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Q12125

- GET4_YEAST

UniProt

Q12125 - GET4_YEAST

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Protein

Golgi to ER traffic protein 4

Gene

GET4

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

May play a role in insertion of tail-anchored proteins into the endoplasmic reticulum membrane.1 Publication

GO - Biological processi

  1. posttranslational protein targeting to membrane Source: SGD
  2. protein insertion into ER membrane Source: SGD
  3. vesicle-mediated transport Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

ER-Golgi transport, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-33680-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Golgi to ER traffic protein 4
Alternative name(s):
Guided entry of tail-anchored proteins 4
Gene namesi
Name:GET4
Ordered Locus Names:YOR164C
ORF Names:O3580
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XV

Organism-specific databases

CYGDiYOR164c.
SGDiS000005690. GET4.

Subcellular locationi

Cytoplasm 1 Publication

GO - Cellular componenti

  1. TRC complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 312312Golgi to ER traffic protein 4PRO_0000228107Add
BLAST

Proteomic databases

MaxQBiQ12125.
PaxDbiQ12125.
PeptideAtlasiQ12125.

Expressioni

Gene expression databases

GenevestigatoriQ12125.

Interactioni

Subunit structurei

Interacts with MDY2/GET5.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
GET3Q121544EBI-36940,EBI-2989
MDY2Q122858EBI-36940,EBI-34904

Protein-protein interaction databases

BioGridi34560. 76 interactions.
DIPiDIP-2005N.
IntActiQ12125. 9 interactions.
MINTiMINT-396784.
STRINGi4932.YOR164C.

Structurei

Secondary structure

1
312
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi13 – 2614
Helixi29 – 4517
Helixi49 – 6517
Helixi69 – 8517
Helixi92 – 10211
Helixi112 – 12514
Helixi134 – 14613
Helixi150 – 1589
Helixi162 – 17817
Helixi184 – 20017
Helixi204 – 22219
Beta strandi226 – 2316
Beta strandi234 – 2418
Helixi243 – 25715
Helixi260 – 26910
Helixi271 – 2766
Helixi278 – 28912

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2WPVX-ray1.99A/C/E/G1-312[»]
3LKUX-ray2.80A/C/E11-300[»]
4PWXX-ray5.40C/E11-290[»]
ProteinModelPortaliQ12125.
SMRiQ12125. Positions 11-299.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ12125.

Family & Domainsi

Sequence similaritiesi

Belongs to the GET4 family.Curated

Phylogenomic databases

eggNOGiNOG252493.
GeneTreeiENSGT00390000015750.
InParanoidiQ12125.
OMAiRTITNRY.
OrthoDBiEOG7F253S.

Family and domain databases

InterProiIPR007317. UPF0363.
[Graphical view]
PANTHERiPTHR12875. PTHR12875. 1 hit.
PfamiPF04190. DUF410. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q12125-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MVPAESNAVQ AKLAKTLQRF ENKIKAGDYY EAHQTLRTIA NRYVRSKSYE
60 70 80 90 100
HAIELISQGA LSFLKAKQGG SGTDLIFYLL EVYDLAEVKV DDISVARLVR
110 120 130 140 150
LIAELDPSEP NLKDVITGMN NWSIKFSEYK FGDPYLHNTI GSKLLEGDFV
160 170 180 190 200
YEAERYFMLG THDSMIKYVD LLWDWLCQVD DIEDSTVAEF FSRLVFNYLF
210 220 230 240 250
ISNISFAHES KDIFLERFIE KFHPKYEKID KNGYEIVFFE DYSDLNFLQL
260 270 280 290 300
LLITCQTKDK SYFLNLKNHY LDFSQAYKSE LEFLGQEYFN IVAPKQTNFL
310
QDMMSGFLGG SK
Length:312
Mass (Da):36,280
Last modified:November 1, 1996 - v1
Checksum:i98AFFDB4EA51B40F
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U55021 Genomic DNA. Translation: AAB47411.1.
Z75072 Genomic DNA. Translation: CAA99370.1.
AY558036 Genomic DNA. Translation: AAS56362.1.
BK006948 Genomic DNA. Translation: DAA10938.1.
PIRiS67052.
RefSeqiNP_014807.3. NM_001183583.3.

Genome annotation databases

EnsemblFungiiYOR164C; YOR164C; YOR164C.
GeneIDi854335.
KEGGisce:YOR164C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U55021 Genomic DNA. Translation: AAB47411.1 .
Z75072 Genomic DNA. Translation: CAA99370.1 .
AY558036 Genomic DNA. Translation: AAS56362.1 .
BK006948 Genomic DNA. Translation: DAA10938.1 .
PIRi S67052.
RefSeqi NP_014807.3. NM_001183583.3.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2WPV X-ray 1.99 A/C/E/G 1-312 [» ]
3LKU X-ray 2.80 A/C/E 11-300 [» ]
4PWX X-ray 5.40 C/E 11-290 [» ]
ProteinModelPortali Q12125.
SMRi Q12125. Positions 11-299.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 34560. 76 interactions.
DIPi DIP-2005N.
IntActi Q12125. 9 interactions.
MINTi MINT-396784.
STRINGi 4932.YOR164C.

Proteomic databases

MaxQBi Q12125.
PaxDbi Q12125.
PeptideAtlasi Q12125.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YOR164C ; YOR164C ; YOR164C .
GeneIDi 854335.
KEGGi sce:YOR164C.

Organism-specific databases

CYGDi YOR164c.
SGDi S000005690. GET4.

Phylogenomic databases

eggNOGi NOG252493.
GeneTreei ENSGT00390000015750.
InParanoidi Q12125.
OMAi RTITNRY.
OrthoDBi EOG7F253S.

Enzyme and pathway databases

BioCyci YEAST:G3O-33680-MONOMER.

Miscellaneous databases

EvolutionaryTracei Q12125.
NextBioi 976399.
PROi Q12125.

Gene expression databases

Genevestigatori Q12125.

Family and domain databases

InterProi IPR007317. UPF0363.
[Graphical view ]
PANTHERi PTHR12875. PTHR12875. 1 hit.
Pfami PF04190. DUF410. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Analysis of a 22,956 bp region on the right arm of Saccharomyces cerevisiae chromosome XV."
    Madania A., Poch O., Tarassov I.A., Winsor B., Martin R.P.
    Yeast 12:1563-1573(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: S288c / FY1678.
  2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XV."
    Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D.
    , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.
    Nature 387:98-102(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  5. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  7. "Comprehensive characterization of genes required for protein folding in the endoplasmic reticulum."
    Jonikas M.C., Collins S.R., Denic V., Oh E., Quan E.M., Schmid V., Weibezahn J., Schwappach B., Walter P., Weissman J.S., Schuldiner M.
    Science 323:1693-1697(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  8. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  9. "Crystal structure of Get4-Get5 complex and its interactions with Sgt2, Get3, and Ydj1."
    Chang Y.W., Chuang Y.C., Ho Y.C., Cheng M.Y., Sun Y.J., Hsiao C.D., Wang C.
    J. Biol. Chem. 285:9962-9970(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.99 ANGSTROMS) IN COMPLEX WITH MDY2.

Entry informationi

Entry nameiGET4_YEAST
AccessioniPrimary (citable) accession number: Q12125
Secondary accession number(s): D6W2M2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 21, 2006
Last sequence update: November 1, 1996
Last modified: October 29, 2014
This is version 107 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 5350 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Uncharacterized protein families (UPF)
    List of uncharacterized protein family (UPF) entries
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  5. Yeast chromosome XV
    Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

External Data

Dasty 3