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Q12157

- APC11_YEAST

UniProt

Q12157 - APC11_YEAST

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Protein

Anaphase-promoting complex subunit 11

Gene

APC11

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Probably catalytic subunit of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin-protein ligase complex that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C is thought to confer substrate specificity and, in the presence of ubiquitin-conjugating E2 enzymes, it catalyzes the formation of protein-ubiquitin conjugates that are subsequently degraded by the 26S proteasome. In early mitosis, the APC/C is activated by CDC20 and targets securin PDS1, the B-type cyclin CLB5, and other anaphase inhibitory proteins for proteolysis, thereby triggering the separation of sister chromatids at the metaphase-to-anaphase transition. In late mitosis and in G1, degradation of CLB5 allows activation of the APC/C by CDH1, which is needed to destroy CDC20 and the B-type cyclin CLB2 to allow exit from mitosis and creating the low CDK state necessary for cytokinesis and for reforming prereplicative complexes in G1 prior to another round of replication. APC11 is required to recruit the ubiquitin-conjugating enzyme E2 to the APC/C.1 Publication

Pathwayi

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri52 – 9544RING-type; atypicalPROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. ligase activity Source: UniProtKB-KW
  2. ubiquitin-protein transferase activity Source: SGD
  3. zinc ion binding Source: InterPro

GO - Biological processi

  1. anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process Source: SGD
  2. mitotic nuclear division Source: UniProtKB-KW
  3. protein ubiquitination Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Cell cycle, Cell division, Mitosis, Ubl conjugation pathway

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciYEAST:G3O-29439-MONOMER.
UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
Anaphase-promoting complex subunit 11 (EC:6.3.2.-)
Gene namesi
Name:APC11
Ordered Locus Names:YDL008W
ORF Names:D2900
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome IV

Organism-specific databases

CYGDiYDL008w.
SGDiS000002166. APC11.

Subcellular locationi

GO - Cellular componenti

  1. anaphase-promoting complex Source: SGD
Complete GO annotation...

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi10 – 101S → R in APC11-13; G2/M cell cycle arrest at 37 degrees Celsius. 1 Publication
Mutagenesisi41 – 411C → A: Loss of function. 1 Publication
Mutagenesisi44 – 441C → A: Loss of function. 1 Publication
Mutagenesisi81 – 811W → A: Loss of function. 1 Publication
Mutagenesisi91 – 911C → A: Loss of function. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 165165Anaphase-promoting complex subunit 11PRO_0000055751Add
BLAST

Proteomic databases

PaxDbiQ12157.

Expressioni

Gene expression databases

GenevestigatoriQ12157.

Interactioni

Subunit structurei

The APC/C is composed of at least 13 subunits that stay tightly associated throughout the cell cycle: APC1, APC2, APC4, APC5, APC9, APC11, CDC16, CDC23, CDC26, CDC27, DOC1, MND2 and SWM1.1 Publication

Protein-protein interaction databases

BioGridi32044. 48 interactions.
DIPiDIP-1130N.
IntActiQ12157. 15 interactions.
MINTiMINT-2785255.
STRINGi4932.YDL008W.

Structurei

3D structure databases

ProteinModelPortaliQ12157.
SMRiQ12157. Positions 39-102.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Contains 1 RING-type zinc finger.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri52 – 9544RING-type; atypicalPROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiCOG5194.
GeneTreeiENSGT00550000075186.
HOGENOMiHOG000171951.
InParanoidiQ12157.
KOiK03358.
OMAiGICRASY.
OrthoDBiEOG73Z363.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiIPR024991. Apc11.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
IPR024766. Znf_RING_H2.
[Graphical view]
PfamiPF12861. zf-Apc11. 1 hit.
PF12678. zf-rbx1. 1 hit.
[Graphical view]
SMARTiSM00184. RING. 1 hit.
[Graphical view]
PROSITEiPS50089. ZF_RING_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q12157 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKVKINEVHS VFAWSWHIPS TSDEDAANND PIGNDEDEDV CGICRASYNG
60 70 80 90 100
TCPSCKFPGD QCPLVIGLCH HNFHDHCIYR WLDTPTSKGL CPMCRQTFQL
110 120 130 140 150
QKGLAINDAH VQKFVEIVSR RREEMIEEGV AEEFVDFDEP IRQNTDNPIG
160
RQQVDTILDE DFLLR
Length:165
Mass (Da):18,865
Last modified:November 1, 1996 - v1
Checksum:i99F8E8C6BC841934
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z74056 Genomic DNA. Translation: CAA98564.1.
Z48432 Genomic DNA. Translation: CAA88351.1.
BK006938 Genomic DNA. Translation: DAA11839.1.
PIRiS52511.
RefSeqiNP_010276.3. NM_001180067.3.

Genome annotation databases

EnsemblFungiiYDL008W; YDL008W; YDL008W.
GeneIDi851554.
KEGGisce:YDL008W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z74056 Genomic DNA. Translation: CAA98564.1 .
Z48432 Genomic DNA. Translation: CAA88351.1 .
BK006938 Genomic DNA. Translation: DAA11839.1 .
PIRi S52511.
RefSeqi NP_010276.3. NM_001180067.3.

3D structure databases

ProteinModelPortali Q12157.
SMRi Q12157. Positions 39-102.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 32044. 48 interactions.
DIPi DIP-1130N.
IntActi Q12157. 15 interactions.
MINTi MINT-2785255.
STRINGi 4932.YDL008W.

Proteomic databases

PaxDbi Q12157.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YDL008W ; YDL008W ; YDL008W .
GeneIDi 851554.
KEGGi sce:YDL008W.

Organism-specific databases

CYGDi YDL008w.
SGDi S000002166. APC11.

Phylogenomic databases

eggNOGi COG5194.
GeneTreei ENSGT00550000075186.
HOGENOMi HOG000171951.
InParanoidi Q12157.
KOi K03358.
OMAi GICRASY.
OrthoDBi EOG73Z363.

Enzyme and pathway databases

UniPathwayi UPA00143 .
BioCyci YEAST:G3O-29439-MONOMER.

Miscellaneous databases

NextBioi 968976.

Gene expression databases

Genevestigatori Q12157.

Family and domain databases

Gene3Di 3.30.40.10. 1 hit.
InterProi IPR024991. Apc11.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
IPR024766. Znf_RING_H2.
[Graphical view ]
Pfami PF12861. zf-Apc11. 1 hit.
PF12678. zf-rbx1. 1 hit.
[Graphical view ]
SMARTi SM00184. RING. 1 hit.
[Graphical view ]
PROSITEi PS50089. ZF_RING_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV."
    Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T.
    , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P.
    Nature 387:75-78(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. "Mass spectrometric analysis of the anaphase-promoting complex from yeast: identification of a subunit related to cullins."
    Zachariae W., Shevchenko A., Andrews P.D., Ciosk R., Galova M., Stark M.J., Mann M., Nasmyth K.
    Science 279:1216-1219(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY, SUBUNIT.
  4. "The APC11 RING-H2 finger mediates E2-dependent ubiquitination."
    Leverson J.D., Joazeiro C.A., Page A.M., Huang H., Hieter P., Hunter T.
    Mol. Biol. Cell 11:2315-2325(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, MUTAGENESIS OF SER-10; CYS-41; CYS-44; TRP-81 AND CYS-91.
  5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiAPC11_YEAST
AccessioniPrimary (citable) accession number: Q12157
Secondary accession number(s): D6VRX9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 23, 2004
Last sequence update: November 1, 1996
Last modified: October 29, 2014
This is version 112 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 377 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

External Data

Dasty 3