Q12159 (YRA1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
RNA annealing protein YRA1

Gene names

Name:	YRA1
Ordered Locus Names:	YDR381W
ORF Names:	D9481.2, D9509.1

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	226 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	RNA-binding RNA annealing protein. May have a role in pre-mRNA metabolism. Component the TREX complex, which operates in coupling transcription elongation to mRNA export. Ref.6
Subunit structure	Component of the transcription/export (TREX) complex, which is at least is formed of SUB2, TEX1 and YRA1 and the THO complex composed of HPR1, MFT1, THO2 and THP1. Interacts with RDS3 and YRA2. Ref.5 Ref.6
Subcellular location	Nucleus.
Sequence similarities	Contains 1 RRM (RNA recognition motif) domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Ligand	RNA-binding
PTM	Phosphoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	mRNA export from nucleus Inferred from genetic interaction Ref.4. Source: SGD regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW transcription-coupled nucleotide-excision repair Inferred from mutant phenotype. Source: SGD
Cellular component	transcription export complex Inferred from physical interaction Ref.4. Source: SGD
Molecular function	RNA binding Inferred from direct assay. Source: SGD nucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 226	226	RNA annealing protein YRA1		PRO_0000082007
Regions
	Domain	78 – 158	81	RRM
	Compositional bias	201 – 210	10	Arg/Lys-rich (basic)
Amino acid modifications
	Modified residue	100	1	Phosphoserine Ref.7

Sequences

	Sequence		Length	Mass (Da)
	Q12159 [UniParc]. Last modified January 1, 1998. Version 2. Checksum: 480B5B6DC0D14BE9	FASTA	226	24,956
	10 20 30 40 50 60 MSANLDKSLD EIIGSNKAGS NRARVGGTRG NGPRRVGKQV GSQRRSLPNR RGPIRKNTRA 70 80 90 100 110 120 PPNAVARVAK LLDTTREVKV NVEGLPRDIK QDAVREFFAS QVGGVQRVLL SYNERGQSTG 130 140 150 160 170 180 MANITFKNGE LARRAVERFN GSPIDGGRSR LRLNLIVDPN QRPVKSLADR IKAMPQKGGN 190 200 210 220 APRPVKRGPN RKAAMAKSQN KPKREKPAKK SLEDLDKEMA DYFEKK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"YRA1, an essential Saccharomyces cerevisiae gene, encodes a novel nuclear protein with RNA annealing activity." Portman D.S., O'Connor J.P., Dreyfuss G. RNA 3:527-537(1997) [PubMed: 9149233] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 39-53 AND 57-70. Strain: BJ5626.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome IV." Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T. , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P. Nature 387:75-78(1997) [PubMed: 9169867] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"TREX is a conserved complex coupling transcription with messenger RNA export." Straesser K., Masuda S., Mason P., Pfannstiel J., Oppizzi M., Rodriguez-Navarro S., Rondon A.G., Aguilera A., Struhl K., Reed R., Hurt E. Nature 417:304-308(2002) [PubMed: 11979277] [Abstract] Cited for: IDENTIFICATION IN TREX COMPLEX, MASS SPECTROMETRY.
[5]	"Rds3p is required for stable U2 snRNP recruitment to the splicing apparatus." Wang Q., Rymond B.C. Mol. Cell. Biol. 23:7339-7349(2003) [PubMed: 14517302] [Abstract] Cited for: INTERACTION WITH RDS3.
[6]	"Biochemical and genetic characterization of Yra1p in budding yeast." Kashyap A.K., Schieltz D., Yates J. III, Kellogg D.R. Yeast 22:43-56(2005) [PubMed: 15584090] [Abstract] Cited for: FUNCTION, INTERACTION WITH YRA2.
[7]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-100, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U72633 Genomic DNA. Translation: AAC09951.1. U28373 Genomic DNA. Translation: AAB64817.1. U32274 Genomic DNA. Translation: AAB64823.1. BK006938 Genomic DNA. Translation: DAA12224.1.
PIR	S61176.
RefSeq	NP_010669.1. NM_001180689.1.
3D structure databases
ProteinModelPortal	Q12159.
SMR	Q12159. Positions 1-156.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-1791N.
IntAct	Q12159. 42 interactions.
MINT	MINT-386145.
STRING	Q12159.
Proteomic databases
PeptideAtlas	Q12159.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YDR381W; YDR381W; YDR381W.
GeneID	851988.
KEGG	sce:YDR381W.
NMPDR	fig|4932.3.peg.1440.
Organism-specific databases
CYGD	YDR381w.
SGD	S000002789. YRA1.
Phylogenomic databases
eggNOG	fuNOG10661.
GeneTree	EFGT00050000001623.
HOGENOM	HBG396717.
OMA	QEMADYF.
OrthoDB	EOG4QNR5V.
Gene expression databases
ArrayExpress	Q12159.
Genevestigator	Q12159.
GermOnline	YDR381W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR012677. Nucleotide-bd_a/b_plait. IPR000504. RRM_dom. [Graphical view]
Gene3D	G3DSA:3.30.70.330. a_b_plait_nuc_bd. 1 hit.
KO	K12881.
Pfam	PF00076. RRM_1. 1 hit. [Graphical view]
SMART	SM00360. RRM. 1 hit. [Graphical view]
PROSITE	PS50102. RRM. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	970148.

Entry information

Entry name

YRA1_YEAST

Accession

Primary (citable) accession number: Q12159
Secondary accession number(s): D6VT14

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	January 1, 1998
Last modified:	December 14, 2011
This is version 106 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program