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Q12109

- SYWC_YEAST

UniProt

Q12109 - SYWC_YEAST

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Protein
Tryptophan--tRNA ligase, cytoplasmic
Gene
WRS1, YOL097C, HRE432
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + L-tryptophan + tRNA(Trp) = AMP + diphosphate + L-tryptophyl-tRNA(Trp).

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. tryptophan-tRNA ligase activity Source: SGD

GO - Biological processi

  1. tryptophanyl-tRNA aminoacylation Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-33496-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Tryptophan--tRNA ligase, cytoplasmic (EC:6.1.1.2)
Alternative name(s):
Tryptophanyl-tRNA synthetase
Short name:
TrpRS
Gene namesi
Name:WRS1
Ordered Locus Names:YOL097C
ORF Names:HRE432
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XV

Organism-specific databases

CYGDiYOL097c.
SGDiS000005457. WRS1.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 432432Tryptophan--tRNA ligase, cytoplasmic
PRO_0000136744Add
BLAST

Proteomic databases

MaxQBiQ12109.
PaxDbiQ12109.
PeptideAtlasiQ12109.

Expressioni

Gene expression databases

GenevestigatoriQ12109.

Interactioni

Subunit structurei

Homodimer.

Binary interactionsi

WithEntry#Exp.IntActNotes
ARO8P530901EBI-18832,EBI-2042933
RNR1P215241EBI-18832,EBI-15234

Protein-protein interaction databases

BioGridi34305. 13 interactions.
DIPiDIP-6750N.
IntActiQ12109. 2 interactions.
MINTiMINT-669024.
STRINGi4932.YOL097C.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi47 – 548
Helixi61 – 7111
Helixi77 – 804
Beta strandi83 – 897
Helixi90 – 9910
Beta strandi104 – 1096
Beta strandi112 – 1143
Helixi118 – 1203
Helixi121 – 13414
Beta strandi138 – 1425
Helixi144 – 1507
Helixi156 – 17116
Turni172 – 1743
Helixi177 – 1793
Beta strandi180 – 1845
Helixi185 – 1884
Helixi191 – 20111
Helixi206 – 2116
Helixi221 – 2255
Helixi227 – 2326
Helixi236 – 2383
Turni240 – 2434
Beta strandi251 – 2566
Helixi257 – 2593
Helixi260 – 27314
Beta strandi279 – 2835
Beta strandi291 – 2955
Helixi301 – 3033
Helixi311 – 32111
Helixi330 – 3367
Turni340 – 3423
Helixi344 – 3529
Helixi356 – 36712
Helixi373 – 39624
Helixi400 – 4078

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2IP1X-ray1.80A1-432[»]
3KT0X-ray2.10A1-432[»]
3KT3X-ray2.60A/B/C/D1-432[»]
3KT6X-ray2.80A/B/C/D1-432[»]
3KT8X-ray3.00A/B/C/D1-432[»]
ProteinModelPortaliQ12109.
SMRiQ12109. Positions 17-425.

Miscellaneous databases

EvolutionaryTraceiQ12109.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi111 – 12010"HIGH" region
Motifi295 – 2995"KMSKS" region

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0180.
GeneTreeiENSGT00390000000666.
HOGENOMiHOG000224742.
KOiK01867.
OMAiKTFIFNN.
OrthoDBiEOG74N5SR.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002305. aa-tRNA-synth_Ic.
IPR014729. Rossmann-like_a/b/a_fold.
IPR002306. Trp-tRNA-ligase.
[Graphical view]
PANTHERiPTHR10055. PTHR10055. 1 hit.
PfamiPF00579. tRNA-synt_1b. 1 hit.
[Graphical view]
PRINTSiPR01039. TRNASYNTHTRP.
TIGRFAMsiTIGR00233. trpS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q12109-1 [UniParc]FASTAAdd to Basket

« Hide

MSNDETVEKV TQQVSELKST DVKEQVVTPW DVEGGVDEQG RAQNIDYDKL    50
IKQFGTKPVN EETLKRFKQV TGREPHHFLR KGLFFSERDF TKILDLYEQG 100
KPFFLYTGRG PSSDSMHLGH MIPFVFTKWL QEVFDVPLVI ELTDDEKFLF 150
KHKLTINDVK NFARENAKDI IAVGFDPKNT FIFSDLQYMG GAFYETVVRV 200
SRQITGSTAK AVFGFNDSDC IGKFHFASIQ IATAFPSSFP NVLGLPDKTP 250
CLIPCAIDQD PYFRVCRDVA DKLKYSKPAL LHSRFFPALQ GSTTKMSASD 300
DTTAIFMTDT PKQIQKKINK YAFSGGQVSA DLHRELGGNP DVDVAYQYLS 350
FFKDDDVFLK ECYDKYKSGE LLSGEMKKLC IETLQEFVKA FQERRAQVDE 400
ETLDKFMVPH KLVWGEKERL VAPKPKTKQE KK 432
Length:432
Mass (Da):49,350
Last modified:November 1, 1996 - v1
Checksum:iC408F169737E9736
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti87 – 871E → G in AAT92967. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z48149 Genomic DNA. Translation: CAA88164.1.
Z74839 Genomic DNA. Translation: CAA99110.1.
AY692948 Genomic DNA. Translation: AAT92967.1.
BK006948 Genomic DNA. Translation: DAA10687.1.
PIRiS51901.
RefSeqiNP_014544.1. NM_001183351.1.

Genome annotation databases

EnsemblFungiiYOL097C; YOL097C; YOL097C.
GeneIDi854056.
KEGGisce:YOL097C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z48149 Genomic DNA. Translation: CAA88164.1 .
Z74839 Genomic DNA. Translation: CAA99110.1 .
AY692948 Genomic DNA. Translation: AAT92967.1 .
BK006948 Genomic DNA. Translation: DAA10687.1 .
PIRi S51901.
RefSeqi NP_014544.1. NM_001183351.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2IP1 X-ray 1.80 A 1-432 [» ]
3KT0 X-ray 2.10 A 1-432 [» ]
3KT3 X-ray 2.60 A/B/C/D 1-432 [» ]
3KT6 X-ray 2.80 A/B/C/D 1-432 [» ]
3KT8 X-ray 3.00 A/B/C/D 1-432 [» ]
ProteinModelPortali Q12109.
SMRi Q12109. Positions 17-425.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 34305. 13 interactions.
DIPi DIP-6750N.
IntActi Q12109. 2 interactions.
MINTi MINT-669024.
STRINGi 4932.YOL097C.

Proteomic databases

MaxQBi Q12109.
PaxDbi Q12109.
PeptideAtlasi Q12109.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YOL097C ; YOL097C ; YOL097C .
GeneIDi 854056.
KEGGi sce:YOL097C.

Organism-specific databases

CYGDi YOL097c.
SGDi S000005457. WRS1.

Phylogenomic databases

eggNOGi COG0180.
GeneTreei ENSGT00390000000666.
HOGENOMi HOG000224742.
KOi K01867.
OMAi KTFIFNN.
OrthoDBi EOG74N5SR.

Enzyme and pathway databases

BioCyci YEAST:G3O-33496-MONOMER.

Miscellaneous databases

EvolutionaryTracei Q12109.
NextBioi 975647.
PROi Q12109.

Gene expression databases

Genevestigatori Q12109.

Family and domain databases

Gene3Di 3.40.50.620. 1 hit.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002305. aa-tRNA-synth_Ic.
IPR014729. Rossmann-like_a/b/a_fold.
IPR002306. Trp-tRNA-ligase.
[Graphical view ]
PANTHERi PTHR10055. PTHR10055. 1 hit.
Pfami PF00579. tRNA-synt_1b. 1 hit.
[Graphical view ]
PRINTSi PR01039. TRNASYNTHTRP.
TIGRFAMsi TIGR00233. trpS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence analysis of a 44 kb DNA fragment of yeast chromosome XV including the Ty1-H3 retrotransposon, the suf1(+) frameshift suppressor gene for tRNA-Gly, the yeast transfer RNA-Thr-1a and a delta element."
    Vandenbol M., Durand P., Portetelle D., Hilger F.
    Yeast 11:1069-1075(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XV."
    Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D.
    , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.
    Nature 387:98-102(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  5. "Identification and expression of the Saccharomyces cerevisiae cytoplasmic tryptophanyl-tRNA synthetase gene."
    John T.R., Ghosh M., Johnson J.D.
    Yeast 13:37-41(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  7. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiSYWC_YEAST
AccessioniPrimary (citable) accession number: Q12109
Secondary accession number(s): D6W1X1, E9P8Z9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: June 11, 2014
This is version 113 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 12100 molecules/cell in log phase SD medium.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  5. Yeast chromosome XV
    Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

External Data

Dasty 3

Similar proteinsi