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Protein

ATPase GET3

Gene

GET3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

ATPase required for the post-translational delivery of tail-anchored (TA) proteins to the endoplasmic reticulum. Recognizes and selectively binds the transmembrane domain of TA proteins in the cytosol. This complex then targets to the endoplasmic reticulum by membrane-bound receptors GET1 and GET2, where the tail-anchored protein is released for insertion. This process is regulated by ATP binding and hydrolysis. ATP binding drives the homodimer towards the closed dimer state, facilitating recognition of newly synthesized TA membrane proteins. ATP hydrolysis is required for insertion. Subsequently, the homodimer reverts towards the open dimer state, lowering its affinity for the GET1-GET2 receptor, and returning it to the cytosol to initiate a new round of targeting. Cooperates with the HDEL receptor ERD2 to mediate the ATP-dependent retrieval of resident ER proteins that contain a C-terminal H-D-E-L retention signal from the Golgi to the ER. Involved in low-level resistance to the oxyanions arsenite and arsenate, and in heat tolerance.UniRule annotation5 Publications

Miscellaneous

Present with 17300 molecules/cell in log phase SD medium.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei571
Binding sitei245ATP1
Binding sitei272ATP1
Metal bindingi285Zinc; shared with dimeric partnerUniRule annotation1 Publication1
Metal bindingi288Zinc; shared with dimeric partnerUniRule annotation1 Publication1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi26 – 33ATP8
Nucleotide bindingi315 – 322ATP8

GO - Molecular functioni

  • ATPase activity Source: SGD
  • ATP binding Source: UniProtKB-KW
  • guanyl-nucleotide exchange factor activity Source: SGD
  • identical protein binding Source: IntAct
  • metal ion binding Source: UniProtKB-KW
  • unfolded protein binding Source: SGD

GO - Biological processi

  • ATP-independent chaperone mediated protein folding Source: SGD
  • pheromone-dependent signal transduction involved in conjugation with cellular fusion Source: SGD
  • posttranslational protein targeting to endoplasmic reticulum membrane Source: SGD
  • protein insertion into ER membrane Source: SGD
  • response to arsenic-containing substance Source: UniProtKB-KW
  • response to heat Source: SGD
  • response to metal ion Source: SGD
  • retrograde vesicle-mediated transport, Golgi to ER Source: SGD

Keywordsi

Molecular functionHydrolase
Biological processArsenical resistance, ER-Golgi transport, Transport
LigandATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciYEAST:G3O-29503-MONOMER
ReactomeiR-SCE-381038 XBP1(S) activates chaperone genes

Protein family/group databases

TCDBi3.A.21.1.1 the c-terminal tail-anchored membrane protein biogenesis/ insertion complex (tamp-b) family

Names & Taxonomyi

Protein namesi
Recommended name:
ATPase GET3UniRule annotation (EC:3.6.-.-UniRule annotation)
Alternative name(s):
Arsenical pump-driving ATPaseUniRule annotation
Arsenite-stimulated ATPaseUniRule annotation
Golgi to ER traffic protein 3UniRule annotation
Guided entry of tail-anchored proteins 3UniRule annotation
Gene namesi
Name:GET3UniRule annotation
Synonyms:ARR4
Ordered Locus Names:YDL100C
ORF Names:D2371
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

EuPathDBiFungiDB:YDL100C
SGDiS000002258 GET3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Endoplasmic reticulum, Golgi apparatus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi30G → R: Abolishes ATPase activity, leading to secretion of resident ER proteins. 2 Publications1
Mutagenesisi57D → N: Abolishes ATP hydrolysis. 1 Publication1
Mutagenesisi285C → S: Prevents dimerization; when associated with S-288. 1 Publication1
Mutagenesisi288C → S: Prevents dimerization; when associated with S-285. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001522561 – 354ATPase GET3Add BLAST354

Proteomic databases

MaxQBiQ12154
PaxDbiQ12154
PRIDEiQ12154

Interactioni

Subunit structurei

Homodimer. Component of the Golgi to ER traffic (GET) complex, which is composed of GET1, GET2 and GET3. Interacts with the chloride channel protein GEF1.UniRule annotation10 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

  • guanyl-nucleotide exchange factor activity Source: SGD
  • identical protein binding Source: IntAct
  • unfolded protein binding Source: SGD

Protein-protein interaction databases

BioGridi31962, 438 interactors
DIPiDIP-3908N
IntActiQ12154, 58 interactors
MINTiQ12154
STRINGi4932.YDL100C

Structurei

Secondary structure

1354
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi7 – 9Combined sources3
Helixi10 – 13Combined sources4
Beta strandi20 – 26Combined sources7
Beta strandi27 – 30Combined sources4
Helixi31 – 45Combined sources15
Beta strandi47 – 49Combined sources3
Beta strandi51 – 55Combined sources5
Beta strandi57 – 59Combined sources3
Helixi62 – 66Combined sources5
Beta strandi81 – 87Combined sources7
Helixi90 – 98Combined sources9
Turni102 – 104Combined sources3
Beta strandi114 – 116Combined sources3
Helixi117 – 120Combined sources4
Helixi122 – 124Combined sources3
Helixi125 – 130Combined sources6
Helixi136 – 153Combined sources18
Beta strandi155 – 157Combined sources3
Beta strandi161 – 166Combined sources6
Helixi170 – 177Combined sources8
Helixi179 – 188Combined sources10
Helixi191 – 194Combined sources4
Beta strandi195 – 197Combined sources3
Helixi200 – 205Combined sources6
Turni206 – 208Combined sources3
Helixi213 – 230Combined sources18
Turni233 – 235Combined sources3
Beta strandi236 – 245Combined sources10
Helixi246 – 261Combined sources16
Beta strandi268 – 274Combined sources7
Turni277 – 281Combined sources5
Helixi286 – 305Combined sources20
Turni306 – 308Combined sources3
Beta strandi309 – 315Combined sources7
Helixi323 – 335Combined sources13
Helixi340 – 343Combined sources4
Helixi344 – 350Combined sources7

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2WOJX-ray1.99A/B/C/D1-354[»]
3A36X-ray2.80A/B1-354[»]
3A37X-ray3.00A/B1-354[»]
3B2EX-ray3.00A/B/C/D1-354[»]
3H84X-ray2.30A/B1-354[»]
3IDQX-ray3.70A1-354[»]
3SJAX-ray3.00A/B/E/F/I1-354[»]
3SJBX-ray3.30A/B1-354[»]
3SJCX-ray3.20A/B/E/F1-354[»]
3SJDX-ray4.60A/B/C1-354[»]
3VLCX-ray4.50A1-354[»]
3ZS8X-ray3.00A/B1-354[»]
3ZS9X-ray2.10A/B1-354[»]
4PWXX-ray5.40A/B2-354[»]
4XTRX-ray2.05A/B1-354[»]
4XVUX-ray2.35A/B/G/H1-354[»]
4XWOX-ray2.75A/B/G/H/M/N/S/T1-354[»]
5BW8X-ray2.80A/B2-354[»]
5BWKX-ray6.00A/B/C/D/M/N/O/P2-354[»]
ProteinModelPortaliQ12154
SMRiQ12154
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ12154

Family & Domainsi

Sequence similaritiesi

Belongs to the arsA ATPase family.UniRule annotation

Phylogenomic databases

GeneTreeiENSGT00390000003817
HOGENOMiHOG000197637
InParanoidiQ12154
KOiK01551
OMAiDQIADLY
OrthoDBiEOG092C33XF

Family and domain databases

CDDicd02035 ArsA, 1 hit
HAMAPiMF_03112 Asna1_Get3, 1 hit
InterProiView protein in InterPro
IPR025723 Anion-transp_ATPase-like_dom
IPR016300 ATPase_ArsA/GET3
IPR027542 ATPase_ArsA/GET3_euk
IPR027417 P-loop_NTPase
PANTHERiPTHR10803 PTHR10803, 1 hit
PTHR10803:SF3 PTHR10803:SF3, 1 hit
PfamiView protein in Pfam
PF02374 ArsA_ATPase, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
TIGRFAMsiTIGR00345 GET3_arsA_TRC40, 1 hit

Sequencei

Sequence statusi: Complete.

Q12154-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDLTVEPNLH SLITSTTHKW IFVGGKGGVG KTTSSCSIAI QMALSQPNKQ
60 70 80 90 100
FLLISTDPAH NLSDAFGEKF GKDARKVTGM NNLSCMEIDP SAALKDMNDM
110 120 130 140 150
AVSRANNNGS DGQGDDLGSL LQGGALADLT GSIPGIDEAL SFMEVMKHIK
160 170 180 190 200
RQEQGEGETF DTVIFDTAPT GHTLRFLQLP NTLSKLLEKF GEITNKLGPM
210 220 230 240 250
LNSFMGAGNV DISGKLNELK ANVETIRQQF TDPDLTTFVC VCISEFLSLY
260 270 280 290 300
ETERLIQELI SYDMDVNSII VNQLLFAEND QEHNCKRCQA RWKMQKKYLD
310 320 330 340 350
QIDELYEDFH VVKMPLCAGE IRGLNNLTKF SQFLNKEYNP ITDGKVIYEL

EDKE
Length:354
Mass (Da):39,354
Last modified:November 1, 1996 - v1
Checksum:iF795C359B5A4A461
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti43A → T in AAT93183 (PubMed:17322287).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X95644 Genomic DNA Translation: CAA64913.1
Z74148 Genomic DNA Translation: CAA98667.1
AY693164 Genomic DNA Translation: AAT93183.1
BK006938 Genomic DNA Translation: DAA11760.1
PIRiS67642
RefSeqiNP_010183.1, NM_001180159.1

Genome annotation databases

EnsemblFungiiYDL100C; YDL100C; YDL100C
GeneIDi851458
KEGGisce:YDL100C

Similar proteinsi

Entry informationi

Entry nameiGET3_YEAST
AccessioniPrimary (citable) accession number: Q12154
Secondary accession number(s): D6VRQ0, Q6B1B6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: November 1, 1996
Last modified: May 23, 2018
This is version 165 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

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