Q12168 (ENG2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Endo-1,3(4)-beta-glucanase 2
Short name=Endo-1,3-beta-glucanase 2
Short name=Endo-1,4-beta-glucanase 2
EC=3.2.1.6

Alternative name(s):
Laminarinase-2

Gene names

Name:	ACF2
Synonyms:	ENG2, PCA1
Ordered Locus Names:	YLR144C
ORF Names:	L3180

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	779 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	Endohydrolysis of (1->3)- or (1->4)-linkages in beta-D-glucans when the glucose residue whose reducing group is involved in the linkage to be hydrolyzed is itself substituted at C-3.
Subcellular location	Cytoplasm Ref.4.
Miscellaneous	Present with 1420 molecules/cell in log phase SD medium. Ref.5
Sequence similarities	Belongs to the glycosyl hydrolase 81 family.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Glycosidase Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	actin cytoskeleton organization Inferred from direct assay. Source: SGD cell wall macromolecule catabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	endo-1,3(4)-beta-glucanase activity Inferred from electronic annotation. Source: EC glucan endo-1,3-beta-D-glucosidase activity Inferred from direct assay. Source: SGD protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
LSB3	P43603	4	EBI-32973,EBI-22980
RVS167	P39743	6	EBI-32973,EBI-14500
YSC84	P32793	4	EBI-32973,EBI-24460

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 779	779	Endo-1,3(4)-beta-glucanase 2		PRO_0000215819

Sequences

	Sequence		Length	Mass (Da)
	Q12168 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 8BCC24DF96414FA7	FASTA	779	88,403
	10 20 30 40 50 60 MCYSRQAIPP PVPNRPGGTT NRGPPPLPPR ANVQPPVCSS ENSSKPRENR VAGESLRTPS 70 80 90 100 110 120 SSNPLADSQV NSDNIFQSPV LSNLKAPPSV FNKVQHPVPK PNIDDQSVDP LETNKFYTNM 130 140 150 160 170 180 LLDDNTQPIW THPYSIWFSR DPELFGLAAN HTLASQRVFD TTTNPPRFYF NPTNIKSFVF 190 200 210 220 230 240 KAREFVSSND IKLEFRDMKH MSMCLLMSLS SSQFIEFPLV QGMGFVTAIY HDLGFELRSA 250 260 270 280 290 300 VGFRSLERIS VNERYGKYNI QLENNRNWIL YLTSPDYSFP QDFQISLLDS NTIISSHKIN 310 320 330 340 350 360 GLICQLSADS VPSIDMAAGC YPVYCDLSGQ TVDEHFTNYR FNYTVAGYSQ SGTTLMYALP 370 380 390 400 410 420 HHKAAFTPEM QEREIASSLD STVKGLMTGY LTNSFDMQVQ VPQELGFEPV ALSLNKKADY 430 440 450 460 470 480 SQEKLSKIRE AAVQEVQLSD PQQESNIDSM YFSGKILAKY AWILYVTHYI LHDENLTKEL 490 500 510 520 530 540 LSKLTIAMER FISNQQVLPL NYDVSWKGII SSGSSSQDFG NSYYNDHHFH YSYHVITAAI 550 560 570 580 590 600 ISLVDSDLSG VTNNSWLENN RDWVECLIRD YSGVDNDDPY FPQFRSFDWF NGHSWAKGLF 610 620 630 640 650 660 PSGDGKDEES TSEDVNSCYA IKLWGLVTGN SKLTDIANLQ LGIMRNVFQS YFLYESNNTV 670 680 690 700 710 720 QPKEFIGNKV SGILFENKID HATYFGMEPQ YIHMIHAIPI TSASSWVRTP NFVKEEWEEK 730 740 750 760 770 MQPIIDQVND GWKGIIMLNM ALLDPKFSYD FFSQPDFNRN FLDNGQSLTW SLAYSGAFS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XII." Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H. , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D. Nature 387:87-90(1997) [PubMed: 9169871] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"36.8 kb of S.cerevisiae chromosome XII including ACE2, CKI1, PDC5, SLS1, PUT1 and tRNA-Asp." Delius H. Submitted (JUN-1995) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 680-779. Strain: ATCC 204508 / S288c.
[4]	"Eng1p, an endo-1,3-beta-glucanase localized at the daughter side of the septum, is involved in cell separation in Saccharomyces cerevisiae." Baladron V., Ufano S., Duenas E., Martin-Cuadrado A.B., del Rey F., Vazquez de Aldana C.R. Eukaryot. Cell 1:774-786(2002) [PubMed: 12455695] [Abstract] Cited for: SUBCELLULAR LOCATION.
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U53879 Genomic DNA. Translation: AAB82378.1. Z73316 Genomic DNA. Translation: CAA97716.1. X91258 Genomic DNA. Translation: CAA62664.1. BK006945 Genomic DNA. Translation: DAA09454.1.
PIR	S64993.
RefSeq	NP_013245.1. NM_001182031.1.
3D structure databases
ProteinModelPortal	Q12168.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-2889N.
IntAct	Q12168. 17 interactions.
MINT	MINT-598257.
STRING	Q12168.
Protein family/group databases
CAZy	GH81. Glycoside Hydrolase Family 81.
Proteomic databases
PeptideAtlas	Q12168.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YLR144C; YLR144C; YLR144C.
GeneID	850836.
KEGG	sce:YLR144C.
NMPDR	fig|4932.3.peg.4238.
Organism-specific databases
CYGD	YLR144c.
SGD	S000004134. ACF2.
Phylogenomic databases
eggNOG	fuNOG05625.
GeneTree	EFGT00050000000117.
HOGENOM	HBG398584.
OMA	TWHSIKG.
OrthoDB	EOG4DJP4S.
Gene expression databases
ArrayExpress	Q12168.
Genevestigator	Q12168.
GermOnline	YLR144C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR005200. Glyco_hydro_81. [Graphical view]
Pfam	PF03639. Glyco_hydro_81. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	967111.

Entry information

Entry name

ENG2_YEAST

Accession

Primary (citable) accession number: Q12168
Secondary accession number(s): D6VYD8, Q07268

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 7, 2006
Last sequence update:	November 1, 1996
Last modified:	December 14, 2011
This is version 76 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program