Q12125 (GET4_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Golgi to ER traffic protein 4

Alternative name(s):
Guided entry of tail-anchored proteins 4

Gene names

Name:	GET4
Ordered Locus Names:	YOR164C
ORF Names:	O3580

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	312 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May play a role in insertion of tail-anchored proteins into the endoplasmic reticulum membrane. Ref.7
Subunit structure	Interacts with MDY2/GET5.
Subcellular location	Cytoplasm Ref.5.
Miscellaneous	Present with 5350 molecules/cell in log phase SD medium. Ref.6
Sequence similarities	Belongs to the GET4 family.

Ontologies

Keywords
Biological process	ER-Golgi transport Transport
Cellular component	Cytoplasm
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	posttranslational protein targeting to membrane Inferred from direct assay. Source: SGD protein insertion into ER membrane Inferred from genetic interaction Ref.7. Source: SGD vesicle-mediated transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	TRC complex Inferred from direct assay. Source: SGD
Molecular function	protein binding Inferred from physical interaction Ref.8. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
GET3	Q12154	4	EBI-36940,EBI-2989
MDY2	Q12285	8	EBI-36940,EBI-34904

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 312

312

Golgi to ER traffic protein 4

PRO_0000228107

Secondary structure

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312

Helix Strand Turn

Details...

Helix

13 – 25

13

Helix

29 – 45

17

Helix

49 – 57

9

Helix

61 – 65

5

Helix

74 – 85

12

Helix

92 – 102

11

Helix

112 – 116

5

Helix

120 – 125

6

Helix

134 – 139

6

Turn

143 – 145

3

Helix

150 – 158

9

Helix

162 – 178

17

Helix

184 – 200

17

Helix

204 – 222

19

Beta strand

226 – 230

5

Beta strand

235 – 241

7

Helix

243 – 257

15

Helix

260 – 269

10

Helix

271 – 276

6

Helix

278 – 283

6

Turn

287 – 289

3

Sequences

	Sequence		Length	Mass (Da)
	Q12125 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 98AFFDB4EA51B40F	FASTA	312	36,280
	10 20 30 40 50 60 MVPAESNAVQ AKLAKTLQRF ENKIKAGDYY EAHQTLRTIA NRYVRSKSYE HAIELISQGA 70 80 90 100 110 120 LSFLKAKQGG SGTDLIFYLL EVYDLAEVKV DDISVARLVR LIAELDPSEP NLKDVITGMN 130 140 150 160 170 180 NWSIKFSEYK FGDPYLHNTI GSKLLEGDFV YEAERYFMLG THDSMIKYVD LLWDWLCQVD 190 200 210 220 230 240 DIEDSTVAEF FSRLVFNYLF ISNISFAHES KDIFLERFIE KFHPKYEKID KNGYEIVFFE 250 260 270 280 290 300 DYSDLNFLQL LLITCQTKDK SYFLNLKNHY LDFSQAYKSE LEFLGQEYFN IVAPKQTNFL 310 QDMMSGFLGG SK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Analysis of a 22,956 bp region on the right arm of Saccharomyces cerevisiae chromosome XV." Madania A., Poch O., Tarassov I.A., Winsor B., Martin R.P. Yeast 12:1563-1573(1996) [PubMed: 8972579] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: S288c / FY1678.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XV." Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D. , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K. Nature 387:98-102(1997) [PubMed: 9169874] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[5]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]	"Comprehensive characterization of genes required for protein folding in the endoplasmic reticulum." Jonikas M.C., Collins S.R., Denic V., Oh E., Quan E.M., Schmid V., Weibezahn J., Schwappach B., Walter P., Weissman J.S., Schuldiner M. Science 323:1693-1697(2009) [PubMed: 19325107] [Abstract] Cited for: FUNCTION.
[8]	"Crystal structure of Get4-Get5 complex and its interactions with Sgt2, Get3, and Ydj1." Chang Y.W., Chuang Y.C., Ho Y.C., Cheng M.Y., Sun Y.J., Hsiao C.D., Wang C. J. Biol. Chem. 285:9962-9970(2010) [PubMed: 20106980] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.99 ANGSTROMS) IN COMPLEX WITH MDY2.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

U55021 Genomic DNA. Translation: AAB47411.1.
Z75072 Genomic DNA. Translation: CAA99370.1.
AY558036 Genomic DNA. Translation: AAS56362.1.
BK006948 Genomic DNA. Translation: DAA10938.1.

PIR

S67052.

RefSeq

NP_014807.1. NM_001183583.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2WPV	X-ray	1.99	A/C/E/G	1-312	[»]
3LKU	X-ray	2.80	A/C/E	11-300	[»]

ProteinModelPortal

Q12125.

SMR

Q12125. Positions 11-299.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-2005N.

IntAct

Q12125. 11 interactions.

MINT

MINT-396784.

STRING

Q12125.

Proteomic databases

PeptideAtlas

Q12125.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblFungi

YOR164C; YOR164C; YOR164C.

GeneID

854335.

KEGG

sce:YOR164C.

NMPDR

fig|4932.3.peg.5915.

Organism-specific databases

CYGD

YOR164c.

SGD

S000005690. GET4.

Phylogenomic databases

eggNOG

fuNOG07178.

GeneTree

EFGT00050000005203.

HOGENOM

HBG397316.

OMA

TGMNNWS.

OrthoDB

EOG4ZSDCM.

Gene expression databases

ArrayExpress

Q12125.

Genevestigator

Q12125.

GermOnline

YOR164C. Saccharomyces cerevisiae.

Family and domain databases

InterPro

IPR007317. UPF0363.
[Graphical view]

PANTHER

PTHR12875. DUF410. 1 hit.

Pfam

PF04190. DUF410. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

NextBio

976399.

Entry information

Entry name

GET4_YEAST

Accession

Primary (citable) accession number: Q12125
Secondary accession number(s): D6W2M2

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 21, 2006
Last sequence update:	November 1, 1996
Last modified:	December 14, 2011
This is version 85 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program