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Protein

Small glutamine-rich tetratricopeptide repeat-containing protein 2

Gene

SGT2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Co-chaperone that binds to the molecular chaperone Hsp70 (SSA1 and SSA2). Regulates Hsp70 ATPase activity (By similarity). Required for recovery from heat shock.By similarity1 Publication

GO - Molecular functioni

  • protein complex scaffold Source: SGD

GO - Biological processi

  • posttranslational protein targeting to membrane Source: SGD
  • response to heat Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Chaperone

Enzyme and pathway databases

BioCyciYEAST:G3O-33557-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Small glutamine-rich tetratricopeptide repeat-containing protein 2
Alternative name(s):
SGT/UBP
Viral protein U-binding protein
Gene namesi
Name:SGT2
Ordered Locus Names:YOR007C
ORF Names:UNF346
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311 Componenti: Chromosome XV

Organism-specific databases

CYGDiYOR007c.
EuPathDBiFungiDB:YOR007C.
SGDiS000005533. SGT2.

Subcellular locationi

GO - Cellular componenti

  • TRC complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 346346Small glutamine-rich tetratricopeptide repeat-containing protein 2PRO_0000106370Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei308 – 3081Phosphothreonine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ12118.
PaxDbiQ12118.
PeptideAtlasiQ12118.

Expressioni

Gene expression databases

GenevestigatoriQ12118.

Interactioni

Subunit structurei

Interacts with HSC82, HSP104, MDY2, SSA1 AND SSA2.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
MDY2Q122859EBI-31784,EBI-34904
YDJ1P254912EBI-31784,EBI-10420

Protein-protein interaction databases

BioGridi34410. 101 interactions.
DIPiDIP-1983N.
IntActiQ12118. 11 interactions.
MINTiMINT-411674.
STRINGi4932.YOR007C.

Structurei

Secondary structure

1
346
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi5 – 2117Combined sources
Helixi27 – 4418Combined sources
Helixi48 – 503Combined sources
Helixi51 – 566Combined sources
Helixi58 – 6811Combined sources
Helixi69 – 713Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2LXBNMR-A/B2-72[»]
2LXCNMR-B/C2-72[»]
3ZDMX-ray1.80A/B/D/E1-72[»]
4ASVNMR-A/B1-78[»]
4ASWNMR-A/B1-78[»]
ProteinModelPortaliQ12118.
SMRiQ12118. Positions 1-240.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati102 – 13534TPR 1Add
BLAST
Repeati136 – 16934TPR 2Add
BLAST
Repeati170 – 20334TPR 3Add
BLAST
Repeati205 – 22925TPR 4Add
BLAST

Sequence similaritiesi

Belongs to the SGT family.Curated
Contains 4 TPR repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, TPR repeat

Phylogenomic databases

eggNOGiCOG0457.
GeneTreeiENSGT00730000110724.
HOGENOMiHOG000208193.
InParanoidiQ12118.
KOiK16365.
OMAiQDPSIRQ.
OrthoDBiEOG77T1FP.

Family and domain databases

Gene3Di1.25.40.10. 1 hit.
InterProiIPR013026. TPR-contain_dom.
IPR011990. TPR-like_helical_dom.
IPR001440. TPR_1.
IPR013105. TPR_2.
IPR019734. TPR_repeat.
[Graphical view]
PfamiPF00515. TPR_1. 2 hits.
PF07719. TPR_2. 1 hit.
[Graphical view]
SMARTiSM00028. TPR. 3 hits.
[Graphical view]
PROSITEiPS50005. TPR. 3 hits.
PS50293. TPR_REGION. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q12118-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSASKEEIAA LIVNYFSSIV EKKEISEDGA DSLNVAMDCI SEAFGFEREA
60 70 80 90 100
VSGILGKSEF KGQHLADILN SASRVPESNK KDDAENVEIN IPEDDAETKA
110 120 130 140 150
KAEDLKMQGN KAMANKDYEL AINKYTEAIK VLPTNAIYYA NRAAAHSSLK
160 170 180 190 200
EYDQAVKDAE SAISIDPSYF RGYSRLGFAK YAQGKPEEAL EAYKKVLDIE
210 220 230 240 250
GDNATEAMKR DYESAKKKVE QSLNLEKTVP EQSRDADVDA SQGASAGGLP
260 270 280 290 300
DLGSLLGGGL GGLMNNPQLM QAAQKMMSNP GAMQNIQKMM QDPSIRQMAE
310 320 330 340
GFASGGGTPN LSDLMNNPAL RNMAGNLFGG AGAQSTDETP DNENKQ
Length:346
Mass (Da):37,218
Last modified:November 1, 1996 - v1
Checksum:i33539B74F742BD3C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U43491 Genomic DNA. Translation: AAC49487.1.
Z74915 Genomic DNA. Translation: CAA99195.1.
BK006948 Genomic DNA. Translation: DAA10789.1.
PIRiS61991.
RefSeqiNP_014649.1. NM_001183426.1.

Genome annotation databases

EnsemblFungiiYOR007C; YOR007C; YOR007C.
GeneIDi854168.
KEGGisce:YOR007C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U43491 Genomic DNA. Translation: AAC49487.1.
Z74915 Genomic DNA. Translation: CAA99195.1.
BK006948 Genomic DNA. Translation: DAA10789.1.
PIRiS61991.
RefSeqiNP_014649.1. NM_001183426.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2LXBNMR-A/B2-72[»]
2LXCNMR-B/C2-72[»]
3ZDMX-ray1.80A/B/D/E1-72[»]
4ASVNMR-A/B1-78[»]
4ASWNMR-A/B1-78[»]
ProteinModelPortaliQ12118.
SMRiQ12118. Positions 1-240.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34410. 101 interactions.
DIPiDIP-1983N.
IntActiQ12118. 11 interactions.
MINTiMINT-411674.
STRINGi4932.YOR007C.

Proteomic databases

MaxQBiQ12118.
PaxDbiQ12118.
PeptideAtlasiQ12118.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYOR007C; YOR007C; YOR007C.
GeneIDi854168.
KEGGisce:YOR007C.

Organism-specific databases

CYGDiYOR007c.
EuPathDBiFungiDB:YOR007C.
SGDiS000005533. SGT2.

Phylogenomic databases

eggNOGiCOG0457.
GeneTreeiENSGT00730000110724.
HOGENOMiHOG000208193.
InParanoidiQ12118.
KOiK16365.
OMAiQDPSIRQ.
OrthoDBiEOG77T1FP.

Enzyme and pathway databases

BioCyciYEAST:G3O-33557-MONOMER.

Miscellaneous databases

NextBioi975956.
PROiQ12118.

Gene expression databases

GenevestigatoriQ12118.

Family and domain databases

Gene3Di1.25.40.10. 1 hit.
InterProiIPR013026. TPR-contain_dom.
IPR011990. TPR-like_helical_dom.
IPR001440. TPR_1.
IPR013105. TPR_2.
IPR019734. TPR_repeat.
[Graphical view]
PfamiPF00515. TPR_1. 2 hits.
PF07719. TPR_2. 1 hit.
[Graphical view]
SMARTiSM00028. TPR. 3 hits.
[Graphical view]
PROSITEiPS50005. TPR. 3 hits.
PS50293. TPR_REGION. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The sequence of a 30 kb fragment on the left arm of chromosome XV from Saccharomyces cerevisiae reveals 15 open reading frames, five of which correspond to previously identified genes."
    Sterky F., Holmberg A., Pettersson B., Uhlen M.
    Yeast 12:1091-1095(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XV."
    Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D.
    , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.
    Nature 387:98-102(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Bienvenut W.V., Peters C.
    Submitted (JUN-2005) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 131-142 AND 158-171, IDENTIFICATION BY MASS SPECTROMETRY.
  5. "Small glutamine-rich protein/viral protein U-binding protein is a novel cochaperone that affects heat shock protein 70 activity."
    Angeletti P.C., Walker D., Panganiban A.T.
    Cell Stress Chaperones 7:258-268(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  6. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  8. "SGT2 and MDY2 interact with molecular chaperone YDJ1 in Saccharomyces cerevisiae."
    Liou S.-T., Cheng M.-Y., Wang C.
    Cell Stress Chaperones 12:59-70(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH HSC82; HSP104; MDY2; SSA1 AND SSA2.
  9. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-308, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  10. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiSGT2_YEAST
AccessioniPrimary (citable) accession number: Q12118
Secondary accession number(s): D6W273
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 22, 2005
Last sequence update: November 1, 1996
Last modified: May 27, 2015
This is version 119 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 9424 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XV
    Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.