Autophagy-related protein 9 - Saccharomyces cerevisiae (Baker's yeast)

Names and origin

Protein names

Recommended name:
Autophagy-related protein 9
Alternative name(s):
Cytoplasm to vacuole targeting protein 7

Gene names

Name:	ATG9
Synonyms:	APG9, AUT9, CVT7
Ordered Locus Names:	YDL149W
ORF Names:	D1560

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

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Protein attributes

Sequence length	997 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Function	Involved in autophagy and cytoplasm to vacuole transport (Cvt) vesicle formation. Recruites ATG23 and ATG8 to the pre-autophagosomal structure. Ref.3 Ref.4 Ref.5 Ref.6 Ref.7 Ref.8 Ref.11
Subunit structure	Interacts with ATG18, ATG2 and ATG23. Ref.11 Ref.9 Ref.12
Subcellular location	Cytoplasmic vesicle membrane; Multi-pass membrane protein. Preautophagosomal structure membrane; Multi-pass membrane protein. Note: The proper trafficking of ATG9 between the pre-autophagosomal structure and the other cytoplasmic vesicles requires ATG2, ATG18, ATG23, the ATG1-ATG13 complex and the phosphatidylinositol 3-kinase complex I. Ref.6 Ref.7 Ref.12
Sequence similarities	Belongs to the ATG9 family.

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Ontologies

Keywords
Biological process	Autophagy Protein transport Transport
Cellular component	Cytoplasmic vesicle Membrane
Domain	Transmembrane
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	CVT pathway Ref.7 Inferred from mutant phenotype. Source: SGD autophagic vacuole formation Ref.7 Inferred from mutant phenotype. Source: SGD piecemeal microautophagy of nucleus Inferred from mutant phenotype. Source: SGD protein homooligomerization Inferred from direct assay. Source: SGD
Cellular component	cytoplasmic vesicle membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW membrane fraction Ref.7 Inferred from direct assay. Source: SGD mitochondrion Inferred from direct assay. Source: SGD pre-autophagosomal structure membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 997

997

Autophagy-related protein 9

PRO_0000119839

Regions

Topological domain

1 – 318

318

Cytoplasmic Probable

Transmembrane

319 – 339

21

Potential

Topological domain

340 – 376

37

Lumenal Probable

Transmembrane

377 – 397

21

Potential

Topological domain

398 – 538

141

Cytoplasmic Probable

Transmembrane

539 – 559

21

Potential

Topological domain

560 – 620

61

Lumenal Probable

Transmembrane

621 – 641

21

Potential

Topological domain

642 – 656

15

Cytoplasmic Probable

Transmembrane

657 – 677

21

Potential

Topological domain

678 – 723

46

Lumenal Probable

Transmembrane

724 – 744

21

Potential

Topological domain

745 – 997

253

Cytoplasmic Probable

Compositional bias

907 – 978

72

Asn-rich

Amino acid modifications

Modified residue

111

1

Phosphothreonine Ref.14

Modified residue

112

1

Phosphoserine Ref.14

Modified residue

787

1

Phosphoserine Ref.14

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Sequences

Sequence

Length

Mass (Da)

Tools

Q12142-1 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: E900AD5E3AC5D111
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FASTA

997

115,403

        10         20         30         40         50         60 
MERDEYQLPN SHGKNTFLSR IFGLQSDEVN PSLNSQEMSN FPLPDIERGS SLLHSTNDSR 

        70         80         90        100        110        120 
EDVDENDLRV PESDQGTSTE EEDEVDEEQV QAYAPQISDG LDGDHQLNSV TSKENVLETE 

       130        140        150        160        170        180 
KSNLERLVEG STDDSVPKVG QLSSEEEEDN EFINNDGFDD DTPLFQKSKI HEFSSKKSNT 

       190        200        210        220        230        240 
IEDGKRPLFF RHILQNNRPQ RDTQKLFTSS NAIHHDKDKS ANNGPRNING NQKHGTKYFG 

       250        260        270        280        290        300 
SATQPRFTGS PLNNTNRFTK LFPLRKPNLL SNISVLNNTP EDRINTLSVK ERALWKWANV 

       310        320        330        340        350        360 
ENLDIFLQDV YNYYLGNGFY CIILEKILNI CTLLFVVFVS TYMGHCVDYS KLPTSHRVSD 

       370        380        390        400        410        420 
IIIDKCYSNS ITGFTKFFLW MFYFFVILKI VQLYFDVQKL SELQNFYKYL LNISDDELQT 

       430        440        450        460        470        480 
LPWQNVIQQL MYLKDQNAMT ANVVEVKAKN RIDAHDVANR IMRRENYLIA LYNSDILNLS 

       490        500        510        520        530        540 
LPIPLFRTNV LTKTLEWNIN LCVMGFVFNE SGFIKQSILK PSQREFTREE LQKRFMLAGF 

       550        560        570        580        590        600 
LNIILAPFLV TYFVLLYFFR YFNEYKTSPG SIGARQYTPI AEWKFREYNE LYHIFKKRIS 

       610        620        630        640        650        660 
LSTTLANKYV DQFPKEKTNL FLKFVSFICG SFVAILAFLT VFDPENFLNF EITSDRSVIF 

       670        680        690        700        710        720 
YITILGAIWS VSRNTITQEY HVFDPEETLK ELYEYTHYLP KEWEGRYHKE EIKLEFCKLY 

       730        740        750        760        770        780 
NLRIVILLRE LTSLMITPFV LWFSLPSSAG RIVDFFRENS EYVDGLGYVC KYAMFNMKNI 

       790        800        810        820        830        840 
DGEDTHSMDE DSLTKKIAVN GSHTLNSKRR SKFTAEDHSD KDLANNKMLQ SYVYFMDDYS 

       850        860        870        880        890        900 
NSENLTGKYQ LPAKKGYPNN EGDSFLNNKY SWRKQFQPGQ KPELFRIGKH ALGPGHNISP 

       910        920        930        940        950        960 
AIYSTRNPGK NWDNNNNGDD IKNGTNNATA KNDDNNGNND HEYVLTESFL DSGAFPNHDV 

       970        980        990 
IDHNKMLNSN YNGNGILNKG GVLGLVKEYY KKSDVGR

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References

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[1]	"Analysis of a 23 kb region on the left arm of yeast chromosome IV." Delaveau T.T.D., Blugeon C., Jacq C., Perea J. Yeast 12:1587-1592(1996) [PubMed: 8972581] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome IV." Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T. Zaccaria P. Nature 387:75-78(1997) [PubMed: 9169867] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	"Isolation and characterization of autophagy-defective mutants of Saccharomyces cerevisiae." Tsukada M., Ohsumi Y. FEBS Lett. 333:169-174(1993) [PubMed: 8224160] [Abstract] Cited for: FUNCTION.
[4]	"Isolation and characterization of yeast mutants in the cytoplasm to vacuole protein targeting pathway." Harding T.M., Morano K.A., Scott S.V., Klionsky D.J. J. Cell Biol. 131:591-602(1995) [PubMed: 7593182] [Abstract] Cited for: FUNCTION.
[5]	"Genetic and phenotypic overlap between autophagy and the cytoplasm to vacuole protein targeting pathway." Harding T.M., Hefner-Gravink A., Thumm M., Klionsky D.J. J. Biol. Chem. 271:17621-17624(1996) [PubMed: 8663607] [Abstract] Cited for: FUNCTION.
[6]	"Autophagy and the cvt pathway both depend on AUT9." Lang T., Reiche S., Straub M., Bredschneider M., Thumm M. J. Bacteriol. 182:2125-2133(2000) [PubMed: 10735854] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION.
[7]	"Apg9p/Cvt7p is an integral membrane protein required for transport vesicle formation in the Cvt and autophagy pathways." Noda T., Kim J., Huang W.-P., Baba M., Tokunaga C., Ohsumi Y., Klionsky D.J. J. Cell Biol. 148:465-480(2000) [PubMed: 10662773] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION.
[8]	"The pre-autophagosomal structure organized by concerted functions of APG genes is essential for autophagosome formation." Suzuki K., Kirisako T., Kamada Y., Mizushima N., Noda T., Ohsumi Y. EMBO J. 20:5971-5981(2001) [PubMed: 11689437] [Abstract] Cited for: FUNCTION.
[9]	"Apg2 is a novel protein required for the cytoplasm to vacuole targeting, autophagy, and pexophagy pathways." Wang C.-W., Kim J., Huang W.-P., Abeliovich H., Stromhaug P.E., Dunn W.A. Jr., Klionsky D.J. J. Biol. Chem. 276:30442-30451(2001) [PubMed: 11382760] [Abstract] Cited for: INTERACTION WITH ATG2.
[10]	"A unified nomenclature for yeast autophagy-related genes." Klionsky D.J., Cregg J.M., Dunn W.A. Jr., Emr S.D., Sakai Y., Sandoval I.V., Sibirny A., Subramani S., Thumm M., Veenhuis M., Ohsumi Y. Dev. Cell 5:539-545(2003) [PubMed: 14536056] [Abstract] Cited for: NOMENCLATURE.
[11]	"Atg23 is essential for the cytoplasm to vacuole targeting pathway and efficient autophagy but not pexophagy." Tucker K.A., Reggiori F., Dunn W.A. Jr., Klionsky D.J. J. Biol. Chem. 278:48445-48452(2003) [PubMed: 14504273] [Abstract] Cited for: FUNCTION, INTERACTION WITH ATG23.
[12]	"The Atg1-Atg13 complex regulates Atg9 and Atg23 retrieval transport from the pre-autophagosomal structure." Reggiori F., Tucker K.A., Stromhaug P.E., Klionsky D.J. Dev. Cell 6:79-90(2004) [PubMed: 14723849] [Abstract] Cited for: SUBCELLULAR LOCATION, INTERACTION WITH ATG18, TRAFFICKING.
[13]	"A global topology map of the Saccharomyces cerevisiae membrane proteome." Kim H., Melen K., Oesterberg M., von Heijne G. Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed: 16847258] [Abstract] Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
[14]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-111; SER-112 AND SER-787, MASS SPECTROMETRY.
+	Additional computationally mapped references.

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Cross-references

Sequence databases
	X97751 Genomic DNA. Translation: CAA66342.1. Z74197 Genomic DNA. Translation: CAA98723.1.
PIR	S67697.
RefSeq	NP_010132.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:1938N.
IntAct	Q12142. 90 interactions.
Genome annotation databases
Ensembl	YDL149W. Saccharomyces cerevisiae. [Contig view]
GeneID	851406.
GenomeReviews	Gene locus YDL149W in contig Z71256_GR.
KEGG	sce:YDL149W.
NMPDR	fig\|4932.3.peg.869.
Organism-specific databases
CYGD	YDL149w.
SGD	S000002308. ATG9.
Yeast-GFP	Search...
Phylogenomic databases
HOGENOM	Q12142.
OMA	Q12142. EYTHYLP.
Gene expression databases
ArrayExpress	Q12142.
GermOnline	YDL149W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR007241. Autophagy-rel_prot_9. [Graphical view]
PANTHER	PTHR13038. APG9. 1 hit.
Pfam	PF04109. APG9. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	968579.

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Entry information

Entry name

ATG9_YEAST

Accession

Primary (citable) accession number: Q12142

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 15, 2005
Last sequence update:	November 1, 1996
Last modified:	June 16, 2009
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)

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Relevant documents

SIMILARITY comments

Index of protein domains and families

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other Resources

Entry information

Relevant documents