Q12167 (RRG1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Required for respiratory growth protein 1, mitochondrial

Gene names

Name:	RRG1
Ordered Locus Names:	YDR065W
ORF Names:	D4258

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	365 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Essential for respiratory growth and required for mitochondrial protein synthesis. Required for vacuolar acidification. Ref.7 Ref.9
Subcellular location	Mitochondrion Ref.5 Ref.8 Ref.10.
Post-translational modification	N-glycosylated. Glycosylation is important for correct localization of the protein. Ref.10
Miscellaneous	Present with 1670 molecules/cell in log phase SD medium. Ref.6
Sequence similarities	Belongs to the RRG1 family.

Ontologies

Keywords
Cellular component	Mitochondrion
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	mitochondrial genome maintenance Inferred from mutant phenotype Ref.9. Source: SGD vacuolar acidification Inferred from mutant phenotype Ref.7. Source: SGD
Cellular component	mitochondrion Inferred from direct assay Ref.5Ref.8. Source: SGD vacuole Inferred from mutant phenotype Ref.7. Source: GOC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 365	365	Required for respiratory growth protein 1, mitochondrial		PRO_0000244438
Experimental info
	Sequence conflict	364	1	G → D in AAS56023. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q12167 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 3DCCF51829BFDBB2	FASTA	365	42,829
	10 20 30 40 50 60 MAQNFGKIPS HKSYVLSLYR TVLRNIPKCC HSYAFQYEIK KTLSIQLFKH KHDKSSWSVY 70 80 90 100 110 120 TLLNEFSLLN NCLLEGKLQE IKNLMKPLKK MKKQLKTTKI LNSLTSLGDV KTNDPEEVRR 130 140 150 160 170 180 FHVLSAYIKR KQDLGLLPAY IPKTYQHKLL LPLALNEHAC LKLFHIQQKL KNGPPSAGLS 190 200 210 220 230 240 YTKEGRNQIW FVRSPINKGR QQSKKLGILI RKERKDSQKN IDNLNFCEIN AAWALHEAIW 250 260 270 280 290 300 EEYLESKKII KVNLPKYLEY AANIPKSTKC NPSSQYQKVK EWVDPVREIM FELHSKSFQR 310 320 330 340 350 360 VEYFNKYKEK LLKNGGQLAY FDKKSKEMYA KRLTLFRKMS KETLPYVTLF IEGRDLPSVL AKYGF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nucleotide sequence analysis of a 32,500 bp region of the right arm of Saccharomyces cerevisiae chromosome IV." Brandt P., Ramlow S., Otto B., Bloecker H. Yeast 12:85-90(1996) [PubMed: 8789263] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome IV." Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T. , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P. Nature 387:75-78(1997) [PubMed: 9169867] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[5]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]	"Characterization of the yeast ionome: a genome-wide analysis of nutrient mineral and trace element homeostasis in Saccharomyces cerevisiae." Eide D.J., Clark S., Nair T.M., Gehl M., Gribskov M., Guerinot M.L., Harper J.F. Genome Biol. 6:R77.1-R77.13(2005) [PubMed: 16168084] [Abstract] Cited for: FUNCTION.
[8]	"Toward the complete yeast mitochondrial proteome: multidimensional separation techniques for mitochondrial proteomics." Reinders J., Zahedi R.P., Pfanner N., Meisinger C., Sickmann A. J. Proteome Res. 5:1543-1554(2006) [PubMed: 16823961] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.
[9]	"Genome-wide deletion mutant analysis reveals genes required for respiratory growth, mitochondrial genome maintenance and mitochondrial protein synthesis in Saccharomyces cerevisiae." Merz S., Westermann B. Genome Biol. 10:R95.1-R95.18(2009) [PubMed: 19751518] [Abstract] Cited for: FUNCTION.
[10]	"Global analysis of the glycoproteome in Saccharomyces cerevisiae reveals new roles for protein glycosylation in eukaryotes." Kung L.A., Tao S.-C., Qian J., Smith M.G., Snyder M., Zhu H. Mol. Syst. Biol. 5:308-308(2009) [PubMed: 19756047] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS], SUBCELLULAR LOCATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X84162 Genomic DNA. Translation: CAA58981.1. Z49209 Genomic DNA. Translation: CAA89094.1. Z74361 Genomic DNA. Translation: CAA98883.1. AY557697 Genomic DNA. Translation: AAS56023.1. BK006938 Genomic DNA. Translation: DAA11911.1.
PIR	S54049.
RefSeq	NP_010350.1. NM_001180373.1.
3D structure databases
ProteinModelPortal	Q12167.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q12167. 1 interaction.
MINT	MINT-2492744.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YDR065W; YDR065W; YDR065W.
GeneID	851637.
KEGG	sce:YDR065W.
NMPDR	fig|4932.3.peg.1095.
Organism-specific databases
CYGD	YDR065w.
SGD	S000002472. RRG1.
Phylogenomic databases
eggNOG	fuNOG10846.
HOGENOM	HBG203139.
OMA	RINANTQ.
OrthoDB	EOG49CTJ6.
Gene expression databases
ArrayExpress	Q12167.
Genevestigator	Q12167.
GermOnline	YDR065W. Saccharomyces cerevisiae.
Family and domain databases
ProtoNet	Search...
Other
NextBio	969197.

Entry information

Entry name

RRG1_YEAST

Accession

Primary (citable) accession number: Q12167
Secondary accession number(s): D6VS51, Q6Q5U4

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 27, 2006
Last sequence update:	November 1, 1996
Last modified:	December 14, 2011
This is version 73 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program