60 MSENNGNPLD LSSLRNKISS KLRDNNSKKA KKTHKGKDVK ASSNSKKVNE DIRREALALG 70 80 90 100 110 120 ASEEDLKLIQ GLSDDDDAKS EQEFDAVADE DADDKGFKND LQNFMKNVGF DQHKLEDVDD 130 140 150 160 170 180 DDIEEESTSS KESKIPAQEK EHAQSNIASS TIEKTSQESI DNGSEQEENT VEEANLSSDQ 190 200 210 220 230 240 EPESESAEKE KKEEKDGGLI TQTTIISSDK LIIPYDKPWY EIPLDPQVGQ NDDVEELSKE 250 260 270 280 290 300 QIEKLFERGK QTLEADNQTY YEEFTKDSSQ AKFMSQILSD GTLNDKISAV TLLIQDSPLH 310 320 330 340 350 360 NTKSLETLVS YCGKKSRNSA LQSLNALKDL FLNGLLPNRK LRYFKNQPGL SMMLNKKTLA 370 380 390 400 410 420 IFYFEDYLKK LFFRVLEVLE VLSHDPIIHV RLQILNHVFD LLTNQPEQEF NLLRLGVNKI 430 440 450 460 470 480 GDIDSKVSSK ASYLLLKLEQ AHPNMKSIVI DAIVDIALRP NADYHTTYYS VITLNQTILK 490 500 510 520 530 540 RSEDSVANKL VKTYFTLFEK FLINTDKDNT NGVVKSNSKS YEEKRKKNFK KGKHGGKSVK 550 560 570 580 590 600 IEKTENEVLD EKNSKLFSAL LTGINRAFPF AQIPASVYEV HMETLFKITH SSNFNTSIQA 610 620 630 640 650 660 LVLINQVTVK AKLNSDRYYR TLYESLFDPR LVNSSKQGIY LNLLYKSLKQ DALNVERVEA 670 680 690 700 710 720 FVKRILQVCS HWLNVGTITG FFFLLIQLAK TVPQIKNLLT NTPVDYEYES DAEEEQGDKD 730 740 750 760 770 780 IKRKEYDGRK RDPKFANAEK SSLWEINNFI NHFHPTVKTY ANAYVTGETE QIAKPDLGLF 790 800 810 820 830 840 TLSHFLDRFV YRSAKQTNTA RGTSIMQPLF SGSRVNDSVL VKASDIMHDQ GPVNTEDWLT 850 860 870 880 890 900 KKVEDIKPED KFFYQYFTTK KTADGKGKKS NKASNFDSDD EMDENEIWSA LVKSRPDVED 910 920 930 940 950 960 DSDDSELDFA EDDFSDSTSD DEPKLDAIDD EDAKSEGSQE SDQEEGLDED IFYSFDGEQD 970 980 990 1000 1010 1020 NSDKKRSFAE SSEEDESSEE EKEEEENKEV SAKRAKKKQR KNMLKSLPVF ASADDYAQYL DQDSD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nucleotide sequence analysis of a 32,500 bp region of the right arm of Saccharomyces cerevisiae chromosome IV." Brandt P., Ramlow S., Otto B., Bloecker H. Yeast 12:85-90(1996) [PubMed: 8789263] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome IV." Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T. , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P. Nature 387:75-78(1997) [PubMed: 9169867] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	"Mak21p of Saccharomyces cerevisiae, a homolog of human CAATT-binding protein, is essential for 60 S ribosomal subunit biogenesis." Edskes H.K., Ohtake Y., Wickner R.B. J. Biol. Chem. 273:28912-28920(1998) [PubMed: 9786894] [Abstract] Cited for: CHARACTERIZATION.
[4]	"Maturation and intranuclear transport of pre-ribosomes requires Noc proteins." Milkereit P., Gadal O., Podtelejnikov A., Trumtel S., Gas N., Petfalski E., Tollervey D., Mann M., Hurt E., Tschochner H. Cell 105:499-509(2001) [PubMed: 11371346] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH NOC2.
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]	"Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway." Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N. Mol. Cell. Proteomics 4:310-327(2005) [PubMed: 15665377] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-710, MASS SPECTROMETRY.
[7]	"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed: 17330950] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-62; SER-73; SER-80; SER-977 AND SER-978, MASS SPECTROMETRY.
[8]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed: 17563356] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-73; SER-80 AND SER-710, MASS SPECTROMETRY.
[9]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-62; SER-73; SER-80; TYR-708; SER-710 AND SER-878, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X84162 Genomic DNA. Translation: CAA58976.1. Z74356 Genomic DNA. Translation: CAA98878.1. Z49209 Genomic DNA. Translation: CAA89089.1.
PIR	S54044.
RefSeq	NP_010345.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:4040N.
IntAct	Q12176. 83 interactions.
STRING	Q12176.
Proteomic databases
PeptideAtlas	Q12176.
PRIDE	Q12176.
Genome annotation databases
Ensembl	YDR060W; YDR060W; YDR060W; Saccharomyces cerevisiae. [Genome view]
GeneID	851632.
KEGG	sce:YDR060W.
NMPDR	fig|4932.3.peg.1090.
Organism-specific databases
CYGD	YDR060w.
SGD	S000002467. MAK21.
Phylogenomic databases
HOGENOM	Q12176.
OMA	KFMSQIL
OrthoDB	EOG9CC5HX
Gene expression databases
ArrayExpress	Q12176.
Genevestigator	Q12176.
GermOnline	YDR060W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR005612. CCAAT-binding_factor. [Graphical view]
Pfam	PF03914. CBF. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	969182.

Entry information

Entry name

MAK21_YEAST

Accession

Primary (citable) accession number: Q12176

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	November 1, 1996
Last modified:	November 24, 2009
This is version 81 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)