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Protein

Sterol uptake control protein 2

Gene

UPC2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcription factor that is involved in activation of anaerobic genes such as DAN/TIR cell wall mannoprotein genes and YML083c. Appears to bind to anaerobic response elements (AR1) with the consensus sequence 5'-TCGTTYAG-3' present in the promoter regions of DAN/TIR genes. Involved in sterol uptake and regulation of the sterol biosynthesis. Binds to sterol regulatory elements (SRE) with the consensus sequence 5'-TCGTATA-3' present in ERG2 and ERG3 promoters. May be involved in down-regulation of CWP2 during anaerobic adaptation.5 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi50 – 8031Zn(2)-C6 fungal-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

  • cellular response to hypoxia Source: SGD
  • positive regulation of ergosterol biosynthetic process by positive regulation of transcription from RNA polymerase II promoter Source: SGD
  • positive regulation of filamentous growth of a population of unicellular organisms in response to starvation by positive regulation of transcription from RNA polymerase II promoter Source: SGD
  • positive regulation of sterol import by positive regulation of transcription from RNA polymerase II promoter Source: SGD
  • positive regulation of transcription from RNA polymerase II promoter Source: SGD
  • transcription from RNA polymerase II promoter Source: GOC
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Enzyme and pathway databases

BioCyciYEAST:G3O-29795-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Sterol uptake control protein 2
Alternative name(s):
Mannoprotein regulation by oxygen protein 4
Gene namesi
Name:UPC2
Synonyms:MOX4
Ordered Locus Names:YDR213W
ORF Names:YD8142.14, YD8142B.05
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

EuPathDBiFungiDB:YDR213W.
SGDiS000002621. UPC2.

Subcellular locationi

GO - Cellular componenti

  • membrane Source: SGD
  • nucleus Source: SGD
  • perinuclear region of cytoplasm Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi888 – 8881G → A: Increased aerobic expression of DAN1. 3 Publications
Mutagenesisi888 – 8881G → D in upc2-1; increases aerobic sterol synthesis and uptake. Increases also aerobic expression of DAN1 and sensibility to NaCl and LiCl. 3 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 913913Sterol uptake control protein 2PRO_0000114988Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei122 – 1221PhosphothreonineCombined sources
Modified residuei519 – 5191PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ12151.

PTM databases

iPTMnetiQ12151.

Expressioni

Inductioni

Induced by anaerobic conditions, hypoxia and cold. Repressed by heme.1 Publication

Interactioni

Protein-protein interaction databases

BioGridi32267. 96 interactions.
DIPiDIP-6262N.
MINTiMINT-2786657.

Structurei

Secondary structure

1
913
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi607 – 61812Combined sources
Helixi621 – 6266Combined sources
Helixi632 – 6365Combined sources
Helixi638 – 6458Combined sources
Helixi647 – 66115Combined sources
Helixi670 – 68617Combined sources
Helixi687 – 6926Combined sources
Helixi695 – 71117Combined sources
Helixi732 – 74413Combined sources
Helixi756 – 7594Combined sources
Turni767 – 7693Combined sources
Beta strandi773 – 7764Combined sources
Beta strandi784 – 7874Combined sources
Helixi788 – 7903Combined sources
Beta strandi795 – 7973Combined sources
Helixi800 – 81112Combined sources
Helixi817 – 8248Combined sources
Helixi825 – 8273Combined sources
Helixi831 – 8399Combined sources
Helixi842 – 86120Combined sources
Turni871 – 8733Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4N9NX-ray2.90A/B598-714[»]
A/B726-878[»]
ProteinModelPortaliQ12151.
SMRiQ12151. Positions 604-878.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili303 – 34644Sequence analysisAdd
BLAST
Coiled coili440 – 47233Sequence analysisAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi222 – 353132Gln-richAdd
BLAST
Compositional biasi885 – 8884Poly-Gly

Sequence similaritiesi

Contains 1 Zn(2)-C6 fungal-type DNA-binding domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

GeneTreeiENSGT00390000013273.
HOGENOMiHOG000001044.
InParanoidiQ12151.
OMAiKSKNGCD.
OrthoDBiEOG7327ZS.

Family and domain databases

Gene3Di4.10.240.10. 1 hit.
InterProiIPR021858. Fun_TF.
IPR001138. Zn2-C6_fun-type_DNA-bd.
[Graphical view]
PfamiPF11951. Fungal_trans_2. 1 hit.
PF00172. Zn_clus. 1 hit.
[Graphical view]
SMARTiSM00066. GAL4. 1 hit.
[Graphical view]
SUPFAMiSSF57701. SSF57701. 1 hit.
PROSITEiPS00463. ZN2_CY6_FUNGAL_1. 1 hit.
PS50048. ZN2_CY6_FUNGAL_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q12151-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSEVGIQNHK KAVTKPRRRE KVIELIEVDG KKVSTTSTGK RKFHNKSKNG
60 70 80 90 100
CDNCKRRRVK CDEGKPACRK CTNMKLECQY TPIHLRKGRG ATVVKYVTRK
110 120 130 140 150
ADGSVESDSS VDLPPTIKKE QTPFNDIQSA VKASGSSNDS FPSSASTTKS
160 170 180 190 200
ESEEKSSAPI EDKNNMTPLS MGLQGTINKK DMMNNFFSQN GTIGFGSPER
210 220 230 240 250
LNSGIDGLLL PPLPSGNMGA FQLQQQQQVQ QQSQPQTQAQ QASGTPNERY
260 270 280 290 300
GSFDLAGSPA LQSTGMSLSN SLSGMLLCNR IPSGQNYTQQ QLQYQLHQQL
310 320 330 340 350
QLQQHQQVQL QQYQQLRQEQ HQQVQQQQQE QLQQYQQHFL QQQQQVLLQQ
360 370 380 390 400
EQQPNDEEGG VQEENSKKVK EGPLQSQTSE TTLNSDAATL QADALSQLSK
410 420 430 440 450
MGLSLKSLST FPTAGIGGVS YDFQELLGIK FPINNGNSRA TKASNAEEAL
460 470 480 490 500
ANMQEHHERA AASVKENDGQ LSDTKSPAPS NNAQGGSASI MEPQAADAVS
510 520 530 540 550
TMAPISMIER NMNRNSNISP STPSAVLNDR QEMQDSISSL GNLTKAALEN
560 570 580 590 600
NEPTISLQTS QTENEDDASR QDMTSKINNE ADRSSVSAGT SNIAKLLDLS
610 620 630 640 650
TKGNLNLIDM KLFHHYCTKV WPTITAAKVS GPEIWRDYIP ELAFDYPFLM
660 670 680 690 700
HALLAFSATH LSRTETGLEQ YVSSHRLDAL RLLREAVLEI SENNTDALVA
710 720 730 740 750
SALILIMDSL ANASGNGTVG NQSLNSMSPS AWIFHVKGAA TILTAVWPLS
760 770 780 790 800
ERSKFHNIIS VDLSDLGDVI NPDVGTITEL VCFDESIADL YPVGLDSPYL
810 820 830 840 850
ITLAYLDKLH REKNQGDFIL RVFTFPALLD KTFLALLMTG DLGAMRIMRS
860 870 880 890 900
YYKLLRGFAT EVKDKVWFLE GVTQVLPQDV DEYSGGGGMH MMLDFLGGGL
910
PSMTTTNFSD FSL
Length:913
Mass (Da):100,340
Last modified:November 1, 1996 - v1
Checksum:i6937B82B5F8B2D63
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z68194 Genomic DNA. Translation: CAA92356.1.
Z68195 Genomic DNA. Translation: CAA92364.1.
BK006938 Genomic DNA. Translation: DAA12057.1.
PIRiS61580.
RefSeqiNP_010499.1. NM_001180521.1.

Genome annotation databases

EnsemblFungiiYDR213W; YDR213W; YDR213W.
GeneIDi851799.
KEGGisce:YDR213W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z68194 Genomic DNA. Translation: CAA92356.1.
Z68195 Genomic DNA. Translation: CAA92364.1.
BK006938 Genomic DNA. Translation: DAA12057.1.
PIRiS61580.
RefSeqiNP_010499.1. NM_001180521.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4N9NX-ray2.90A/B598-714[»]
A/B726-878[»]
ProteinModelPortaliQ12151.
SMRiQ12151. Positions 604-878.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi32267. 96 interactions.
DIPiDIP-6262N.
MINTiMINT-2786657.

PTM databases

iPTMnetiQ12151.

Proteomic databases

MaxQBiQ12151.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYDR213W; YDR213W; YDR213W.
GeneIDi851799.
KEGGisce:YDR213W.

Organism-specific databases

EuPathDBiFungiDB:YDR213W.
SGDiS000002621. UPC2.

Phylogenomic databases

GeneTreeiENSGT00390000013273.
HOGENOMiHOG000001044.
InParanoidiQ12151.
OMAiKSKNGCD.
OrthoDBiEOG7327ZS.

Enzyme and pathway databases

BioCyciYEAST:G3O-29795-MONOMER.

Miscellaneous databases

NextBioi969635.
PROiQ12151.

Family and domain databases

Gene3Di4.10.240.10. 1 hit.
InterProiIPR021858. Fun_TF.
IPR001138. Zn2-C6_fun-type_DNA-bd.
[Graphical view]
PfamiPF11951. Fungal_trans_2. 1 hit.
PF00172. Zn_clus. 1 hit.
[Graphical view]
SMARTiSM00066. GAL4. 1 hit.
[Graphical view]
SUPFAMiSSF57701. SSF57701. 1 hit.
PROSITEiPS00463. ZN2_CY6_FUNGAL_1. 1 hit.
PS50048. ZN2_CY6_FUNGAL_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV."
    Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T.
    , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P.
    Nature 387:75-78(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. "Pleiotropic mutations in Saccharomyces cerevisiae affecting sterol uptake and metabolism."
    Lewis T.L., Keesler G.A., Fenner G.P., Parks L.W.
    Yeast 4:93-106(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: MUTAGENESIS OF GLY-888.
  4. "A mutation in a purported regulatory gene affects control of sterol uptake in Saccharomyces cerevisiae."
    Crowley J.H., Leak F.W. Jr., Shianna K.V., Tove S., Parks L.W.
    J. Bacteriol. 180:4177-4183(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: MUTAGENESIS OF GLY-888.
  5. "Regulatory mechanisms controlling expression of the DAN/TIR mannoprotein genes during anaerobic remodeling of the cell wall in Saccharomyces cerevisiae."
    Abramova N.E., Cohen B.D., Sertil O., Kapoor R., Davies K.J., Lowry C.V.
    Genetics 157:1169-1177(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INDUCTION, MUTAGENESIS OF GLY-888.
  6. "Reciprocal regulation of anaerobic and aerobic cell wall mannoprotein gene expression in Saccharomyces cerevisiae."
    Abramova N.E., Sertil O., Mehta S., Lowry C.V.
    J. Bacteriol. 183:2881-2887(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  7. "Upc2p and Ecm22p, dual regulators of sterol biosynthesis in Saccharomyces cerevisiae."
    Vik A., Rine J.
    Mol. Cell. Biol. 21:6395-6405(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  8. "Transcriptional profiling identifies two members of the ATP-binding cassette transporter superfamily required for sterol uptake in yeast."
    Wilcox L.J., Balderes D.A., Wharton B., Tinkelenberg A.H., Rao G., Sturley S.L.
    J. Biol. Chem. 277:32466-32472(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  9. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  10. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  11. "Transcriptional regulation of YML083c under aerobic and anaerobic conditions."
    Ter Linde J.J., Regnacq M., Steensma H.Y.
    Yeast 20:439-454(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  12. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-122, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  13. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-122 AND SER-519, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiUPC2_YEAST
AccessioniPrimary (citable) accession number: Q12151
Secondary accession number(s): D6VSJ7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 2005
Last sequence update: November 1, 1996
Last modified: May 11, 2016
This is version 124 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 752 molecules/cell in log phase SD medium.1 Publication
ECM22 and UPC2 (in combination) null mutants are not viable.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.