Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q12166 (LEU9_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 94. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
2-isopropylmalate synthase 2, mitochondrial

EC=2.3.3.13
Alternative name(s):
Alpha-IPM synthase 2
Alpha-isopropylmalate synthase 2
Alpha-isopropylmalate synthase II
Gene names
Name:LEU9
Ordered Locus Names:YOR108W
ORF Names:YOR3227w
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length604 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate). Redundant to LEU4, responsible of about 20% of alpha-IPMS activity. Involved in leucine synthesis. Ref.4

Catalytic activity

Acetyl-CoA + 3-methyl-2-oxobutanoate + H2O = (2S)-2-isopropylmalate + CoA. HAMAP-Rule MF_00572

Pathway

Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine from 3-methyl-2-oxobutanoate: step 1/4. HAMAP-Rule MF_00572

Subcellular location

Mitochondrion Ref.5.

Miscellaneous

Present with 28800 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 2 subfamily.

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

LEU4P062083EBI-37359,EBI-10116

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 5050Mitochondrion Potential
Chain51 – 6045542-isopropylmalate synthase 2, mitochondrial HAMAP-Rule MF_00572
PRO_0000255958

Sequences

Sequence LengthMass (Da)Tools
Q12166 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 0E5B049FFBB6097E

FASTA60467,200
        10         20         30         40         50         60 
MVKHSFIALA EHASKLRRSI PPVKLTYKNM LRDPSVKYRA FAPPKMVKRI WPDKTIQKAP 

        70         80         90        100        110        120 
RWLSTDLRDG NQSLPDPMSV AQKKEYFHKL INIGFKEIEV SFPSASQTDF DFTRYAVENA 

       130        140        150        160        170        180 
PDDVGIQCLV QSREHLIKRT VEALTGAKRA TIHTYLATSD MFREIVFNMS REEAISKAVE 

       190        200        210        220        230        240 
ATKLVRKLTK DDPSQQATRW SYEFSPECFS DTPGEFAVEI CEAVKKAWEP TEENPIIFNL 

       250        260        270        280        290        300 
PATVEVASPN VYADQIEYFS THITEREKVC ISTHCHNDRG CGVAATELGM LAGADRVEGC 

       310        320        330        340        350        360 
LFGNGERTGN VDLVTVAMNM YTQGVSPNLD FSDLTSISEI VHRCNKIPIP PRAPYGGELV 

       370        380        390        400        410        420 
VSAFSGSHQD AIKKGFAIQN KKQAQGETRW RIPYLPLDPK DIGRDYEAVI RVNSQSGKGG 

       430        440        450        460        470        480 
AAWVIMRSLG LDVPRPMQVD FSNTLQKNAD ALGRELKSEE ITKLFKETYN YNNNEHIYVT 

       490        500        510        520        530        540 
LLNYEVKKLN PERRALVGQV EINDKVVNIE GYGNGPISSL VDALSNLLNV KLSVQNYSEH 

       550        560        570        580        590        600 
SLGSGSATQA ASFINLSYIK DINNHATSNM WGVGVSEDTG DASIKAVFAT VNNIIHSGDV 


LLAE 

« Hide

References

« Hide 'large scale' references
[1]"DNA sequencing and analysis of 130 kb from yeast chromosome XV."
Voss H., Benes V., Andrade M.A., Valencia A., Rechmann S., Teodoru C., Schwager C., Paces V., Sander C., Ansorge W.
Yeast 13:655-672(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XV."
Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D. expand/collapse author list , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.
Nature 387:98-102(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"Identification by functional analysis of the gene encoding alpha-isopropylmalate synthase II (LEU9) in Saccharomyces cerevisiae."
Casalone E., Barberio C., Cavalieri D., Polsinelli M.
Yeast 16:539-545(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[5]"Global analysis of protein localization in budding yeast."
Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K.
Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[6]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X94335 Genomic DNA. Translation: CAA64028.1.
Z75016 Genomic DNA. Translation: CAA99306.1.
BK006948 Genomic DNA. Translation: DAA10883.1.
PIRS66993.
RefSeqNP_014751.1. NM_001183527.1.

3D structure databases

ProteinModelPortalQ12166.
SMRQ12166. Positions 34-592.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid34504. 60 interactions.
DIPDIP-4153N.
IntActQ12166. 4 interactions.
MINTMINT-562265.
STRING4932.YOR108W.

Proteomic databases

PaxDbQ12166.
PeptideAtlasQ12166.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYOR108W; YOR108W; YOR108W.
GeneID854275.
KEGGsce:YOR108W.

Organism-specific databases

CYGDYOR108w.
SGDS000005634. LEU9.

Phylogenomic databases

eggNOGCOG0119.
GeneTreeENSGT00390000018527.
HOGENOMHOG000110941.
OMAFSTHITE.
OrthoDBEOG73NGC6.

Enzyme and pathway databases

BioCycYEAST:MONOMER3O-59.
UniPathwayUPA00048; UER00070.

Gene expression databases

GenevestigatorQ12166.

Family and domain databases

Gene3D3.20.20.70. 1 hit.
HAMAPMF_00572. LeuA_type2.
InterProIPR013709. 2-isopropylmalate_synth_dimer.
IPR002034. AIPM/Hcit_synth_CS.
IPR013785. Aldolase_TIM.
IPR005668. IPM_Synthase.
IPR000891. PYR_CT.
[Graphical view]
PfamPF00682. HMGL-like. 1 hit.
PF08502. LeuA_dimer. 1 hit.
[Graphical view]
SMARTSM00917. LeuA_dimer. 1 hit.
[Graphical view]
SUPFAMSSF110921. SSF110921. 1 hit.
TIGRFAMsTIGR00970. leuA_yeast. 1 hit.
PROSITEPS00815. AIPM_HOMOCIT_SYNTH_1. 1 hit.
PS00816. AIPM_HOMOCIT_SYNTH_2. 1 hit.
PS50991. PYR_CT. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio976233.

Entry information

Entry nameLEU9_YEAST
AccessionPrimary (citable) accession number: Q12166
Secondary accession number(s): D6W2G7
Entry history
Integrated into UniProtKB/Swiss-Prot: October 31, 2006
Last sequence update: November 1, 1996
Last modified: February 19, 2014
This is version 94 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XV

Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways