Probable serine/threonine-protein kinase RTK1 - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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Q12100 (RTK1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 103. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Probable serine/threonine-protein kinase RTK1
EC=2.7.11.1

Alternative name(s):
Ribosome biogenesis and tRNA synthetase-associated kinase 1

Gene names

Name:	RTK1
Ordered Locus Names:	YDL025C
ORF Names:	D2810

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length	620 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Probable serine/threonine-protein kinase that may be involved in ribosome biogenesis. Ref.4
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Subunit structure	Interacts with ribosome biogenesis factors ARC1, CKA2 and GUS1.
Induction	By the alkylating agent methyl methanesulfonate (MMS). Ref.5
Miscellaneous	Present with 861 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Contains 1 protein kinase domain.

Ontologies

Keywords
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW protein serine/threonine kinase activity Inferred from sequence or structural similarity PubMed 20404084. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 620

620

Probable serine/threonine-protein kinase RTK1

PRO_0000248408

Regions

Domain

302 – 575

274

Protein kinase

Nucleotide binding

308 – 316

ATP By similarity

Sites

Active site

430

Proton acceptor By similarity

Binding site

330

ATP By similarity

Amino acid modifications

Modified residue

Phosphoserine Ref.8

Modified residue

Phosphoserine Ref.8

Modified residue

Phosphoserine Ref.8

Modified residue

Phosphoserine Ref.8

Modified residue

Phosphothreonine Ref.8 Ref.9

Modified residue

Phosphoserine Ref.8 Ref.9

Modified residue

Phosphoserine Ref.8 Ref.9

Modified residue

104

Phosphoserine Ref.9

Modified residue

106

Phosphothreonine Ref.9

Modified residue

216

Phosphoserine Ref.9

Modified residue

224

Phosphoserine Ref.6

Modified residue

516

Phosphoserine Ref.9

Sequences

Sequence

Length

Mass (Da)

Tools

Q12100 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: AD6160F19F3B75CF
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FASTA

620

69,615

        10         20         30         40         50         60 
MVKETPLHSS SSTSLSSLFR PTKLKNLSAK IFNGGGNQSY SKTDDVSRSS SRSSKKNTDS 

        70         80         90        100        110        120 
DQEDQIKYNK PNDRRSTIGK SPQGNGALSK ESHVVASSTL TGISPTSAKK APIDYSPSRP 

       130        140        150        160        170        180 
LPNNHNPVRT GHTVPHLPHS IHNPINYIHQ GSKDAFHHPH PVRSTAHSNI STVSSAKSDT 

       190        200        210        220        230        240 
PSSNLSYQAH MHPVEILQKQ IEDKHFMDSQ ASTPGSVELQ HNSSSGSDDT SSRKKKSLRL 

       250        260        270        280        290        300 
TRFFKKIHND YHDNHHHHHH HNRGSTPTKP KLNLNTNENI VESNGKALYE TDNPVELLEK 

       310        320        330        340        350        360 
YGIPGRKLGE GASGSVSVVE RTDGKLFACK MFRKPHLNNE GTNQSQLANY SKKVTTEFCI 

       370        380        390        400        410        420 
GSTLHHENIV ETLDMLTEGD TYLLVMEYAP YDFFNLVMSN LMTQDEVNCY FKQLCHGVNY 

       430        440        450        460        470        480 
LHSMGLAHRD LKLDNCVVTK DGILKLIDFG SAVVFQYPYE DTIVKSHGIV GSDPYLAPEL 

       490        500        510        520        530        540 
LKQTSYDPRV ADVWSIAIIF YCMVLKRFPW KAPKKSFNSF RLFTEEPEDE DDIVRGPNKI 

       550        560        570        580        590        600 
LRLLPRHSRT IIGRMLALEP KQRVLMNDVV KDDWLVSVPS CEVDPTSGDL VEKPKNHKHH 

       610        620 
LVTEEELNEL TKQHGNKDSN

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome IV." Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T. Zaccaria P. Nature 387:75-78(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. LaBaer J. Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	"The protein kinases of budding yeast: six score and more." Hunter T., Plowman G.D. Trends Biochem. Sci. 22:18-22(1997) [PubMed] [Europe PMC] [Abstract] Cited for: PREDICTION OF FUNCTION.
[5]	"Global response of Saccharomyces cerevisiae to an alkylating agent." Jelinsky S.A., Samson L.D. Proc. Natl. Acad. Sci. U.S.A. 96:1486-1491(1999) [PubMed] [Europe PMC] [Abstract] Cited for: INDUCTION.
[6]	"A proteomics approach to understanding protein ubiquitination." Peng J., Schwartz D., Elias J.E., Thoreen C.C., Cheng D., Marsischky G., Roelofs J., Finley D., Gygi S.P. Nat. Biotechnol. 21:921-926(2003) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-224, MASS SPECTROMETRY. Strain: SUB592.
[7]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[8]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-9; SER-11; SER-12; SER-14; THR-58; SER-60 AND SER-81, MASS SPECTROMETRY.
[9]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-58; SER-60; SER-81; SER-104; THR-106; SER-216 AND SER-516, MASS SPECTROMETRY.
[10]	"A global protein kinase and phosphatase interaction network in yeast." Breitkreutz A., Choi H., Sharom J.R., Boucher L., Neduva V., Larsen B., Lin Z.Y., Breitkreutz B.J., Stark C., Liu G., Ahn J., Dewar-Darch D., Reguly T., Tang X., Almeida R., Qin Z.S., Pawson T., Gingras A.C., Nesvizhskii A.I., Tyers M. Science 328:1043-1046(2010) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION [LARGE SCALE ANALYSIS] WITH ARC1; CKA2 AND GUS1.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z74073 Genomic DNA. Translation: CAA98584.1. Z48432 Genomic DNA. Translation: CAA88334.1. AY692716 Genomic DNA. Translation: AAT92735.1. BK006938 Genomic DNA. Translation: DAA11826.1.
PIR	S52494.
RefSeq	NP_010259.1. NM_001180084.1.
3D structure databases
HSSP	HSSP built from PDB template 1ZLT based on UniProtKB O14757.
ProteinModelPortal	Q12100.
SMR	Q12100. Positions 259-613.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-6483N.
IntAct	Q12100. 22 interactions.
MINT	MINT-687326.
STRING	4932.YDL025C.
Proteomic databases
PaxDb	Q12100.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YDL025C; YDL025C; YDL025C.
GeneID	851536.
KEGG	sce:YDL025C.
Organism-specific databases
CYGD	YDL025c.
SGD	S000002183. RTK1.
Phylogenomic databases
eggNOG	COG0515.
GeneTree	ENSGT00550000075309.
OMA	TNENIVE.
OrthoDB	EOG4K9FN3.
Gene expression databases
Genevestigator	Q12100.
GermOnline	YDL025C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR008271. Ser/Thr_kinase_AS. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
SUPFAM	SSF56112. Kinase_like. 1 hit.
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	968935.

Entry information

Entry name

RTK1_YEAST

Accession

Primary (citable) accession number: Q12100
Secondary accession number(s): D6VRW6

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 5, 2006
Last sequence update:	November 1, 1996
Last modified:	May 1, 2013
This is version 103 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome IV

Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Amino acid modifications

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents