ID   SAS10_YEAST             Reviewed;         610 AA.
AC   Q12136; Q05842;
DT   07-DEC-2004, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   16-JUN-2009, entry version 66.
DE   RecName: Full=Something about silencing protein 10;
DE   AltName: Full=U3 small nucleolar RNA-associated protein 3;
DE   AltName: Full=U3 snoRNA-associated protein 11;
DE   AltName: Full=U three protein 3;
GN   Name=SAS10; Synonyms=UTP3; OrderedLocusNames=YDL153C; ORFNames=D1545;
OS   Saccharomyces cerevisiae (Baker's yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina;
OC   Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX   NCBI_TaxID=4932;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], OVEREXPRESSION, AND SUBCELLULAR LOCATION.
RX   PubMed=9611201;
RA   Kamakaka R.T., Rine J.;
RT   "Sir- and silencer-independent disruption of silencing in
RT   Saccharomyces by Sas10p.";
RL   Genetics 149:903-914(1998).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   MEDLINE=97127830; PubMed=8972581;
RX   DOI=10.1002/(SICI)1097-0061(199612)12:15<1587::AID-YEA46>3.0.CO;2-6;
RA   Delaveau T.T.D., Blugeon C., Jacq C., Perea J.;
RT   "Analysis of a 23 kb region on the left arm of yeast chromosome IV.";
RL   Yeast 12:1587-1592(1996).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   MEDLINE=97313263; PubMed=9169867;
RA   Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G.,
RA   Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C.,
RA   Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F.,
RA   Delaveau T., del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M.,
RA   Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T.,
RA   Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C.,
RA   Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S.,
RA   Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N.,
RA   Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M.,
RA   Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L.,
RA   Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M.,
RA   Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S.,
RA   Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M.,
RA   Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S.,
RA   Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K.,
RA   Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D.,
RA   Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C.,
RA   Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T.,
RA   Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E.,
RA   Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W.,
RA   Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K.,
RA   Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S.,
RA   Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A.,
RA   Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R.,
RA   Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S.,
RA   Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M.,
RA   Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y.,
RA   Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M.,
RA   Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E.,
RA   Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R.,
RA   Waterston R., Albermann K., Hani J., Heumann K., Kleine K.,
RA   Mewes H.-W., Zollner A., Zaccaria P.;
RT   "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV.";
RL   Nature 387:75-78(1997).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=17322287; DOI=10.1101/gr.6037607;
RA   Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA   Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A.,
RA   Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F.,
RA   Williamson J., Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G.,
RA   Kolodner R.D., LaBaer J.;
RT   "Approaching a complete repository of sequence-verified protein-
RT   encoding clones for Saccharomyces cerevisiae.";
RL   Genome Res. 17:536-543(2007).
RN   [5]
RP   FUNCTION, INTERACTION WITH MPP10 AND SNORNA U3, IDENTIFICATION IN SSU
RP   PROCESSOME BY MASS SPECTROMETRY, AND SUBCELLULAR LOCATION.
RX   MEDLINE=22082292; PubMed=12068309; DOI=10.1038/nature00769;
RA   Dragon F., Gallagher J.E.G., Compagnone-Post P.A., Mitchell B.M.,
RA   Porwancher K.A., Wehner K.A., Wormsley S., Settlage R.E.,
RA   Shabanowitz J., Osheim Y., Beyer A.L., Hunt D.F., Baserga S.J.;
RT   "A large nucleolar U3 ribonucleoprotein required for 18S ribosomal RNA
RT   biogenesis.";
RL   Nature 417:967-970(2002).
RN   [6]
RP   PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-477, AND MASS
RP   SPECTROMETRY.
RX   PubMed=17330950; DOI=10.1021/pr060559j;
RA   Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J.,
RA   Elias J.E., Gygi S.P.;
RT   "Large-scale phosphorylation analysis of alpha-factor-arrested
RT   Saccharomyces cerevisiae.";
RL   J. Proteome Res. 6:1190-1197(2007).
RN   [7]
RP   PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-336 AND SER-339, AND
RP   MASS SPECTROMETRY.
RX   PubMed=17287358; DOI=10.1073/pnas.0607084104;
RA   Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L.,
RA   Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.;
RT   "Analysis of phosphorylation sites on proteins from Saccharomyces
RT   cerevisiae by electron transfer dissociation (ETD) mass
RT   spectrometry.";
RL   Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007).
RN   [8]
RP   PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-314; SER-336; SER-339
RP   AND SER-477, AND MASS SPECTROMETRY.
RX   PubMed=17563356; DOI=10.1073/pnas.0701622104;
RA   Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H.;
RT   "Proteome-wide identification of in vivo targets of DNA damage
RT   checkpoint kinases.";
RL   Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007).
RN   [9]
RP   PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-314; SER-316; SER-336;
RP   SER-339; SER-477 AND SER-483, AND MASS SPECTROMETRY.
RX   PubMed=18407956; DOI=10.1074/mcp.M700468-MCP200;
RA   Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.;
RT   "A multidimensional chromatography technology for in-depth
RT   phosphoproteome analysis.";
RL   Mol. Cell. Proteomics 7:1389-1396(2008).
CC   -!- FUNCTION: Primarily required at the G2/M phase. Essential for
CC       viability: involved in nucleolar processing of pre-18S ribosomal
CC       RNA as part of the ribosomal small subunit (SSU) processome.
CC   -!- SUBUNIT: Interacts with snoRNA U3. Interacts with MPP10. Component
CC       of the ribosomal small subunit (SSU) processome composed of at
CC       least 40 protein subunits and snoRNA U3.
CC   -!- INTERACTION:
CC       P38149:-; NbExp=1; IntAct=EBI-36084, EBI-21176;
CC       P32462:ERG24; NbExp=1; IntAct=EBI-36084, EBI-6502;
CC       P38225:FAT1; NbExp=1; IntAct=EBI-36084, EBI-6819;
CC   -!- SUBCELLULAR LOCATION: Nucleus, nucleolus.
CC   -!- MISCELLANEOUS: When overexpressed, has a role in the structure of
CC       silenced chromatin. Overproduction causes derepression of gene
CC       expression at both HML and HMR, as well as at the rDNA locus and
CC       at telomeres.
CC   -!- SIMILARITY: Belongs to the SAS10 family.
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DR   EMBL; U63063; AAB05801.1; -; mRNA.
DR   EMBL; X97751; CAA66339.1; ALT_INIT; Genomic_DNA.
DR   EMBL; Z74201; CAA98726.1; -; Genomic_DNA.
DR   EMBL; AY692844; AAT92863.1; -; Genomic_DNA.
DR   PIR; S67701; S67701.
DR   RefSeq; NP_010128.1; -.
DR   DIP; DIP:5047N; -.
DR   IntAct; Q12136; 35.
DR   Ensembl; YDL153C; Saccharomyces cerevisiae.
DR   GeneID; 851403; -.
DR   GenomeReviews; Z71256_GR; YDL153C.
DR   KEGG; sce:YDL153C; -.
DR   NMPDR; fig|4932.3.peg.865; -.
DR   CYGD; YDL153c; -.
DR   SGD; S000002312; SAS10.
DR   HOGENOM; Q12136; -.
DR   OMA; Q12136; LRQQKKH.
DR   NextBio; 968573; -.
DR   GermOnline; YDL153C; Saccharomyces cerevisiae.
DR   GO; GO:0005730; C:nucleolus; IDA:SGD.
DR   GO; GO:0032040; C:small-subunit processome; IDA:SGD.
DR   GO; GO:0005515; F:protein binding; IPI:IntAct.
DR   GO; GO:0007049; P:cell cycle; IEA:UniProtKB-KW.
DR   GO; GO:0000480; P:endonucleolytic cleavage in 5'-ETS of trici...; IMP:SGD.
DR   GO; GO:0000447; P:endonucleolytic cleavage in ITS1 to separat...; IMP:SGD.
DR   GO; GO:0000472; P:endonucleolytic cleavage to generate mature...; IMP:SGD.
DR   GO; GO:0016458; P:gene silencing; IEA:InterPro.
DR   InterPro; IPR007146; Sas10/Utp3/C1D.
DR   InterPro; IPR018972; Sas10_C.
DR   Pfam; PF04000; Sas10_Utp3; 1.
DR   Pfam; PF09368; Sas10_Utp3_C; 1.
PE   1: Evidence at protein level;
KW   Cell cycle; Complete proteome; Nucleus; Phosphoprotein;
KW   Ribonucleoprotein; Ribosome biogenesis; rRNA processing.
FT   CHAIN         1    610       Something about silencing protein 10.
FT                                /FTId=PRO_0000114330.
FT   COMPBIAS     75     86       Poly-Glu.
FT   COMPBIAS    167    172       Poly-Glu.
FT   COMPBIAS    360    365       Poly-Glu.
FT   MOD_RES     314    314       Phosphoserine.
FT   MOD_RES     316    316       Phosphoserine.
FT   MOD_RES     336    336       Phosphoserine.
FT   MOD_RES     339    339       Phosphoserine.
FT   MOD_RES     477    477       Phosphoserine.
FT   MOD_RES     483    483       Phosphoserine.
SQ   SEQUENCE   610 AA;  70259 MW;  9278BF873834FC7E CRC64;
     MVRKGSNRTK TSEVGDEINP YGLNEVDDFA SKREKVLLGQ STFGDSNKDD DHSLLEDEDE
     EEVLAMDEDD ESIDEREDEE EEEEEELDGA AAYKKIFGRN LETDQLPEED EENGMLDNEN
     AWGSTKGEYY GADDLDDDEA AKEIEKEALR QQKKHLEELN MNDYLDEEEE EEWVKSAKEF
     DMGEFKNSTK QADTKTSITD ILNMDDEARD NYLRTMFPEF APLSKEFTEL APKFDELKKS
     EENEFNKLKL IALGSYLGTI SCYYSILLHE LHNNEDFTSM KGHPVMEKIL TTKEIWRQAS
     ELPSSFDVNE GDGSESEETA NIEAFNEKKL NELQNSEDSD AEDGGKQKQE IDEEERESDE
     EEEEEDVDID DFEEYVAQSR LHSKPKTSSM PEADDFIESE IADVDAQDKK ARRRTLRFYT
     SKIDQQENKK TDRFKGDDDI PYKERLFERQ QRLLDEARKR GMHDNNGADL DDKDYGSEDE
     AVSRSINTQG ENDYYQQVQR GKQDKKISRK EAHKNAVIAA REGKLAELAE NVSGDGKRAI
     NYQILKNKGL TPKRNKDNRN SRVKKRKKYQ KAQKKLKSVR AVYSGGQSGV YEGEKTGIKK
     GLTRSVKFKN
//