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Protein

Homocitrate synthase, mitochondrial

Gene

LYS21

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Acetyl-CoA + H2O + 2-oxoglutarate = (R)-2-hydroxybutane-1,2,4-tricarboxylate + CoA.

Pathway: L-lysine biosynthesis via AAA pathway

This protein is involved in step 1 of the subpathway that synthesizes L-alpha-aminoadipate from 2-oxoglutarate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Homocitrate synthase, cytosolic isozyme (LYS20), Homocitrate synthase, mitochondrial (LYS21)
  2. Homocitrate dehydratase, mitochondrial (ACO2)
  3. Homoaconitase, mitochondrial (LYS4)
  4. Homoisocitrate dehydrogenase, mitochondrial (LYS12)
  5. Aromatic/aminoadipate aminotransferase 1 (ARO8)
This subpathway is part of the pathway L-lysine biosynthesis via AAA pathway, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-alpha-aminoadipate from 2-oxoglutarate, the pathway L-lysine biosynthesis via AAA pathway and in Amino-acid biosynthesis.

GO - Molecular functioni

  • homocitrate synthase activity Source: SGD

GO - Biological processi

  • lysine biosynthetic process via aminoadipic acid Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Amino-acid biosynthesis, Lysine biosynthesis

Enzyme and pathway databases

BioCyciYEAST:YDL131W-MONOMER.
BRENDAi2.3.3.14. 984.
SABIO-RKQ12122.
UniPathwayiUPA00033; UER00028.

Names & Taxonomyi

Protein namesi
Recommended name:
Homocitrate synthase, mitochondrial (EC:2.3.3.14)
Gene namesi
Name:LYS21
Ordered Locus Names:YDL131W
ORF Names:D2195
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311 Componenti: Chromosome IV

Organism-specific databases

CYGDiYDL131w.
EuPathDBiFungiDB:YDL131W.
SGDiS000002289. LYS21.

Subcellular locationi

GO - Cellular componenti

  • mitochondrion Source: UniProtKB-SubCell
  • nucleus Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini? – 440Homocitrate synthase, mitochondrialPRO_0000001051
Transit peptidei1 – ?MitochondrionSequence Analysis

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei399 – 3991PhosphoserineBy similarity
Modified residuei410 – 4101Phosphothreonine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ12122.
PaxDbiQ12122.
PeptideAtlasiQ12122.
PRIDEiQ12122.

Interactioni

Protein-protein interaction databases

BioGridi31931. 43 interactions.
DIPiDIP-4634N.
IntActiQ12122. 15 interactions.
MINTiMINT-537631.
STRINGi4932.YDL131W.

Structurei

3D structure databases

ProteinModelPortaliQ12122.
SMRiQ12122. Positions 11-407.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG0119.
GeneTreeiENSGT00390000006101.
HOGENOMiHOG000046858.
InParanoidiQ12122.
KOiK01655.
OMAiNDTGMAI.
OrthoDBiEOG76HQ9T.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
InterProiIPR002034. AIPM/Hcit_synth_CS.
IPR013785. Aldolase_TIM.
IPR011872. Homocitrate_synth_fun/arc.
IPR000891. PYR_CT.
[Graphical view]
PfamiPF00682. HMGL-like. 1 hit.
[Graphical view]
TIGRFAMsiTIGR02146. LysS_fung_arch. 1 hit.
PROSITEiPS00815. AIPM_HOMOCIT_SYNTH_1. 1 hit.
PS00816. AIPM_HOMOCIT_SYNTH_2. 1 hit.
PS50991. PYR_CT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q12122-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSENNEFQSV TESTTAPTTS NPYGPNPADY LSNVKNFQLI DSTLREGEQF
60 70 80 90 100
ANAFFDTEKK IEIARALDDF GVDYIELTSP VASEQSRKDC EAICKLGLKA
110 120 130 140 150
KILTHIRCHM DDARVAVETG VDGVDVVIGT SKFLRQYSHG KDMNYIAKSA
160 170 180 190 200
VEVIEFVKSK GIEIRFSSED SFRSDLVDLL NIYKTVDKIG VNRVGIADTV
210 220 230 240 250
GCANPRQVYE LIRTLKSVVS CDIECHFHND TGCAIANAYT ALEGGARLID
260 270 280 290 300
VSVLGIGERN GITPLGGLMA RMIVAAPDYV RSKYKLHKIR DIENLVADAV
310 320 330 340 350
EVNIPFNNPI TGFCAFTHKA GIHAKAILAN PSTYEILDPH DFGMKRYIHF
360 370 380 390 400
ANRLTGWNAI KSRVDQLNLN LTDDQIKEVT AKIKKLGDVR PLNIDDVDSI
410 420 430 440
IKDFHAELST PLLKPVNKGT DDDNIDISNG HVSKKAKVTK
Length:440
Mass (Da):48,594
Last modified:November 1, 1997 - v1
Checksum:i6B01827D998FD9AD
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti199 – 1991T → A in AAT92960 (PubMed:17322287).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X96876 Genomic DNA. Translation: CAA65629.1.
Z74179 Genomic DNA. Translation: CAA98700.1.
AY692941 Genomic DNA. Translation: AAT92960.1.
BK006938 Genomic DNA. Translation: DAA11728.1.
PIRiS67674.
RefSeqiNP_010151.1. NM_001180190.1.

Genome annotation databases

EnsemblFungiiYDL131W; YDL131W; YDL131W.
GeneIDi851425.
KEGGisce:YDL131W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X96876 Genomic DNA. Translation: CAA65629.1.
Z74179 Genomic DNA. Translation: CAA98700.1.
AY692941 Genomic DNA. Translation: AAT92960.1.
BK006938 Genomic DNA. Translation: DAA11728.1.
PIRiS67674.
RefSeqiNP_010151.1. NM_001180190.1.

3D structure databases

ProteinModelPortaliQ12122.
SMRiQ12122. Positions 11-407.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi31931. 43 interactions.
DIPiDIP-4634N.
IntActiQ12122. 15 interactions.
MINTiMINT-537631.
STRINGi4932.YDL131W.

Proteomic databases

MaxQBiQ12122.
PaxDbiQ12122.
PeptideAtlasiQ12122.
PRIDEiQ12122.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYDL131W; YDL131W; YDL131W.
GeneIDi851425.
KEGGisce:YDL131W.

Organism-specific databases

CYGDiYDL131w.
EuPathDBiFungiDB:YDL131W.
SGDiS000002289. LYS21.

Phylogenomic databases

eggNOGiCOG0119.
GeneTreeiENSGT00390000006101.
HOGENOMiHOG000046858.
InParanoidiQ12122.
KOiK01655.
OMAiNDTGMAI.
OrthoDBiEOG76HQ9T.

Enzyme and pathway databases

UniPathwayiUPA00033; UER00028.
BioCyciYEAST:YDL131W-MONOMER.
BRENDAi2.3.3.14. 984.
SABIO-RKQ12122.

Miscellaneous databases

NextBioi968636.
PROiQ12122.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
InterProiIPR002034. AIPM/Hcit_synth_CS.
IPR013785. Aldolase_TIM.
IPR011872. Homocitrate_synth_fun/arc.
IPR000891. PYR_CT.
[Graphical view]
PfamiPF00682. HMGL-like. 1 hit.
[Graphical view]
TIGRFAMsiTIGR02146. LysS_fung_arch. 1 hit.
PROSITEiPS00815. AIPM_HOMOCIT_SYNTH_1. 1 hit.
PS00816. AIPM_HOMOCIT_SYNTH_2. 1 hit.
PS50991. PYR_CT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Analysis of a 26,756 bp segment from the left arm of yeast chromosome IV."
    Woelfl S., Haneman V., Saluz H.P.
    Yeast 12:1549-1554(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 96604 / S288c / FY1679.
  2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV."
    Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T.
    , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P.
    Nature 387:75-78(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  6. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  7. "Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
    Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
    Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  8. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  9. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-410, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiHOSM_YEAST
AccessioniPrimary (citable) accession number: Q12122
Secondary accession number(s): D6VRL8, E9P8Z7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: June 24, 2015
This is version 138 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 21900 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.