Q12152 (YP150_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Putative serine/threonine-protein kinase YPL150W
EC=2.7.11.1

Gene names

Ordered Locus Names:

YPL150W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	901 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Putative serine/threonine-protein kinase By similarity.
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Induction	By methyl methanesulfonate (MMS). Ref.4
Sequence similarities	Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Contains 1 protein kinase domain.

Ontologies

Keywords
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW protein serine/threonine kinase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 901	901	Putative serine/threonine-protein kinase YPL150W		PRO_0000234368
Regions
	Domain	41 – 287	247	Protein kinase
	Nucleotide binding	47 – 55	9	ATP By similarity
	Compositional bias	398 – 517	120	Ser-rich
	Compositional bias	588 – 712	125	Ser-rich
Sites
	Active site	157	1	Proton acceptor By similarity
	Binding site	70	1	ATP By similarity
Amino acid modifications
	Modified residue	186	1	Phosphothreonine Ref.7
	Modified residue	190	1	Phosphothreonine Ref.7
	Modified residue	371	1	Phosphoserine Ref.6
	Modified residue	374	1	Phosphoserine Ref.6
	Modified residue	456	1	Phosphoserine Ref.6
	Modified residue	530	1	Phosphoserine Ref.7
	Modified residue	533	1	Phosphoserine Ref.5 Ref.7
	Modified residue	614	1	Phosphoserine Ref.7

Sequences

	Sequence		Length	Mass (Da)
	Q12152 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 7E32C5ECB353A06C	FASTA	901	100,646
	10 20 30 40 50 60 MVNPVGSSKL EQNNIKSIIG SSYNRLYSQF TSDELTEVGN YKILKQIGEG SFGKVYLALH 70 80 90 100 110 120 RPTHRKVCLK TSDKNDPNIV REVFYHRQFD FPYITKLYEV IVTESKVWMA LEYCPGKELY 130 140 150 160 170 180 DHLLSLRRIS LLECGELFAQ ISGAVYYAHS MHCVHRDLKL ENILLDKNGN AKLTDFGFTR 190 200 210 220 230 240 ECMTKTTLET VCGTTVYMAP ELIERRTYDG FKIDIWSLGV ILYTLITGYL PFDDDDEAKT 250 260 270 280 290 300 KWKIVNEEPK YDAKVIPDDA RDLISRLLAK NPGERPSLSQ VLRHPFLQPY GSVVLDQTQK 310 320 330 340 350 360 ILCRQRSGGT QFKSKLERRL LKRLKQSGVD TQAIKQSILK KKCDSLSGLW LLLLAQGKKQ 370 380 390 400 410 420 ENCKYPKRSR SVLSVKKVIE SATHNDTNGI SEDVLKPSLE LSRAASLSKM LNKGSDFVTS 430 440 450 460 470 480 MTPVSRKKSK DSAKVLNPTL SKISSQRAYS HSIAGSPRKS NNFLQKVSSF FKSKKSSNSN 490 500 510 520 530 540 SNNSIHTNVS ESLIASNRGA PSSGSFLKKN SGSIQKSRTD TVANPSRTES IGSLNENVAG 550 560 570 580 590 600 AIVPRSANNT TLENKKTSGN EIGLKVAPEL LLNEHIRIEE PRLKRFKSSI SSEISQTSTG 610 620 630 640 650 660 NYDSESAENS RSISFDGKVS PPPIRNRPLS EISQISNDTY ISEYSTDGNN SSFKISDTIK 670 680 690 700 710 720 PSYIRKGSET TSQYSASSEK MTNGYGRKFV RRDLSIVSTA SSTSERSSRT DSFYDITTAT 730 740 750 760 770 780 PVVTTDNRRN KNNNLKESVL PRFGTQRPWT GKRTYTTSRH GKNARRSSKR GLFKITSSNT 790 800 810 820 830 840 DSIIQEVSSS EEEDHNVIYS KGKGLPTPVL QTKGLIENGL NERDEEGDDE YAIHTDGEFS 850 860 870 880 890 900 IKPQFSDDVI DKQNHLPSVK AVATKRSLSE GSNWSSSYLD SDNNRRRVSS LLVEDGGNPT A

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The sequence of 55 kb on the left arm of yeast chromosome XVI identifies a small nuclear RNA, a new putative protein kinase and two new putative regulators." Purnelle B., Coster F., Goffeau A. Yeast 12:1483-1492(1996) [PubMed: 8948103] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI." Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M. , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J. Nature 387:103-105(1997) [PubMed: 9169875] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Global response of Saccharomyces cerevisiae to an alkylating agent." Jelinsky S.A., Samson L.D. Proc. Natl. Acad. Sci. U.S.A. 96:1486-1491(1999) [PubMed: 9990050] [Abstract] Cited for: INDUCTION.
[5]	"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed: 17330950] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-533, MASS SPECTROMETRY. Strain: ADR376.
[6]	"Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry." Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F. Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed: 17287358] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-371; SER-374 AND SER-456, MASS SPECTROMETRY.
[7]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-186; THR-190; SER-530; SER-533 AND SER-614, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X96770 Genomic DNA. Translation: CAA65571.1. Z73506 Genomic DNA. Translation: CAA97855.1. BK006949 Genomic DNA. Translation: DAA11285.1.
PIR	S65161.
RefSeq	NP_015175.1. NM_001183964.1.
3D structure databases
ProteinModelPortal	Q12152.
SMR	Q12152. Positions 29-355.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-3996N.
IntAct	Q12152. 7 interactions.
MINT	MINT-531665.
STRING	Q12152.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YPL150W; YPL150W; YPL150W.
GeneID	855953.
KEGG	sce:YPL150W.
NMPDR	fig|4932.3.peg.6305.
Organism-specific databases
CYGD	YPL150w.
SGD	S000006071. YPL150W.
Phylogenomic databases
eggNOG	fuNOG05569.
GeneTree	EFGT00070000008711.
HOGENOM	HBG203877.
OMA	FYDITTA.
OrthoDB	EOG45MRFM.
Gene expression databases
ArrayExpress	Q12152.
Genevestigator	Q12152.
GermOnline	YPL150W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR017442. Se/Thr_kinase-like_dom. IPR008271. Ser/Thr_kinase_AS. IPR002290. Ser/Thr_kinase_dom. IPR020658. Ser/Thr_kinase_Ppk16. [Graphical view]
PANTHER	PTHR24345:SF42. PTHR24345:SF42. 1 hit.
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
SMART	SM00220. S_TKc. 1 hit. [Graphical view]
SUPFAM	SSF56112. Kinase_like. 1 hit.
PROSITE	PS00107. PROTEIN_KINASE_ATP. False negative. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	980738.

Entry information

Entry name

YP150_YEAST

Accession

Primary (citable) accession number: Q12152
Secondary accession number(s): D6W3L9

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 16, 2006
Last sequence update:	November 1, 1996
Last modified:	December 14, 2011
This is version 97 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program