Q12163 (NBP2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
NAP1-binding protein 2

Gene names

Name:	NBP2
Ordered Locus Names:	YDR162C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	236 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Negatively regulates the high-osmolarity glycerol (HOG) pathway through its negative regulation of the HOG1 kinase activity. Mediates the binding between the PTC1 phosphatase and the PBS2 MAP/ERK kinase (MEK). With PTC1, regulates endoplasmic reticulum inheritance through the cell wall integrity (CWI) MAPK pathway by modulating the MAPK, SLT2. Ref.5 Ref.7 Ref.8
Subunit structure	Interacts with PBS2 and PTC1. Ref.7
Subcellular location	Cytoplasm Ref.5.
Miscellaneous	Present with 521 molecules/cell in log phase SD medium. Ref.6
Sequence similarities	Contains 1 SH3 domain.

Ontologies

Keywords
Cellular component	Cytoplasm
Domain	SH3 domain
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	hyperosmotic response Inferred from physical interaction Ref.7. Source: SGD negative regulation of protein kinase activity Inferred from direct assay Ref.7. Source: SGD response to heat Inferred from mutant phenotype Ref.5. Source: SGD
Cellular component	cytoplasm Inferred from direct assay Ref.5. Source: SGD nucleus Traceable author statement Ref.7. Source: SGD
Molecular function	protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
PTC1	P35182	4	EBI-34713,EBI-12784
STE20	Q03497	2	EBI-34713,EBI-18285

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 236

236

NAP1-binding protein 2

PRO_0000257822

Regions

Domain

110 – 171

62

SH3

Amino acid modifications

Modified residue

102

1

Phosphoserine Ref.11 Ref.12

Modified residue

104

1

Phosphothreonine Ref.12

Modified residue

196

1

Phosphoserine Ref.9 Ref.10 Ref.12

Modified residue

235

1

Phosphoserine Ref.12

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

236

Helix Strand Turn

Details...

Beta strand

113 – 119

7

Beta strand

136 – 142

7

Beta strand

147 – 151

5

Beta strand

158 – 162

5

Helix

163 – 165

3

Beta strand

166 – 168

3

Sequences

	Sequence		Length	Mass (Da)
	Q12163 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: A7458854D5000EA6	FASTA	236	26,571
	10 20 30 40 50 60 MATMETTTQK DTNILKSGLK KTIGVLNEAV LQNGREVEAV QAGNSDTMED TETTTIGYIS 70 80 90 100 110 120 IKDYAYADSN PLHYGYFDGD NEEDEMVSDS SNGEDTYNKR QSITLPDDYI VNQRAVALYD 130 140 150 160 170 180 FEPENDNELR LAEGDIVFIS YKHGQGWLVA ENESGSKTGL VPEEFVSYIQ PEDGENEVEN 190 200 210 220 230 KARPFYLTHL ITQSVSPKNN IDNTNEDEYD DNDEWEDIDD VAEVEADMKT KLDISD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Okuda A., Fujii-Nakata T., Kikuchi A. Submitted (DEC-1994) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: SFY526.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome IV." Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T. , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P. Nature 387:75-78(1997) [PubMed: 9169867] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[5]	"Yeast Nap1-binding protein Nbp2p is required for mitotic growth at high temperatures and for cell wall integrity." Ohkuni K., Okuda A., Kikuchi A. Genetics 165:517-529(2003) [PubMed: 14573466] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION.
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]	"Nbp2 targets the Ptc1-type 2C Ser/Thr phosphatase to the HOG MAPK pathway." Mapes J., Ota I.M. EMBO J. 23:302-311(2004) [PubMed: 14685261] [Abstract] Cited for: FUNCTION, INTERACTION WITH PTC1 AND PBS2.
[8]	"Ptc1p regulates cortical ER inheritance via Slt2p." Du Y., Walker L., Novick P., Ferro-Novick S. EMBO J. 25:4413-4422(2006) [PubMed: 16977319] [Abstract] Cited for: FUNCTION.
[9]	"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed: 17330950] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-196, MASS SPECTROMETRY. Strain: ADR376.
[10]	"Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry." Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F. Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed: 17287358] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-196, MASS SPECTROMETRY.
[11]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed: 17563356] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-102, MASS SPECTROMETRY.
[12]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-102; THR-104; SER-196 AND SER-235, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

D43693 Genomic DNA. Translation: BAA07790.1.
Z50046 Genomic DNA. Translation: CAA90382.1.
AY557678 Genomic DNA. Translation: AAS56004.1.
BK006938 Genomic DNA. Translation: DAA12002.1.

PIR

S57986.

RefSeq

NP_010446.1. NM_001180469.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1YN8	X-ray	1.70	A/B/C/D/E/F	113-170	[»]

ProteinModelPortal

Q12163.

SMR

Q12163. Positions 113-170.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-1538N.

IntAct

Q12163. 9 interactions.

MINT

MINT-392639.

STRING

Q12163.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblFungi

YDR162C; YDR162C; YDR162C.

GeneID

851740.

KEGG

sce:YDR162C.

NMPDR

fig|4932.3.peg.1195.

Organism-specific databases

CYGD

YDR162c.

SGD

S000002569. NBP2.

Phylogenomic databases

eggNOG

fuNOG12665.

GeneTree

EFGT00050000002185.

HOGENOM

HBG397666.

OMA

ISIKDYA.

OrthoDB

EOG4MSH73.

Gene expression databases

ArrayExpress

Q12163.

Genevestigator

Q12163.

GermOnline

YDR162C. Saccharomyces cerevisiae.

Family and domain databases

InterPro

IPR001452. SH3_domain.
[Graphical view]

Pfam

PF00018. SH3_1. 1 hit.
[Graphical view]

SMART

SM00326. SH3. 1 hit.
[Graphical view]

SUPFAM

SSF50044. SH3. 1 hit.

PROSITE

PS50002. SH3. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

NextBio

969475.

Entry information

Entry name

NBP2_YEAST

Accession

Primary (citable) accession number: Q12163
Secondary accession number(s): D6VSE2

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 31, 2006
Last sequence update:	November 1, 1996
Last modified:	December 14, 2011
This is version 82 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program