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Protein

NAP1-binding protein 2

Gene

NBP2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Negatively regulates the high-osmolarity glycerol (HOG) pathway through its negative regulation of the HOG1 kinase activity. Mediates the binding between the PTC1 phosphatase and the PBS2 MAP/ERK kinase (MEK). With PTC1, regulates endoplasmic reticulum inheritance through the cell wall integrity (CWI) MAPK pathway by modulating the MAPK, SLT2.3 Publications

Miscellaneous

Present with 521 molecules/cell in log phase SD medium.1 Publication

GO - Molecular functioni

  • protein binding, bridging Source: SGD

GO - Biological processi

  • inactivation of MAPK activity Source: SGD
  • negative regulation of MAPK cascade Source: SGD

Enzyme and pathway databases

BioCyciYEAST:G3O-29752-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
NAP1-binding protein 2
Gene namesi
Name:NBP2
Ordered Locus Names:YDR162C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

EuPathDBiFungiDB:YDR162C
SGDiS000002569 NBP2

Subcellular locationi

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002578221 – 236NAP1-binding protein 2Add BLAST236

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei102PhosphoserineCombined sources1
Modified residuei196PhosphoserineCombined sources1
Modified residuei235PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ12163
PaxDbiQ12163
PRIDEiQ12163

PTM databases

iPTMnetiQ12163

Interactioni

Subunit structurei

Interacts with PBS2 and PTC1.1 Publication

Binary interactionsi

Show more details

GO - Molecular functioni

  • protein binding, bridging Source: SGD

Protein-protein interaction databases

BioGridi32213, 577 interactors
DIPiDIP-1538N
IntActiQ12163, 12 interactors
MINTiQ12163
STRINGi4932.YDR162C

Structurei

Secondary structure

1236
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi113 – 117Combined sources5
Beta strandi136 – 144Combined sources9
Beta strandi147 – 151Combined sources5
Beta strandi158 – 162Combined sources5
Helixi163 – 165Combined sources3
Beta strandi166 – 168Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1YN8X-ray1.70A/B/C/D/E/F113-170[»]
2LCSNMR-A110-172[»]
ProteinModelPortaliQ12163
SMRiQ12163
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ12163

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini110 – 171SH3PROSITE-ProRule annotationAdd BLAST62

Keywords - Domaini

SH3 domain

Phylogenomic databases

HOGENOMiHOG000065953
InParanoidiQ12163
OMAiYAYDESN
OrthoDBiEOG092C550C

Family and domain databases

InterProiView protein in InterPro
IPR036028 SH3-like_dom_sf
IPR001452 SH3_domain
PfamiView protein in Pfam
PF00018 SH3_1, 1 hit
SMARTiView protein in SMART
SM00326 SH3, 1 hit
SUPFAMiSSF50044 SSF50044, 1 hit
PROSITEiView protein in PROSITE
PS50002 SH3, 1 hit

Sequencei

Sequence statusi: Complete.

Q12163-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATMETTTQK DTNILKSGLK KTIGVLNEAV LQNGREVEAV QAGNSDTMED
60 70 80 90 100
TETTTIGYIS IKDYAYADSN PLHYGYFDGD NEEDEMVSDS SNGEDTYNKR
110 120 130 140 150
QSITLPDDYI VNQRAVALYD FEPENDNELR LAEGDIVFIS YKHGQGWLVA
160 170 180 190 200
ENESGSKTGL VPEEFVSYIQ PEDGENEVEN KARPFYLTHL ITQSVSPKNN
210 220 230
IDNTNEDEYD DNDEWEDIDD VAEVEADMKT KLDISD
Length:236
Mass (Da):26,571
Last modified:November 1, 1996 - v1
Checksum:iA7458854D5000EA6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D43693 Genomic DNA Translation: BAA07790.1
Z50046 Genomic DNA Translation: CAA90382.1
AY557678 Genomic DNA Translation: AAS56004.1
BK006938 Genomic DNA Translation: DAA12002.1
PIRiS57986
RefSeqiNP_010446.3, NM_001180469.3

Genome annotation databases

EnsemblFungiiYDR162C; YDR162C; YDR162C
GeneIDi851740
KEGGisce:YDR162C

Similar proteinsi

Entry informationi

Entry nameiNBP2_YEAST
AccessioniPrimary (citable) accession number: Q12163
Secondary accession number(s): D6VSE2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 31, 2006
Last sequence update: November 1, 1996
Last modified: May 23, 2018
This is version 135 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

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