Reviewed, UniProtKB/Swiss-Prot Q12114 (CHS5_YEAST)

Names and origin

Protein names

Recommended name:
    Chitin biosynthesis protein CHS5
Alternative name(s):
    Protein CAL3

Gene names

Name:	CHS5
Synonyms:	CAL3
Ordered Locus Names:	YLR330W
ORF Names:	L8543.18

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	671 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Component of the CHS5/6 complex which mediates export of specific cargo proteins, including chitin synthase CHS3. Also involved in targeting FUS1 to sites of polarized growth. Ref.1 Ref.3 Ref.6 Ref.9 Ref.10 Ref.11 Ref.12 Ref.13
Subunit structure	Component of the CHS5/6 complex composed of the 4 CHAPS proteins BCH1, BCH2, BUD7, and CHS6 as well as at least CHS5 and GTP-bound ARF1. The complex interacts with the cargo protein CHS3. Ref.10 Ref.12 Ref.13
Subcellular location	Golgi apparatus › trans-Golgi network membrane; Peripheral membrane protein. Note: Trans-Golgi network location requires interaction with myristoylated GTP-bound ARF1 for the recruitement to the membranes. Ref.10 Ref.12 Ref.5 Ref.7
Miscellaneous	Present with 172 molecules/cell in log phase SD medium. Ref.8
Sequence similarities	Belongs to the CHS5 family. Contains 1 BRCT domain. Contains 1 fibronectin type-III domain.

Ontologies

Keywords
Biological process	Fusion protein Protein transport Transport
Cellular component	Golgi apparatus Membrane
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	Golgi to plasma membrane transport Ref.10 Inferred from mutant phenotype. Source: SGD ascospore wall assembly Ref.1 Inferred from mutant phenotype. Source: SGD cell wall chitin catabolic process Ref.1 Inferred from mutant phenotype. Source: SGD cellular bud site selection Ref.10 Inferred from mutant phenotype. Source: SGD conjugation with cellular fusion Ref.1 Inferred from mutant phenotype. Source: SGD protein transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	exomer complex Ref.12 Inferred from direct assay. Source: SGD extrinsic to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell mating projection tip Inferred from direct assay. Source: SGD trans-Golgi network membrane Ref.12 Inferred from direct assay. Source: SGD
Molecular function	identical protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
itself		1	EBI-4640,EBI-4640
	Q03687	1	EBI-4640,EBI-28024
APL6	P46682	1	EBI-4640,EBI-2213
DNF1	P32660	1	EBI-4640,EBI-3121
FLC2	P39719	1	EBI-4640,EBI-20703
KEX1	P09620	1	EBI-4640,EBI-9653
LAA1	P39526	1	EBI-4640,EBI-26235
SEC7	P11075	1	EBI-4640,EBI-16882
SMI1	P32566	1	EBI-4640,EBI-17452
VMA4	P22203	1	EBI-4640,EBI-20268

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 671	671	Chitin biosynthesis protein CHS5		PRO_0000089661
Regions
	Domain	79 – 165	87	Fibronectin type-III
	Domain	166 – 262	97	BRCT
	Compositional bias	279 – 635	357	Glu-rich
	Compositional bias	645 – 671	27	Lys-rich
Amino acid modifications
	Modified residue	334	1	Phosphothreonine Ref.15
	Modified residue	338	1	Phosphoserine Ref.15 Ref.16
	Modified residue	347	1	Phosphoserine Ref.15
	Modified residue	362	1	Phosphoserine Ref.16
	Modified residue	383	1	Phosphoserine Ref.16
	Modified residue	553	1	Phosphoserine Ref.16
	Modified residue	573	1	Phosphoserine Ref.15 Ref.16
	Modified residue	579	1	Phosphoserine Ref.16
	Modified residue	590	1	Phosphoserine Ref.15 Ref.16 Ref.14

Sequences

	Sequence		Length	Mass (Da)
	Q12114-1 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: FA92741B862814C2	FASTA	671	73,639
	10 20 30 40 50 60 MSSVDVLLTV GKLDASLALL TTQDHHVIEF PTVLLPENVK AGSIIKMQVS QNLEEEKKQR 70 80 90 100 110 120 NHFKSIQAKI LEKYGTHKPE SPVLKIVNVT QTSCVLAWDP LKLGSAKLKS LILYRKGIRS 130 140 150 160 170 180 MVIPNPFKVT TTKISGLSVD TPYEFQLKLI TTSGTLWSEK VILRTHKMTD MSGITVCLGP 190 200 210 220 230 240 LDPLKEISDL QISQCLSHIG ARPLQRHVAI DTTHFVCNDL DNEESNEELI RAKHNNIPIV 250 260 270 280 290 300 RPEWVRACEV EKRIVGVRGF YLDADQSILK NYTFPPVNEE ELSYSKENEP VAEVADENKM 310 320 330 340 350 360 PEDTTDVEQV ASPNDNESNP SEAKEQGEKS GHETAPVSPV EDPLHASTAL ENETTIETVN 370 380 390 400 410 420 PSVRSLKSEP VGTPNIEENK ADSSAEAVVE EPNEAVAESS PNEEATGQKS EDTDTHSNEQ 430 440 450 460 470 480 ADNGFVQTEE VAENNITTES AGENNEPADD AAMEFGRPEA EIETPEVNES IEDANEPAED 490 500 510 520 530 540 SNEPVEDSNK PVKDSNKPVE DSNKPVEDSN KPVEDSNKPV EDANEPVEDT SEPVEDAGEP 550 560 570 580 590 600 VQETNEFTTD IASPRHQEED IELEAEPKDA TESVAVEPSN EDVKPEEKGS EAEDDINNVS 610 620 630 640 650 660 KEAASGESTT HQKTEASASL ESSAVTEEQE TTEAEVNTDD VLSTKEAKKN TGNSNSNKKK 670 NKKNKKKGKK K

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"CHS5, a gene involved in chitin synthesis and mating in Saccharomyces cerevisiae." Santos B., Duran A., Valdivieso M.H. Mol. Cell. Biol. 17:2485-2496(1997) [PubMed: 9111317] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION. Strain: S288c / GRF88.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XII." Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H. , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D. Nature 387:87-90(1997) [PubMed: 9169871] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	"Chitin synthase 3 from yeast has zymogenic properties that depend on both the CAL1 and the CAL3 genes." Choi W.-J., Sburlati A., Cabib E. Proc. Natl. Acad. Sci. U.S.A. 91:4727-4730(1994) [PubMed: 8197125] [Abstract] Cited for: FUNCTION.
[4]	"Fibronectin type III domains in yeast detected by a hidden Markov model." Bateman A., Chothia C. Curr. Biol. 6:1544-1546(1996) [PubMed: 8994808] [Abstract] Cited for: DOMAIN FIBRONECTIN TYPE-III.
[5]	"Targeting of chitin synthase 3 to polarized growth sites in yeast requires Chs5p and Myo2p." Santos B., Snyder M. J. Cell Biol. 136:95-110(1997) [PubMed: 9008706] [Abstract] Cited for: SUBCELLULAR LOCATION.
[6]	"Specific protein targeting during cell differentiation: polarized localization of Fus1p during mating depends on Chs5p in Saccharomyces cerevisiae." Santos B., Snyder M. Eukaryot. Cell 2:821-825(2003) [PubMed: 12912901] [Abstract] Cited for: FUNCTION.
[7]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[8]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[9]	"An interactional network of genes involved in chitin synthesis in Saccharomyces cerevisiae." Lesage G., Shapiro J., Specht C.A., Sdicu A.-M., Menard P., Hussein S., Tong A.H.Y., Boone C., Bussey H. BMC Genet. 6:8-8(2005) [PubMed: 15715908] [Abstract] Cited for: FUNCTION.
[10]	"Arf1p, Chs5p and the ChAPs are required for export of specialized cargo from the Golgi." Trautwein M., Schindler C., Gauss R., Dengjel J., Hartmann E., Spang A. EMBO J. 25:943-954(2006) [PubMed: 16498409] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH ARF1; BCH1; BCH2; BUD7; CHS3 AND CHS6, DOMAIN.
[11]	"Palmitoylation by the DHHC protein Pfa4 regulates the ER exit of Chs3." Lam K.K.Y., Davey M., Sun B., Roth A.F., Davis N.G., Conibear E. J. Cell Biol. 174:19-25(2006) [PubMed: 16818716] [Abstract] Cited for: FUNCTION.
[12]	"Exomer: a coat complex for transport of select membrane proteins from the trans-Golgi network to the plasma membrane in yeast." Wang C.-W., Hamamoto S., Orci L., Schekman R. J. Cell Biol. 174:973-983(2006) [PubMed: 17000877] [Abstract] Cited for: FUNCTION, IDENTIFICATION IN THE CHS5/6 COMPLEX, INTERACTION WITH ARF1, SUBCELLULAR LOCATION.
[13]	"Chs5/6 complex: a multiprotein complex that interacts with and conveys chitin synthase III from the trans-Golgi network to the cell surface." Sanchatjate S., Schekman R. Mol. Biol. Cell 17:4157-4166(2006) [PubMed: 16855022] [Abstract] Cited for: FUNCTION, IDENTIFICATION IN THE CHS5/6 COMPLEX, INTERACTION WITH CHS3.
[14]	"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed: 17330950] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-590, MASS SPECTROMETRY.
[15]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed: 17563356] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-334; SER-338; SER-347; SER-573 AND SER-590, MASS SPECTROMETRY.
[16]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-338; SER-362; SER-383; SER-553; SER-573; SER-579 AND SER-590, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z49198 Genomic DNA. Translation: CAA89059.1. U20618 Genomic DNA. Translation: AAB64526.1.
PIR	S53407.
RefSeq	NP_013434.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	Q12114. 46 interactions.
STRING	Q12114.
Proteomic databases
PeptideAtlas	Q12114.
PRIDE	Q12114.
Genome annotation databases
Ensembl	YLR330W; YLR330W; YLR330W; Saccharomyces cerevisiae. [Genome view]
GeneID	851041.
KEGG	sce:YLR330W.
NMPDR	fig|4932.3.peg.4454.
Organism-specific databases
CYGD	YLR330w.
SGD	S000004322. CHS5.
Phylogenomic databases
HOGENOM	Q12114.
OMA	PVEDSNK
OrthoDB	EOG9H47M3
Gene expression databases
ArrayExpress	Q12114.
Genevestigator	Q12114.
GermOnline	YLR330W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR001357. BRCT. IPR008957. Fibronectin_typ-III-like_fold. IPR003961. FN_III. [Graphical view]
Pfam	PF00533. BRCT. 1 hit. PF00041. fn3. 1 hit. [Graphical view]
SMART	SM00292. BRCT. 1 hit. SM00060. FN3. 1 hit. [Graphical view]
PROSITE	PS50172. BRCT. 1 hit. PS50853. FN3. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	967645.

Entry information

Entry name

CHS5_YEAST

Accession

Primary (citable) accession number: Q12114

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	November 24, 2009
This is version 81 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)