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Q12100

- RTK1_YEAST

UniProt

Q12100 - RTK1_YEAST

Protein

Probable serine/threonine-protein kinase RTK1

Gene

RTK1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 113 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Probable serine/threonine-protein kinase that may be involved in ribosome biogenesis.

    Catalytic activityi

    ATP + a protein = ADP + a phosphoprotein.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei330 – 3301ATPPROSITE-ProRule annotation
    Active sitei430 – 4301Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi308 – 3169ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. protein kinase activity Source: SGD
    3. protein serine/threonine kinase activity Source: SGD

    Keywords - Molecular functioni

    Kinase, Serine/threonine-protein kinase, Transferase

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciYEAST:G3O-29452-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Probable serine/threonine-protein kinase RTK1 (EC:2.7.11.1)
    Alternative name(s):
    Ribosome biogenesis and tRNA synthetase-associated kinase 1
    Gene namesi
    Name:RTK1
    Ordered Locus Names:YDL025C
    ORF Names:D2810
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome IV

    Organism-specific databases

    CYGDiYDL025c.
    SGDiS000002183. RTK1.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 620620Probable serine/threonine-protein kinase RTK1PRO_0000248408Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei58 – 581Phosphothreonine1 Publication
    Modified residuei60 – 601Phosphoserine1 Publication
    Modified residuei216 – 2161Phosphoserine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiQ12100.
    PaxDbiQ12100.

    Expressioni

    Inductioni

    By the alkylating agent methyl methanesulfonate (MMS).1 Publication

    Gene expression databases

    GenevestigatoriQ12100.

    Interactioni

    Subunit structurei

    Interacts with ribosome biogenesis factors ARC1, CKA2 and GUS1.1 Publication

    Protein-protein interaction databases

    BioGridi32030. 76 interactions.
    DIPiDIP-6483N.
    IntActiQ12100. 19 interactions.
    MINTiMINT-8285168.
    STRINGi4932.YDL025C.

    Structurei

    3D structure databases

    ProteinModelPortaliQ12100.
    SMRiQ12100. Positions 259-613.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini302 – 575274Protein kinasePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.PROSITE-ProRule annotation
    Contains 1 protein kinase domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0515.
    GeneTreeiENSGT00550000075309.
    OMAiVARTHLP.
    OrthoDBiEOG7008J9.

    Family and domain databases

    InterProiIPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view]
    PfamiPF00069. Pkinase. 1 hit.
    [Graphical view]
    SUPFAMiSSF56112. SSF56112. 1 hit.
    PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q12100-1 [UniParc]FASTAAdd to Basket

    « Hide

    MVKETPLHSS SSTSLSSLFR PTKLKNLSAK IFNGGGNQSY SKTDDVSRSS    50
    SRSSKKNTDS DQEDQIKYNK PNDRRSTIGK SPQGNGALSK ESHVVASSTL 100
    TGISPTSAKK APIDYSPSRP LPNNHNPVRT GHTVPHLPHS IHNPINYIHQ 150
    GSKDAFHHPH PVRSTAHSNI STVSSAKSDT PSSNLSYQAH MHPVEILQKQ 200
    IEDKHFMDSQ ASTPGSVELQ HNSSSGSDDT SSRKKKSLRL TRFFKKIHND 250
    YHDNHHHHHH HNRGSTPTKP KLNLNTNENI VESNGKALYE TDNPVELLEK 300
    YGIPGRKLGE GASGSVSVVE RTDGKLFACK MFRKPHLNNE GTNQSQLANY 350
    SKKVTTEFCI GSTLHHENIV ETLDMLTEGD TYLLVMEYAP YDFFNLVMSN 400
    LMTQDEVNCY FKQLCHGVNY LHSMGLAHRD LKLDNCVVTK DGILKLIDFG 450
    SAVVFQYPYE DTIVKSHGIV GSDPYLAPEL LKQTSYDPRV ADVWSIAIIF 500
    YCMVLKRFPW KAPKKSFNSF RLFTEEPEDE DDIVRGPNKI LRLLPRHSRT 550
    IIGRMLALEP KQRVLMNDVV KDDWLVSVPS CEVDPTSGDL VEKPKNHKHH 600
    LVTEEELNEL TKQHGNKDSN 620
    Length:620
    Mass (Da):69,615
    Last modified:November 1, 1996 - v1
    Checksum:iAD6160F19F3B75CF
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z74073 Genomic DNA. Translation: CAA98584.1.
    Z48432 Genomic DNA. Translation: CAA88334.1.
    AY692716 Genomic DNA. Translation: AAT92735.1.
    BK006938 Genomic DNA. Translation: DAA11826.1.
    PIRiS52494.
    RefSeqiNP_010259.1. NM_001180084.1.

    Genome annotation databases

    EnsemblFungiiYDL025C; YDL025C; YDL025C.
    GeneIDi851536.
    KEGGisce:YDL025C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z74073 Genomic DNA. Translation: CAA98584.1 .
    Z48432 Genomic DNA. Translation: CAA88334.1 .
    AY692716 Genomic DNA. Translation: AAT92735.1 .
    BK006938 Genomic DNA. Translation: DAA11826.1 .
    PIRi S52494.
    RefSeqi NP_010259.1. NM_001180084.1.

    3D structure databases

    ProteinModelPortali Q12100.
    SMRi Q12100. Positions 259-613.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 32030. 76 interactions.
    DIPi DIP-6483N.
    IntActi Q12100. 19 interactions.
    MINTi MINT-8285168.
    STRINGi 4932.YDL025C.

    Proteomic databases

    MaxQBi Q12100.
    PaxDbi Q12100.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YDL025C ; YDL025C ; YDL025C .
    GeneIDi 851536.
    KEGGi sce:YDL025C.

    Organism-specific databases

    CYGDi YDL025c.
    SGDi S000002183. RTK1.

    Phylogenomic databases

    eggNOGi COG0515.
    GeneTreei ENSGT00550000075309.
    OMAi VARTHLP.
    OrthoDBi EOG7008J9.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-29452-MONOMER.

    Miscellaneous databases

    NextBioi 968935.

    Gene expression databases

    Genevestigatori Q12100.

    Family and domain databases

    InterProi IPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view ]
    Pfami PF00069. Pkinase. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56112. SSF56112. 1 hit.
    PROSITEi PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV."
      Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T.
      , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P.
      Nature 387:75-78(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    3. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. "The protein kinases of budding yeast: six score and more."
      Hunter T., Plowman G.D.
      Trends Biochem. Sci. 22:18-22(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: PREDICTION OF FUNCTION.
    5. "Global response of Saccharomyces cerevisiae to an alkylating agent."
      Jelinsky S.A., Samson L.D.
      Proc. Natl. Acad. Sci. U.S.A. 96:1486-1491(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION.
    6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    7. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-58; SER-60 AND SER-216, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    8. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    9. Cited for: INTERACTION [LARGE SCALE ANALYSIS] WITH ARC1; CKA2 AND GUS1.
    10. "Sites of ubiquitin attachment in Saccharomyces cerevisiae."
      Starita L.M., Lo R.S., Eng J.K., von Haller P.D., Fields S.
      Proteomics 12:236-240(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiRTK1_YEAST
    AccessioniPrimary (citable) accession number: Q12100
    Secondary accession number(s): D6VRW6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 5, 2006
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 113 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 861 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome IV
      Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

    External Data

    Dasty 3