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Q12122

- HOSM_YEAST

UniProt

Q12122 - HOSM_YEAST

Protein

Homocitrate synthase, mitochondrial

Gene

LYS21

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 131 (01 Oct 2014)
      Sequence version 1 (01 Nov 1997)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    Acetyl-CoA + H2O + 2-oxoglutarate = (R)-2-hydroxybutane-1,2,4-tricarboxylate + CoA.

    Pathwayi

    GO - Molecular functioni

    1. homocitrate synthase activity Source: SGD

    GO - Biological processi

    1. lysine biosynthetic process via aminoadipic acid Source: SGD

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    Amino-acid biosynthesis, Lysine biosynthesis

    Enzyme and pathway databases

    BioCyciYEAST:YDL131W-MONOMER.
    SABIO-RKQ12122.
    UniPathwayiUPA00033; UER00028.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Homocitrate synthase, mitochondrial (EC:2.3.3.14)
    Gene namesi
    Name:LYS21
    Ordered Locus Names:YDL131W
    ORF Names:D2195
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome IV

    Organism-specific databases

    CYGDiYDL131w.
    SGDiS000002289. LYS21.

    Subcellular locationi

    GO - Cellular componenti

    1. mitochondrion Source: UniProtKB-SubCell
    2. nucleus Source: SGD

    Keywords - Cellular componenti

    Mitochondrion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini? – 440Homocitrate synthase, mitochondrialPRO_0000001051
    Transit peptidei1 – ?MitochondrionSequence Analysis

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei410 – 4101Phosphothreonine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiQ12122.
    PaxDbiQ12122.
    PeptideAtlasiQ12122.
    PRIDEiQ12122.

    Expressioni

    Gene expression databases

    GenevestigatoriQ12122.

    Interactioni

    Protein-protein interaction databases

    BioGridi31931. 41 interactions.
    DIPiDIP-4634N.
    IntActiQ12122. 15 interactions.
    MINTiMINT-537631.
    STRINGi4932.YDL131W.

    Structurei

    3D structure databases

    ProteinModelPortaliQ12122.
    SMRiQ12122. Positions 11-407.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiCOG0119.
    GeneTreeiENSGT00390000006101.
    HOGENOMiHOG000046858.
    KOiK01655.
    OMAiRDYVIDK.
    OrthoDBiEOG76HQ9T.

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    InterProiIPR002034. AIPM/Hcit_synth_CS.
    IPR013785. Aldolase_TIM.
    IPR011872. Homocitrate_synth_fun/arc.
    IPR000891. PYR_CT.
    [Graphical view]
    PfamiPF00682. HMGL-like. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR02146. LysS_fung_arch. 1 hit.
    PROSITEiPS00815. AIPM_HOMOCIT_SYNTH_1. 1 hit.
    PS00816. AIPM_HOMOCIT_SYNTH_2. 1 hit.
    PS50991. PYR_CT. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q12122-1 [UniParc]FASTAAdd to Basket

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    MSENNEFQSV TESTTAPTTS NPYGPNPADY LSNVKNFQLI DSTLREGEQF    50
    ANAFFDTEKK IEIARALDDF GVDYIELTSP VASEQSRKDC EAICKLGLKA 100
    KILTHIRCHM DDARVAVETG VDGVDVVIGT SKFLRQYSHG KDMNYIAKSA 150
    VEVIEFVKSK GIEIRFSSED SFRSDLVDLL NIYKTVDKIG VNRVGIADTV 200
    GCANPRQVYE LIRTLKSVVS CDIECHFHND TGCAIANAYT ALEGGARLID 250
    VSVLGIGERN GITPLGGLMA RMIVAAPDYV RSKYKLHKIR DIENLVADAV 300
    EVNIPFNNPI TGFCAFTHKA GIHAKAILAN PSTYEILDPH DFGMKRYIHF 350
    ANRLTGWNAI KSRVDQLNLN LTDDQIKEVT AKIKKLGDVR PLNIDDVDSI 400
    IKDFHAELST PLLKPVNKGT DDDNIDISNG HVSKKAKVTK 440
    Length:440
    Mass (Da):48,594
    Last modified:November 1, 1997 - v1
    Checksum:i6B01827D998FD9AD
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti199 – 1991T → A in AAT92960. (PubMed:17322287)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X96876 Genomic DNA. Translation: CAA65629.1.
    Z74179 Genomic DNA. Translation: CAA98700.1.
    AY692941 Genomic DNA. Translation: AAT92960.1.
    BK006938 Genomic DNA. Translation: DAA11728.1.
    PIRiS67674.
    RefSeqiNP_010151.1. NM_001180190.1.

    Genome annotation databases

    EnsemblFungiiYDL131W; YDL131W; YDL131W.
    GeneIDi851425.
    KEGGisce:YDL131W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X96876 Genomic DNA. Translation: CAA65629.1 .
    Z74179 Genomic DNA. Translation: CAA98700.1 .
    AY692941 Genomic DNA. Translation: AAT92960.1 .
    BK006938 Genomic DNA. Translation: DAA11728.1 .
    PIRi S67674.
    RefSeqi NP_010151.1. NM_001180190.1.

    3D structure databases

    ProteinModelPortali Q12122.
    SMRi Q12122. Positions 11-407.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 31931. 41 interactions.
    DIPi DIP-4634N.
    IntActi Q12122. 15 interactions.
    MINTi MINT-537631.
    STRINGi 4932.YDL131W.

    Proteomic databases

    MaxQBi Q12122.
    PaxDbi Q12122.
    PeptideAtlasi Q12122.
    PRIDEi Q12122.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YDL131W ; YDL131W ; YDL131W .
    GeneIDi 851425.
    KEGGi sce:YDL131W.

    Organism-specific databases

    CYGDi YDL131w.
    SGDi S000002289. LYS21.

    Phylogenomic databases

    eggNOGi COG0119.
    GeneTreei ENSGT00390000006101.
    HOGENOMi HOG000046858.
    KOi K01655.
    OMAi RDYVIDK.
    OrthoDBi EOG76HQ9T.

    Enzyme and pathway databases

    UniPathwayi UPA00033 ; UER00028 .
    BioCyci YEAST:YDL131W-MONOMER.
    SABIO-RK Q12122.

    Miscellaneous databases

    NextBioi 968636.

    Gene expression databases

    Genevestigatori Q12122.

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    InterProi IPR002034. AIPM/Hcit_synth_CS.
    IPR013785. Aldolase_TIM.
    IPR011872. Homocitrate_synth_fun/arc.
    IPR000891. PYR_CT.
    [Graphical view ]
    Pfami PF00682. HMGL-like. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR02146. LysS_fung_arch. 1 hit.
    PROSITEi PS00815. AIPM_HOMOCIT_SYNTH_1. 1 hit.
    PS00816. AIPM_HOMOCIT_SYNTH_2. 1 hit.
    PS50991. PYR_CT. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Analysis of a 26,756 bp segment from the left arm of yeast chromosome IV."
      Woelfl S., Haneman V., Saluz H.P.
      Yeast 12:1549-1554(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 96604 / S288c / FY1679.
    2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV."
      Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T.
      , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P.
      Nature 387:75-78(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    6. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
      Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
      J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: ADR376.
    7. "Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
      Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
      Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    8. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    9. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-410, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiHOSM_YEAST
    AccessioniPrimary (citable) accession number: Q12122
    Secondary accession number(s): D6VRL8, E9P8Z7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: November 1, 1997
    Last modified: October 1, 2014
    This is version 131 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 21900 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome IV
      Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

    External Data

    Dasty 3