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Q12109

- SYWC_YEAST

UniProt

Q12109 - SYWC_YEAST

Protein

Tryptophan--tRNA ligase, cytoplasmic

Gene

WRS1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 114 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    ATP + L-tryptophan + tRNA(Trp) = AMP + diphosphate + L-tryptophyl-tRNA(Trp).

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. tryptophan-tRNA ligase activity Source: SGD

    GO - Biological processi

    1. tryptophanyl-tRNA aminoacylation Source: SGD

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciYEAST:G3O-33496-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Tryptophan--tRNA ligase, cytoplasmic (EC:6.1.1.2)
    Alternative name(s):
    Tryptophanyl-tRNA synthetase
    Short name:
    TrpRS
    Gene namesi
    Name:WRS1
    Ordered Locus Names:YOL097C
    ORF Names:HRE432
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XV

    Organism-specific databases

    CYGDiYOL097c.
    SGDiS000005457. WRS1.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 432432Tryptophan--tRNA ligase, cytoplasmicPRO_0000136744Add
    BLAST

    Proteomic databases

    MaxQBiQ12109.
    PaxDbiQ12109.
    PeptideAtlasiQ12109.

    Expressioni

    Gene expression databases

    GenevestigatoriQ12109.

    Interactioni

    Subunit structurei

    Homodimer.

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    ARO8P530901EBI-18832,EBI-2042933
    RNR1P215241EBI-18832,EBI-15234

    Protein-protein interaction databases

    BioGridi34305. 13 interactions.
    DIPiDIP-6750N.
    IntActiQ12109. 2 interactions.
    MINTiMINT-669024.
    STRINGi4932.YOL097C.

    Structurei

    Secondary structure

    1
    432
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi47 – 548
    Helixi61 – 7111
    Helixi77 – 804
    Beta strandi83 – 897
    Helixi90 – 9910
    Beta strandi104 – 1096
    Beta strandi112 – 1143
    Helixi118 – 1203
    Helixi121 – 13414
    Beta strandi138 – 1425
    Helixi144 – 1507
    Helixi156 – 17116
    Turni172 – 1743
    Helixi177 – 1793
    Beta strandi180 – 1845
    Helixi185 – 1884
    Helixi191 – 20111
    Helixi206 – 2116
    Helixi221 – 2255
    Helixi227 – 2326
    Helixi236 – 2383
    Turni240 – 2434
    Beta strandi251 – 2566
    Helixi257 – 2593
    Helixi260 – 27314
    Beta strandi279 – 2835
    Beta strandi291 – 2955
    Helixi301 – 3033
    Helixi311 – 32111
    Helixi330 – 3367
    Turni340 – 3423
    Helixi344 – 3529
    Helixi356 – 36712
    Helixi373 – 39624
    Helixi400 – 4078

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2IP1X-ray1.80A1-432[»]
    3KT0X-ray2.10A1-432[»]
    3KT3X-ray2.60A/B/C/D1-432[»]
    3KT6X-ray2.80A/B/C/D1-432[»]
    3KT8X-ray3.00A/B/C/D1-432[»]
    ProteinModelPortaliQ12109.
    SMRiQ12109. Positions 17-425.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ12109.

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi111 – 12010"HIGH" region
    Motifi295 – 2995"KMSKS" region

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0180.
    GeneTreeiENSGT00390000000666.
    HOGENOMiHOG000224742.
    KOiK01867.
    OMAiKTFIFNN.
    OrthoDBiEOG74N5SR.

    Family and domain databases

    Gene3Di3.40.50.620. 1 hit.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002305. aa-tRNA-synth_Ic.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR002306. Trp-tRNA-ligase.
    [Graphical view]
    PANTHERiPTHR10055. PTHR10055. 1 hit.
    PfamiPF00579. tRNA-synt_1b. 1 hit.
    [Graphical view]
    PRINTSiPR01039. TRNASYNTHTRP.
    TIGRFAMsiTIGR00233. trpS. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q12109-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSNDETVEKV TQQVSELKST DVKEQVVTPW DVEGGVDEQG RAQNIDYDKL    50
    IKQFGTKPVN EETLKRFKQV TGREPHHFLR KGLFFSERDF TKILDLYEQG 100
    KPFFLYTGRG PSSDSMHLGH MIPFVFTKWL QEVFDVPLVI ELTDDEKFLF 150
    KHKLTINDVK NFARENAKDI IAVGFDPKNT FIFSDLQYMG GAFYETVVRV 200
    SRQITGSTAK AVFGFNDSDC IGKFHFASIQ IATAFPSSFP NVLGLPDKTP 250
    CLIPCAIDQD PYFRVCRDVA DKLKYSKPAL LHSRFFPALQ GSTTKMSASD 300
    DTTAIFMTDT PKQIQKKINK YAFSGGQVSA DLHRELGGNP DVDVAYQYLS 350
    FFKDDDVFLK ECYDKYKSGE LLSGEMKKLC IETLQEFVKA FQERRAQVDE 400
    ETLDKFMVPH KLVWGEKERL VAPKPKTKQE KK 432
    Length:432
    Mass (Da):49,350
    Last modified:November 1, 1996 - v1
    Checksum:iC408F169737E9736
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti87 – 871E → G in AAT92967. (PubMed:17322287)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z48149 Genomic DNA. Translation: CAA88164.1.
    Z74839 Genomic DNA. Translation: CAA99110.1.
    AY692948 Genomic DNA. Translation: AAT92967.1.
    BK006948 Genomic DNA. Translation: DAA10687.1.
    PIRiS51901.
    RefSeqiNP_014544.1. NM_001183351.1.

    Genome annotation databases

    EnsemblFungiiYOL097C; YOL097C; YOL097C.
    GeneIDi854056.
    KEGGisce:YOL097C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z48149 Genomic DNA. Translation: CAA88164.1 .
    Z74839 Genomic DNA. Translation: CAA99110.1 .
    AY692948 Genomic DNA. Translation: AAT92967.1 .
    BK006948 Genomic DNA. Translation: DAA10687.1 .
    PIRi S51901.
    RefSeqi NP_014544.1. NM_001183351.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2IP1 X-ray 1.80 A 1-432 [» ]
    3KT0 X-ray 2.10 A 1-432 [» ]
    3KT3 X-ray 2.60 A/B/C/D 1-432 [» ]
    3KT6 X-ray 2.80 A/B/C/D 1-432 [» ]
    3KT8 X-ray 3.00 A/B/C/D 1-432 [» ]
    ProteinModelPortali Q12109.
    SMRi Q12109. Positions 17-425.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 34305. 13 interactions.
    DIPi DIP-6750N.
    IntActi Q12109. 2 interactions.
    MINTi MINT-669024.
    STRINGi 4932.YOL097C.

    Proteomic databases

    MaxQBi Q12109.
    PaxDbi Q12109.
    PeptideAtlasi Q12109.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YOL097C ; YOL097C ; YOL097C .
    GeneIDi 854056.
    KEGGi sce:YOL097C.

    Organism-specific databases

    CYGDi YOL097c.
    SGDi S000005457. WRS1.

    Phylogenomic databases

    eggNOGi COG0180.
    GeneTreei ENSGT00390000000666.
    HOGENOMi HOG000224742.
    KOi K01867.
    OMAi KTFIFNN.
    OrthoDBi EOG74N5SR.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-33496-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei Q12109.
    NextBioi 975647.
    PROi Q12109.

    Gene expression databases

    Genevestigatori Q12109.

    Family and domain databases

    Gene3Di 3.40.50.620. 1 hit.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002305. aa-tRNA-synth_Ic.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR002306. Trp-tRNA-ligase.
    [Graphical view ]
    PANTHERi PTHR10055. PTHR10055. 1 hit.
    Pfami PF00579. tRNA-synt_1b. 1 hit.
    [Graphical view ]
    PRINTSi PR01039. TRNASYNTHTRP.
    TIGRFAMsi TIGR00233. trpS. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Sequence analysis of a 44 kb DNA fragment of yeast chromosome XV including the Ty1-H3 retrotransposon, the suf1(+) frameshift suppressor gene for tRNA-Gly, the yeast transfer RNA-Thr-1a and a delta element."
      Vandenbol M., Durand P., Portetelle D., Hilger F.
      Yeast 11:1069-1075(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XV."
      Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D.
      , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.
      Nature 387:98-102(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    5. "Identification and expression of the Saccharomyces cerevisiae cytoplasmic tryptophanyl-tRNA synthetase gene."
      John T.R., Ghosh M., Johnson J.D.
      Yeast 13:37-41(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    7. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiSYWC_YEAST
    AccessioniPrimary (citable) accession number: Q12109
    Secondary accession number(s): D6W1X1, E9P8Z9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 114 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 12100 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families
    4. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    5. Yeast chromosome XV
      Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

    External Data

    Dasty 3