Small glutamine-rich tetratricopeptide repeat-containing protein 2 - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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Q12118 (SGT2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 29, 2013. Version 102. History...

Clusters with 100%, 90%, 50% identity |

Documents (4) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Small glutamine-rich tetratricopeptide repeat-containing protein 2

Alternative name(s):
SGT/UBP
Viral protein U-binding protein

Gene names

Name:	SGT2
Ordered Locus Names:	YOR007C
ORF Names:	UNF346

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length	346 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Co-chaperone that binds to the molecular chaperone Hsp70 (SSA1 and SSA2). Regulates Hsp70 ATPase activity By similarity. Required for recovery from heat shock. Ref.5
Subunit structure	Interacts with HSC82, HSP104, MDY2, SSA1 AND SSA2. Ref.8
Subcellular location	Cytoplasm Ref.6.
Miscellaneous	Present with 9424 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the SGT family. Contains 4 TPR repeats.

Ontologies

Keywords
Cellular component	Cytoplasm
Domain	Repeat TPR repeat
Molecular function	Chaperone
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	posttranslational protein targeting to membrane Inferred from direct assay PubMed 20850366. Source: SGD response to heat Inferred from mutant phenotype Ref.5. Source: SGD
Cellular_component	TRC complex Inferred from direct assay PubMed 20850366. Source: SGD
Molecular_function	protein complex scaffold Inferred from mutant phenotype PubMed 20850366. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
MDY2	Q12285	9	EBI-31784,EBI-34904
YDJ1	P25491	2	EBI-31784,EBI-10420

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 346

346

Small glutamine-rich tetratricopeptide repeat-containing protein 2

PRO_0000106370

Regions

Repeat

102 – 135

TPR 1

Repeat

136 – 169

TPR 2

Repeat

170 – 203

TPR 3

Repeat

205 – 229

TPR 4

Amino acid modifications

Modified residue

Phosphoserine Ref.9

Modified residue

308

Phosphothreonine Ref.9

Modified residue

312

Phosphoserine Ref.9

Secondary structure

346

Helix Strand Turn

Details...

Sequences

Sequence

Length

Mass (Da)

Tools

Q12118 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 33539B74F742BD3C
Show »

FASTA

346

37,218

        10         20         30         40         50         60 
MSASKEEIAA LIVNYFSSIV EKKEISEDGA DSLNVAMDCI SEAFGFEREA VSGILGKSEF 

        70         80         90        100        110        120 
KGQHLADILN SASRVPESNK KDDAENVEIN IPEDDAETKA KAEDLKMQGN KAMANKDYEL 

       130        140        150        160        170        180 
AINKYTEAIK VLPTNAIYYA NRAAAHSSLK EYDQAVKDAE SAISIDPSYF RGYSRLGFAK 

       190        200        210        220        230        240 
YAQGKPEEAL EAYKKVLDIE GDNATEAMKR DYESAKKKVE QSLNLEKTVP EQSRDADVDA 

       250        260        270        280        290        300 
SQGASAGGLP DLGSLLGGGL GGLMNNPQLM QAAQKMMSNP GAMQNIQKMM QDPSIRQMAE 

       310        320        330        340 
GFASGGGTPN LSDLMNNPAL RNMAGNLFGG AGAQSTDETP DNENKQ

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The sequence of a 30 kb fragment on the left arm of chromosome XV from Saccharomyces cerevisiae reveals 15 open reading frames, five of which correspond to previously identified genes." Sterky F., Holmberg A., Pettersson B., Uhlen M. Yeast 12:1091-1095(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XV." Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D. Kleine K. Nature 387:98-102(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[4]	Bienvenut W.V., Peters C. Submitted (JUN-2005) to UniProtKB Cited for: PROTEIN SEQUENCE OF 131-142 AND 158-171, MASS SPECTROMETRY.
[5]	"Small glutamine-rich protein/viral protein U-binding protein is a novel cochaperone that affects heat shock protein 70 activity." Angeletti P.C., Walker D., Panganiban A.T. Cell Stress Chaperones 7:258-268(2002) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[6]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[7]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[8]	"SGT2 and MDY2 interact with molecular chaperone YDJ1 in Saccharomyces cerevisiae." Liou S.-T., Cheng M.-Y., Wang C. Cell Stress Chaperones 12:59-70(2007) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH HSC82; HSP104; MDY2; SSA1 AND SSA2.
[9]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-52; THR-308 AND SER-312, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

U43491 Genomic DNA. Translation: AAC49487.1.
Z74915 Genomic DNA. Translation: CAA99195.1.
BK006948 Genomic DNA. Translation: DAA10789.1.

PIR

S61991.

RefSeq

NP_014649.1. NM_001183426.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2LXB	NMR	-	A/B	2-72	[»]
2LXC	NMR	-	B/C	2-72	[»]
4ASV	NMR	-	A/B	1-78	[»]
4ASW	NMR	-	A/B	1-78	[»]

ProteinModelPortal

Q12118.

SMR

Q12118. Positions 2-240.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-1983N.

IntAct

Q12118. 12 interactions.

MINT

MINT-411674.

STRING

4932.YOR007C.

Proteomic databases

PaxDb

Q12118.

PeptideAtlas

Q12118.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblFungi

YOR007C; YOR007C; YOR007C.

GeneID

854168.

KEGG

sce:YOR007C.

Organism-specific databases

CYGD

YOR007c.

SGD

S000005533. SGT2.

Phylogenomic databases

eggNOG

COG0457.

GeneTree

ENSGT00650000093277.

HOGENOM

HOG000208193.

K16365.

OMA

RLGFAKY.

OrthoDB

EOG4HMNK6.

Enzyme and pathway databases

BioCyc

YEAST:G3O-33557-MONOMER.

Gene expression databases

Genevestigator

Q12118.

GermOnline

YOR007C. Saccharomyces cerevisiae.

Family and domain databases

Gene3D

1.25.40.10. 1 hit.

InterPro

IPR001440. TPR-1.
IPR013026. TPR-contain_dom.
IPR011990. TPR-like_helical.
IPR013105. TPR_2.
IPR019734. TPR_repeat.
[Graphical view]

Pfam

PF00515. TPR_1. 2 hits.
PF07719. TPR_2. 1 hit.
[Graphical view]

SMART

SM00028. TPR. 3 hits.
[Graphical view]

PROSITE

PS50005. TPR. 3 hits.
PS50293. TPR_REGION. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

NextBio

975956.

Entry information

Entry name

SGT2_YEAST

Accession

Primary (citable) accession number: Q12118
Secondary accession number(s): D6W273

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 22, 2005
Last sequence update:	November 1, 1996
Last modified:	May 29, 2013
This is version 102 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome XV

Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents