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Reviewed, UniProtKB/Swiss-Prot Q12109 (SYWC_YEAST)

Last modified June 16, 2009. Version 69. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Tryptophanyl-tRNA synthetase, cytoplasmic
    EC=6.1.1.2
Alternative name(s):
    Tryptophan--tRNA ligase
      Short name=TrpRS
Gene names
Name: WRS1
Ordered Locus Names: YOL097C
ORF Names: HRE432
OrganismSaccharomyces cerevisiae (Baker's yeast) [Complete proteome]
Taxonomic identifier4932 [NCBI]
Taxonomic lineageEukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

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Protein attributes

Sequence length432 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Catalytic activity

ATP + L-tryptophan + tRNA(Trp) = AMP + diphosphate + L-tryptophyl-tRNA(Trp).

Subunit structure

Homodimer.

Subcellular location

Cytoplasm.

Miscellaneous

Present with 12100 molecules/cell in log phase SD medium. Ref.4

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

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Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   PTMPhosphoprotein
   Technical term3D-structure
Complete proteome
Gene Ontology (GO)
   Biological processtryptophanyl-tRNA aminoacylation

Traceable author statement. Source: SGD

   Cellular componentcytoplasm

Traceable author statement. Source: SGD

   Molecular functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

protein binding

Inferred from physical interaction. Source: IntAct

tryptophan-tRNA ligase activity Ref.3

Inferred from direct assay. Source: SGD

Complete GO annotation...

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Binary interactions

With

Entry

#Exp.

IntAct

Notes

ARO8P530901EBI-18832,EBI-2042933
RNR1P215241EBI-18832,EBI-15234

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 432432Tryptophanyl-tRNA synthetase, cytoplasmic
PRO_0000136744

Regions

Motif111 – 12010"HIGH" region
Motif295 – 2995"KMSKS" region

Amino acid modifications

Modified residue201Phosphothreonine Ref.5

Secondary structure

1 ............................................................ 432
Helix Strand Turn

Details...

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Sequences

Sequence LengthMass (Da)Tools
Q12109-1 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: C408F169737E9736

FASTA43249,350
        10         20         30         40         50         60 
MSNDETVEKV TQQVSELKST DVKEQVVTPW DVEGGVDEQG RAQNIDYDKL IKQFGTKPVN 

        70         80         90        100        110        120 
EETLKRFKQV TGREPHHFLR KGLFFSERDF TKILDLYEQG KPFFLYTGRG PSSDSMHLGH 

       130        140        150        160        170        180 
MIPFVFTKWL QEVFDVPLVI ELTDDEKFLF KHKLTINDVK NFARENAKDI IAVGFDPKNT 

       190        200        210        220        230        240 
FIFSDLQYMG GAFYETVVRV SRQITGSTAK AVFGFNDSDC IGKFHFASIQ IATAFPSSFP 

       250        260        270        280        290        300 
NVLGLPDKTP CLIPCAIDQD PYFRVCRDVA DKLKYSKPAL LHSRFFPALQ GSTTKMSASD 

       310        320        330        340        350        360 
DTTAIFMTDT PKQIQKKINK YAFSGGQVSA DLHRELGGNP DVDVAYQYLS FFKDDDVFLK 

       370        380        390        400        410        420 
ECYDKYKSGE LLSGEMKKLC IETLQEFVKA FQERRAQVDE ETLDKFMVPH KLVWGEKERL 

       430 
VAPKPKTKQE KK

« Hide

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References

« Hide 'large scale' references
[1]"Sequence analysis of a 44 kb DNA fragment of yeast chromosome XV including the Ty1-H3 retrotransposon, the suf1(+) frameshift suppressor gene for tRNA-Gly, the yeast transfer RNA-Thr-1a and a delta element."
Vandenbol M., Durand P., Portetelle D., Hilger F.
Yeast 11:1069-1075(1995) [PubMed: 7502582] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XV."
Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D. expand/collapse author list , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.
Nature 387:98-102(1997) [PubMed: 9169874] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 96604 / S288c / FY1679.
[3]"Identification and expression of the Saccharomyces cerevisiae cytoplasmic tryptophanyl-tRNA synthetase gene."
John T.R., Ghosh M., Johnson J.D.
Yeast 13:37-41(1997) [PubMed: 9046085] [Abstract]
Cited for: FUNCTION.
[4]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed: 14562106] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[5]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-20, MASS SPECTROMETRY.
+Additional computationally mapped references.

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Cross-references

Sequence databases

Z48149 Genomic DNA. Translation: CAA88164.1.
Z74839 Genomic DNA. Translation: CAA99110.1.
PIRS51901.
RefSeqNP_014544.1.

3D structure databases

EntryMethodResolution (Å)ChainPositionsPDBsum
2IP1X-ray1.80A1-432[»]
ModBaseSearch...

Protein-protein interaction databases

DIPDIP:6750N.
IntActQ12109. 7 interactions.

Proteomic databases

PeptideAtlasQ12109.

Genome annotation databases

EnsemblYOL097C. Saccharomyces cerevisiae. [Contig view]
GeneID854056.
GenomeReviewsGene locus YOL097C in contig Y13140_GR.
KEGGsce:YOL097C.
NMPDRfig|4932.3.peg.5633.

Organism-specific databases

CYGDYOL097c.
SGDS000005457. WRS1.
Yeast-GFPSearch...

Phylogenomic databases

HOGENOMQ12109.
OMAQ12109. FSDLEYM.

Enzyme and pathway databases

BRENDA6.1.1.2. 250.

Gene expression databases

GermOnlineYOL097C. Saccharomyces cerevisiae.

Family and domain databases

InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002305. aa-tRNA-synth_Ib.
IPR014729. Rossmann-like_a/b/a_fold.
IPR002306. Trp-tRNA-synth_Ib.
[Graphical view]
Gene3DG3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
PANTHERPTHR10055. Trp_tRNA-synt_1b. 1 hit.
PfamPF00579. tRNA-synt_1b. 1 hit.
[Graphical view]
PRINTSPR01039. TRNASYNTHTRP.
TIGRFAMsTIGR00233. trpS. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

NextBio975647.

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Entry information

Entry nameSYWC_YEAST
AccessionPrimary (citable) accession number: Q12109
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: June 16, 2009
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectFPAP (Fungal Proteome Annotation Project)

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Relevant documents

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents