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Q12166

- LEU9_YEAST

UniProt

Q12166 - LEU9_YEAST

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Protein
2-isopropylmalate synthase 2, mitochondrial
Gene
LEU9, YOR108W, YOR3227w
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate). Redundant to LEU4, responsible of about 20% of alpha-IPMS activity. Involved in leucine synthesis.1 Publication

Catalytic activityi

Acetyl-CoA + 3-methyl-2-oxobutanoate + H2O = (2S)-2-isopropylmalate + CoA.UniRule annotation

Pathwayi

GO - Molecular functioni

  1. 2-isopropylmalate synthase activity Source: SGD
  2. protein binding Source: IntAct
Complete GO annotation...

GO - Biological processi

  1. leucine biosynthetic process Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Amino-acid biosynthesis, Branched-chain amino acid biosynthesis, Leucine biosynthesis

Enzyme and pathway databases

BioCyciYEAST:MONOMER3O-59.
UniPathwayiUPA00048; UER00070.

Names & Taxonomyi

Protein namesi
Recommended name:
2-isopropylmalate synthase 2, mitochondrial (EC:2.3.3.13)
Alternative name(s):
Alpha-IPM synthase 2
Alpha-isopropylmalate synthase 2
Alpha-isopropylmalate synthase II
Gene namesi
Name:LEU9
Ordered Locus Names:YOR108W
ORF Names:YOR3227w
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XV

Organism-specific databases

CYGDiYOR108w.
SGDiS000005634. LEU9.

Subcellular locationi

Mitochondrion 1 Publication

GO - Cellular componenti

  1. mitochondrion Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 5050Mitochondrion Reviewed prediction
Add
BLAST
Chaini51 – 6045542-isopropylmalate synthase 2, mitochondrialUniRule annotation
PRO_0000255958Add
BLAST

Proteomic databases

MaxQBiQ12166.
PaxDbiQ12166.
PeptideAtlasiQ12166.

Expressioni

Gene expression databases

GenevestigatoriQ12166.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
LEU4P062083EBI-37359,EBI-10116

Protein-protein interaction databases

BioGridi34504. 60 interactions.
DIPiDIP-4153N.
IntActiQ12166. 4 interactions.
MINTiMINT-562265.
STRINGi4932.YOR108W.

Structurei

3D structure databases

ProteinModelPortaliQ12166.
SMRiQ12166. Positions 34-592.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG0119.
GeneTreeiENSGT00390000018527.
HOGENOMiHOG000110941.
OMAiREHIIRR.
OrthoDBiEOG73NGC6.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00572. LeuA_type2.
InterProiIPR013709. 2-isopropylmalate_synth_dimer.
IPR002034. AIPM/Hcit_synth_CS.
IPR013785. Aldolase_TIM.
IPR005668. IPM_Synthase.
IPR000891. PYR_CT.
[Graphical view]
PfamiPF00682. HMGL-like. 1 hit.
PF08502. LeuA_dimer. 1 hit.
[Graphical view]
SMARTiSM00917. LeuA_dimer. 1 hit.
[Graphical view]
SUPFAMiSSF110921. SSF110921. 1 hit.
TIGRFAMsiTIGR00970. leuA_yeast. 1 hit.
PROSITEiPS00815. AIPM_HOMOCIT_SYNTH_1. 1 hit.
PS00816. AIPM_HOMOCIT_SYNTH_2. 1 hit.
PS50991. PYR_CT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q12166-1 [UniParc]FASTAAdd to Basket

« Hide

MVKHSFIALA EHASKLRRSI PPVKLTYKNM LRDPSVKYRA FAPPKMVKRI    50
WPDKTIQKAP RWLSTDLRDG NQSLPDPMSV AQKKEYFHKL INIGFKEIEV 100
SFPSASQTDF DFTRYAVENA PDDVGIQCLV QSREHLIKRT VEALTGAKRA 150
TIHTYLATSD MFREIVFNMS REEAISKAVE ATKLVRKLTK DDPSQQATRW 200
SYEFSPECFS DTPGEFAVEI CEAVKKAWEP TEENPIIFNL PATVEVASPN 250
VYADQIEYFS THITEREKVC ISTHCHNDRG CGVAATELGM LAGADRVEGC 300
LFGNGERTGN VDLVTVAMNM YTQGVSPNLD FSDLTSISEI VHRCNKIPIP 350
PRAPYGGELV VSAFSGSHQD AIKKGFAIQN KKQAQGETRW RIPYLPLDPK 400
DIGRDYEAVI RVNSQSGKGG AAWVIMRSLG LDVPRPMQVD FSNTLQKNAD 450
ALGRELKSEE ITKLFKETYN YNNNEHIYVT LLNYEVKKLN PERRALVGQV 500
EINDKVVNIE GYGNGPISSL VDALSNLLNV KLSVQNYSEH SLGSGSATQA 550
ASFINLSYIK DINNHATSNM WGVGVSEDTG DASIKAVFAT VNNIIHSGDV 600
LLAE 604
Length:604
Mass (Da):67,200
Last modified:November 1, 1996 - v1
Checksum:i0E5B049FFBB6097E
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X94335 Genomic DNA. Translation: CAA64028.1.
Z75016 Genomic DNA. Translation: CAA99306.1.
BK006948 Genomic DNA. Translation: DAA10883.1.
PIRiS66993.
RefSeqiNP_014751.1. NM_001183527.1.

Genome annotation databases

EnsemblFungiiYOR108W; YOR108W; YOR108W.
GeneIDi854275.
KEGGisce:YOR108W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X94335 Genomic DNA. Translation: CAA64028.1 .
Z75016 Genomic DNA. Translation: CAA99306.1 .
BK006948 Genomic DNA. Translation: DAA10883.1 .
PIRi S66993.
RefSeqi NP_014751.1. NM_001183527.1.

3D structure databases

ProteinModelPortali Q12166.
SMRi Q12166. Positions 34-592.
ModBasei Search...

Protein-protein interaction databases

BioGridi 34504. 60 interactions.
DIPi DIP-4153N.
IntActi Q12166. 4 interactions.
MINTi MINT-562265.
STRINGi 4932.YOR108W.

Proteomic databases

MaxQBi Q12166.
PaxDbi Q12166.
PeptideAtlasi Q12166.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YOR108W ; YOR108W ; YOR108W .
GeneIDi 854275.
KEGGi sce:YOR108W.

Organism-specific databases

CYGDi YOR108w.
SGDi S000005634. LEU9.

Phylogenomic databases

eggNOGi COG0119.
GeneTreei ENSGT00390000018527.
HOGENOMi HOG000110941.
OMAi REHIIRR.
OrthoDBi EOG73NGC6.

Enzyme and pathway databases

UniPathwayi UPA00048 ; UER00070 .
BioCyci YEAST:MONOMER3O-59.

Miscellaneous databases

NextBioi 976233.

Gene expression databases

Genevestigatori Q12166.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_00572. LeuA_type2.
InterProi IPR013709. 2-isopropylmalate_synth_dimer.
IPR002034. AIPM/Hcit_synth_CS.
IPR013785. Aldolase_TIM.
IPR005668. IPM_Synthase.
IPR000891. PYR_CT.
[Graphical view ]
Pfami PF00682. HMGL-like. 1 hit.
PF08502. LeuA_dimer. 1 hit.
[Graphical view ]
SMARTi SM00917. LeuA_dimer. 1 hit.
[Graphical view ]
SUPFAMi SSF110921. SSF110921. 1 hit.
TIGRFAMsi TIGR00970. leuA_yeast. 1 hit.
PROSITEi PS00815. AIPM_HOMOCIT_SYNTH_1. 1 hit.
PS00816. AIPM_HOMOCIT_SYNTH_2. 1 hit.
PS50991. PYR_CT. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XV."
    Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D.
    , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.
    Nature 387:98-102(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "Identification by functional analysis of the gene encoding alpha-isopropylmalate synthase II (LEU9) in Saccharomyces cerevisiae."
    Casalone E., Barberio C., Cavalieri D., Polsinelli M.
    Yeast 16:539-545(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  5. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiLEU9_YEAST
AccessioniPrimary (citable) accession number: Q12166
Secondary accession number(s): D6W2G7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 31, 2006
Last sequence update: November 1, 1996
Last modified: June 11, 2014
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 28800 molecules/cell in log phase SD medium.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XV
    Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

External Data

Dasty 3

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