Q12109 (SYWC_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Tryptophan--tRNA ligase, cytoplasmic
EC=6.1.1.2

Alternative name(s):
Tryptophanyl-tRNA synthetase
Short name=TrpRS

Gene names

Name:	WRS1
Ordered Locus Names:	YOL097C
ORF Names:	HRE432

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	432 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	ATP + L-tryptophan + tRNA(Trp) = AMP + diphosphate + L-tryptophyl-tRNA(Trp).
Subunit structure	Homodimer.
Subcellular location	Cytoplasm.
Miscellaneous	Present with 12100 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	tryptophanyl-tRNA aminoacylation Traceable author statement PubMed 10950302. Source: SGD
Cellular_component	cytoplasm Traceable author statement PubMed 10950302. Source: SGD
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW tryptophan-tRNA ligase activity Inferred from direct assay Ref.5. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
ARO8	P53090	1	EBI-18832,EBI-2042933
RNR1	P21524	1	EBI-18832,EBI-15234

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 432

432

Tryptophan--tRNA ligase, cytoplasmic

PRO_0000136744

Regions

Motif

111 – 120

10

"HIGH" region

Motif

295 – 299

5

"KMSKS" region

Amino acid modifications

Modified residue

20

1

Phosphothreonine Ref.7

Experimental info

Sequence conflict

87

1

E → G in AAT92967. Ref.4

Secondary structure

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432

Helix Strand Turn

Details...

Helix

47 – 54

8

Helix

61 – 71

11

Helix

77 – 80

4

Beta strand

83 – 89

7

Helix

90 – 99

10

Beta strand

104 – 109

6

Beta strand

112 – 114

3

Helix

118 – 120

3

Helix

121 – 134

14

Beta strand

138 – 142

5

Helix

144 – 150

7

Helix

156 – 171

16

Turn

172 – 174

3

Helix

177 – 179

3

Beta strand

180 – 184

5

Helix

185 – 188

4

Helix

191 – 201

11

Helix

206 – 211

6

Helix

221 – 225

5

Helix

227 – 232

6

Helix

236 – 238

3

Turn

240 – 243

4

Beta strand

251 – 256

6

Helix

257 – 259

3

Helix

260 – 273

14

Beta strand

279 – 283

5

Beta strand

291 – 295

5

Helix

301 – 303

3

Helix

311 – 321

11

Helix

330 – 336

7

Turn

340 – 342

3

Helix

344 – 352

9

Helix

356 – 367

12

Helix

373 – 396

24

Helix

400 – 407

8

Sequences

	Sequence		Length	Mass (Da)
	Q12109 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: C408F169737E9736	FASTA	432	49,350
	10 20 30 40 50 60 MSNDETVEKV TQQVSELKST DVKEQVVTPW DVEGGVDEQG RAQNIDYDKL IKQFGTKPVN 70 80 90 100 110 120 EETLKRFKQV TGREPHHFLR KGLFFSERDF TKILDLYEQG KPFFLYTGRG PSSDSMHLGH 130 140 150 160 170 180 MIPFVFTKWL QEVFDVPLVI ELTDDEKFLF KHKLTINDVK NFARENAKDI IAVGFDPKNT 190 200 210 220 230 240 FIFSDLQYMG GAFYETVVRV SRQITGSTAK AVFGFNDSDC IGKFHFASIQ IATAFPSSFP 250 260 270 280 290 300 NVLGLPDKTP CLIPCAIDQD PYFRVCRDVA DKLKYSKPAL LHSRFFPALQ GSTTKMSASD 310 320 330 340 350 360 DTTAIFMTDT PKQIQKKINK YAFSGGQVSA DLHRELGGNP DVDVAYQYLS FFKDDDVFLK 370 380 390 400 410 420 ECYDKYKSGE LLSGEMKKLC IETLQEFVKA FQERRAQVDE ETLDKFMVPH KLVWGEKERL 430 VAPKPKTKQE KK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence analysis of a 44 kb DNA fragment of yeast chromosome XV including the Ty1-H3 retrotransposon, the suf1(+) frameshift suppressor gene for tRNA-Gly, the yeast transfer RNA-Thr-1a and a delta element." Vandenbol M., Durand P., Portetelle D., Hilger F. Yeast 11:1069-1075(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XV." Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D. , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K. Nature 387:98-102(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[5]	"Identification and expression of the Saccharomyces cerevisiae cytoplasmic tryptophanyl-tRNA synthetase gene." John T.R., Ghosh M., Johnson J.D. Yeast 13:37-41(1997) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-20, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

Z48149 Genomic DNA. Translation: CAA88164.1.
Z74839 Genomic DNA. Translation: CAA99110.1.
AY692948 Genomic DNA. Translation: AAT92967.1.
BK006948 Genomic DNA. Translation: DAA10687.1.

PIR

S51901.

RefSeq

NP_014544.1. NM_001183351.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2IP1	X-ray	1.80	A	1-432	[»]
3KT0	X-ray	2.10	A	1-432	[»]
3KT3	X-ray	2.60	A/B/C/D	1-432	[»]
3KT6	X-ray	2.80	A/B/C/D	1-432	[»]
3KT8	X-ray	3.00	A/B/C/D	1-432	[»]

ProteinModelPortal

Q12109.

SMR

Q12109. Positions 17-425.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-6750N.

IntAct

Q12109. 2 interactions.

MINT

MINT-669024.

STRING

4932.YOL097C.

Proteomic databases

PaxDb

Q12109.

PeptideAtlas

Q12109.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblFungi

YOL097C; YOL097C; YOL097C.

GeneID

854056.

KEGG

sce:YOL097C.

Organism-specific databases

CYGD

YOL097c.

SGD

S000005457. WRS1.

Phylogenomic databases

eggNOG

COG0180.

GeneTree

ENSGT00390000000666.

HOGENOM

HOG000224742.

KO

K01867.

OMA

KTGGRET.

OrthoDB

EOG4RBTT4.

Gene expression databases

Genevestigator

Q12109.

GermOnline

YOL097C. Saccharomyces cerevisiae.

Family and domain databases

Gene3D

3.40.50.620. 1 hit.

InterPro

IPR001412. aa-tRNA-synth_I_CS.
IPR002305. aa-tRNA-synth_Ic.
IPR014729. Rossmann-like_a/b/a_fold.
IPR002306. Trp-tRNA-ligase.
[Graphical view]

PANTHER

PTHR10055. PTHR10055. 1 hit.

Pfam

PF00579. tRNA-synt_1b. 2 hits.
[Graphical view]

PRINTS

PR01039. TRNASYNTHTRP.

TIGRFAMs

TIGR00233. trpS. 1 hit.

PROSITE

PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

Q12109.

NextBio

975647.

Entry information

Entry name

SYWC_YEAST

Accession

Primary (citable) accession number: Q12109
Secondary accession number(s): D6W1X1, E9P8Z9

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1996
Last modified:	April 3, 2013
This is version 104 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program