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Q12168

- ENG2_YEAST

UniProt

Q12168 - ENG2_YEAST

Protein

Endo-1,3(4)-beta-glucanase 2

Gene

ACF2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 94 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    Endohydrolysis of (1->3)- or (1->4)-linkages in beta-D-glucans when the glucose residue whose reducing group is involved in the linkage to be hydrolyzed is itself substituted at C-3.

    GO - Molecular functioni

    1. glucan endo-1,3-beta-D-glucosidase activity Source: SGD
    2. glucan endo-1,3-beta-glucanase activity, C-3 substituted reducing group Source: UniProtKB-EC
    3. glucan endo-1,4-beta-glucanase activity, C-3 substituted reducing group Source: UniProtKB-EC
    4. protein binding Source: IntAct

    GO - Biological processi

    1. actin cytoskeleton organization Source: SGD
    2. cell wall macromolecule catabolic process Source: InterPro

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Enzyme and pathway databases

    BioCyciYEAST:G3O-32281-MONOMER.

    Protein family/group databases

    CAZyiGH81. Glycoside Hydrolase Family 81.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Endo-1,3(4)-beta-glucanase 2 (EC:3.2.1.6)
    Short name:
    Endo-1,3-beta-glucanase 2
    Short name:
    Endo-1,4-beta-glucanase 2
    Alternative name(s):
    Laminarinase-2
    Gene namesi
    Name:ACF2
    Synonyms:ENG2, PCA1
    Ordered Locus Names:YLR144C
    ORF Names:L3180
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XII

    Organism-specific databases

    CYGDiYLR144c.
    SGDiS000004134. ACF2.

    Subcellular locationi

    Cytoplasm 1 Publication

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell
    2. intracellular Source: SGD

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 779779Endo-1,3(4)-beta-glucanase 2PRO_0000215819Add
    BLAST

    Proteomic databases

    MaxQBiQ12168.
    PaxDbiQ12168.
    PeptideAtlasiQ12168.

    Expressioni

    Gene expression databases

    GenevestigatoriQ12168.

    Interactioni

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    HSE1P387532EBI-32973,EBI-1382
    LSB1P532814EBI-32973,EBI-23329
    LSB3P436037EBI-32973,EBI-22980
    RVS167P397439EBI-32973,EBI-14500
    YSC84P327937EBI-32973,EBI-24460

    Protein-protein interaction databases

    BioGridi31413. 30 interactions.
    DIPiDIP-2889N.
    IntActiQ12168. 19 interactions.
    MINTiMINT-598257.

    Structurei

    3D structure databases

    ProteinModelPortaliQ12168.
    SMRiQ12168. Positions 73-777.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 81 family.Curated

    Phylogenomic databases

    eggNOGiCOG5498.
    GeneTreeiENSGT00530000069141.
    HOGENOMiHOG000211468.
    OMAiHYSYHVI.
    OrthoDBiEOG7MSMXB.

    Family and domain databases

    InterProiIPR005200. Glyco_hydro_81.
    [Graphical view]
    PfamiPF03639. Glyco_hydro_81. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q12168-1 [UniParc]FASTAAdd to Basket

    « Hide

    MCYSRQAIPP PVPNRPGGTT NRGPPPLPPR ANVQPPVCSS ENSSKPRENR    50
    VAGESLRTPS SSNPLADSQV NSDNIFQSPV LSNLKAPPSV FNKVQHPVPK 100
    PNIDDQSVDP LETNKFYTNM LLDDNTQPIW THPYSIWFSR DPELFGLAAN 150
    HTLASQRVFD TTTNPPRFYF NPTNIKSFVF KAREFVSSND IKLEFRDMKH 200
    MSMCLLMSLS SSQFIEFPLV QGMGFVTAIY HDLGFELRSA VGFRSLERIS 250
    VNERYGKYNI QLENNRNWIL YLTSPDYSFP QDFQISLLDS NTIISSHKIN 300
    GLICQLSADS VPSIDMAAGC YPVYCDLSGQ TVDEHFTNYR FNYTVAGYSQ 350
    SGTTLMYALP HHKAAFTPEM QEREIASSLD STVKGLMTGY LTNSFDMQVQ 400
    VPQELGFEPV ALSLNKKADY SQEKLSKIRE AAVQEVQLSD PQQESNIDSM 450
    YFSGKILAKY AWILYVTHYI LHDENLTKEL LSKLTIAMER FISNQQVLPL 500
    NYDVSWKGII SSGSSSQDFG NSYYNDHHFH YSYHVITAAI ISLVDSDLSG 550
    VTNNSWLENN RDWVECLIRD YSGVDNDDPY FPQFRSFDWF NGHSWAKGLF 600
    PSGDGKDEES TSEDVNSCYA IKLWGLVTGN SKLTDIANLQ LGIMRNVFQS 650
    YFLYESNNTV QPKEFIGNKV SGILFENKID HATYFGMEPQ YIHMIHAIPI 700
    TSASSWVRTP NFVKEEWEEK MQPIIDQVND GWKGIIMLNM ALLDPKFSYD 750
    FFSQPDFNRN FLDNGQSLTW SLAYSGAFS 779
    Length:779
    Mass (Da):88,403
    Last modified:November 1, 1996 - v1
    Checksum:i8BCC24DF96414FA7
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U53879 Genomic DNA. Translation: AAB82378.1.
    Z73316 Genomic DNA. Translation: CAA97716.1.
    X91258 Genomic DNA. Translation: CAA62664.1.
    BK006945 Genomic DNA. Translation: DAA09454.1.
    PIRiS64993.
    RefSeqiNP_013245.1. NM_001182031.1.

    Genome annotation databases

    EnsemblFungiiYLR144C; YLR144C; YLR144C.
    GeneIDi850836.
    KEGGisce:YLR144C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U53879 Genomic DNA. Translation: AAB82378.1 .
    Z73316 Genomic DNA. Translation: CAA97716.1 .
    X91258 Genomic DNA. Translation: CAA62664.1 .
    BK006945 Genomic DNA. Translation: DAA09454.1 .
    PIRi S64993.
    RefSeqi NP_013245.1. NM_001182031.1.

    3D structure databases

    ProteinModelPortali Q12168.
    SMRi Q12168. Positions 73-777.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 31413. 30 interactions.
    DIPi DIP-2889N.
    IntActi Q12168. 19 interactions.
    MINTi MINT-598257.

    Protein family/group databases

    CAZyi GH81. Glycoside Hydrolase Family 81.

    Proteomic databases

    MaxQBi Q12168.
    PaxDbi Q12168.
    PeptideAtlasi Q12168.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YLR144C ; YLR144C ; YLR144C .
    GeneIDi 850836.
    KEGGi sce:YLR144C.

    Organism-specific databases

    CYGDi YLR144c.
    SGDi S000004134. ACF2.

    Phylogenomic databases

    eggNOGi COG5498.
    GeneTreei ENSGT00530000069141.
    HOGENOMi HOG000211468.
    OMAi HYSYHVI.
    OrthoDBi EOG7MSMXB.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-32281-MONOMER.

    Miscellaneous databases

    NextBioi 967111.

    Gene expression databases

    Genevestigatori Q12168.

    Family and domain databases

    InterProi IPR005200. Glyco_hydro_81.
    [Graphical view ]
    Pfami PF03639. Glyco_hydro_81. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XII."
      Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H.
      , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D.
      Nature 387:87-90(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    3. "36.8 kb of S.cerevisiae chromosome XII including ACE2, CKI1, PDC5, SLS1, PUT1 and tRNA-Asp."
      Delius H.
      Submitted (JUN-1995) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 680-779.
      Strain: ATCC 204508 / S288c.
    4. "Eng1p, an endo-1,3-beta-glucanase localized at the daughter side of the septum, is involved in cell separation in Saccharomyces cerevisiae."
      Baladron V., Ufano S., Duenas E., Martin-Cuadrado A.B., del Rey F., Vazquez de Aldana C.R.
      Eukaryot. Cell 1:774-786(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION.
    5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiENG2_YEAST
    AccessioniPrimary (citable) accession number: Q12168
    Secondary accession number(s): D6VYD8, Q07268
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 7, 2006
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 94 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 1420 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome XII
      Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

    External Data

    Dasty 3