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Protein

Endo-1,3(4)-beta-glucanase 2

Gene

ACF2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Endohydrolysis of (1->3)- or (1->4)-linkages in beta-D-glucans when the glucose residue whose reducing group is involved in the linkage to be hydrolyzed is itself substituted at C-3.

GO - Molecular functioni

  1. glucan endo-1,3-beta-D-glucosidase activity Source: SGD
  2. glucan endo-1,3-beta-glucanase activity, C-3 substituted reducing group Source: UniProtKB-EC
  3. glucan endo-1,4-beta-glucanase activity, C-3 substituted reducing group Source: UniProtKB-EC

GO - Biological processi

  1. actin cytoskeleton organization Source: SGD
  2. cell wall macromolecule catabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Enzyme and pathway databases

BioCyciYEAST:G3O-32281-MONOMER.

Protein family/group databases

CAZyiGH81. Glycoside Hydrolase Family 81.

Names & Taxonomyi

Protein namesi
Recommended name:
Endo-1,3(4)-beta-glucanase 2 (EC:3.2.1.6)
Short name:
Endo-1,3-beta-glucanase 2
Short name:
Endo-1,4-beta-glucanase 2
Alternative name(s):
Laminarinase-2
Gene namesi
Name:ACF2
Synonyms:ENG2, PCA1
Ordered Locus Names:YLR144C
ORF Names:L3180
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XII

Organism-specific databases

CYGDiYLR144c.
SGDiS000004134. ACF2.

Subcellular locationi

Cytoplasm 1 Publication

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
  2. intracellular Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 779779Endo-1,3(4)-beta-glucanase 2PRO_0000215819Add
BLAST

Proteomic databases

MaxQBiQ12168.
PaxDbiQ12168.
PeptideAtlasiQ12168.

Expressioni

Gene expression databases

GenevestigatoriQ12168.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
HSE1P387532EBI-32973,EBI-1382
LSB1P532814EBI-32973,EBI-23329
LSB3P436037EBI-32973,EBI-22980
RVS167P397439EBI-32973,EBI-14500
YSC84P327937EBI-32973,EBI-24460

Protein-protein interaction databases

BioGridi31413. 31 interactions.
DIPiDIP-2889N.
IntActiQ12168. 19 interactions.
MINTiMINT-598257.

Structurei

3D structure databases

ProteinModelPortaliQ12168.
SMRiQ12168. Positions 73-777.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 81 family.Curated

Phylogenomic databases

eggNOGiCOG5498.
GeneTreeiENSGT00530000069141.
HOGENOMiHOG000211468.
InParanoidiQ12168.
OMAiHYSYHVI.
OrthoDBiEOG7MSMXB.

Family and domain databases

InterProiIPR005200. Endo-beta-glucanase.
[Graphical view]
PfamiPF03639. Glyco_hydro_81. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q12168-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MCYSRQAIPP PVPNRPGGTT NRGPPPLPPR ANVQPPVCSS ENSSKPRENR
60 70 80 90 100
VAGESLRTPS SSNPLADSQV NSDNIFQSPV LSNLKAPPSV FNKVQHPVPK
110 120 130 140 150
PNIDDQSVDP LETNKFYTNM LLDDNTQPIW THPYSIWFSR DPELFGLAAN
160 170 180 190 200
HTLASQRVFD TTTNPPRFYF NPTNIKSFVF KAREFVSSND IKLEFRDMKH
210 220 230 240 250
MSMCLLMSLS SSQFIEFPLV QGMGFVTAIY HDLGFELRSA VGFRSLERIS
260 270 280 290 300
VNERYGKYNI QLENNRNWIL YLTSPDYSFP QDFQISLLDS NTIISSHKIN
310 320 330 340 350
GLICQLSADS VPSIDMAAGC YPVYCDLSGQ TVDEHFTNYR FNYTVAGYSQ
360 370 380 390 400
SGTTLMYALP HHKAAFTPEM QEREIASSLD STVKGLMTGY LTNSFDMQVQ
410 420 430 440 450
VPQELGFEPV ALSLNKKADY SQEKLSKIRE AAVQEVQLSD PQQESNIDSM
460 470 480 490 500
YFSGKILAKY AWILYVTHYI LHDENLTKEL LSKLTIAMER FISNQQVLPL
510 520 530 540 550
NYDVSWKGII SSGSSSQDFG NSYYNDHHFH YSYHVITAAI ISLVDSDLSG
560 570 580 590 600
VTNNSWLENN RDWVECLIRD YSGVDNDDPY FPQFRSFDWF NGHSWAKGLF
610 620 630 640 650
PSGDGKDEES TSEDVNSCYA IKLWGLVTGN SKLTDIANLQ LGIMRNVFQS
660 670 680 690 700
YFLYESNNTV QPKEFIGNKV SGILFENKID HATYFGMEPQ YIHMIHAIPI
710 720 730 740 750
TSASSWVRTP NFVKEEWEEK MQPIIDQVND GWKGIIMLNM ALLDPKFSYD
760 770
FFSQPDFNRN FLDNGQSLTW SLAYSGAFS
Length:779
Mass (Da):88,403
Last modified:November 1, 1996 - v1
Checksum:i8BCC24DF96414FA7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U53879 Genomic DNA. Translation: AAB82378.1.
Z73316 Genomic DNA. Translation: CAA97716.1.
X91258 Genomic DNA. Translation: CAA62664.1.
BK006945 Genomic DNA. Translation: DAA09454.1.
PIRiS64993.
RefSeqiNP_013245.1. NM_001182031.1.

Genome annotation databases

EnsemblFungiiYLR144C; YLR144C; YLR144C.
GeneIDi850836.
KEGGisce:YLR144C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U53879 Genomic DNA. Translation: AAB82378.1.
Z73316 Genomic DNA. Translation: CAA97716.1.
X91258 Genomic DNA. Translation: CAA62664.1.
BK006945 Genomic DNA. Translation: DAA09454.1.
PIRiS64993.
RefSeqiNP_013245.1. NM_001182031.1.

3D structure databases

ProteinModelPortaliQ12168.
SMRiQ12168. Positions 73-777.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi31413. 31 interactions.
DIPiDIP-2889N.
IntActiQ12168. 19 interactions.
MINTiMINT-598257.

Protein family/group databases

CAZyiGH81. Glycoside Hydrolase Family 81.

Proteomic databases

MaxQBiQ12168.
PaxDbiQ12168.
PeptideAtlasiQ12168.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYLR144C; YLR144C; YLR144C.
GeneIDi850836.
KEGGisce:YLR144C.

Organism-specific databases

CYGDiYLR144c.
SGDiS000004134. ACF2.

Phylogenomic databases

eggNOGiCOG5498.
GeneTreeiENSGT00530000069141.
HOGENOMiHOG000211468.
InParanoidiQ12168.
OMAiHYSYHVI.
OrthoDBiEOG7MSMXB.

Enzyme and pathway databases

BioCyciYEAST:G3O-32281-MONOMER.

Miscellaneous databases

NextBioi967111.

Gene expression databases

GenevestigatoriQ12168.

Family and domain databases

InterProiIPR005200. Endo-beta-glucanase.
[Graphical view]
PfamiPF03639. Glyco_hydro_81. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XII."
    Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H.
    , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D.
    Nature 387:87-90(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. "36.8 kb of S.cerevisiae chromosome XII including ACE2, CKI1, PDC5, SLS1, PUT1 and tRNA-Asp."
    Delius H.
    Submitted (JUN-1995) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 680-779.
    Strain: ATCC 204508 / S288c.
  4. "Eng1p, an endo-1,3-beta-glucanase localized at the daughter side of the septum, is involved in cell separation in Saccharomyces cerevisiae."
    Baladron V., Ufano S., Duenas E., Martin-Cuadrado A.B., del Rey F., Vazquez de Aldana C.R.
    Eukaryot. Cell 1:774-786(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
  5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiENG2_YEAST
AccessioniPrimary (citable) accession number: Q12168
Secondary accession number(s): D6VYD8, Q07268
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 7, 2006
Last sequence update: November 1, 1996
Last modified: January 7, 2015
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1420 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XII
    Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.