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Q12157

- APC11_YEAST

UniProt

Q12157 - APC11_YEAST

Protein

Anaphase-promoting complex subunit 11

Gene

APC11

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 111 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Probably catalytic subunit of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin-protein ligase complex that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C is thought to confer substrate specificity and, in the presence of ubiquitin-conjugating E2 enzymes, it catalyzes the formation of protein-ubiquitin conjugates that are subsequently degraded by the 26S proteasome. In early mitosis, the APC/C is activated by CDC20 and targets securin PDS1, the B-type cyclin CLB5, and other anaphase inhibitory proteins for proteolysis, thereby triggering the separation of sister chromatids at the metaphase-to-anaphase transition. In late mitosis and in G1, degradation of CLB5 allows activation of the APC/C by CDH1, which is needed to destroy CDC20 and the B-type cyclin CLB2 to allow exit from mitosis and creating the low CDK state necessary for cytokinesis and for reforming prereplicative complexes in G1 prior to another round of replication. APC11 is required to recruit the ubiquitin-conjugating enzyme E2 to the APC/C.1 Publication

    Pathwayi

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri52 – 9544RING-type; atypicalPROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. ligase activity Source: UniProtKB-KW
    2. ubiquitin-protein transferase activity Source: SGD
    3. zinc ion binding Source: InterPro

    GO - Biological processi

    1. anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process Source: SGD
    2. mitotic nuclear division Source: UniProtKB-KW
    3. protein ubiquitination Source: SGD

    Keywords - Molecular functioni

    Ligase

    Keywords - Biological processi

    Cell cycle, Cell division, Mitosis, Ubl conjugation pathway

    Keywords - Ligandi

    Metal-binding, Zinc

    Enzyme and pathway databases

    BioCyciYEAST:G3O-29439-MONOMER.
    UniPathwayiUPA00143.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Anaphase-promoting complex subunit 11 (EC:6.3.2.-)
    Gene namesi
    Name:APC11
    Ordered Locus Names:YDL008W
    ORF Names:D2900
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome IV

    Organism-specific databases

    CYGDiYDL008w.
    SGDiS000002166. APC11.

    Subcellular locationi

    GO - Cellular componenti

    1. anaphase-promoting complex Source: SGD

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi10 – 101S → R in APC11-13; G2/M cell cycle arrest at 37 degrees Celsius. 1 Publication
    Mutagenesisi41 – 411C → A: Loss of function. 1 Publication
    Mutagenesisi44 – 441C → A: Loss of function. 1 Publication
    Mutagenesisi81 – 811W → A: Loss of function. 1 Publication
    Mutagenesisi91 – 911C → A: Loss of function. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 165165Anaphase-promoting complex subunit 11PRO_0000055751Add
    BLAST

    Proteomic databases

    PaxDbiQ12157.

    Expressioni

    Gene expression databases

    GenevestigatoriQ12157.

    Interactioni

    Subunit structurei

    The APC/C is composed of at least 13 subunits that stay tightly associated throughout the cell cycle: APC1, APC2, APC4, APC5, APC9, APC11, CDC16, CDC23, CDC26, CDC27, DOC1, MND2 and SWM1.1 Publication

    Protein-protein interaction databases

    BioGridi32044. 46 interactions.
    DIPiDIP-1130N.
    IntActiQ12157. 15 interactions.
    MINTiMINT-2785255.
    STRINGi4932.YDL008W.

    Structurei

    3D structure databases

    ProteinModelPortaliQ12157.
    SMRiQ12157. Positions 39-102.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Contains 1 RING-type zinc finger.PROSITE-ProRule annotation

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri52 – 9544RING-type; atypicalPROSITE-ProRule annotationAdd
    BLAST

    Keywords - Domaini

    Zinc-finger

    Phylogenomic databases

    eggNOGiCOG5194.
    GeneTreeiENSGT00550000075186.
    HOGENOMiHOG000171951.
    KOiK03358.
    OMAiGICRASY.
    OrthoDBiEOG73Z363.

    Family and domain databases

    Gene3Di3.30.40.10. 1 hit.
    InterProiIPR024991. Apc11.
    IPR001841. Znf_RING.
    IPR013083. Znf_RING/FYVE/PHD.
    IPR024766. Znf_RING_H2.
    [Graphical view]
    PfamiPF12861. zf-Apc11. 1 hit.
    PF12678. zf-rbx1. 1 hit.
    [Graphical view]
    SMARTiSM00184. RING. 1 hit.
    [Graphical view]
    PROSITEiPS50089. ZF_RING_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q12157-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKVKINEVHS VFAWSWHIPS TSDEDAANND PIGNDEDEDV CGICRASYNG    50
    TCPSCKFPGD QCPLVIGLCH HNFHDHCIYR WLDTPTSKGL CPMCRQTFQL 100
    QKGLAINDAH VQKFVEIVSR RREEMIEEGV AEEFVDFDEP IRQNTDNPIG 150
    RQQVDTILDE DFLLR 165
    Length:165
    Mass (Da):18,865
    Last modified:November 1, 1996 - v1
    Checksum:i99F8E8C6BC841934
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z74056 Genomic DNA. Translation: CAA98564.1.
    Z48432 Genomic DNA. Translation: CAA88351.1.
    BK006938 Genomic DNA. Translation: DAA11839.1.
    PIRiS52511.
    RefSeqiNP_010276.3. NM_001180067.3.

    Genome annotation databases

    EnsemblFungiiYDL008W; YDL008W; YDL008W.
    GeneIDi851554.
    KEGGisce:YDL008W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z74056 Genomic DNA. Translation: CAA98564.1 .
    Z48432 Genomic DNA. Translation: CAA88351.1 .
    BK006938 Genomic DNA. Translation: DAA11839.1 .
    PIRi S52511.
    RefSeqi NP_010276.3. NM_001180067.3.

    3D structure databases

    ProteinModelPortali Q12157.
    SMRi Q12157. Positions 39-102.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 32044. 46 interactions.
    DIPi DIP-1130N.
    IntActi Q12157. 15 interactions.
    MINTi MINT-2785255.
    STRINGi 4932.YDL008W.

    Proteomic databases

    PaxDbi Q12157.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YDL008W ; YDL008W ; YDL008W .
    GeneIDi 851554.
    KEGGi sce:YDL008W.

    Organism-specific databases

    CYGDi YDL008w.
    SGDi S000002166. APC11.

    Phylogenomic databases

    eggNOGi COG5194.
    GeneTreei ENSGT00550000075186.
    HOGENOMi HOG000171951.
    KOi K03358.
    OMAi GICRASY.
    OrthoDBi EOG73Z363.

    Enzyme and pathway databases

    UniPathwayi UPA00143 .
    BioCyci YEAST:G3O-29439-MONOMER.

    Miscellaneous databases

    NextBioi 968976.

    Gene expression databases

    Genevestigatori Q12157.

    Family and domain databases

    Gene3Di 3.30.40.10. 1 hit.
    InterProi IPR024991. Apc11.
    IPR001841. Znf_RING.
    IPR013083. Znf_RING/FYVE/PHD.
    IPR024766. Znf_RING_H2.
    [Graphical view ]
    Pfami PF12861. zf-Apc11. 1 hit.
    PF12678. zf-rbx1. 1 hit.
    [Graphical view ]
    SMARTi SM00184. RING. 1 hit.
    [Graphical view ]
    PROSITEi PS50089. ZF_RING_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV."
      Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T.
      , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P.
      Nature 387:75-78(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    3. "Mass spectrometric analysis of the anaphase-promoting complex from yeast: identification of a subunit related to cullins."
      Zachariae W., Shevchenko A., Andrews P.D., Ciosk R., Galova M., Stark M.J., Mann M., Nasmyth K.
      Science 279:1216-1219(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY, SUBUNIT.
    4. "The APC11 RING-H2 finger mediates E2-dependent ubiquitination."
      Leverson J.D., Joazeiro C.A., Page A.M., Huang H., Hieter P., Hunter T.
      Mol. Biol. Cell 11:2315-2325(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, MUTAGENESIS OF SER-10; CYS-41; CYS-44; TRP-81 AND CYS-91.
    5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiAPC11_YEAST
    AccessioniPrimary (citable) accession number: Q12157
    Secondary accession number(s): D6VRX9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 23, 2004
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 111 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 377 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome IV
      Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

    External Data

    Dasty 3