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Q12168 (ENG2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified March 19, 2014. Version 91. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Endo-1,3(4)-beta-glucanase 2

Short name=Endo-1,3-beta-glucanase 2
Short name=Endo-1,4-beta-glucanase 2
EC=3.2.1.6
Alternative name(s):
Laminarinase-2
Gene names
Name:ACF2
Synonyms:ENG2, PCA1
Ordered Locus Names:YLR144C
ORF Names:L3180
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length779 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

Endohydrolysis of (1->3)- or (1->4)-linkages in beta-D-glucans when the glucose residue whose reducing group is involved in the linkage to be hydrolyzed is itself substituted at C-3.

Subcellular location

Cytoplasm Ref.4.

Miscellaneous

Present with 1420 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the glycosyl hydrolase 81 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 779779Endo-1,3(4)-beta-glucanase 2
PRO_0000215819

Sequences

Sequence LengthMass (Da)Tools
Q12168 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 8BCC24DF96414FA7

FASTA77988,403
        10         20         30         40         50         60 
MCYSRQAIPP PVPNRPGGTT NRGPPPLPPR ANVQPPVCSS ENSSKPRENR VAGESLRTPS 

        70         80         90        100        110        120 
SSNPLADSQV NSDNIFQSPV LSNLKAPPSV FNKVQHPVPK PNIDDQSVDP LETNKFYTNM 

       130        140        150        160        170        180 
LLDDNTQPIW THPYSIWFSR DPELFGLAAN HTLASQRVFD TTTNPPRFYF NPTNIKSFVF 

       190        200        210        220        230        240 
KAREFVSSND IKLEFRDMKH MSMCLLMSLS SSQFIEFPLV QGMGFVTAIY HDLGFELRSA 

       250        260        270        280        290        300 
VGFRSLERIS VNERYGKYNI QLENNRNWIL YLTSPDYSFP QDFQISLLDS NTIISSHKIN 

       310        320        330        340        350        360 
GLICQLSADS VPSIDMAAGC YPVYCDLSGQ TVDEHFTNYR FNYTVAGYSQ SGTTLMYALP 

       370        380        390        400        410        420 
HHKAAFTPEM QEREIASSLD STVKGLMTGY LTNSFDMQVQ VPQELGFEPV ALSLNKKADY 

       430        440        450        460        470        480 
SQEKLSKIRE AAVQEVQLSD PQQESNIDSM YFSGKILAKY AWILYVTHYI LHDENLTKEL 

       490        500        510        520        530        540 
LSKLTIAMER FISNQQVLPL NYDVSWKGII SSGSSSQDFG NSYYNDHHFH YSYHVITAAI 

       550        560        570        580        590        600 
ISLVDSDLSG VTNNSWLENN RDWVECLIRD YSGVDNDDPY FPQFRSFDWF NGHSWAKGLF 

       610        620        630        640        650        660 
PSGDGKDEES TSEDVNSCYA IKLWGLVTGN SKLTDIANLQ LGIMRNVFQS YFLYESNNTV 

       670        680        690        700        710        720 
QPKEFIGNKV SGILFENKID HATYFGMEPQ YIHMIHAIPI TSASSWVRTP NFVKEEWEEK 

       730        740        750        760        770 
MQPIIDQVND GWKGIIMLNM ALLDPKFSYD FFSQPDFNRN FLDNGQSLTW SLAYSGAFS 

« Hide

References

« Hide 'large scale' references
[1]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XII."
Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H. expand/collapse author list , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D.
Nature 387:87-90(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[3]"36.8 kb of S.cerevisiae chromosome XII including ACE2, CKI1, PDC5, SLS1, PUT1 and tRNA-Asp."
Delius H.
Submitted (JUN-1995) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 680-779.
Strain: ATCC 204508 / S288c.
[4]"Eng1p, an endo-1,3-beta-glucanase localized at the daughter side of the septum, is involved in cell separation in Saccharomyces cerevisiae."
Baladron V., Ufano S., Duenas E., Martin-Cuadrado A.B., del Rey F., Vazquez de Aldana C.R.
Eukaryot. Cell 1:774-786(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION.
[5]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U53879 Genomic DNA. Translation: AAB82378.1.
Z73316 Genomic DNA. Translation: CAA97716.1.
X91258 Genomic DNA. Translation: CAA62664.1.
BK006945 Genomic DNA. Translation: DAA09454.1.
PIRS64993.
RefSeqNP_013245.1. NM_001182031.1.

3D structure databases

ProteinModelPortalQ12168.
SMRQ12168. Positions 73-777.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid31413. 30 interactions.
DIPDIP-2889N.
IntActQ12168. 19 interactions.
MINTMINT-598257.

Protein family/group databases

CAZyGH81. Glycoside Hydrolase Family 81.

Proteomic databases

PaxDbQ12168.
PeptideAtlasQ12168.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYLR144C; YLR144C; YLR144C.
GeneID850836.
KEGGsce:YLR144C.

Organism-specific databases

CYGDYLR144c.
SGDS000004134. ACF2.

Phylogenomic databases

eggNOGCOG5498.
GeneTreeENSGT00530000069141.
HOGENOMHOG000211468.
OMAAGYIING.
OrthoDBEOG7MSMXB.

Enzyme and pathway databases

BioCycYEAST:G3O-32281-MONOMER.

Gene expression databases

GenevestigatorQ12168.

Family and domain databases

InterProIPR005200. Glyco_hydro_81.
[Graphical view]
PfamPF03639. Glyco_hydro_81. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio967111.

Entry information

Entry nameENG2_YEAST
AccessionPrimary (citable) accession number: Q12168
Secondary accession number(s): D6VYD8, Q07268
Entry history
Integrated into UniProtKB/Swiss-Prot: February 7, 2006
Last sequence update: November 1, 1996
Last modified: March 19, 2014
This is version 91 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XII

Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries