Reviewed, UniProtKB/Swiss-Prot Q12154 (GET3_YEAST)

Names and origin

Protein names

Recommended name:
    ATPase GET3
    EC=3.6.3.16
Alternative name(s):
    Arsenical pump-driving ATPase
    Arsenite-translocating ATPase
    Arsenical resistance ATPase
    Arsenite-transporting ATPase
    Golgi to ER traffic protein 3

Gene names

Name:	GET3
Synonyms:	ARR4
Ordered Locus Names:	YDL100C
ORF Names:	D2371

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	354 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	ATPase subunit of the GET complex. The GET complex cooperates with the HDEL receptor ERD2 to mediate the ATP-dependent retrieval of resident ER proteins that contain a C-terminal H-D-E-L retention signal from the Golgi to the ER. Involved in low-level resistance to the oxyanions arsenite and arsenate, and in heat tolerance. Ref.5 Ref.8
Catalytic activity	ATP + H2O + arsenite(In) = ADP + phosphate + arsenite(Out).
Subunit structure	Homodimer. Component of the Golgi to ER traffic (GET) complex, which is composed of GET1, GET2 and GET3. Interacts with the chloride channel protein GEF1. Ref.5 Ref.8 Ref.9
Subcellular location	Cytoplasm. Endoplasmic reticulum. Golgi apparatus. Ref.5 Ref.8 Ref.6
Miscellaneous	Present with 17300 molecules/cell in log phase SD medium. Ref.7
Sequence similarities	Belongs to the arsA ATPase family.

Ontologies

Keywords
Biological process	Arsenical resistance ER-Golgi transport Transport
Cellular component	Cytoplasm Endoplasmic reticulum Golgi apparatus
Ligand	ATP-binding Nucleotide-binding
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cellular metal ion homeostasis Inferred from electronic annotation. Source: InterPro pheromone-dependent signal transduction involved in conjugation with cellular fusion Inferred from mutant phenotype. Source: SGD protein insertion into ER membrane Inferred from mutant phenotype. Source: SGD response to arsenic Inferred from electronic annotation. Source: UniProtKB-KW response to heat Ref.5 Inferred from mutant phenotype. Source: SGD response to metal ion Ref.5 Inferred from mutant phenotype. Source: SGD retrograde vesicle-mediated transport, Golgi to ER Ref.8 Inferred from direct assay. Source: SGD
Cellular component	GET complex Ref.8 Inferred from physical interaction. Source: SGD Golgi apparatus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW arsenite transmembrane-transporting ATPase activity Inferred from electronic annotation. Source: EC guanyl-nucleotide exchange factor activity Inferred from direct assay. Source: SGD protein binding Ref.9 Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
	P38868	1	EBI-2989,EBI-24840
	Q04201	1	EBI-2989,EBI-27928
	Q07541	1	EBI-2989,EBI-31593
	Q12125	1	EBI-2989,EBI-36940
CPR2	P23285	1	EBI-2989,EBI-5448
CRH1	P53301	1	EBI-2989,EBI-23440
DBP5	P20449	1	EBI-2989,EBI-5617
GCR2	Q01722	1	EBI-2989,EBI-7469
GEF1	P37020	5	EBI-2989,EBI-7552
GET1	P53192	2	EBI-2989,EBI-23722
INO1	P11986	1	EBI-2989,EBI-9257
MDH2	P22133	1	EBI-2989,EBI-10589
MRP4	P32902	1	EBI-2989,EBI-16248
RNR1	P21524	1	EBI-2989,EBI-15234
RNR2	P09938	1	EBI-2989,EBI-15240
RTN1	Q04947	1	EBI-2989,EBI-38020
SED5	Q01590	2	EBI-2989,EBI-16930
SKG1	P36169	1	EBI-2989,EBI-26550
VMA5	P31412	1	EBI-2989,EBI-20260

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 354	354	ATPase GET3		PRO_0000152256
Regions
	Nucleotide binding	25 – 32	8	ATP Potential
Experimental info
	Mutagenesis	30	1	G → R: Abolishes ATPase activity, leading to secretion of resident ER proteins. Ref.5
	Sequence conflict	43	1	A → T in AAT93183. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Q12154-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: F795C359B5A4A461	FASTA	354	39,354
	10 20 30 40 50 60 MDLTVEPNLH SLITSTTHKW IFVGGKGGVG KTTSSCSIAI QMALSQPNKQ FLLISTDPAH 70 80 90 100 110 120 NLSDAFGEKF GKDARKVTGM NNLSCMEIDP SAALKDMNDM AVSRANNNGS DGQGDDLGSL 130 140 150 160 170 180 LQGGALADLT GSIPGIDEAL SFMEVMKHIK RQEQGEGETF DTVIFDTAPT GHTLRFLQLP 190 200 210 220 230 240 NTLSKLLEKF GEITNKLGPM LNSFMGAGNV DISGKLNELK ANVETIRQQF TDPDLTTFVC 250 260 270 280 290 300 VCISEFLSLY ETERLIQELI SYDMDVNSII VNQLLFAEND QEHNCKRCQA RWKMQKKYLD 310 320 330 340 350 QIDELYEDFH VVKMPLCAGE IRGLNNLTKF SQFLNKEYNP ITDGKVIYEL EDKE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The sequence of a 16,691 bp segment of Saccharomyces cerevisiae chromosome IV identifies the DUN1, PMT1, PMT5, SRP14 and DPR1 genes, and five new open reading frames." Boskovic J., Soler-Mira A., Garcia-Cantalejo J.M., Ballesta J.P.G., Jimenez A., Remacha M.A. Yeast 12:1377-1384(1996) [PubMed: 8923743] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome IV." Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T. , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P. Nature 387:75-78(1997) [PubMed: 9169867] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[3]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	"Systematic identification of protein complexes in Saccharomyces cerevisiae by mass spectrometry." Ho Y., Gruhler A., Heilbut A., Bader G.D., Moore L., Adams S.-L., Millar A., Taylor P., Bennett K., Boutilier K., Yang L., Wolting C., Donaldson I., Schandorff S., Shewnarane J., Vo M., Taggart J., Goudreault M. , Muskat B., Alfarano C., Dewar D., Lin Z., Michalickova K., Willems A.R., Sassi H., Nielsen P.A., Rasmussen K.J., Andersen J.R., Johansen L.E., Hansen L.H., Jespersen H., Podtelejnikov A., Nielsen E., Crawford J., Poulsen V., Soerensen B.D., Matthiesen J., Hendrickson R.C., Gleeson F., Pawson T., Moran M.F., Durocher D., Mann M., Hogue C.W.V., Figeys D., Tyers M. Nature 415:180-183(2002) [PubMed: 11805837] [Abstract] Cited for: IDENTIFICATION IN GET COMPLEX, MASS SPECTROMETRY.
[5]	"The Saccharomyces cerevisiae Arr4p is involved in metal and heat tolerance." Shen J., Hsu C.-M., Kang B.-K., Rosen B.P., Bhattacharjee H. BioMetals 16:369-378(2003) [PubMed: 12680698] [Abstract] Cited for: FUNCTION, MUTAGENESIS OF GLY-30, SUBUNIT, SUBCELLULAR LOCATION.
[6]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[7]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[8]	"Exploration of the function and organization of the yeast early secretory pathway through an epistatic miniarray profile." Schuldiner M., Collins S.R., Thompson N.J., Denic V., Bhamidipati A., Punna T., Ihmels J., Andrews B., Boone C., Greenblatt J.F., Weissman J.S., Krogan N.J. Cell 123:507-519(2005) [PubMed: 16269340] [Abstract] Cited for: FUNCTION, IDENTIFICATION IN GET COMPLEX, SUBCELLULAR LOCATION, INTERACTION WITH GET1.
[9]	"The yeast Arr4p ATPase binds the chloride transporter Gef1p when copper is available in the cytosol." Metz J., Waechter A., Schmidt B., Bujnicki J.M., Schwappach B. J. Biol. Chem. 281:410-417(2006) [PubMed: 16260785] [Abstract] Cited for: INTERACTION WITH GEF1, SUBUNIT.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X95644 Genomic DNA. Translation: CAA64913.1. Z74148 Genomic DNA. Translation: CAA98667.1. AY693164 Genomic DNA. Translation: AAT93183.1.
PIR	S67642.
RefSeq	NP_010183.1.
3D structure databases
HSSP	HSSP built from PDB template 1IHU based on UniProtKB P08690.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:3908N.
IntAct	Q12154. 56 interactions.
Protein family/group databases
TCDB	8.A.26.1.1. golgi to ER trafficking protein (GET3) family.
Proteomic databases
PeptideAtlas	Q12154.
Genome annotation databases
Ensembl	YDL100C. Saccharomyces cerevisiae. [Contig view]
GeneID	851458.
GenomeReviews	Gene locus YDL100C in contig Z71256_GR.
KEGG	sce:YDL100C.
NMPDR	fig|4932.3.peg.921.
Organism-specific databases
CYGD	YDL100c.
SGD	S000002258. GET3.
Yeast-GFP	Search...
Phylogenomic databases
HOGENOM	Q12154.
OMA	Q12154. LSCMEID.
Enzyme and pathway databases
BRENDA	3.6.3.16. 250.
Gene expression databases
ArrayExpress	Q12154.
GermOnline	YDL100C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR003348. ATPase_anion-transp. [Graphical view]
Pfam	PF02374. ArsA_ATPase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00345. arsA. 1 hit.
ProtoNet	Search...
Other Resources
NextBio	968732.

Entry information

Entry name

GET3_YEAST

Accession

Primary (citable) accession number: Q12154
Secondary accession number(s): Q6B1B6

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	November 1, 1996
Last modified:	June 16, 2009
This is version 77 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)