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Q12122 (HOSM_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified December 14, 2011. Version 109. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Homocitrate synthase, mitochondrial
EC=2.3.3.14
Gene names
Name:LYS21
Ordered Locus Names:YDL131W
ORF Names:D2195
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length440 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

Acetyl-CoA + H2O + 2-oxoglutarate = (R)-2-hydroxybutane-1,2,4-tricarboxylate + CoA.

Pathway

Amino-acid biosynthesis; L-lysine biosynthesis via AAA pathway; L-alpha-aminoadipate from 2-oxoglutarate: step 1/4.

Subcellular location

Mitochondrion Potential.

Miscellaneous

Present with 21900 molecules/cell in log phase SD medium. Ref.5

Sequence similarities

Belongs to the alpha-IPM synthase/homocitrate synthase family.

Ontologies

Keywords
   Biological processAmino-acid biosynthesis
Lysine biosynthesis
   Cellular componentMitochondrion
   DomainTransit peptide
   Molecular functionTransferase
   PTMPhosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological processlysine biosynthetic process via aminoadipic acid

Inferred from mutant phenotype. Source: SGD

   Cellular componentmitochondrion

Inferred from electronic annotation. Source: UniProtKB-SubCell

nucleus

Inferred from direct assay. Source: SGD

   Molecular functionhomocitrate synthase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – ?Mitochondrion Potential
Chain? – 440Homocitrate synthase, mitochondrialPRO_0000001051

Amino acid modifications

Modified residue2171Phosphoserine Ref.8
Modified residue3321Phosphoserine Ref.8
Modified residue3331Phosphothreonine Ref.8
Modified residue4091Phosphoserine Ref.6 Ref.7 Ref.8
Modified residue4101Phosphothreonine Ref.6 Ref.7 Ref.8

Experimental info

Sequence conflict1991T → A in AAT92960. Ref.4

Sequences

Sequence LengthMass (Da)Tools
Q12122 [UniParc].

Last modified November 1, 1997. Version 1.
Checksum: 6B01827D998FD9AD

FASTA44048,594
        10         20         30         40         50         60 
MSENNEFQSV TESTTAPTTS NPYGPNPADY LSNVKNFQLI DSTLREGEQF ANAFFDTEKK 

        70         80         90        100        110        120 
IEIARALDDF GVDYIELTSP VASEQSRKDC EAICKLGLKA KILTHIRCHM DDARVAVETG 

       130        140        150        160        170        180 
VDGVDVVIGT SKFLRQYSHG KDMNYIAKSA VEVIEFVKSK GIEIRFSSED SFRSDLVDLL 

       190        200        210        220        230        240 
NIYKTVDKIG VNRVGIADTV GCANPRQVYE LIRTLKSVVS CDIECHFHND TGCAIANAYT 

       250        260        270        280        290        300 
ALEGGARLID VSVLGIGERN GITPLGGLMA RMIVAAPDYV RSKYKLHKIR DIENLVADAV 

       310        320        330        340        350        360 
EVNIPFNNPI TGFCAFTHKA GIHAKAILAN PSTYEILDPH DFGMKRYIHF ANRLTGWNAI 

       370        380        390        400        410        420 
KSRVDQLNLN LTDDQIKEVT AKIKKLGDVR PLNIDDVDSI IKDFHAELST PLLKPVNKGT 

       430        440 
DDDNIDISNG HVSKKAKVTK

« Hide

References

« Hide 'large scale' references
[1]"Analysis of a 26,756 bp segment from the left arm of yeast chromosome IV."
Woelfl S., Haneman V., Saluz H.P.
Yeast 12:1549-1554(1996) [PubMed: 8972577] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 96604 / S288c / FY1679.
[2]"The nucleotide sequence of Saccharomyces cerevisiae chromosome IV."
Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T. expand/collapse author list , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P.
Nature 387:75-78(1997) [PubMed: 9169867] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 96604 / S288c / FY1679.
[3]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae."
Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. expand/collapse author list , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J.
Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[5]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed: 14562106] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]"Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed: 17287358] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-409 AND THR-410, MASS SPECTROMETRY.
[7]"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases."
Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H.
Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed: 17563356] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-409 AND THR-410, MASS SPECTROMETRY.
[8]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-217; SER-332; THR-333; SER-409 AND THR-410, MASS SPECTROMETRY.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		X96876 Genomic DNA. Translation: CAA65629.1.
Z74179 Genomic DNA. Translation: CAA98700.1.
AY692941 Genomic DNA. Translation: AAT92960.1.
BK006938 Genomic DNA. Translation: DAA11728.1.
PIRS67674.
RefSeqNP_010151.1. NM_001180190.1.

3D structure databases

ProteinModelPortalQ12122.
SMRQ12122. Positions 11-407.
ModBaseSearch...

Protein-protein interaction databases

DIPDIP-4634N.
IntActQ12122. 16 interactions.
MINTMINT-537631.
STRINGQ12122.

Proteomic databases

PeptideAtlasQ12122.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYDL131W; YDL131W; YDL131W.
GeneID851425.
KEGGsce:YDL131W.
NMPDRfig|4932.3.peg.888.

Organism-specific databases

CYGDYDL131w.
SGDS000002289. LYS21.

Phylogenomic databases

eggNOGfuNOG04803.
HOGENOMHBG701974.
OMAETHFHND.
OrthoDBEOG4CRQ7N.

Gene expression databases

ArrayExpressQ12122.
GenevestigatorQ12122.
GermOnlineYDL131W. Saccharomyces cerevisiae.

Family and domain databases

InterProIPR002034. AIPM/Hcit_synth_CS.
IPR013785. Aldolase_TIM.
IPR011872. Homocitrate_synth_fun/arc.
IPR000891. PYR_CT.
[Graphical view]
Gene3DG3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
KOK01655.
PfamPF00682. HMGL-like. 1 hit.
[Graphical view]
TIGRFAMsTIGR02146. LysS_fung_arch. 1 hit.
PROSITEPS00815. AIPM_HOMOCIT_SYNTH_1. 1 hit.
PS00816. AIPM_HOMOCIT_SYNTH_2. 1 hit.
PS50991. PYR_CT. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio968636.

Entry information

Entry nameHOSM_YEAST
AccessionPrimary (citable) accession number: Q12122
Secondary accession number(s): D6VRL8, E9P8Z7
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: December 14, 2011
This is version 109 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents