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Q12125 (GET4_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 105. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Golgi to ER traffic protein 4
Alternative name(s):
Guided entry of tail-anchored proteins 4
Gene names
Name:GET4
Ordered Locus Names:YOR164C
ORF Names:O3580
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length312 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

May play a role in insertion of tail-anchored proteins into the endoplasmic reticulum membrane. Ref.7

Subunit structure

Interacts with MDY2/GET5.

Subcellular location

Cytoplasm Ref.5.

Miscellaneous

Present with 5350 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the GET4 family.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

GET3Q121544EBI-36940,EBI-2989
MDY2Q122858EBI-36940,EBI-34904

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 312312Golgi to ER traffic protein 4
PRO_0000228107

Secondary structure

................................... 312
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q12125 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 98AFFDB4EA51B40F

FASTA31236,280
        10         20         30         40         50         60 
MVPAESNAVQ AKLAKTLQRF ENKIKAGDYY EAHQTLRTIA NRYVRSKSYE HAIELISQGA 

        70         80         90        100        110        120 
LSFLKAKQGG SGTDLIFYLL EVYDLAEVKV DDISVARLVR LIAELDPSEP NLKDVITGMN 

       130        140        150        160        170        180 
NWSIKFSEYK FGDPYLHNTI GSKLLEGDFV YEAERYFMLG THDSMIKYVD LLWDWLCQVD 

       190        200        210        220        230        240 
DIEDSTVAEF FSRLVFNYLF ISNISFAHES KDIFLERFIE KFHPKYEKID KNGYEIVFFE 

       250        260        270        280        290        300 
DYSDLNFLQL LLITCQTKDK SYFLNLKNHY LDFSQAYKSE LEFLGQEYFN IVAPKQTNFL 

       310 
QDMMSGFLGG SK 

« Hide

References

« Hide 'large scale' references
[1]"Analysis of a 22,956 bp region on the right arm of Saccharomyces cerevisiae chromosome XV."
Madania A., Poch O., Tarassov I.A., Winsor B., Martin R.P.
Yeast 12:1563-1573(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: S288c / FY1678.
[2]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XV."
Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D. expand/collapse author list , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.
Nature 387:98-102(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae."
Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. expand/collapse author list , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J.
Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[5]"Global analysis of protein localization in budding yeast."
Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K.
Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[6]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]"Comprehensive characterization of genes required for protein folding in the endoplasmic reticulum."
Jonikas M.C., Collins S.R., Denic V., Oh E., Quan E.M., Schmid V., Weibezahn J., Schwappach B., Walter P., Weissman J.S., Schuldiner M.
Science 323:1693-1697(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[8]"N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."
Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A., Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E., Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.
Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[9]"Crystal structure of Get4-Get5 complex and its interactions with Sgt2, Get3, and Ydj1."
Chang Y.W., Chuang Y.C., Ho Y.C., Cheng M.Y., Sun Y.J., Hsiao C.D., Wang C.
J. Biol. Chem. 285:9962-9970(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.99 ANGSTROMS) IN COMPLEX WITH MDY2.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U55021 Genomic DNA. Translation: AAB47411.1.
Z75072 Genomic DNA. Translation: CAA99370.1.
AY558036 Genomic DNA. Translation: AAS56362.1.
BK006948 Genomic DNA. Translation: DAA10938.1.
PIRS67052.
RefSeqNP_014807.3. NM_001183583.3.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2WPVX-ray1.99A/C/E/G1-312[»]
3LKUX-ray2.80A/C/E11-300[»]
4PWXX-ray5.40C/E11-290[»]
ProteinModelPortalQ12125.
SMRQ12125. Positions 11-299.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid34560. 75 interactions.
DIPDIP-2005N.
IntActQ12125. 9 interactions.
MINTMINT-396784.
STRING4932.YOR164C.

Proteomic databases

MaxQBQ12125.
PaxDbQ12125.
PeptideAtlasQ12125.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYOR164C; YOR164C; YOR164C.
GeneID854335.
KEGGsce:YOR164C.

Organism-specific databases

CYGDYOR164c.
SGDS000005690. GET4.

Phylogenomic databases

eggNOGNOG252493.
GeneTreeENSGT00390000015750.
OMARTITNRY.
OrthoDBEOG7F253S.

Enzyme and pathway databases

BioCycYEAST:G3O-33680-MONOMER.

Gene expression databases

GenevestigatorQ12125.

Family and domain databases

InterProIPR007317. UPF0363.
[Graphical view]
PANTHERPTHR12875. PTHR12875. 1 hit.
PfamPF04190. DUF410. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceQ12125.
NextBio976399.
PROQ12125.

Entry information

Entry nameGET4_YEAST
AccessionPrimary (citable) accession number: Q12125
Secondary accession number(s): D6W2M2
Entry history
Integrated into UniProtKB/Swiss-Prot: March 21, 2006
Last sequence update: November 1, 1996
Last modified: June 11, 2014
This is version 105 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XV

Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Uncharacterized protein families (UPF)

List of uncharacterized protein family (UPF) entries

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references