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Protein

Kinetochore-associated protein NSL1

Gene

NSL1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Acts as essential component of the kinetochore MIND complex, which is required for the spindle checkpoint and kinetochore integrity. MIND plays a role in establishing a bipolar spindle-kinetochore interaction by joining kinetochore subunits contacting DNA to those contacting microtubules. NSL1 facilitates the attachment of two of the DASH complex components, DAD2 and SPC19, to the kinetochore in a microtubule-dependent manner.3 Publications

GO - Biological processi

  • cell division Source: UniProtKB-KW
  • chromosome segregation Source: SGD
  • mitotic sister chromatid segregation Source: GO_Central
Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cell division, Mitosis

Enzyme and pathway databases

BioCyciYEAST:G3O-34120-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Kinetochore-associated protein NSL1
Gene namesi
Name:NSL1
Ordered Locus Names:YPL233W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XVI

Organism-specific databases

EuPathDBiFungiDB:YPL233W.
SGDiS000006154. NSL1.

Subcellular locationi

GO - Cellular componenti

  • kinetochore Source: SGD
  • nuclear MIS12/MIND complex Source: SGD
  • spindle pole Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Centromere, Chromosome, Kinetochore, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00000579652 – 216Kinetochore-associated protein NSL1Add BLAST215

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineCombined sources1

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiQ12143.
PRIDEiQ12143.

PTM databases

iPTMnetiQ12143.

Interactioni

Subunit structurei

Component of the MIND kinetochore complex, which is composed of at least MTW1, NNF1, NSL1 and DSN1.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
DSN1P4056813EBI-33666,EBI-25398

Protein-protein interaction databases

BioGridi35929. 85 interactors.
DIPiDIP-5393N.
IntActiQ12143. 24 interactors.
MINTiMINT-558845.

Structurei

3D structure databases

ProteinModelPortaliQ12143.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Phylogenomic databases

HOGENOMiHOG000000975.
InParanoidiQ12143.
KOiK11561.
OMAiQEWEDET.
OrthoDBiEOG092C4MTY.

Family and domain databases

InterProiIPR013950. Kinetochore_Mis14.
[Graphical view]
PfamiPF08641. Mis14. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q12143-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSQGQSKKLD VTVEQLRSIY HQFHDILEEK TDLHLPKKEY DDDAVRREVQ
60 70 80 90 100
IQLQEFLLSA MTMASKSLEV VNADTVGKTV KQLIMESQEK YMEPFDLDLN
110 120 130 140 150
EQVRKMYQEW EDETVKVAQL RQTGPAKINE VYNNSKDEYL AQLDGRIGVL
160 170 180 190 200
QARMMQQQSA DHDDSTDDAD DHINWEHIKQ DYVASLNELY QTQQDLPKVR
210
YNVEKVKRLM DFLEED
Length:216
Mass (Da):25,416
Last modified:November 1, 1996 - v1
Checksum:iBE627C405B4831A5
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti212F → L in AAT93247 (PubMed:17322287).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z73589 Genomic DNA. Translation: CAA97950.1.
X94561 Genomic DNA. Translation: CAA64254.1.
AY693228 Genomic DNA. Translation: AAT93247.1.
BK006949 Genomic DNA. Translation: DAA11203.1.
PIRiS61701.
RefSeqiNP_015091.1. NM_001184047.1.

Genome annotation databases

EnsemblFungiiYPL233W; YPL233W; YPL233W.
GeneIDi855843.
KEGGisce:YPL233W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z73589 Genomic DNA. Translation: CAA97950.1.
X94561 Genomic DNA. Translation: CAA64254.1.
AY693228 Genomic DNA. Translation: AAT93247.1.
BK006949 Genomic DNA. Translation: DAA11203.1.
PIRiS61701.
RefSeqiNP_015091.1. NM_001184047.1.

3D structure databases

ProteinModelPortaliQ12143.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi35929. 85 interactors.
DIPiDIP-5393N.
IntActiQ12143. 24 interactors.
MINTiMINT-558845.

PTM databases

iPTMnetiQ12143.

Proteomic databases

MaxQBiQ12143.
PRIDEiQ12143.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYPL233W; YPL233W; YPL233W.
GeneIDi855843.
KEGGisce:YPL233W.

Organism-specific databases

EuPathDBiFungiDB:YPL233W.
SGDiS000006154. NSL1.

Phylogenomic databases

HOGENOMiHOG000000975.
InParanoidiQ12143.
KOiK11561.
OMAiQEWEDET.
OrthoDBiEOG092C4MTY.

Enzyme and pathway databases

BioCyciYEAST:G3O-34120-MONOMER.

Miscellaneous databases

PROiQ12143.

Family and domain databases

InterProiIPR013950. Kinetochore_Mis14.
[Graphical view]
PfamiPF08641. Mis14. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNSL1_YEAST
AccessioniPrimary (citable) accession number: Q12143
Secondary accession number(s): D6W3D7, Q6B152
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 2005
Last sequence update: November 1, 1996
Last modified: November 30, 2016
This is version 118 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 3710 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  2. Yeast chromosome XVI
    Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.