Golgi to ER traffic protein 4 - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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Q12125 (GET4_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 29, 2013. Version 98. History...

Clusters with 100%, 90%, 50% identity |

Documents (5) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Golgi to ER traffic protein 4

Alternative name(s):
Guided entry of tail-anchored proteins 4

Gene names

Name:	GET4
Ordered Locus Names:	YOR164C
ORF Names:	O3580

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length	312 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May play a role in insertion of tail-anchored proteins into the endoplasmic reticulum membrane. Ref.7
Subunit structure	Interacts with MDY2/GET5.
Subcellular location	Cytoplasm Ref.5.
Miscellaneous	Present with 5350 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the GET4 family.

Ontologies

Keywords
Biological process	ER-Golgi transport Transport
Cellular component	Cytoplasm
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	posttranslational protein targeting to membrane Inferred from direct assay PubMed 20850366. Source: SGD protein insertion into ER membrane Inferred from genetic interaction Ref.7. Source: SGD vesicle-mediated transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	TRC complex Inferred from direct assay PubMed 20850366. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
GET3	Q12154	4	EBI-36940,EBI-2989
MDY2	Q12285	8	EBI-36940,EBI-34904

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 312

312

Golgi to ER traffic protein 4

PRO_0000228107

Secondary structure

312

Helix Strand Turn

Details...

Sequences

Sequence

Length

Mass (Da)

Tools

Q12125 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 98AFFDB4EA51B40F
Show »

FASTA

312

36,280

        10         20         30         40         50         60 
MVPAESNAVQ AKLAKTLQRF ENKIKAGDYY EAHQTLRTIA NRYVRSKSYE HAIELISQGA 

        70         80         90        100        110        120 
LSFLKAKQGG SGTDLIFYLL EVYDLAEVKV DDISVARLVR LIAELDPSEP NLKDVITGMN 

       130        140        150        160        170        180 
NWSIKFSEYK FGDPYLHNTI GSKLLEGDFV YEAERYFMLG THDSMIKYVD LLWDWLCQVD 

       190        200        210        220        230        240 
DIEDSTVAEF FSRLVFNYLF ISNISFAHES KDIFLERFIE KFHPKYEKID KNGYEIVFFE 

       250        260        270        280        290        300 
DYSDLNFLQL LLITCQTKDK SYFLNLKNHY LDFSQAYKSE LEFLGQEYFN IVAPKQTNFL 

       310 
QDMMSGFLGG SK

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Analysis of a 22,956 bp region on the right arm of Saccharomyces cerevisiae chromosome XV." Madania A., Poch O., Tarassov I.A., Winsor B., Martin R.P. Yeast 12:1563-1573(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: S288c / FY1678.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XV." Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D. Kleine K. Nature 387:98-102(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. LaBaer J. Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[5]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]	"Comprehensive characterization of genes required for protein folding in the endoplasmic reticulum." Jonikas M.C., Collins S.R., Denic V., Oh E., Quan E.M., Schmid V., Weibezahn J., Schwappach B., Walter P., Weissman J.S., Schuldiner M. Science 323:1693-1697(2009) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[8]	"Crystal structure of Get4-Get5 complex and its interactions with Sgt2, Get3, and Ydj1." Chang Y.W., Chuang Y.C., Ho Y.C., Cheng M.Y., Sun Y.J., Hsiao C.D., Wang C. J. Biol. Chem. 285:9962-9970(2010) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.99 ANGSTROMS) IN COMPLEX WITH MDY2.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

U55021 Genomic DNA. Translation: AAB47411.1.
Z75072 Genomic DNA. Translation: CAA99370.1.
AY558036 Genomic DNA. Translation: AAS56362.1.
BK006948 Genomic DNA. Translation: DAA10938.1.

PIR

S67052.

RefSeq

NP_014807.3. NM_001183583.3.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2WPV	X-ray	1.99	A/C/E/G	1-312	[»]
3LKU	X-ray	2.80	A/C/E	11-300	[»]

ProteinModelPortal

Q12125.

SMR

Q12125. Positions 11-299.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-2005N.

IntAct

Q12125. 9 interactions.

MINT

MINT-396784.

STRING

4932.YOR164C.

Proteomic databases

PaxDb

Q12125.

PeptideAtlas

Q12125.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblFungi

YOR164C; YOR164C; YOR164C.

GeneID

854335.

KEGG

sce:YOR164C.

Organism-specific databases

CYGD

YOR164c.

SGD

S000005690. GET4.

Phylogenomic databases

eggNOG

NOG252493.

GeneTree

ENSGT00390000015750.

OMA

TGMNNWS.

OrthoDB

EOG4ZSDCM.

Enzyme and pathway databases

BioCyc

YEAST:G3O-33680-MONOMER.

Gene expression databases

Genevestigator

Q12125.

GermOnline

YOR164C. Saccharomyces cerevisiae.

Family and domain databases

InterPro

IPR007317. UPF0363.
[Graphical view]

PANTHER

PTHR12875. PTHR12875. 1 hit.

Pfam

PF04190. DUF410. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

Q12125.

NextBio

976399.

Entry information

Entry name

GET4_YEAST

Accession

Primary (citable) accession number: Q12125
Secondary accession number(s): D6W2M2

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 21, 2006
Last sequence update:	November 1, 1996
Last modified:	May 29, 2013
This is version 98 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome XV

Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

Uncharacterized protein families (UPF)

List of uncharacterized protein family (UPF) entries

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents