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Q12117 (MRH1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 29, 2013. Version 108. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Protein MRH1
Alternative name(s):
Membrane protein related to HSP30
Gene names
Name:MRH1
Ordered Locus Names:YDR033W
ORF Names:YD9673.03
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length320 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Subcellular location

Cell membrane; Multi-pass membrane protein. Mitochondrion. Bud. Note: Localized primarily to the plasma membrane. Also found at the buds. Ref.3 Ref.5 Ref.6 Ref.9

Miscellaneous

Present with 182000 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the archaeal/bacterial/fungal opsin family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 320320Protein MRH1
PRO_0000196285

Regions

Topological domain1 – 3434Extracellular Potential
Transmembrane35 – 5521Helical; Potential
Topological domain56 – 627Cytoplasmic Potential
Transmembrane63 – 8321Helical; Potential
Topological domain84 – 11633Extracellular Potential
Transmembrane117 – 13721Helical; Potential
Topological domain138 – 1414Cytoplasmic Potential
Transmembrane142 – 16221Helical; Potential
Topological domain163 – 1675Extracellular Potential
Transmembrane168 – 18821Helical; Potential
Topological domain189 – 20416Cytoplasmic Potential
Transmembrane205 – 22521Helical; Potential
Topological domain226 – 23813Extracellular Potential
Transmembrane239 – 25921Helical; Potential
Topological domain260 – 32061Cytoplasmic Potential
Compositional bias300 – 31819Lys-rich

Amino acid modifications

Modified residue2891Phosphoserine Ref.4 Ref.8 Ref.10 Ref.12 Ref.13 Ref.14 Ref.15 Ref.16
Modified residue2951Phosphothreonine Ref.4 Ref.8 Ref.10 Ref.12 Ref.13 Ref.14 Ref.15 Ref.16
Modified residue2991Phosphoserine Ref.14

Sequences

Sequence LengthMass (Da)Tools
Q12117 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 4311F64EA6AA209F

FASTA32036,191
        10         20         30         40         50         60 
MSTFETLIKR GGNEAIKINP PTGADFHITS RGSDWFWTCF CCYLLFGLIL TFLMFRKPVN 

        70         80         90        100        110        120 
DRFFYLTGIA PNFFMCIAYF TMASNLGWIP VKAKYNHVQT STQKEHPGYR QIFYSRFVGW 

       130        140        150        160        170        180 
FLALPWPIIQ ICMLAGTPFW QMAFNVCITE FFTVCWLIAA CVHSTYKWGY YTIGLGAAIV 

       190        200        210        220        230        240 
VSISVMTTSY NLVKQRDNDI RLTFLVFFSI IMFLWIIAYP TCFGITDGGN VLQPDSAGIF 

       250        260        270        280        290        300 
YGIIDLILMC FIPTLLVPIA NHFGADKLGY HFGPSDAEAV MAPKAPVASP RPAATPNLSK 

       310        320 
DKKKKSKKSK KSKKSKKSEE

« Hide

References

« Hide 'large scale' references
[1]"The nucleotide sequence of Saccharomyces cerevisiae chromosome IV."
Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T. expand/collapse author list , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P.
Nature 387:75-78(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204511 / S288c / AB972.
[2]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[3]"Expression and subcellular localization of a membrane protein related to Hsp30p in Saccharomyces cerevisiae."
Wu K., Dawe J.H., Aris J.P.
Biochim. Biophys. Acta 1463:477-482(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION.
[4]"Phosphoproteome analysis by mass spectrometry and its application to Saccharomyces cerevisiae."
Ficarro S.B., McCleland M.L., Stukenberg P.T., Burke D.J., Ross M.M., Shabanowitz J., Hunt D.F., White F.M.
Nat. Biotechnol. 20:301-305(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-289 AND THR-295, MASS SPECTROMETRY.
Strain: 2124.
[5]"Subproteomics: identification of plasma membrane proteins from the yeast Saccharomyces cerevisiae."
Navarre C., Degand H., Bennett K.L., Crawford J.S., Moertz E., Boutry M.
Proteomics 2:1706-1714(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION.
[6]"Global analysis of protein localization in budding yeast."
Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K.
Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[7]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[8]"A proteomics approach to understanding protein ubiquitination."
Peng J., Schwartz D., Elias J.E., Thoreen C.C., Cheng D., Marsischky G., Roelofs J., Finley D., Gygi S.P.
Nat. Biotechnol. 21:921-926(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-289 AND THR-295, MASS SPECTROMETRY.
Strain: SUB592.
[9]"The proteome of Saccharomyces cerevisiae mitochondria."
Sickmann A., Reinders J., Wagner Y., Joppich C., Zahedi R.P., Meyer H.E., Schoenfisch B., Perschil I., Chacinska A., Guiard B., Rehling P., Pfanner N., Meisinger C.
Proc. Natl. Acad. Sci. U.S.A. 100:13207-13212(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.
Strain: ATCC 76625 / YPH499.
[10]"Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway."
Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N.
Mol. Cell. Proteomics 4:310-327(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-289 AND THR-295, MASS SPECTROMETRY.
Strain: YAL6B.
[11]"A global topology map of the Saccharomyces cerevisiae membrane proteome."
Kim H., Melen K., Oesterberg M., von Heijne G.
Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
Strain: ATCC 208353 / W303-1A.
[12]"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-289 AND THR-295, MASS SPECTROMETRY.
Strain: ADR376.
[13]"Profiling phosphoproteins of yeast mitochondria reveals a role of phosphorylation in assembly of the ATP synthase."
Reinders J., Wagner K., Zahedi R.P., Stojanovski D., Eyrich B., van der Laan M., Rehling P., Sickmann A., Pfanner N., Meisinger C.
Mol. Cell. Proteomics 6:1896-1906(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-289 AND THR-295, MASS SPECTROMETRY.
Strain: ATCC 76625 / YPH499.
[14]"Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-289; THR-295 AND SER-299, MASS SPECTROMETRY.
[15]"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases."
Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H.
Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-289 AND THR-295, MASS SPECTROMETRY.
[16]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-289 AND THR-295, MASS SPECTROMETRY.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		Z74329 Genomic DNA. Translation: CAA98855.1.
Z68196 Genomic DNA. Translation: CAA92370.1.
BK006938 Genomic DNA. Translation: DAA11877.1.
PIRS61586.
RefSeqNP_010316.1. NM_001180341.1.

3D structure databases

ProteinModelPortalQ12117.
ModBaseSearch...

Protein-protein interaction databases

MINTMINT-2732197.
STRING4932.YDR033W.

Proteomic databases

PaxDbQ12117.
PeptideAtlasQ12117.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYDR033W; YDR033W; YDR033W.
GeneID851597.
KEGGsce:YDR033W.

Organism-specific databases

CYGDYDR033w.
SGDS000002440. MRH1.

Phylogenomic databases

eggNOGCOG5524.
GeneTreeENSGT00390000004481.
HOGENOMHOG000248243.
OMAAESIHEN.
OrthoDBEOG4HX897.

Enzyme and pathway databases

BioCycYEAST:G3O-29648-MONOMER.

Gene expression databases

GenevestigatorQ12117.
GermOnlineYDR033W. Saccharomyces cerevisiae.

Family and domain databases

InterProIPR001425. Rhodopsin_arc/bac/fun.
[Graphical view]
PfamPF01036. Bac_rhodopsin. 1 hit.
[Graphical view]
SMARTSM01021. Bac_rhodopsin. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio969087.

Entry information

Entry nameMRH1_YEAST
AccessionPrimary (citable) accession number: Q12117
Secondary accession number(s): D6VS17
Entry history
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: November 1, 1996
Last modified: May 29, 2013
This is version 108 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome IV

Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

7-transmembrane G-linked receptors

List of 7-transmembrane G-linked receptor entries

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents