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Protein

Tryptophan--tRNA ligase, cytoplasmic

Gene

WRS1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + L-tryptophan + tRNA(Trp) = AMP + diphosphate + L-tryptophyl-tRNA(Trp).

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. tryptophan-tRNA ligase activity Source: SGD

GO - Biological processi

  1. tryptophanyl-tRNA aminoacylation Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-33496-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Tryptophan--tRNA ligase, cytoplasmic (EC:6.1.1.2)
Alternative name(s):
Tryptophanyl-tRNA synthetase
Short name:
TrpRS
Gene namesi
Name:WRS1
Ordered Locus Names:YOL097C
ORF Names:HRE432
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XV

Organism-specific databases

CYGDiYOL097c.
SGDiS000005457. WRS1.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 432432Tryptophan--tRNA ligase, cytoplasmicPRO_0000136744Add
BLAST

Proteomic databases

MaxQBiQ12109.
PaxDbiQ12109.
PeptideAtlasiQ12109.

Expressioni

Gene expression databases

GenevestigatoriQ12109.

Interactioni

Subunit structurei

Homodimer.

Binary interactionsi

WithEntry#Exp.IntActNotes
ARO8P530901EBI-18832,EBI-2042933
RNR1P215241EBI-18832,EBI-15234

Protein-protein interaction databases

BioGridi34305. 14 interactions.
DIPiDIP-6750N.
IntActiQ12109. 2 interactions.
MINTiMINT-669024.
STRINGi4932.YOL097C.

Structurei

Secondary structure

1
432
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi47 – 548Combined sources
Helixi61 – 7111Combined sources
Helixi77 – 804Combined sources
Beta strandi83 – 897Combined sources
Helixi90 – 9910Combined sources
Beta strandi104 – 1096Combined sources
Beta strandi112 – 1143Combined sources
Helixi118 – 1203Combined sources
Helixi121 – 13414Combined sources
Beta strandi138 – 1425Combined sources
Helixi144 – 1507Combined sources
Helixi156 – 17116Combined sources
Turni172 – 1743Combined sources
Helixi177 – 1793Combined sources
Beta strandi180 – 1845Combined sources
Helixi185 – 1884Combined sources
Helixi191 – 20111Combined sources
Helixi206 – 2116Combined sources
Helixi221 – 2255Combined sources
Helixi227 – 2326Combined sources
Helixi236 – 2383Combined sources
Turni240 – 2434Combined sources
Beta strandi251 – 2566Combined sources
Helixi257 – 2593Combined sources
Helixi260 – 27314Combined sources
Beta strandi279 – 2835Combined sources
Beta strandi291 – 2955Combined sources
Helixi301 – 3033Combined sources
Helixi311 – 32111Combined sources
Helixi330 – 3367Combined sources
Turni340 – 3423Combined sources
Helixi344 – 3529Combined sources
Helixi356 – 36712Combined sources
Helixi373 – 39624Combined sources
Helixi400 – 4078Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2IP1X-ray1.80A1-432[»]
3KT0X-ray2.10A1-432[»]
3KT3X-ray2.60A/B/C/D1-432[»]
3KT6X-ray2.80A/B/C/D1-432[»]
3KT8X-ray3.00A/B/C/D1-432[»]
ProteinModelPortaliQ12109.
SMRiQ12109. Positions 17-425.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ12109.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi111 – 12010"HIGH" region
Motifi295 – 2995"KMSKS" region

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0180.
GeneTreeiENSGT00390000000666.
HOGENOMiHOG000224742.
InParanoidiQ12109.
KOiK01867.
OMAiKPETAIY.
OrthoDBiEOG74N5SR.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002305. aa-tRNA-synth_Ic.
IPR014729. Rossmann-like_a/b/a_fold.
IPR002306. Trp-tRNA-ligase.
[Graphical view]
PANTHERiPTHR10055. PTHR10055. 1 hit.
PfamiPF00579. tRNA-synt_1b. 1 hit.
[Graphical view]
PRINTSiPR01039. TRNASYNTHTRP.
TIGRFAMsiTIGR00233. trpS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q12109-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSNDETVEKV TQQVSELKST DVKEQVVTPW DVEGGVDEQG RAQNIDYDKL
60 70 80 90 100
IKQFGTKPVN EETLKRFKQV TGREPHHFLR KGLFFSERDF TKILDLYEQG
110 120 130 140 150
KPFFLYTGRG PSSDSMHLGH MIPFVFTKWL QEVFDVPLVI ELTDDEKFLF
160 170 180 190 200
KHKLTINDVK NFARENAKDI IAVGFDPKNT FIFSDLQYMG GAFYETVVRV
210 220 230 240 250
SRQITGSTAK AVFGFNDSDC IGKFHFASIQ IATAFPSSFP NVLGLPDKTP
260 270 280 290 300
CLIPCAIDQD PYFRVCRDVA DKLKYSKPAL LHSRFFPALQ GSTTKMSASD
310 320 330 340 350
DTTAIFMTDT PKQIQKKINK YAFSGGQVSA DLHRELGGNP DVDVAYQYLS
360 370 380 390 400
FFKDDDVFLK ECYDKYKSGE LLSGEMKKLC IETLQEFVKA FQERRAQVDE
410 420 430
ETLDKFMVPH KLVWGEKERL VAPKPKTKQE KK
Length:432
Mass (Da):49,350
Last modified:November 1, 1996 - v1
Checksum:iC408F169737E9736
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti87 – 871E → G in AAT92967 (PubMed:17322287).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z48149 Genomic DNA. Translation: CAA88164.1.
Z74839 Genomic DNA. Translation: CAA99110.1.
AY692948 Genomic DNA. Translation: AAT92967.1.
BK006948 Genomic DNA. Translation: DAA10687.1.
PIRiS51901.
RefSeqiNP_014544.1. NM_001183351.1.

Genome annotation databases

EnsemblFungiiYOL097C; YOL097C; YOL097C.
GeneIDi854056.
KEGGisce:YOL097C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z48149 Genomic DNA. Translation: CAA88164.1.
Z74839 Genomic DNA. Translation: CAA99110.1.
AY692948 Genomic DNA. Translation: AAT92967.1.
BK006948 Genomic DNA. Translation: DAA10687.1.
PIRiS51901.
RefSeqiNP_014544.1. NM_001183351.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2IP1X-ray1.80A1-432[»]
3KT0X-ray2.10A1-432[»]
3KT3X-ray2.60A/B/C/D1-432[»]
3KT6X-ray2.80A/B/C/D1-432[»]
3KT8X-ray3.00A/B/C/D1-432[»]
ProteinModelPortaliQ12109.
SMRiQ12109. Positions 17-425.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34305. 14 interactions.
DIPiDIP-6750N.
IntActiQ12109. 2 interactions.
MINTiMINT-669024.
STRINGi4932.YOL097C.

Proteomic databases

MaxQBiQ12109.
PaxDbiQ12109.
PeptideAtlasiQ12109.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYOL097C; YOL097C; YOL097C.
GeneIDi854056.
KEGGisce:YOL097C.

Organism-specific databases

CYGDiYOL097c.
SGDiS000005457. WRS1.

Phylogenomic databases

eggNOGiCOG0180.
GeneTreeiENSGT00390000000666.
HOGENOMiHOG000224742.
InParanoidiQ12109.
KOiK01867.
OMAiKPETAIY.
OrthoDBiEOG74N5SR.

Enzyme and pathway databases

BioCyciYEAST:G3O-33496-MONOMER.

Miscellaneous databases

EvolutionaryTraceiQ12109.
NextBioi975647.
PROiQ12109.

Gene expression databases

GenevestigatoriQ12109.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002305. aa-tRNA-synth_Ic.
IPR014729. Rossmann-like_a/b/a_fold.
IPR002306. Trp-tRNA-ligase.
[Graphical view]
PANTHERiPTHR10055. PTHR10055. 1 hit.
PfamiPF00579. tRNA-synt_1b. 1 hit.
[Graphical view]
PRINTSiPR01039. TRNASYNTHTRP.
TIGRFAMsiTIGR00233. trpS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence analysis of a 44 kb DNA fragment of yeast chromosome XV including the Ty1-H3 retrotransposon, the suf1(+) frameshift suppressor gene for tRNA-Gly, the yeast transfer RNA-Thr-1a and a delta element."
    Vandenbol M., Durand P., Portetelle D., Hilger F.
    Yeast 11:1069-1075(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XV."
    Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D.
    , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.
    Nature 387:98-102(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  5. "Identification and expression of the Saccharomyces cerevisiae cytoplasmic tryptophanyl-tRNA synthetase gene."
    John T.R., Ghosh M., Johnson J.D.
    Yeast 13:37-41(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  7. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiSYWC_YEAST
AccessioniPrimary (citable) accession number: Q12109
Secondary accession number(s): D6W1X1, E9P8Z9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: January 7, 2015
This is version 117 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 12100 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  5. Yeast chromosome XV
    Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.