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Protein

Something about silencing protein 10

Gene

SAS10

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Primarily required at the G2/M phase. Essential for viability: involved in nucleolar processing of pre-18S ribosomal RNA as part of the ribosomal small subunit (SSU) processome.1 Publication

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ribonucleoprotein

Keywords - Biological processi

Cell cycle, Ribosome biogenesis, rRNA processing

Enzyme and pathway databases

BioCyciYEAST:G3O-29548-MONOMER.
ReactomeiR-SCE-6790901. rRNA modification in the nucleus and cytosol.
R-SCE-6791226. Major pathway of rRNA processing in the nucleolus and cytosol.

Names & Taxonomyi

Protein namesi
Recommended name:
Something about silencing protein 10
Alternative name(s):
U three protein 3
U3 small nucleolar RNA-associated protein 3
U3 snoRNA-associated protein 11
Gene namesi
Name:SAS10
Synonyms:UTP3
Ordered Locus Names:YDL153C
ORF Names:D1545
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

EuPathDBiFungiDB:YDL153C.
SGDiS000002312. SAS10.

Subcellular locationi

GO - Cellular componenti

  • nucleolus Source: SGD
  • nucleoplasm Source: Reactome
  • nucleus Source: SGD
  • small-subunit processome Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001143301 – 610Something about silencing protein 10Add BLAST610

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei12PhosphoserineCombined sources1
Modified residuei314PhosphoserineCombined sources1
Modified residuei316PhosphoserineCombined sources1
Modified residuei336PhosphoserineCombined sources1
Modified residuei339PhosphoserineCombined sources1
Modified residuei477PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ12136.
PRIDEiQ12136.

PTM databases

iPTMnetiQ12136.

Interactioni

Subunit structurei

Interacts with snoRNA U3. Interacts with MPP10. Component of the ribosomal small subunit (SSU) processome composed of at least 40 protein subunits and snoRNA U3.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
MPP10P470835EBI-36084,EBI-11168
UTP25P404984EBI-36084,EBI-25113

Protein-protein interaction databases

BioGridi31910. 65 interactors.
DIPiDIP-5047N.
IntActiQ12136. 12 interactors.
MINTiMINT-477330.

Structurei

3D structure databases

ProteinModelPortaliQ12136.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi75 – 86Poly-GluAdd BLAST12
Compositional biasi167 – 172Poly-Glu6
Compositional biasi360 – 365Poly-Glu6

Sequence similaritiesi

Belongs to the SAS10 family.Curated

Phylogenomic databases

GeneTreeiENSGT00500000044947.
HOGENOMiHOG000207285.
InParanoidiQ12136.
KOiK14767.
OMAiHEELMVI.
OrthoDBiEOG092C4F9B.

Family and domain databases

InterProiIPR018972. Sas10_C_dom.
[Graphical view]
PfamiPF09368. Sas10. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q12136-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVRKGSNRTK TSEVGDEINP YGLNEVDDFA SKREKVLLGQ STFGDSNKDD
60 70 80 90 100
DHSLLEDEDE EEVLAMDEDD ESIDEREDEE EEEEEELDGA AAYKKIFGRN
110 120 130 140 150
LETDQLPEED EENGMLDNEN AWGSTKGEYY GADDLDDDEA AKEIEKEALR
160 170 180 190 200
QQKKHLEELN MNDYLDEEEE EEWVKSAKEF DMGEFKNSTK QADTKTSITD
210 220 230 240 250
ILNMDDEARD NYLRTMFPEF APLSKEFTEL APKFDELKKS EENEFNKLKL
260 270 280 290 300
IALGSYLGTI SCYYSILLHE LHNNEDFTSM KGHPVMEKIL TTKEIWRQAS
310 320 330 340 350
ELPSSFDVNE GDGSESEETA NIEAFNEKKL NELQNSEDSD AEDGGKQKQE
360 370 380 390 400
IDEEERESDE EEEEEDVDID DFEEYVAQSR LHSKPKTSSM PEADDFIESE
410 420 430 440 450
IADVDAQDKK ARRRTLRFYT SKIDQQENKK TDRFKGDDDI PYKERLFERQ
460 470 480 490 500
QRLLDEARKR GMHDNNGADL DDKDYGSEDE AVSRSINTQG ENDYYQQVQR
510 520 530 540 550
GKQDKKISRK EAHKNAVIAA REGKLAELAE NVSGDGKRAI NYQILKNKGL
560 570 580 590 600
TPKRNKDNRN SRVKKRKKYQ KAQKKLKSVR AVYSGGQSGV YEGEKTGIKK
610
GLTRSVKFKN
Length:610
Mass (Da):70,259
Last modified:November 1, 1996 - v1
Checksum:i9278BF873834FC7E
GO

Sequence cautioni

The sequence CAA66339 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U63063 mRNA. Translation: AAB05801.1.
X97751 Genomic DNA. Translation: CAA66339.1. Different initiation.
Z74201 Genomic DNA. Translation: CAA98726.1.
AY692844 Genomic DNA. Translation: AAT92863.1.
BK006938 Genomic DNA. Translation: DAA11707.1.
PIRiS67701.
RefSeqiNP_010128.1. NM_001180213.1.

Genome annotation databases

EnsemblFungiiYDL153C; YDL153C; YDL153C.
GeneIDi851403.
KEGGisce:YDL153C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U63063 mRNA. Translation: AAB05801.1.
X97751 Genomic DNA. Translation: CAA66339.1. Different initiation.
Z74201 Genomic DNA. Translation: CAA98726.1.
AY692844 Genomic DNA. Translation: AAT92863.1.
BK006938 Genomic DNA. Translation: DAA11707.1.
PIRiS67701.
RefSeqiNP_010128.1. NM_001180213.1.

3D structure databases

ProteinModelPortaliQ12136.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi31910. 65 interactors.
DIPiDIP-5047N.
IntActiQ12136. 12 interactors.
MINTiMINT-477330.

PTM databases

iPTMnetiQ12136.

Proteomic databases

MaxQBiQ12136.
PRIDEiQ12136.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYDL153C; YDL153C; YDL153C.
GeneIDi851403.
KEGGisce:YDL153C.

Organism-specific databases

EuPathDBiFungiDB:YDL153C.
SGDiS000002312. SAS10.

Phylogenomic databases

GeneTreeiENSGT00500000044947.
HOGENOMiHOG000207285.
InParanoidiQ12136.
KOiK14767.
OMAiHEELMVI.
OrthoDBiEOG092C4F9B.

Enzyme and pathway databases

BioCyciYEAST:G3O-29548-MONOMER.
ReactomeiR-SCE-6790901. rRNA modification in the nucleus and cytosol.
R-SCE-6791226. Major pathway of rRNA processing in the nucleolus and cytosol.

Miscellaneous databases

PROiQ12136.

Family and domain databases

InterProiIPR018972. Sas10_C_dom.
[Graphical view]
PfamiPF09368. Sas10. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSAS10_YEAST
AccessioniPrimary (citable) accession number: Q12136
Secondary accession number(s): D6VRJ7, Q05842
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 7, 2004
Last sequence update: November 1, 1996
Last modified: November 2, 2016
This is version 125 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

When overexpressed, has a role in the structure of silenced chromatin. Overproduction causes derepression of gene expression at both HML and HMR, as well as at the rDNA locus and at telomeres.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.