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Q12109 (SYWC_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 113. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Tryptophan--tRNA ligase, cytoplasmic

EC=6.1.1.2
Alternative name(s):
Tryptophanyl-tRNA synthetase
Short name=TrpRS
Gene names
Name:WRS1
Ordered Locus Names:YOL097C
ORF Names:HRE432
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length432 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

ATP + L-tryptophan + tRNA(Trp) = AMP + diphosphate + L-tryptophyl-tRNA(Trp).

Subunit structure

Homodimer.

Subcellular location

Cytoplasm.

Miscellaneous

Present with 12100 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical term3D-structure
Complete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processtryptophanyl-tRNA aminoacylation

Traceable author statement PubMed 10950302. Source: SGD

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

tryptophan-tRNA ligase activity

Inferred from direct assay Ref.5. Source: SGD

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

ARO8P530901EBI-18832,EBI-2042933
RNR1P215241EBI-18832,EBI-15234

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 432432Tryptophan--tRNA ligase, cytoplasmic
PRO_0000136744

Regions

Motif111 – 12010"HIGH" region
Motif295 – 2995"KMSKS" region

Experimental info

Sequence conflict871E → G in AAT92967. Ref.4

Secondary structure

................................................................ 432
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q12109 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: C408F169737E9736

FASTA43249,350
        10         20         30         40         50         60 
MSNDETVEKV TQQVSELKST DVKEQVVTPW DVEGGVDEQG RAQNIDYDKL IKQFGTKPVN 

        70         80         90        100        110        120 
EETLKRFKQV TGREPHHFLR KGLFFSERDF TKILDLYEQG KPFFLYTGRG PSSDSMHLGH 

       130        140        150        160        170        180 
MIPFVFTKWL QEVFDVPLVI ELTDDEKFLF KHKLTINDVK NFARENAKDI IAVGFDPKNT 

       190        200        210        220        230        240 
FIFSDLQYMG GAFYETVVRV SRQITGSTAK AVFGFNDSDC IGKFHFASIQ IATAFPSSFP 

       250        260        270        280        290        300 
NVLGLPDKTP CLIPCAIDQD PYFRVCRDVA DKLKYSKPAL LHSRFFPALQ GSTTKMSASD 

       310        320        330        340        350        360 
DTTAIFMTDT PKQIQKKINK YAFSGGQVSA DLHRELGGNP DVDVAYQYLS FFKDDDVFLK 

       370        380        390        400        410        420 
ECYDKYKSGE LLSGEMKKLC IETLQEFVKA FQERRAQVDE ETLDKFMVPH KLVWGEKERL 

       430 
VAPKPKTKQE KK 

« Hide

References

« Hide 'large scale' references
[1]"Sequence analysis of a 44 kb DNA fragment of yeast chromosome XV including the Ty1-H3 retrotransposon, the suf1(+) frameshift suppressor gene for tRNA-Gly, the yeast transfer RNA-Thr-1a and a delta element."
Vandenbol M., Durand P., Portetelle D., Hilger F.
Yeast 11:1069-1075(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XV."
Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D. expand/collapse author list , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.
Nature 387:98-102(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae."
Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. expand/collapse author list , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J.
Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[5]"Identification and expression of the Saccharomyces cerevisiae cytoplasmic tryptophanyl-tRNA synthetase gene."
John T.R., Ghosh M., Johnson J.D.
Yeast 13:37-41(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[6]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z48149 Genomic DNA. Translation: CAA88164.1.
Z74839 Genomic DNA. Translation: CAA99110.1.
AY692948 Genomic DNA. Translation: AAT92967.1.
BK006948 Genomic DNA. Translation: DAA10687.1.
PIRS51901.
RefSeqNP_014544.1. NM_001183351.1.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2IP1X-ray1.80A1-432[»]
3KT0X-ray2.10A1-432[»]
3KT3X-ray2.60A/B/C/D1-432[»]
3KT6X-ray2.80A/B/C/D1-432[»]
3KT8X-ray3.00A/B/C/D1-432[»]
ProteinModelPortalQ12109.
SMRQ12109. Positions 17-425.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid34305. 13 interactions.
DIPDIP-6750N.
IntActQ12109. 2 interactions.
MINTMINT-669024.
STRING4932.YOL097C.

Proteomic databases

MaxQBQ12109.
PaxDbQ12109.
PeptideAtlasQ12109.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYOL097C; YOL097C; YOL097C.
GeneID854056.
KEGGsce:YOL097C.

Organism-specific databases

CYGDYOL097c.
SGDS000005457. WRS1.

Phylogenomic databases

eggNOGCOG0180.
GeneTreeENSGT00390000000666.
HOGENOMHOG000224742.
KOK01867.
OMAKTFIFNN.
OrthoDBEOG74N5SR.

Enzyme and pathway databases

BioCycYEAST:G3O-33496-MONOMER.

Gene expression databases

GenevestigatorQ12109.

Family and domain databases

Gene3D3.40.50.620. 1 hit.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002305. aa-tRNA-synth_Ic.
IPR014729. Rossmann-like_a/b/a_fold.
IPR002306. Trp-tRNA-ligase.
[Graphical view]
PANTHERPTHR10055. PTHR10055. 1 hit.
PfamPF00579. tRNA-synt_1b. 1 hit.
[Graphical view]
PRINTSPR01039. TRNASYNTHTRP.
TIGRFAMsTIGR00233. trpS. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceQ12109.
NextBio975647.
PROQ12109.

Entry information

Entry nameSYWC_YEAST
AccessionPrimary (citable) accession number: Q12109
Secondary accession number(s): D6W1X1, E9P8Z9
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: June 11, 2014
This is version 113 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XV

Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries