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Protein

Anaphase-promoting complex subunit 11

Gene

APC11

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Probably catalytic subunit of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin-protein ligase complex that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C is thought to confer substrate specificity and, in the presence of ubiquitin-conjugating E2 enzymes, it catalyzes the formation of protein-ubiquitin conjugates that are subsequently degraded by the 26S proteasome. In early mitosis, the APC/C is activated by CDC20 and targets securin PDS1, the B-type cyclin CLB5, and other anaphase inhibitory proteins for proteolysis, thereby triggering the separation of sister chromatids at the metaphase-to-anaphase transition. In late mitosis and in G1, degradation of CLB5 allows activation of the APC/C by CDH1, which is needed to destroy CDC20 and the B-type cyclin CLB2 to allow exit from mitosis and creating the low CDK state necessary for cytokinesis and for reforming prereplicative complexes in G1 prior to another round of replication. APC11 is required to recruit the ubiquitin-conjugating enzyme E2 to the APC/C.1 Publication

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri52 – 95RING-type; atypicalPROSITE-ProRule annotationAdd BLAST44

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Cell cycle, Cell division, Mitosis, Ubl conjugation pathway

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciYEAST:G3O-29439-MONOMER.
UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
Anaphase-promoting complex subunit 11 (EC:6.3.2.-)
Gene namesi
Name:APC11
Ordered Locus Names:YDL008W
ORF Names:D2900
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

EuPathDBiFungiDB:YDL008W.
SGDiS000002166. APC11.

Subcellular locationi

GO - Cellular componenti

  • anaphase-promoting complex Source: SGD
Complete GO annotation...

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi10S → R in APC11-13; G2/M cell cycle arrest at 37 degrees Celsius. 1 Publication1
Mutagenesisi41C → A: Loss of function. 1 Publication1
Mutagenesisi44C → A: Loss of function. 1 Publication1
Mutagenesisi81W → A: Loss of function. 1 Publication1
Mutagenesisi91C → A: Loss of function. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000557511 – 165Anaphase-promoting complex subunit 11Add BLAST165

Proteomic databases

PRIDEiQ12157.

Interactioni

Subunit structurei

The APC/C is composed of at least 13 subunits that stay tightly associated throughout the cell cycle: APC1, APC2, APC4, APC5, APC9, APC11, CDC16, CDC23, CDC26, CDC27, DOC1, MND2 and SWM1.1 Publication

GO - Molecular functioni

Protein-protein interaction databases

BioGridi32044. 50 interactors.
DIPiDIP-1130N.
IntActiQ12157. 15 interactors.
MINTiMINT-2785255.

Structurei

3D structure databases

ProteinModelPortaliQ12157.
SMRiQ12157.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Contains 1 RING-type zinc finger.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri52 – 95RING-type; atypicalPROSITE-ProRule annotationAdd BLAST44

Keywords - Domaini

Zinc-finger

Phylogenomic databases

GeneTreeiENSGT00550000075186.
HOGENOMiHOG000171951.
InParanoidiQ12157.
KOiK03358.
OMAiGICRASY.
OrthoDBiEOG092C4PUH.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiIPR024991. Apc11.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PfamiPF12861. zf-ANAPC11. 1 hit.
[Graphical view]
PROSITEiPS50089. ZF_RING_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q12157-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKVKINEVHS VFAWSWHIPS TSDEDAANND PIGNDEDEDV CGICRASYNG
60 70 80 90 100
TCPSCKFPGD QCPLVIGLCH HNFHDHCIYR WLDTPTSKGL CPMCRQTFQL
110 120 130 140 150
QKGLAINDAH VQKFVEIVSR RREEMIEEGV AEEFVDFDEP IRQNTDNPIG
160
RQQVDTILDE DFLLR
Length:165
Mass (Da):18,865
Last modified:November 1, 1996 - v1
Checksum:i99F8E8C6BC841934
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z74056 Genomic DNA. Translation: CAA98564.1.
Z48432 Genomic DNA. Translation: CAA88351.1.
BK006938 Genomic DNA. Translation: DAA11839.1.
PIRiS52511.
RefSeqiNP_010276.3. NM_001180067.3.

Genome annotation databases

EnsemblFungiiYDL008W; YDL008W; YDL008W.
GeneIDi851554.
KEGGisce:YDL008W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z74056 Genomic DNA. Translation: CAA98564.1.
Z48432 Genomic DNA. Translation: CAA88351.1.
BK006938 Genomic DNA. Translation: DAA11839.1.
PIRiS52511.
RefSeqiNP_010276.3. NM_001180067.3.

3D structure databases

ProteinModelPortaliQ12157.
SMRiQ12157.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi32044. 50 interactors.
DIPiDIP-1130N.
IntActiQ12157. 15 interactors.
MINTiMINT-2785255.

Proteomic databases

PRIDEiQ12157.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYDL008W; YDL008W; YDL008W.
GeneIDi851554.
KEGGisce:YDL008W.

Organism-specific databases

EuPathDBiFungiDB:YDL008W.
SGDiS000002166. APC11.

Phylogenomic databases

GeneTreeiENSGT00550000075186.
HOGENOMiHOG000171951.
InParanoidiQ12157.
KOiK03358.
OMAiGICRASY.
OrthoDBiEOG092C4PUH.

Enzyme and pathway databases

UniPathwayiUPA00143.
BioCyciYEAST:G3O-29439-MONOMER.

Miscellaneous databases

PROiQ12157.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiIPR024991. Apc11.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PfamiPF12861. zf-ANAPC11. 1 hit.
[Graphical view]
PROSITEiPS50089. ZF_RING_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiAPC11_YEAST
AccessioniPrimary (citable) accession number: Q12157
Secondary accession number(s): D6VRX9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 23, 2004
Last sequence update: November 1, 1996
Last modified: November 2, 2016
This is version 131 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 377 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.