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Protein

Tryptophan--tRNA ligase, cytoplasmic

Gene

WRS1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Miscellaneous

Present with 12100 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

ATP + L-tryptophan + tRNA(Trp) = AMP + diphosphate + L-tryptophyl-tRNA(Trp).

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • tryptophan-tRNA ligase activity Source: SGD

GO - Biological processi

  • tryptophanyl-tRNA aminoacylation Source: SGD

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-33496-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Tryptophan--tRNA ligase, cytoplasmic (EC:6.1.1.2)
Alternative name(s):
Tryptophanyl-tRNA synthetase
Short name:
TrpRS
Gene namesi
Name:WRS1
Ordered Locus Names:YOL097C
ORF Names:HRE432
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XV

Organism-specific databases

EuPathDBiFungiDB:YOL097C
SGDiS000005457 WRS1

Subcellular locationi

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001367441 – 432Tryptophan--tRNA ligase, cytoplasmicAdd BLAST432

Proteomic databases

MaxQBiQ12109
PaxDbiQ12109
PRIDEiQ12109

Interactioni

Subunit structurei

Homodimer.

Protein-protein interaction databases

BioGridi34305, 234 interactors
DIPiDIP-6750N
IntActiQ12109, 2 interactors
MINTiQ12109
STRINGi4932.YOL097C

Structurei

Secondary structure

1432
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi47 – 54Combined sources8
Helixi61 – 71Combined sources11
Helixi77 – 80Combined sources4
Beta strandi83 – 89Combined sources7
Helixi90 – 99Combined sources10
Beta strandi104 – 109Combined sources6
Beta strandi112 – 114Combined sources3
Helixi118 – 120Combined sources3
Helixi121 – 134Combined sources14
Beta strandi138 – 142Combined sources5
Helixi144 – 150Combined sources7
Helixi156 – 171Combined sources16
Turni172 – 174Combined sources3
Helixi177 – 179Combined sources3
Beta strandi180 – 184Combined sources5
Helixi185 – 188Combined sources4
Helixi191 – 201Combined sources11
Helixi206 – 211Combined sources6
Helixi221 – 225Combined sources5
Helixi227 – 232Combined sources6
Helixi236 – 238Combined sources3
Turni240 – 243Combined sources4
Beta strandi251 – 256Combined sources6
Helixi257 – 259Combined sources3
Helixi260 – 273Combined sources14
Beta strandi279 – 283Combined sources5
Beta strandi291 – 295Combined sources5
Helixi301 – 303Combined sources3
Helixi311 – 321Combined sources11
Helixi330 – 336Combined sources7
Turni340 – 342Combined sources3
Helixi344 – 352Combined sources9
Helixi356 – 367Combined sources12
Helixi373 – 396Combined sources24
Helixi400 – 407Combined sources8

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2IP1X-ray1.80A1-432[»]
3KT0X-ray2.10A1-432[»]
3KT3X-ray2.60A/B/C/D1-432[»]
3KT6X-ray2.80A/B/C/D1-432[»]
3KT8X-ray3.00A/B/C/D1-432[»]
ProteinModelPortaliQ12109
SMRiQ12109
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ12109

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi111 – 120"HIGH" region10
Motifi295 – 299"KMSKS" region5

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00390000000666
HOGENOMiHOG000224742
InParanoidiQ12109
KOiK01867
OMAiADILHPQ
OrthoDBiEOG092C2ZCA

Family and domain databases

CDDicd00806 TrpRS_core, 1 hit
Gene3Di3.40.50.620, 1 hit
InterProiView protein in InterPro
IPR001412 aa-tRNA-synth_I_CS
IPR002305 aa-tRNA-synth_Ic
IPR014729 Rossmann-like_a/b/a_fold
IPR002306 Trp-tRNA-ligase
PfamiView protein in Pfam
PF00579 tRNA-synt_1b, 1 hit
PRINTSiPR01039 TRNASYNTHTRP
TIGRFAMsiTIGR00233 trpS, 1 hit
PROSITEiView protein in PROSITE
PS00178 AA_TRNA_LIGASE_I, 1 hit

Sequencei

Sequence statusi: Complete.

Q12109-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSNDETVEKV TQQVSELKST DVKEQVVTPW DVEGGVDEQG RAQNIDYDKL
60 70 80 90 100
IKQFGTKPVN EETLKRFKQV TGREPHHFLR KGLFFSERDF TKILDLYEQG
110 120 130 140 150
KPFFLYTGRG PSSDSMHLGH MIPFVFTKWL QEVFDVPLVI ELTDDEKFLF
160 170 180 190 200
KHKLTINDVK NFARENAKDI IAVGFDPKNT FIFSDLQYMG GAFYETVVRV
210 220 230 240 250
SRQITGSTAK AVFGFNDSDC IGKFHFASIQ IATAFPSSFP NVLGLPDKTP
260 270 280 290 300
CLIPCAIDQD PYFRVCRDVA DKLKYSKPAL LHSRFFPALQ GSTTKMSASD
310 320 330 340 350
DTTAIFMTDT PKQIQKKINK YAFSGGQVSA DLHRELGGNP DVDVAYQYLS
360 370 380 390 400
FFKDDDVFLK ECYDKYKSGE LLSGEMKKLC IETLQEFVKA FQERRAQVDE
410 420 430
ETLDKFMVPH KLVWGEKERL VAPKPKTKQE KK
Length:432
Mass (Da):49,350
Last modified:November 1, 1996 - v1
Checksum:iC408F169737E9736
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti87E → G in AAT92967 (PubMed:17322287).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z48149 Genomic DNA Translation: CAA88164.1
Z74839 Genomic DNA Translation: CAA99110.1
AY692948 Genomic DNA Translation: AAT92967.1
BK006948 Genomic DNA Translation: DAA10687.1
PIRiS51901
RefSeqiNP_014544.1, NM_001183351.1

Genome annotation databases

EnsemblFungiiYOL097C; YOL097C; YOL097C
GeneIDi854056
KEGGisce:YOL097C

Similar proteinsi

Entry informationi

Entry nameiSYWC_YEAST
AccessioniPrimary (citable) accession number: Q12109
Secondary accession number(s): D6W1X1, E9P8Z9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: May 23, 2018
This is version 144 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

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