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Q12125

- GET4_YEAST

UniProt

Q12125 - GET4_YEAST

Protein

Golgi to ER traffic protein 4

Gene

GET4

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 106 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    May play a role in insertion of tail-anchored proteins into the endoplasmic reticulum membrane.1 Publication

    GO - Molecular functioni

    1. protein binding Source: IntAct

    GO - Biological processi

    1. posttranslational protein targeting to membrane Source: SGD
    2. protein insertion into ER membrane Source: SGD
    3. vesicle-mediated transport Source: UniProtKB-KW

    Keywords - Biological processi

    ER-Golgi transport, Transport

    Enzyme and pathway databases

    BioCyciYEAST:G3O-33680-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Golgi to ER traffic protein 4
    Alternative name(s):
    Guided entry of tail-anchored proteins 4
    Gene namesi
    Name:GET4
    Ordered Locus Names:YOR164C
    ORF Names:O3580
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XV

    Organism-specific databases

    CYGDiYOR164c.
    SGDiS000005690. GET4.

    Subcellular locationi

    Cytoplasm 1 Publication

    GO - Cellular componenti

    1. TRC complex Source: SGD

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 312312Golgi to ER traffic protein 4PRO_0000228107Add
    BLAST

    Proteomic databases

    MaxQBiQ12125.
    PaxDbiQ12125.
    PeptideAtlasiQ12125.

    Expressioni

    Gene expression databases

    GenevestigatoriQ12125.

    Interactioni

    Subunit structurei

    Interacts with MDY2/GET5.1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    GET3Q121544EBI-36940,EBI-2989
    MDY2Q122858EBI-36940,EBI-34904

    Protein-protein interaction databases

    BioGridi34560. 75 interactions.
    DIPiDIP-2005N.
    IntActiQ12125. 9 interactions.
    MINTiMINT-396784.
    STRINGi4932.YOR164C.

    Structurei

    Secondary structure

    1
    312
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi13 – 2614
    Helixi29 – 4517
    Helixi49 – 6517
    Helixi69 – 8517
    Helixi92 – 10211
    Helixi112 – 12514
    Helixi134 – 14613
    Helixi150 – 1589
    Helixi162 – 17817
    Helixi184 – 20017
    Helixi204 – 22219
    Beta strandi226 – 2316
    Beta strandi234 – 2418
    Helixi243 – 25715
    Helixi260 – 26910
    Helixi271 – 2766
    Helixi278 – 28912

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2WPVX-ray1.99A/C/E/G1-312[»]
    3LKUX-ray2.80A/C/E11-300[»]
    4PWXX-ray5.40C/E11-290[»]
    ProteinModelPortaliQ12125.
    SMRiQ12125. Positions 11-299.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ12125.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the GET4 family.Curated

    Phylogenomic databases

    eggNOGiNOG252493.
    GeneTreeiENSGT00390000015750.
    OMAiRTITNRY.
    OrthoDBiEOG7F253S.

    Family and domain databases

    InterProiIPR007317. UPF0363.
    [Graphical view]
    PANTHERiPTHR12875. PTHR12875. 1 hit.
    PfamiPF04190. DUF410. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q12125-1 [UniParc]FASTAAdd to Basket

    « Hide

    MVPAESNAVQ AKLAKTLQRF ENKIKAGDYY EAHQTLRTIA NRYVRSKSYE    50
    HAIELISQGA LSFLKAKQGG SGTDLIFYLL EVYDLAEVKV DDISVARLVR 100
    LIAELDPSEP NLKDVITGMN NWSIKFSEYK FGDPYLHNTI GSKLLEGDFV 150
    YEAERYFMLG THDSMIKYVD LLWDWLCQVD DIEDSTVAEF FSRLVFNYLF 200
    ISNISFAHES KDIFLERFIE KFHPKYEKID KNGYEIVFFE DYSDLNFLQL 250
    LLITCQTKDK SYFLNLKNHY LDFSQAYKSE LEFLGQEYFN IVAPKQTNFL 300
    QDMMSGFLGG SK 312
    Length:312
    Mass (Da):36,280
    Last modified:November 1, 1996 - v1
    Checksum:i98AFFDB4EA51B40F
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U55021 Genomic DNA. Translation: AAB47411.1.
    Z75072 Genomic DNA. Translation: CAA99370.1.
    AY558036 Genomic DNA. Translation: AAS56362.1.
    BK006948 Genomic DNA. Translation: DAA10938.1.
    PIRiS67052.
    RefSeqiNP_014807.3. NM_001183583.3.

    Genome annotation databases

    EnsemblFungiiYOR164C; YOR164C; YOR164C.
    GeneIDi854335.
    KEGGisce:YOR164C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U55021 Genomic DNA. Translation: AAB47411.1 .
    Z75072 Genomic DNA. Translation: CAA99370.1 .
    AY558036 Genomic DNA. Translation: AAS56362.1 .
    BK006948 Genomic DNA. Translation: DAA10938.1 .
    PIRi S67052.
    RefSeqi NP_014807.3. NM_001183583.3.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2WPV X-ray 1.99 A/C/E/G 1-312 [» ]
    3LKU X-ray 2.80 A/C/E 11-300 [» ]
    4PWX X-ray 5.40 C/E 11-290 [» ]
    ProteinModelPortali Q12125.
    SMRi Q12125. Positions 11-299.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 34560. 75 interactions.
    DIPi DIP-2005N.
    IntActi Q12125. 9 interactions.
    MINTi MINT-396784.
    STRINGi 4932.YOR164C.

    Proteomic databases

    MaxQBi Q12125.
    PaxDbi Q12125.
    PeptideAtlasi Q12125.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YOR164C ; YOR164C ; YOR164C .
    GeneIDi 854335.
    KEGGi sce:YOR164C.

    Organism-specific databases

    CYGDi YOR164c.
    SGDi S000005690. GET4.

    Phylogenomic databases

    eggNOGi NOG252493.
    GeneTreei ENSGT00390000015750.
    OMAi RTITNRY.
    OrthoDBi EOG7F253S.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-33680-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei Q12125.
    NextBioi 976399.
    PROi Q12125.

    Gene expression databases

    Genevestigatori Q12125.

    Family and domain databases

    InterProi IPR007317. UPF0363.
    [Graphical view ]
    PANTHERi PTHR12875. PTHR12875. 1 hit.
    Pfami PF04190. DUF410. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Analysis of a 22,956 bp region on the right arm of Saccharomyces cerevisiae chromosome XV."
      Madania A., Poch O., Tarassov I.A., Winsor B., Martin R.P.
      Yeast 12:1563-1573(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: S288c / FY1678.
    2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XV."
      Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D.
      , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.
      Nature 387:98-102(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    5. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    7. "Comprehensive characterization of genes required for protein folding in the endoplasmic reticulum."
      Jonikas M.C., Collins S.R., Denic V., Oh E., Quan E.M., Schmid V., Weibezahn J., Schwappach B., Walter P., Weissman J.S., Schuldiner M.
      Science 323:1693-1697(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    8. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    9. "Crystal structure of Get4-Get5 complex and its interactions with Sgt2, Get3, and Ydj1."
      Chang Y.W., Chuang Y.C., Ho Y.C., Cheng M.Y., Sun Y.J., Hsiao C.D., Wang C.
      J. Biol. Chem. 285:9962-9970(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.99 ANGSTROMS) IN COMPLEX WITH MDY2.

    Entry informationi

    Entry nameiGET4_YEAST
    AccessioniPrimary (citable) accession number: Q12125
    Secondary accession number(s): D6W2M2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 21, 2006
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 106 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 5350 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families
    3. Uncharacterized protein families (UPF)
      List of uncharacterized protein family (UPF) entries
    4. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    5. Yeast chromosome XV
      Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

    External Data

    Dasty 3