ID   PER33_YEAST             Reviewed;         273 AA.
AC   Q12144; D6VY65;
DT   25-JUL-2006, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   27-MAR-2024, entry version 145.
DE   RecName: Full=Pore and endoplasmic reticulum protein of 33 kDa;
GN   Name=PER33; OrderedLocusNames=YLR064W; ORFNames=L2177;
OS   Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX   NCBI_TaxID=559292;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=9169871;
RA   Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W.,
RA   Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A.,
RA   Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K.,
RA   Heuss-Neitzel D., Hilbert H., Hilger F., Kleine K., Koetter P., Louis E.J.,
RA   Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S.,
RA   Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D.,
RA   Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M.,
RA   Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P.,
RA   Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M.,
RA   Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K.,
RA   Zollner A., Hani J., Hoheisel J.D.;
RT   "The nucleotide sequence of Saccharomyces cerevisiae chromosome XII.";
RL   Nature 387:87-90(1997).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=24374639; DOI=10.1534/g3.113.008995;
RA   Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA   Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA   Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT   "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL   G3 (Bethesda) 4:389-398(2014).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=17322287; DOI=10.1101/gr.6037607;
RA   Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA   Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA   Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA   Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA   LaBaer J.;
RT   "Approaching a complete repository of sequence-verified protein-encoding
RT   clones for Saccharomyces cerevisiae.";
RL   Genome Res. 17:536-543(2007).
RN   [4]
RP   SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX   PubMed=14562095; DOI=10.1038/nature02026;
RA   Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W.,
RA   Weissman J.S., O'Shea E.K.;
RT   "Global analysis of protein localization in budding yeast.";
RL   Nature 425:686-691(2003).
RN   [5]
RP   LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX   PubMed=14562106; DOI=10.1038/nature02046;
RA   Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA   O'Shea E.K., Weissman J.S.;
RT   "Global analysis of protein expression in yeast.";
RL   Nature 425:737-741(2003).
RN   [6]
RP   TOPOLOGY [LARGE SCALE ANALYSIS].
RC   STRAIN=ATCC 208353 / W303-1A;
RX   PubMed=16847258; DOI=10.1073/pnas.0604075103;
RA   Kim H., Melen K., Oesterberg M., von Heijne G.;
RT   "A global topology map of the Saccharomyces cerevisiae membrane proteome.";
RL   Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006).
RN   [7]
RP   SUBCELLULAR LOCATION.
RX   PubMed=20498018; DOI=10.1083/jcb.200910043;
RA   Chadrin A., Hess B., San Roman M., Gatti X., Lombard B., Loew D.,
RA   Barral Y., Palancade B., Doye V.;
RT   "Pom33, a novel transmembrane nucleoporin required for proper nuclear pore
RT   complex distribution.";
RL   J. Cell Biol. 189:795-811(2010).
CC   -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane; Multi-pass
CC       membrane protein. Nucleus, nuclear pore complex.
CC   -!- MISCELLANEOUS: Present with 2240 molecules/cell in log phase SD medium.
CC       {ECO:0000269|PubMed:14562106}.
CC   -!- SIMILARITY: Belongs to the PER33/POM33 family. {ECO:0000305}.
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DR   EMBL; X94607; CAA64310.1; -; Genomic_DNA.
DR   EMBL; Z73236; CAA97620.1; -; Genomic_DNA.
DR   EMBL; AY692677; AAT92696.1; -; Genomic_DNA.
DR   EMBL; BK006945; DAA09381.1; -; Genomic_DNA.
DR   PIR; S61637; S61637.
DR   RefSeq; NP_013165.1; NM_001181951.1.
DR   AlphaFoldDB; Q12144; -.
DR   SMR; Q12144; -.
DR   BioGRID; 31338; 247.
DR   DIP; DIP-4778N; -.
DR   IntAct; Q12144; 3.
DR   MINT; Q12144; -.
DR   STRING; 4932.YLR064W; -.
DR   MaxQB; Q12144; -.
DR   PaxDb; 4932-YLR064W; -.
DR   PeptideAtlas; Q12144; -.
DR   EnsemblFungi; YLR064W_mRNA; YLR064W; YLR064W.
DR   GeneID; 850753; -.
DR   KEGG; sce:YLR064W; -.
DR   AGR; SGD:S000004054; -.
DR   SGD; S000004054; PER33.
DR   VEuPathDB; FungiDB:YLR064W; -.
DR   eggNOG; KOG4002; Eukaryota.
DR   GeneTree; ENSGT00390000011368; -.
DR   HOGENOM; CLU_065417_3_0_1; -.
DR   InParanoid; Q12144; -.
DR   OMA; PQFYWFL; -.
DR   OrthoDB; 2045703at2759; -.
DR   BioCyc; YEAST:G3O-32217-MONOMER; -.
DR   BioGRID-ORCS; 850753; 0 hits in 10 CRISPR screens.
DR   PRO; PR:Q12144; -.
DR   Proteomes; UP000002311; Chromosome XII.
DR   RNAct; Q12144; Protein.
DR   GO; GO:0005783; C:endoplasmic reticulum; IDA:SGD.
DR   GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0005635; C:nuclear envelope; IDA:SGD.
DR   GO; GO:0005643; C:nuclear pore; IEA:UniProtKB-SubCell.
DR   GO; GO:0071786; P:endoplasmic reticulum tubular network organization; IBA:GO_Central.
DR   GO; GO:0061024; P:membrane organization; IBA:GO_Central.
DR   GO; GO:0051028; P:mRNA transport; IEA:UniProtKB-KW.
DR   GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR   InterPro; IPR005344; TMEM33/Pom33.
DR   PANTHER; PTHR12703; TRANSMEMBRANE PROTEIN 33; 1.
DR   PANTHER; PTHR12703:SF4; TRANSMEMBRANE PROTEIN 33; 1.
DR   Pfam; PF03661; TMEM33_Pom33; 1.
PE   1: Evidence at protein level;
KW   Endoplasmic reticulum; Membrane; mRNA transport; Nuclear pore complex;
KW   Nucleus; Protein transport; Reference proteome; Translocation;
KW   Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..273
FT                   /note="Pore and endoplasmic reticulum protein of 33 kDa"
FT                   /id="PRO_0000247231"
FT   TOPO_DOM        1..24
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        25..45
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        46..56
FT                   /note="Lumenal"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        57..74
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        75..98
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        99..119
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        120..126
FT                   /note="Lumenal"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        127..147
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        148..176
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        177..197
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        198..199
FT                   /note="Lumenal"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        200..220
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        221..273
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   273 AA;  32637 MW;  F6B7E3A3D1D74F38 CRC64;
     MTVPRNRPMA PFGTIIKSRI KQPQFYWFIG HFLTIFNFIQ FHLSITSKQN QLSCYRRSLF
     YISVTYAIVL YQFFKSDQLK FNFTLLRQEM KKLDNLQYFA MLFILFLLSQ FNIIISGSLY
     SPVIFSIFHF LNYFKENLLP FLPLIPLNLK NLLNSKITVF IQNYNGFFLQ MAQVFEIICG
     LRVGLFLVPF NFFLLLVRRA NVSFEVVGTM LAGLTYVWFF KLRYLQSESM RQIFKQYVLR
     LDAYVSRTLP PYCSRLWNGY KNFVMTVFWK IPV
//