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Q12166

- LEU9_YEAST

UniProt

Q12166 - LEU9_YEAST

Protein

2-isopropylmalate synthase 2, mitochondrial

Gene

LEU9

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 97 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate). Redundant to LEU4, responsible of about 20% of alpha-IPMS activity. Involved in leucine synthesis.1 Publication

    Catalytic activityi

    Acetyl-CoA + 3-methyl-2-oxobutanoate + H2O = (2S)-2-isopropylmalate + CoA.

    Pathwayi

    GO - Molecular functioni

    1. 2-isopropylmalate synthase activity Source: SGD
    2. protein binding Source: IntAct

    GO - Biological processi

    1. leucine biosynthetic process Source: SGD

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    Amino-acid biosynthesis, Branched-chain amino acid biosynthesis, Leucine biosynthesis

    Enzyme and pathway databases

    BioCyciYEAST:MONOMER3O-59.
    UniPathwayiUPA00048; UER00070.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    2-isopropylmalate synthase 2, mitochondrial (EC:2.3.3.13)
    Alternative name(s):
    Alpha-IPM synthase 2
    Alpha-isopropylmalate synthase 2
    Alpha-isopropylmalate synthase II
    Gene namesi
    Name:LEU9
    Ordered Locus Names:YOR108W
    ORF Names:YOR3227w
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XV

    Organism-specific databases

    CYGDiYOR108w.
    SGDiS000005634. LEU9.

    Subcellular locationi

    Mitochondrion 1 Publication

    GO - Cellular componenti

    1. mitochondrion Source: SGD

    Keywords - Cellular componenti

    Mitochondrion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 5050MitochondrionSequence AnalysisAdd
    BLAST
    Chaini51 – 6045542-isopropylmalate synthase 2, mitochondrialPRO_0000255958Add
    BLAST

    Proteomic databases

    MaxQBiQ12166.
    PaxDbiQ12166.
    PeptideAtlasiQ12166.

    Expressioni

    Gene expression databases

    GenevestigatoriQ12166.

    Interactioni

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    LEU4P062083EBI-37359,EBI-10116

    Protein-protein interaction databases

    BioGridi34504. 60 interactions.
    DIPiDIP-4153N.
    IntActiQ12166. 4 interactions.
    MINTiMINT-562265.
    STRINGi4932.YOR108W.

    Structurei

    3D structure databases

    ProteinModelPortaliQ12166.
    SMRiQ12166. Positions 34-592.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiCOG0119.
    GeneTreeiENSGT00390000018527.
    HOGENOMiHOG000110941.
    OMAiREHIIRR.
    OrthoDBiEOG73NGC6.

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    HAMAPiMF_00572. LeuA_type2.
    InterProiIPR013709. 2-isopropylmalate_synth_dimer.
    IPR002034. AIPM/Hcit_synth_CS.
    IPR013785. Aldolase_TIM.
    IPR005668. IPM_Synthase.
    IPR000891. PYR_CT.
    [Graphical view]
    PfamiPF00682. HMGL-like. 1 hit.
    PF08502. LeuA_dimer. 1 hit.
    [Graphical view]
    SMARTiSM00917. LeuA_dimer. 1 hit.
    [Graphical view]
    SUPFAMiSSF110921. SSF110921. 1 hit.
    TIGRFAMsiTIGR00970. leuA_yeast. 1 hit.
    PROSITEiPS00815. AIPM_HOMOCIT_SYNTH_1. 1 hit.
    PS00816. AIPM_HOMOCIT_SYNTH_2. 1 hit.
    PS50991. PYR_CT. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q12166-1 [UniParc]FASTAAdd to Basket

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    MVKHSFIALA EHASKLRRSI PPVKLTYKNM LRDPSVKYRA FAPPKMVKRI    50
    WPDKTIQKAP RWLSTDLRDG NQSLPDPMSV AQKKEYFHKL INIGFKEIEV 100
    SFPSASQTDF DFTRYAVENA PDDVGIQCLV QSREHLIKRT VEALTGAKRA 150
    TIHTYLATSD MFREIVFNMS REEAISKAVE ATKLVRKLTK DDPSQQATRW 200
    SYEFSPECFS DTPGEFAVEI CEAVKKAWEP TEENPIIFNL PATVEVASPN 250
    VYADQIEYFS THITEREKVC ISTHCHNDRG CGVAATELGM LAGADRVEGC 300
    LFGNGERTGN VDLVTVAMNM YTQGVSPNLD FSDLTSISEI VHRCNKIPIP 350
    PRAPYGGELV VSAFSGSHQD AIKKGFAIQN KKQAQGETRW RIPYLPLDPK 400
    DIGRDYEAVI RVNSQSGKGG AAWVIMRSLG LDVPRPMQVD FSNTLQKNAD 450
    ALGRELKSEE ITKLFKETYN YNNNEHIYVT LLNYEVKKLN PERRALVGQV 500
    EINDKVVNIE GYGNGPISSL VDALSNLLNV KLSVQNYSEH SLGSGSATQA 550
    ASFINLSYIK DINNHATSNM WGVGVSEDTG DASIKAVFAT VNNIIHSGDV 600
    LLAE 604
    Length:604
    Mass (Da):67,200
    Last modified:November 1, 1996 - v1
    Checksum:i0E5B049FFBB6097E
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X94335 Genomic DNA. Translation: CAA64028.1.
    Z75016 Genomic DNA. Translation: CAA99306.1.
    BK006948 Genomic DNA. Translation: DAA10883.1.
    PIRiS66993.
    RefSeqiNP_014751.1. NM_001183527.1.

    Genome annotation databases

    EnsemblFungiiYOR108W; YOR108W; YOR108W.
    GeneIDi854275.
    KEGGisce:YOR108W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X94335 Genomic DNA. Translation: CAA64028.1 .
    Z75016 Genomic DNA. Translation: CAA99306.1 .
    BK006948 Genomic DNA. Translation: DAA10883.1 .
    PIRi S66993.
    RefSeqi NP_014751.1. NM_001183527.1.

    3D structure databases

    ProteinModelPortali Q12166.
    SMRi Q12166. Positions 34-592.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 34504. 60 interactions.
    DIPi DIP-4153N.
    IntActi Q12166. 4 interactions.
    MINTi MINT-562265.
    STRINGi 4932.YOR108W.

    Proteomic databases

    MaxQBi Q12166.
    PaxDbi Q12166.
    PeptideAtlasi Q12166.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YOR108W ; YOR108W ; YOR108W .
    GeneIDi 854275.
    KEGGi sce:YOR108W.

    Organism-specific databases

    CYGDi YOR108w.
    SGDi S000005634. LEU9.

    Phylogenomic databases

    eggNOGi COG0119.
    GeneTreei ENSGT00390000018527.
    HOGENOMi HOG000110941.
    OMAi REHIIRR.
    OrthoDBi EOG73NGC6.

    Enzyme and pathway databases

    UniPathwayi UPA00048 ; UER00070 .
    BioCyci YEAST:MONOMER3O-59.

    Miscellaneous databases

    NextBioi 976233.

    Gene expression databases

    Genevestigatori Q12166.

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    HAMAPi MF_00572. LeuA_type2.
    InterProi IPR013709. 2-isopropylmalate_synth_dimer.
    IPR002034. AIPM/Hcit_synth_CS.
    IPR013785. Aldolase_TIM.
    IPR005668. IPM_Synthase.
    IPR000891. PYR_CT.
    [Graphical view ]
    Pfami PF00682. HMGL-like. 1 hit.
    PF08502. LeuA_dimer. 1 hit.
    [Graphical view ]
    SMARTi SM00917. LeuA_dimer. 1 hit.
    [Graphical view ]
    SUPFAMi SSF110921. SSF110921. 1 hit.
    TIGRFAMsi TIGR00970. leuA_yeast. 1 hit.
    PROSITEi PS00815. AIPM_HOMOCIT_SYNTH_1. 1 hit.
    PS00816. AIPM_HOMOCIT_SYNTH_2. 1 hit.
    PS50991. PYR_CT. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XV."
      Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D.
      , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.
      Nature 387:98-102(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. "Identification by functional analysis of the gene encoding alpha-isopropylmalate synthase II (LEU9) in Saccharomyces cerevisiae."
      Casalone E., Barberio C., Cavalieri D., Polsinelli M.
      Yeast 16:539-545(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    5. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiLEU9_YEAST
    AccessioniPrimary (citable) accession number: Q12166
    Secondary accession number(s): D6W2G7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 31, 2006
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 97 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 28800 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome XV
      Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

    External Data

    Dasty 3