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Q12100 (RTK1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 112. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Probable serine/threonine-protein kinase RTK1

EC=2.7.11.1
Alternative name(s):
Ribosome biogenesis and tRNA synthetase-associated kinase 1
Gene names
Name:RTK1
Ordered Locus Names:YDL025C
ORF Names:D2810
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length620 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Probable serine/threonine-protein kinase that may be involved in ribosome biogenesis. Ref.4

Catalytic activity

ATP + a protein = ADP + a phosphoprotein.

Subunit structure

Interacts with ribosome biogenesis factors ARC1, CKA2 and GUS1.

Induction

By the alkylating agent methyl methanesulfonate (MMS). Ref.5

Miscellaneous

Present with 861 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.

Contains 1 protein kinase domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 620620Probable serine/threonine-protein kinase RTK1
PRO_0000248408

Regions

Domain302 – 575274Protein kinase
Nucleotide binding308 – 3169ATP By similarity

Sites

Active site4301Proton acceptor By similarity
Binding site3301ATP By similarity

Amino acid modifications

Modified residue581Phosphothreonine Ref.7
Modified residue601Phosphoserine Ref.7
Modified residue2161Phosphoserine Ref.7

Sequences

Sequence LengthMass (Da)Tools
Q12100 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: AD6160F19F3B75CF

FASTA62069,615
        10         20         30         40         50         60 
MVKETPLHSS SSTSLSSLFR PTKLKNLSAK IFNGGGNQSY SKTDDVSRSS SRSSKKNTDS 

        70         80         90        100        110        120 
DQEDQIKYNK PNDRRSTIGK SPQGNGALSK ESHVVASSTL TGISPTSAKK APIDYSPSRP 

       130        140        150        160        170        180 
LPNNHNPVRT GHTVPHLPHS IHNPINYIHQ GSKDAFHHPH PVRSTAHSNI STVSSAKSDT 

       190        200        210        220        230        240 
PSSNLSYQAH MHPVEILQKQ IEDKHFMDSQ ASTPGSVELQ HNSSSGSDDT SSRKKKSLRL 

       250        260        270        280        290        300 
TRFFKKIHND YHDNHHHHHH HNRGSTPTKP KLNLNTNENI VESNGKALYE TDNPVELLEK 

       310        320        330        340        350        360 
YGIPGRKLGE GASGSVSVVE RTDGKLFACK MFRKPHLNNE GTNQSQLANY SKKVTTEFCI 

       370        380        390        400        410        420 
GSTLHHENIV ETLDMLTEGD TYLLVMEYAP YDFFNLVMSN LMTQDEVNCY FKQLCHGVNY 

       430        440        450        460        470        480 
LHSMGLAHRD LKLDNCVVTK DGILKLIDFG SAVVFQYPYE DTIVKSHGIV GSDPYLAPEL 

       490        500        510        520        530        540 
LKQTSYDPRV ADVWSIAIIF YCMVLKRFPW KAPKKSFNSF RLFTEEPEDE DDIVRGPNKI 

       550        560        570        580        590        600 
LRLLPRHSRT IIGRMLALEP KQRVLMNDVV KDDWLVSVPS CEVDPTSGDL VEKPKNHKHH 

       610        620 
LVTEEELNEL TKQHGNKDSN 

« Hide

References

« Hide 'large scale' references
[1]"The nucleotide sequence of Saccharomyces cerevisiae chromosome IV."
Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T. expand/collapse author list , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P.
Nature 387:75-78(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[3]"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae."
Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. expand/collapse author list , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J.
Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]"The protein kinases of budding yeast: six score and more."
Hunter T., Plowman G.D.
Trends Biochem. Sci. 22:18-22(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: PREDICTION OF FUNCTION.
[5]"Global response of Saccharomyces cerevisiae to an alkylating agent."
Jelinsky S.A., Samson L.D.
Proc. Natl. Acad. Sci. U.S.A. 96:1486-1491(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: INDUCTION.
[6]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-58; SER-60 AND SER-216, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[8]"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[9]"A global protein kinase and phosphatase interaction network in yeast."
Breitkreutz A., Choi H., Sharom J.R., Boucher L., Neduva V., Larsen B., Lin Z.Y., Breitkreutz B.J., Stark C., Liu G., Ahn J., Dewar-Darch D., Reguly T., Tang X., Almeida R., Qin Z.S., Pawson T., Gingras A.C., Nesvizhskii A.I., Tyers M.
Science 328:1043-1046(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION [LARGE SCALE ANALYSIS] WITH ARC1; CKA2 AND GUS1.
[10]"Sites of ubiquitin attachment in Saccharomyces cerevisiae."
Starita L.M., Lo R.S., Eng J.K., von Haller P.D., Fields S.
Proteomics 12:236-240(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z74073 Genomic DNA. Translation: CAA98584.1.
Z48432 Genomic DNA. Translation: CAA88334.1.
AY692716 Genomic DNA. Translation: AAT92735.1.
BK006938 Genomic DNA. Translation: DAA11826.1.
PIRS52494.
RefSeqNP_010259.1. NM_001180084.1.

3D structure databases

ProteinModelPortalQ12100.
SMRQ12100. Positions 259-613.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid32030. 76 interactions.
DIPDIP-6483N.
IntActQ12100. 19 interactions.
MINTMINT-8285168.
STRING4932.YDL025C.

Proteomic databases

MaxQBQ12100.
PaxDbQ12100.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYDL025C; YDL025C; YDL025C.
GeneID851536.
KEGGsce:YDL025C.

Organism-specific databases

CYGDYDL025c.
SGDS000002183. RTK1.

Phylogenomic databases

eggNOGCOG0515.
GeneTreeENSGT00550000075309.
OMAVARTHLP.
OrthoDBEOG7008J9.

Enzyme and pathway databases

BioCycYEAST:G3O-29452-MONOMER.

Gene expression databases

GenevestigatorQ12100.

Family and domain databases

InterProIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamPF00069. Pkinase. 1 hit.
[Graphical view]
SUPFAMSSF56112. SSF56112. 1 hit.
PROSITEPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio968935.

Entry information

Entry nameRTK1_YEAST
AccessionPrimary (citable) accession number: Q12100
Secondary accession number(s): D6VRW6
Entry history
Integrated into UniProtKB/Swiss-Prot: September 5, 2006
Last sequence update: November 1, 1996
Last modified: May 14, 2014
This is version 112 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome IV

Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families