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Protein

MutS protein homolog 5

Gene

MSH5

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in meiotic recombination. Facilitate crossovers between homologs during meiosis.

Miscellaneous

Two distinct classes of crossovers have been demonstrated in budding yeast. Class I is MSH4/MSH5 dependent and exhibits crossover interference. Class II is MUS81/MMS4 dependent and exhibits no interference.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi643 – 650ATPSequence analysis8

GO - Molecular functioni

GO - Biological processi

  • chiasma assembly Source: GO_Central
  • mismatch repair Source: GO_Central
  • reciprocal meiotic recombination Source: SGD

Keywordsi

Molecular functionDNA-binding
Biological processDNA damage, DNA repair
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-29549-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
MutS protein homolog 5
Gene namesi
Name:MSH5
Ordered Locus Names:YDL154W
ORF Names:D1542
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

EuPathDBiFungiDB:YDL154W
SGDiS000002313 MSH5

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi643 – 644GA → DV: Abolishes function; no effect on interaction with MSH4. 1 Publication2
Mutagenesisi648G → R: Abolishes function; no effect on interaction with MSH4. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001152061 – 901MutS protein homolog 5Add BLAST901

Proteomic databases

PaxDbiQ12175
PRIDEiQ12175

Interactioni

Subunit structurei

Heterooligomer of MSH4 and MSH5.

Binary interactionsi

WithEntry#Exp.IntActNotes
MSH4P409652EBI-11377,EBI-11371

Protein-protein interaction databases

BioGridi31909, 35 interactors
DIPiDIP-1457N
IntActiQ12175, 9 interactors
MINTiQ12175
STRINGi4932.YDL154W

Structurei

3D structure databases

ProteinModelPortaliQ12175
SMRiQ12175
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the DNA mismatch repair MutS family.Curated

Phylogenomic databases

GeneTreeiENSGT00550000074977
HOGENOMiHOG000113662
InParanoidiQ12175
KOiK08741
OMAiSHACHCA
OrthoDBiEOG092C233G

Family and domain databases

InterProiView protein in InterPro
IPR011184 DNA_mismatch_repair_Msh2
IPR000432 DNA_mismatch_repair_MutS_C
IPR007696 DNA_mismatch_repair_MutS_core
IPR036187 DNA_mismatch_repair_MutS_sf
IPR027417 P-loop_NTPase
PfamiView protein in Pfam
PF05192 MutS_III, 1 hit
PF00488 MutS_V, 1 hit
PIRSFiPIRSF005813 MSH2, 1 hit
SMARTiView protein in SMART
SM00534 MUTSac, 1 hit
SM00533 MUTSd, 1 hit
SUPFAMiSSF48334 SSF48334, 1 hit
SSF52540 SSF52540, 1 hit
PROSITEiView protein in PROSITE
PS00486 DNA_MISMATCH_REPAIR_2, 1 hit

Sequencei

Sequence statusi: Complete.

Q12175-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSHEWLISAS ETMRSIGNGE GLRDKGAVVA NNDGEFNEGD TNREEDSSTI
60 70 80 90 100
FSFDFDEEIV MCIDFSGGKL GCSILDYHTK TLKAFDQDYV VNKTTISSHD
110 120 130 140 150
LIDDADMSSN DISLLLGLLI MEANPTVCLV PARLEDWIFD YIKTKCDEIN
160 170 180 190 200
CRLELQPIKR FKKWDLLQSL QLRGHDNQTI LNDILSNSKF TTTVTLGTVG
210 220 230 240 250
CILANHEQLG EYNDSTASSN MVTGRLVQNA FEDVIHGIRY IDIRDRMVLD
260 270 280 290 300
ENTISALHIF PTAHKLGHDK MMRNGFFSVF ELFNQVSSDY ARRILKSWLI
310 320 330 340 350
NPLTNKKRIE TRYSIIRTLL DKQNAIIFSD LSQSIKRCPD AFGFINQLRS
360 370 380 390 400
GKSTLGTWSK VASFLEKGIA IFQLVSSLKL GSDEANILHD IKNKVDISAL
410 420 430 440 450
KECLRKVETV IDFDTSRDTK TLTINTGVDN RLDECRNIYN HLEGILLDVA
460 470 480 490 500
RETQIFLLNT MPQEDCKTTK SLEKLVNAVY IPQLGYLVTI SVLMEPLLDG
510 520 530 540 550
IPNLQWEEIF RSSENIYFKN GRVLELDETY GDIYGAISDF EIEILFSLQE
560 570 580 590 600
QILRRKTQLT AYNILLSELE ILLSFAQVSA ERNYAEPQLV EDECILEIIN
610 620 630 640 650
GRHALYETFL DNYIPNSTMI DGGLFSELSW CEQNKGRIIV VTGANASGKS
660 670 680 690 700
VYLTQNGLIV YLAQIGCFVP AERARIGIAD KILTRIRTQE TVYKTQSSFL
710 720 730 740 750
LDSQQMAKSL SLATEKSLIL IDEYGKGTDI LDGPSLFGSI MLNMSKSEKC
760 770 780 790 800
PRIIACTHFH ELFNENVLTE NIKGIKHYCT DILISQKYNL LETAHVGEDH
810 820 830 840 850
ESEGITFLFK VKEGISKQSF GIYCAKVCGL SRDIVERAEE LSRMINRGDD
860 870 880 890 900
VVQQCGNLTE KEMREFQKNQ EIVKKFLSWD LDLETTTTSE NLRLKLKNFL

R
Length:901
Mass (Da):102,209
Last modified:November 1, 1997 - v1
Checksum:i505E0344391E30E8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L42517 Genomic DNA Translation: AAA67649.1
X97751 Genomic DNA Translation: CAA66337.1
Z74202 Genomic DNA Translation: CAA98728.1
BK006938 Genomic DNA Translation: DAA11706.1
PIRiS67702
RefSeqiNP_010127.1, NM_001180214.1

Genome annotation databases

EnsemblFungiiYDL154W; YDL154W; YDL154W
GeneIDi851401
KEGGisce:YDL154W

Similar proteinsi

Entry informationi

Entry nameiMSH5_YEAST
AccessioniPrimary (citable) accession number: Q12175
Secondary accession number(s): D6VRJ6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: March 28, 2018
This is version 135 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health