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Protein

Chitin biosynthesis protein CHS5

Gene

CHS5

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the CHS5/6 complex which mediates export of specific cargo proteins, including chitin synthase CHS3. Also involved in targeting FUS1 to sites of polarized growth.8 Publications

GO - Biological processi

  • ascospore wall assembly Source: SGD
  • cellular bud site selection Source: SGD
  • cell wall chitin catabolic process Source: SGD
  • conjugation with cellular fusion Source: SGD
  • Golgi to plasma membrane transport Source: SGD
  • protein transport Source: UniProtKB-KW
  • regulation of transcription, DNA-templated Source: SGD
Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Enzyme and pathway databases

BioCyciYEAST:YLR330W-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Chitin biosynthesis protein CHS5
Alternative name(s):
Protein CAL3
Gene namesi
Name:CHS5
Synonyms:CAL3
Ordered Locus Names:YLR330W
ORF Names:L8543.18
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XII

Organism-specific databases

EuPathDBiFungiDB:YLR330W.
SGDiS000004322. CHS5.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: SGD
  • exomer complex Source: SGD
  • membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000896611 – 671Chitin biosynthesis protein CHS5Add BLAST671

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei305PhosphothreonineCombined sources1
Modified residuei338PhosphoserineCombined sources1
Modified residuei362PhosphoserineCombined sources1
Modified residuei365PhosphoserineCombined sources1
Modified residuei383PhosphoserineCombined sources1
Modified residuei384PhosphoserineCombined sources1
Modified residuei573PhosphoserineCombined sources1
Modified residuei579PhosphoserineCombined sources1
Cross-linki584Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources
Modified residuei590PhosphoserineCombined sources1

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiQ12114.
PRIDEiQ12114.

PTM databases

iPTMnetiQ12114.

Interactioni

Subunit structurei

Component of the CHS5/6 complex composed of the 4 CHAPS proteins BCH1, BCH2, BUD7, and CHS6 as well as at least CHS5 and GTP-bound ARF1. The complex interacts with the cargo protein CHS3.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
ARF1P110765EBI-4640,EBI-2816
BCH1Q0502913EBI-4640,EBI-27508
BCH2P361227EBI-4640,EBI-26374
BUD7Q087549EBI-4640,EBI-32770
CHS6P4095516EBI-4640,EBI-4649

Protein-protein interaction databases

BioGridi31594. 254 interactors.
DIPiDIP-8073N.
IntActiQ12114. 41 interactors.
MINTiMINT-1174352.

Structurei

Secondary structure

1671
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi4 – 13Combined sources10
Beta strandi19 – 21Combined sources3
Beta strandi27 – 31Combined sources5
Turni32 – 34Combined sources3
Beta strandi44 – 53Combined sources10
Helixi57 – 74Combined sources18
Beta strandi84 – 89Combined sources6
Beta strandi94 – 98Combined sources5
Beta strandi108 – 117Combined sources10
Turni126 – 128Combined sources3
Beta strandi131 – 134Combined sources4
Beta strandi142 – 151Combined sources10
Beta strandi154 – 164Combined sources11
Beta strandi176 – 178Combined sources3
Helixi183 – 185Combined sources3
Helixi189 – 198Combined sources10
Beta strandi205 – 207Combined sources3
Beta strandi214 – 216Combined sources3
Helixi227 – 234Combined sources8
Helixi243 – 250Combined sources8
Helixi258 – 260Combined sources3
Helixi266 – 272Combined sources7
Helixi279 – 284Combined sources6

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4IN3X-ray2.94A/C1-77[»]
4WJWX-ray2.59A1-77[»]
4YG8X-ray2.75A50-299[»]
ProteinModelPortaliQ12114.
SMRiQ12114.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini78 – 168Fibronectin type-IIIPROSITE-ProRule annotationAdd BLAST91
Domaini166 – 262BRCTPROSITE-ProRule annotationAdd BLAST97

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi279 – 635Glu-richAdd BLAST357
Compositional biasi645 – 671Lys-richAdd BLAST27

Sequence similaritiesi

Belongs to the CHS5 family.Curated
Contains 1 BRCT domain.PROSITE-ProRule annotation
Contains 1 fibronectin type-III domain.PROSITE-ProRule annotation

Phylogenomic databases

GeneTreeiENSGT00730000113735.
HOGENOMiHOG000093664.
InParanoidiQ12114.
KOiK19877.
OMAiESAGENN.
OrthoDBiEOG092C3V5H.

Family and domain databases

CDDicd00027. BRCT. 1 hit.
cd13945. Chs5_N. 1 hit.
cd00063. FN3. 1 hit.
Gene3Di2.60.40.10. 1 hit.
3.40.50.10190. 1 hit.
InterProiIPR001357. BRCT_dom.
IPR031673. Chs5_N.
IPR031669. Fn3_2.
IPR003961. FN3_dom.
IPR013783. Ig-like_fold.
[Graphical view]
PfamiPF00533. BRCT. 1 hit.
PF16892. CHS5_N. 1 hit.
PF16893. fn3_2. 1 hit.
[Graphical view]
SMARTiSM00292. BRCT. 1 hit.
SM00060. FN3. 1 hit.
[Graphical view]
SUPFAMiSSF49265. SSF49265. 1 hit.
SSF52113. SSF52113. 1 hit.
PROSITEiPS50172. BRCT. 1 hit.
PS50853. FN3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q12114-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSVDVLLTV GKLDASLALL TTQDHHVIEF PTVLLPENVK AGSIIKMQVS
60 70 80 90 100
QNLEEEKKQR NHFKSIQAKI LEKYGTHKPE SPVLKIVNVT QTSCVLAWDP
110 120 130 140 150
LKLGSAKLKS LILYRKGIRS MVIPNPFKVT TTKISGLSVD TPYEFQLKLI
160 170 180 190 200
TTSGTLWSEK VILRTHKMTD MSGITVCLGP LDPLKEISDL QISQCLSHIG
210 220 230 240 250
ARPLQRHVAI DTTHFVCNDL DNEESNEELI RAKHNNIPIV RPEWVRACEV
260 270 280 290 300
EKRIVGVRGF YLDADQSILK NYTFPPVNEE ELSYSKENEP VAEVADENKM
310 320 330 340 350
PEDTTDVEQV ASPNDNESNP SEAKEQGEKS GHETAPVSPV EDPLHASTAL
360 370 380 390 400
ENETTIETVN PSVRSLKSEP VGTPNIEENK ADSSAEAVVE EPNEAVAESS
410 420 430 440 450
PNEEATGQKS EDTDTHSNEQ ADNGFVQTEE VAENNITTES AGENNEPADD
460 470 480 490 500
AAMEFGRPEA EIETPEVNES IEDANEPAED SNEPVEDSNK PVKDSNKPVE
510 520 530 540 550
DSNKPVEDSN KPVEDSNKPV EDANEPVEDT SEPVEDAGEP VQETNEFTTD
560 570 580 590 600
IASPRHQEED IELEAEPKDA TESVAVEPSN EDVKPEEKGS EAEDDINNVS
610 620 630 640 650
KEAASGESTT HQKTEASASL ESSAVTEEQE TTEAEVNTDD VLSTKEAKKN
660 670
TGNSNSNKKK NKKNKKKGKK K
Length:671
Mass (Da):73,639
Last modified:November 1, 1997 - v1
Checksum:iFA92741B862814C2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z49198 Genomic DNA. Translation: CAA89059.1.
U20618 Genomic DNA. Translation: AAB64526.1.
BK006945 Genomic DNA. Translation: DAA09638.1.
PIRiS53407.
RefSeqiNP_013434.1. NM_001182219.1.

Genome annotation databases

EnsemblFungiiYLR330W; YLR330W; YLR330W.
GeneIDi851041.
KEGGisce:YLR330W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z49198 Genomic DNA. Translation: CAA89059.1.
U20618 Genomic DNA. Translation: AAB64526.1.
BK006945 Genomic DNA. Translation: DAA09638.1.
PIRiS53407.
RefSeqiNP_013434.1. NM_001182219.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4IN3X-ray2.94A/C1-77[»]
4WJWX-ray2.59A1-77[»]
4YG8X-ray2.75A50-299[»]
ProteinModelPortaliQ12114.
SMRiQ12114.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi31594. 254 interactors.
DIPiDIP-8073N.
IntActiQ12114. 41 interactors.
MINTiMINT-1174352.

PTM databases

iPTMnetiQ12114.

Proteomic databases

MaxQBiQ12114.
PRIDEiQ12114.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYLR330W; YLR330W; YLR330W.
GeneIDi851041.
KEGGisce:YLR330W.

Organism-specific databases

EuPathDBiFungiDB:YLR330W.
SGDiS000004322. CHS5.

Phylogenomic databases

GeneTreeiENSGT00730000113735.
HOGENOMiHOG000093664.
InParanoidiQ12114.
KOiK19877.
OMAiESAGENN.
OrthoDBiEOG092C3V5H.

Enzyme and pathway databases

BioCyciYEAST:YLR330W-MONOMER.

Miscellaneous databases

PROiQ12114.

Family and domain databases

CDDicd00027. BRCT. 1 hit.
cd13945. Chs5_N. 1 hit.
cd00063. FN3. 1 hit.
Gene3Di2.60.40.10. 1 hit.
3.40.50.10190. 1 hit.
InterProiIPR001357. BRCT_dom.
IPR031673. Chs5_N.
IPR031669. Fn3_2.
IPR003961. FN3_dom.
IPR013783. Ig-like_fold.
[Graphical view]
PfamiPF00533. BRCT. 1 hit.
PF16892. CHS5_N. 1 hit.
PF16893. fn3_2. 1 hit.
[Graphical view]
SMARTiSM00292. BRCT. 1 hit.
SM00060. FN3. 1 hit.
[Graphical view]
SUPFAMiSSF49265. SSF49265. 1 hit.
SSF52113. SSF52113. 1 hit.
PROSITEiPS50172. BRCT. 1 hit.
PS50853. FN3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCHS5_YEAST
AccessioniPrimary (citable) accession number: Q12114
Secondary accession number(s): D6VYX2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: November 30, 2016
This is version 147 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 172 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XII
    Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.