Reviewed, UniProtKB/Swiss-Prot Q12168 (ENG2_YEAST)

Names and origin

Protein names

Recommended name:
    Endo-1,3(4)-beta-glucanase 2
      Short name=Endo-1,4-beta-glucanase 2
      Short name=Endo-1,3-beta-glucanase 2
    EC=3.2.1.6
Alternative name(s):
    Laminarinase-2

Gene names

Name:	ACF2
Synonyms:	ENG2, PCA1
Ordered Locus Names:	YLR144C
ORF Names:	L3180

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	779 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	Endohydrolysis of (1->3)- or (1->4)-linkages in beta-D-glucans when the glucose residue whose reducing group is involved in the linkage to be hydrolyzed is itself substituted at C-3.
Subcellular location	Cytoplasm. Ref.3
Miscellaneous	Present with 1420 molecules/cell in log phase SD medium. Ref.4
Sequence similarities	Belongs to the glycosyl hydrolase 81 family.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Glycosidase Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	actin cytoskeleton organization Inferred from direct assay. Source: SGD cell wall macromolecule catabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	endo-1,3(4)-beta-glucanase activity Inferred from electronic annotation. Source: EC glucan endo-1,3-beta-D-glucosidase activity Ref.3 Inferred from direct assay. Source: SGD protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
BOI1	P38041	1	EBI-32973,EBI-3719
EHT1	P38295	1	EBI-32973,EBI-20890
ERG11	P10614	1	EBI-32973,EBI-5127
ERG27	Q12452	1	EBI-32973,EBI-38132
ERG7	P38604	1	EBI-32973,EBI-6572
FAT1	P38225	1	EBI-32973,EBI-6819
GUK1	P15454	1	EBI-32973,EBI-9672
LSB3	P43603	2	EBI-32973,EBI-22980
MYO5	Q04439	1	EBI-32973,EBI-11687
RVS167	P39743	4	EBI-32973,EBI-14500
SEL1	Q04228	1	EBI-32973,EBI-27730
SLC1	P33333	1	EBI-32973,EBI-13494
SNA3	P14359	1	EBI-32973,EBI-26122
SNA4	Q07549	1	EBI-32973,EBI-22078
TGL1	P34163	1	EBI-32973,EBI-19177
TGL4	P36165	1	EBI-32973,EBI-26531
YSC84	P32793	2	EBI-32973,EBI-24460

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 779	779	Endo-1,3(4)-beta-glucanase 2		PRO_0000215819

Sequences

	Sequence		Length	Mass (Da)
	Q12168-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 8BCC24DF96414FA7	FASTA	779	88,403
	10 20 30 40 50 60 MCYSRQAIPP PVPNRPGGTT NRGPPPLPPR ANVQPPVCSS ENSSKPRENR VAGESLRTPS 70 80 90 100 110 120 SSNPLADSQV NSDNIFQSPV LSNLKAPPSV FNKVQHPVPK PNIDDQSVDP LETNKFYTNM 130 140 150 160 170 180 LLDDNTQPIW THPYSIWFSR DPELFGLAAN HTLASQRVFD TTTNPPRFYF NPTNIKSFVF 190 200 210 220 230 240 KAREFVSSND IKLEFRDMKH MSMCLLMSLS SSQFIEFPLV QGMGFVTAIY HDLGFELRSA 250 260 270 280 290 300 VGFRSLERIS VNERYGKYNI QLENNRNWIL YLTSPDYSFP QDFQISLLDS NTIISSHKIN 310 320 330 340 350 360 GLICQLSADS VPSIDMAAGC YPVYCDLSGQ TVDEHFTNYR FNYTVAGYSQ SGTTLMYALP 370 380 390 400 410 420 HHKAAFTPEM QEREIASSLD STVKGLMTGY LTNSFDMQVQ VPQELGFEPV ALSLNKKADY 430 440 450 460 470 480 SQEKLSKIRE AAVQEVQLSD PQQESNIDSM YFSGKILAKY AWILYVTHYI LHDENLTKEL 490 500 510 520 530 540 LSKLTIAMER FISNQQVLPL NYDVSWKGII SSGSSSQDFG NSYYNDHHFH YSYHVITAAI 550 560 570 580 590 600 ISLVDSDLSG VTNNSWLENN RDWVECLIRD YSGVDNDDPY FPQFRSFDWF NGHSWAKGLF 610 620 630 640 650 660 PSGDGKDEES TSEDVNSCYA IKLWGLVTGN SKLTDIANLQ LGIMRNVFQS YFLYESNNTV 670 680 690 700 710 720 QPKEFIGNKV SGILFENKID HATYFGMEPQ YIHMIHAIPI TSASSWVRTP NFVKEEWEEK 730 740 750 760 770 MQPIIDQVND GWKGIIMLNM ALLDPKFSYD FFSQPDFNRN FLDNGQSLTW SLAYSGAFS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XII." Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H. , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D. Nature 387:87-90(1997) [PubMed: 9169871] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	"36.8 kb of S.cerevisiae chromosome XII including ACE2, CKI1, PDC5, SLS1, PUT1 and tRNA-Asp." Delius H. Submitted (JUN-1995) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 680-779. Strain: ATCC 204508 / S288c.
[3]	"Eng1p, an endo-1,3-beta-glucanase localized at the daughter side of the septum, is involved in cell separation in Saccharomyces cerevisiae." Baladron V., Ufano S., Duenas E., Martin-Cuadrado A.B., del Rey F., Vazquez de Aldana C.R. Eukaryot. Cell 1:774-786(2002) [PubMed: 12455695] [Abstract] Cited for: SUBCELLULAR LOCATION.
[4]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U53879 Genomic DNA. Translation: AAB82378.1. Z73316 Genomic DNA. Translation: CAA97716.1. X91258 Genomic DNA. Translation: CAA62664.1.
PIR	S64993.
RefSeq	NP_013245.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:2889N.
IntAct	Q12168. 26 interactions.
STRING	Q12168.
Protein family/group databases
CAZy	GH81. Glycoside Hydrolase Family 81.
Proteomic databases
PeptideAtlas	Q12168.
Genome annotation databases
Ensembl	YLR144C; YLR144C; YLR144C; Saccharomyces cerevisiae. [Genome view]
GeneID	850836.
GenomeReviews	Gene locus YLR144C in contig Y13138_GR.
KEGG	sce:YLR144C.
NMPDR	fig|4932.3.peg.4238.
Organism-specific databases
CYGD	YLR144c.
SGD	S000004134. ACF2.
Phylogenomic databases
HOGENOM	Q12168.
OMA	EFADGRN.
Enzyme and pathway databases
BRENDA	3.2.1.6. 250.
Gene expression databases
ArrayExpress	Q12168.
Genevestigator	Q12168.
GermOnline	YLR144C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR005200. Glyco_hydro_81. [Graphical view]
Pfam	PF03639. Glyco_hydro_81. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	967111.

Entry information

Entry name

ENG2_YEAST

Accession

Primary (citable) accession number: Q12168
Secondary accession number(s): Q07268

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 7, 2006
Last sequence update:	November 1, 1996
Last modified:	November 3, 2009
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)