Reviewed, UniProtKB/Swiss-Prot Q12155 (YL050_YEAST)

Names and origin

Protein names

Recommended name:
    Uncharacterized membrane protein YLR050C

Gene names

Ordered Locus Names:	YLR050C
ORF Names:	L2125

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	161 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Subcellular location	Endoplasmic reticulum membrane; Multi-pass membrane protein.
Miscellaneous	Present with 358 molecules/cell in log phase SD medium. Ref.4

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane
Domain	Transmembrane
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	endoplasmic reticulum Ref.3 Inferred from direct assay. Source: SGD integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
CCW12	Q12127	1	EBI-30729,EBI-5303
ERV25	P54837	1	EBI-30729,EBI-6642
SAC1	P32368	1	EBI-30729,EBI-16210
SPF1	P39986	1	EBI-30729,EBI-3128

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 161	161	Uncharacterized membrane protein YLR050C		PRO_0000247205
Regions
	Topological domain	1 – 10	10	Lumenal Potential
	Transmembrane	11 – 31	21	Potential
	Topological domain	32 – 61	30	Cytoplasmic Potential
	Transmembrane	62 – 82	21	Potential
	Topological domain	83 – 101	19	Lumenal Potential
	Transmembrane	102 – 122	21	Potential
	Topological domain	123 – 161	39	Cytoplasmic Potential

Sequences

	Sequence		Length	Mass (Da)
	Q12155-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 751D352D47FD24ED	FASTA	161	19,089
	10 20 30 40 50 60 MKLGHREQQF YLWYFIVHIP ITIFIDSSVV IPAKWQLGIA QKVVSDHIAK QHDFLLSEKP 70 80 90 100 110 120 EWLYWFVVLE LVLQLPLFVY FVNKFWNSSE LQVNTNSRLK KWLRIYGWNA SLTTLICIVV 130 140 150 160 IFKRGYIPYD VLKTSLSMTQ KCQLASVYLP TFLIPLRLCF V

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XII." Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H. , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D. Nature 387:87-90(1997) [PubMed: 9169871] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[4]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[5]	"A global topology map of the Saccharomyces cerevisiae membrane proteome." Kim H., Melen K., Oesterberg M., von Heijne G. Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed: 16847258] [Abstract] Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X94607 Genomic DNA. Translation: CAA64297.1. Z73222 Genomic DNA. Translation: CAA97580.1. AY557932 Genomic DNA. Translation: AAS56258.1.
PIR	S61624.
RefSeq	NP_013151.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:4746N.
IntAct	Q12155. 8 interactions.
STRING	Q12155.
Proteomic databases
PeptideAtlas	Q12155.
Genome annotation databases
Ensembl	YLR050C; YLR050C; YLR050C; Saccharomyces cerevisiae. [Genome view]
GeneID	850739.
KEGG	sce:YLR050C.
NMPDR	fig|4932.3.peg.4142.
Organism-specific databases
CYGD	YLR050c.
SGD	S000004040. YLR050C.
Phylogenomic databases
HOGENOM	Q12155.
OMA	HIPITIF
OrthoDB	EOG9JQ5F6
Gene expression databases
ArrayExpress	Q12155.
Genevestigator	Q12155.
GermOnline	YLR050C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR016964. TMP_97_prd. [Graphical view]
PIRSF	PIRSF031032. TMP_97_prd. 1 hit.
ProtoNet	Search...
Other Resources
NextBio	966849.

Entry information

Entry name

YL050_YEAST

Accession

Primary (citable) accession number: Q12155

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 25, 2006
Last sequence update:	November 1, 1996
Last modified:	November 24, 2009
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)