Reviewed, UniProtKB/Swiss-Prot Q12122 (HOSM_YEAST)

Names and origin

Protein names

Recommended name:
    Homocitrate synthase, mitochondrial
    EC=2.3.3.14

Gene names

Name:	LYS21
Ordered Locus Names:	YDL131W
ORF Names:	D2195

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	440 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	Acetyl-CoA + H2O + 2-oxoglutarate = (R)-2-hydroxybutane-1,2,4-tricarboxylate + CoA.
Pathway	Amino-acid biosynthesis; L-lysine biosynthesis via AAA pathway; L-alpha-aminoadipate from 2-oxoglutarate: step 1/4.
Subcellular location	Mitochondrion Potential.
Miscellaneous	Present with 21900 molecules/cell in log phase SD medium. Ref.3
Sequence similarities	Belongs to the alpha-IPM synthetase/homocitrate synthase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Lysine biosynthesis
Cellular component	Mitochondrion
Domain	Transit peptide
Molecular function	Transferase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	lysine biosynthetic process via aminoadipic acid Traceable author statement. Source: SGD
Cellular component	mitochondrion Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from direct assay. Source: SGD
Molecular function	homocitrate synthase activity Inferred from electronic annotation. Source: EC identical protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
itself		1	EBI-8508,EBI-8508
	P43582	1	EBI-8508,EBI-22766
ESS1	P22696	1	EBI-8508,EBI-6679
LYS20	P48570	1	EBI-8508,EBI-8502
PRP40	P33203	1	EBI-8508,EBI-701
SET2	P46995	1	EBI-8508,EBI-16985

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – ?		Mitochondrion Potential
	Chain	? – 440		Homocitrate synthase, mitochondrial		PRO_0000001051
Amino acid modifications
	Modified residue	217	1	Phosphoserine Ref.6
	Modified residue	332	1	Phosphoserine Ref.6
	Modified residue	333	1	Phosphothreonine Ref.6
	Modified residue	409	1	Phosphoserine Ref.6 Ref.4 Ref.5
	Modified residue	410	1	Phosphothreonine Ref.6 Ref.4 Ref.5

Sequences

	Sequence		Length	Mass (Da)
	Q12122-1 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: 6B01827D998FD9AD	FASTA	440	48,594
	10 20 30 40 50 60 MSENNEFQSV TESTTAPTTS NPYGPNPADY LSNVKNFQLI DSTLREGEQF ANAFFDTEKK 70 80 90 100 110 120 IEIARALDDF GVDYIELTSP VASEQSRKDC EAICKLGLKA KILTHIRCHM DDARVAVETG 130 140 150 160 170 180 VDGVDVVIGT SKFLRQYSHG KDMNYIAKSA VEVIEFVKSK GIEIRFSSED SFRSDLVDLL 190 200 210 220 230 240 NIYKTVDKIG VNRVGIADTV GCANPRQVYE LIRTLKSVVS CDIECHFHND TGCAIANAYT 250 260 270 280 290 300 ALEGGARLID VSVLGIGERN GITPLGGLMA RMIVAAPDYV RSKYKLHKIR DIENLVADAV 310 320 330 340 350 360 EVNIPFNNPI TGFCAFTHKA GIHAKAILAN PSTYEILDPH DFGMKRYIHF ANRLTGWNAI 370 380 390 400 410 420 KSRVDQLNLN LTDDQIKEVT AKIKKLGDVR PLNIDDVDSI IKDFHAELST PLLKPVNKGT 430 440 DDDNIDISNG HVSKKAKVTK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Analysis of a 26,756 bp segment from the left arm of yeast chromosome IV." Woelfl S., Haneman V., Saluz H.P. Yeast 12:1549-1554(1996) [PubMed: 8972577] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome IV." Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T. , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P. Nature 387:75-78(1997) [PubMed: 9169867] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[3]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[4]	"Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry." Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F. Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed: 17287358] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-409 AND THR-410, MASS SPECTROMETRY.
[5]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed: 17563356] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-409 AND THR-410, MASS SPECTROMETRY.
[6]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-217; SER-332; THR-333; SER-409 AND THR-410, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X96876 Genomic DNA. Translation: CAA65629.1. Z74179 Genomic DNA. Translation: CAA98700.1.
PIR	S67674.
RefSeq	NP_010151.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:4634N.
IntAct	Q12122. 16 interactions.
Proteomic databases
PeptideAtlas	Q12122.
PRIDE	Q12122.
Genome annotation databases
Ensembl	YDL131W. Saccharomyces cerevisiae. [Contig view]
GeneID	851425.
GenomeReviews	Gene locus YDL131W in contig Z71256_GR.
KEGG	sce:YDL131W.
NMPDR	fig|4932.3.peg.888.
Organism-specific databases
CYGD	YDL131w.
SGD	S000002289. LYS21.
Yeast-GFP	Search...
Phylogenomic databases
HOGENOM	Q12122.
OMA	Q12122. EITAMVE.
Enzyme and pathway databases
BRENDA	2.3.3.14. 250.
Gene expression databases
GermOnline	YDL131W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR002034. AIPM/Hcit_synth_CS. IPR013785. Aldolase_TIM. IPR011872. LysS_fun_arc. IPR000891. PYR_CT. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
PANTHER	PTHR10277:SF8. LysS_fun_arc. 1 hit.
Pfam	PF00682. HMGL-like. 1 hit. [Graphical view]
TIGRFAMs	TIGR02146. LysS_fung_arch. 1 hit.
PROSITE	PS00815. AIPM_HOMOCIT_SYNTH_1. 1 hit. PS00816. AIPM_HOMOCIT_SYNTH_2. 1 hit. PS50991. PYR_CT. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	968636.

Entry information

Entry name

HOSM_YEAST

Accession

Primary (citable) accession number: Q12122

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	June 16, 2009
This is version 83 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)