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Protein

Tryptophan--tRNA ligase, cytoplasmic

Gene

WRS1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + L-tryptophan + tRNA(Trp) = AMP + diphosphate + L-tryptophyl-tRNA(Trp).

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • tryptophan-tRNA ligase activity Source: SGD

GO - Biological processi

  • tryptophanyl-tRNA aminoacylation Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-33496-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Tryptophan--tRNA ligase, cytoplasmic (EC:6.1.1.2)
Alternative name(s):
Tryptophanyl-tRNA synthetase
Short name:
TrpRS
Gene namesi
Name:WRS1
Ordered Locus Names:YOL097C
ORF Names:HRE432
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XV

Organism-specific databases

EuPathDBiFungiDB:YOL097C.
SGDiS000005457. WRS1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001367441 – 432Tryptophan--tRNA ligase, cytoplasmicAdd BLAST432

Proteomic databases

MaxQBiQ12109.
PRIDEiQ12109.

Interactioni

Subunit structurei

Homodimer.

Protein-protein interaction databases

BioGridi34305. 15 interactors.
DIPiDIP-6750N.
IntActiQ12109. 2 interactors.
MINTiMINT-669024.

Structurei

Secondary structure

1432
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi47 – 54Combined sources8
Helixi61 – 71Combined sources11
Helixi77 – 80Combined sources4
Beta strandi83 – 89Combined sources7
Helixi90 – 99Combined sources10
Beta strandi104 – 109Combined sources6
Beta strandi112 – 114Combined sources3
Helixi118 – 120Combined sources3
Helixi121 – 134Combined sources14
Beta strandi138 – 142Combined sources5
Helixi144 – 150Combined sources7
Helixi156 – 171Combined sources16
Turni172 – 174Combined sources3
Helixi177 – 179Combined sources3
Beta strandi180 – 184Combined sources5
Helixi185 – 188Combined sources4
Helixi191 – 201Combined sources11
Helixi206 – 211Combined sources6
Helixi221 – 225Combined sources5
Helixi227 – 232Combined sources6
Helixi236 – 238Combined sources3
Turni240 – 243Combined sources4
Beta strandi251 – 256Combined sources6
Helixi257 – 259Combined sources3
Helixi260 – 273Combined sources14
Beta strandi279 – 283Combined sources5
Beta strandi291 – 295Combined sources5
Helixi301 – 303Combined sources3
Helixi311 – 321Combined sources11
Helixi330 – 336Combined sources7
Turni340 – 342Combined sources3
Helixi344 – 352Combined sources9
Helixi356 – 367Combined sources12
Helixi373 – 396Combined sources24
Helixi400 – 407Combined sources8

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2IP1X-ray1.80A1-432[»]
3KT0X-ray2.10A1-432[»]
3KT3X-ray2.60A/B/C/D1-432[»]
3KT6X-ray2.80A/B/C/D1-432[»]
3KT8X-ray3.00A/B/C/D1-432[»]
ProteinModelPortaliQ12109.
SMRiQ12109.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ12109.

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi111 – 120"HIGH" region10
Motifi295 – 299"KMSKS" region5

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00390000000666.
HOGENOMiHOG000224742.
InParanoidiQ12109.
KOiK01867.
OMAiQNARDII.
OrthoDBiEOG092C2ZCA.

Family and domain databases

CDDicd00806. TrpRS_core. 1 hit.
Gene3Di3.40.50.620. 1 hit.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002305. aa-tRNA-synth_Ic.
IPR014729. Rossmann-like_a/b/a_fold.
IPR002306. Trp-tRNA-ligase.
[Graphical view]
PANTHERiPTHR10055. PTHR10055. 1 hit.
PfamiPF00579. tRNA-synt_1b. 1 hit.
[Graphical view]
PRINTSiPR01039. TRNASYNTHTRP.
TIGRFAMsiTIGR00233. trpS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q12109-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSNDETVEKV TQQVSELKST DVKEQVVTPW DVEGGVDEQG RAQNIDYDKL
60 70 80 90 100
IKQFGTKPVN EETLKRFKQV TGREPHHFLR KGLFFSERDF TKILDLYEQG
110 120 130 140 150
KPFFLYTGRG PSSDSMHLGH MIPFVFTKWL QEVFDVPLVI ELTDDEKFLF
160 170 180 190 200
KHKLTINDVK NFARENAKDI IAVGFDPKNT FIFSDLQYMG GAFYETVVRV
210 220 230 240 250
SRQITGSTAK AVFGFNDSDC IGKFHFASIQ IATAFPSSFP NVLGLPDKTP
260 270 280 290 300
CLIPCAIDQD PYFRVCRDVA DKLKYSKPAL LHSRFFPALQ GSTTKMSASD
310 320 330 340 350
DTTAIFMTDT PKQIQKKINK YAFSGGQVSA DLHRELGGNP DVDVAYQYLS
360 370 380 390 400
FFKDDDVFLK ECYDKYKSGE LLSGEMKKLC IETLQEFVKA FQERRAQVDE
410 420 430
ETLDKFMVPH KLVWGEKERL VAPKPKTKQE KK
Length:432
Mass (Da):49,350
Last modified:November 1, 1996 - v1
Checksum:iC408F169737E9736
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti87E → G in AAT92967 (PubMed:17322287).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z48149 Genomic DNA. Translation: CAA88164.1.
Z74839 Genomic DNA. Translation: CAA99110.1.
AY692948 Genomic DNA. Translation: AAT92967.1.
BK006948 Genomic DNA. Translation: DAA10687.1.
PIRiS51901.
RefSeqiNP_014544.1. NM_001183351.1.

Genome annotation databases

EnsemblFungiiYOL097C; YOL097C; YOL097C.
GeneIDi854056.
KEGGisce:YOL097C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z48149 Genomic DNA. Translation: CAA88164.1.
Z74839 Genomic DNA. Translation: CAA99110.1.
AY692948 Genomic DNA. Translation: AAT92967.1.
BK006948 Genomic DNA. Translation: DAA10687.1.
PIRiS51901.
RefSeqiNP_014544.1. NM_001183351.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2IP1X-ray1.80A1-432[»]
3KT0X-ray2.10A1-432[»]
3KT3X-ray2.60A/B/C/D1-432[»]
3KT6X-ray2.80A/B/C/D1-432[»]
3KT8X-ray3.00A/B/C/D1-432[»]
ProteinModelPortaliQ12109.
SMRiQ12109.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34305. 15 interactors.
DIPiDIP-6750N.
IntActiQ12109. 2 interactors.
MINTiMINT-669024.

Proteomic databases

MaxQBiQ12109.
PRIDEiQ12109.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYOL097C; YOL097C; YOL097C.
GeneIDi854056.
KEGGisce:YOL097C.

Organism-specific databases

EuPathDBiFungiDB:YOL097C.
SGDiS000005457. WRS1.

Phylogenomic databases

GeneTreeiENSGT00390000000666.
HOGENOMiHOG000224742.
InParanoidiQ12109.
KOiK01867.
OMAiQNARDII.
OrthoDBiEOG092C2ZCA.

Enzyme and pathway databases

BioCyciYEAST:G3O-33496-MONOMER.

Miscellaneous databases

EvolutionaryTraceiQ12109.
PROiQ12109.

Family and domain databases

CDDicd00806. TrpRS_core. 1 hit.
Gene3Di3.40.50.620. 1 hit.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002305. aa-tRNA-synth_Ic.
IPR014729. Rossmann-like_a/b/a_fold.
IPR002306. Trp-tRNA-ligase.
[Graphical view]
PANTHERiPTHR10055. PTHR10055. 1 hit.
PfamiPF00579. tRNA-synt_1b. 1 hit.
[Graphical view]
PRINTSiPR01039. TRNASYNTHTRP.
TIGRFAMsiTIGR00233. trpS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSYWC_YEAST
AccessioniPrimary (citable) accession number: Q12109
Secondary accession number(s): D6W1X1, E9P8Z9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: November 30, 2016
This is version 132 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 12100 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  5. Yeast chromosome XV
    Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.