Q12094 (TSR3_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribosome biogenesis protein TSR3

Alternative name(s):
20S rRNA accumulation protein 3

Gene names

Name:	TSR3
Ordered Locus Names:	YOR006C
ORF Names:	UND313

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	313 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Ribosome biogenesis protein required for processing 35S pre-rRNA at site D. Ref.7
Subcellular location	Cytoplasm. Nucleus Ref.4.
Miscellaneous	Present with 3610 molecules/cell in log phase SD medium. Ref.5
Sequence similarities	Belongs to the TSR3 family.

Ontologies

Keywords
Biological process	Ribosome biogenesis rRNA processing
Cellular component	Cytoplasm Nucleus
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	maturation of SSU-rRNA Inferred from mutant phenotype Ref.7. Source: SGD
Cellular component	cytoplasm Inferred from direct assay Ref.4. Source: SGD nucleus Inferred from direct assay Ref.4. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
itself		1	EBI-29255,EBI-29255

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 313	313	Ribosome biogenesis protein TSR3		PRO_0000094426
Amino acid modifications
	Modified residue	286	1	Phosphoserine Ref.6
	Modified residue	289	1	Phosphoserine Ref.6

Sequences

	Sequence		Length	Mass (Da)
	Q12094 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 6DF4703EA7252910	FASTA	313	35,686
	10 20 30 40 50 60 MGKGKNKMHE PKNGRPQRGA NGHSSRQNHR RMEMKYDNSE KMKFPVKLAM WDFDHCDPKR 70 80 90 100 110 120 CSGKKLERLG LIKSLRVGQK FQGIVVSPNG KGVVCPDDLE IVEQHGASVV ECSWARLEEV 130 140 150 160 170 180 PFNKIGGKHE RLLPYLVAAN QVNYGRPWRL NCVEALAACF AIVGRMDWAS ELLSHFSWGM 190 200 210 220 230 240 GFLELNKELL EIYQQCTDCD SVKRAEEEWL QKLEKETQER KSRAKEEDIW MMGNINRRGN 250 260 270 280 290 300 GSQSDTSESE ENSEQSDLEG NNQCIEYDSL GNAIRIDNMK SREAQSEESE DEESGSKENG 310 EPLSYDPLGN LIR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The sequence of a 30 kb fragment on the left arm of chromosome XV from Saccharomyces cerevisiae reveals 15 open reading frames, five of which correspond to previously identified genes." Sterky F., Holmberg A., Pettersson B., Uhlen M. Yeast 12:1091-1095(1996) [PubMed: 8896276] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XV." Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D. , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K. Nature 387:98-102(1997) [PubMed: 9169874] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed: 17563356] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-286 AND SER-289, MASS SPECTROMETRY.
[7]	"Rational extension of the ribosome biogenesis pathway using network-guided genetics." Li Z., Lee I., Moradi E., Hung N.J., Johnson A.W., Marcotte E.M. PLoS Biol. 7:E1000213-E1000213(2009) [PubMed: 19806183] [Abstract] Cited for: FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U43491 Genomic DNA. Translation: AAC49486.1. Z74914 Genomic DNA. Translation: CAA99194.1. BK006948 Genomic DNA. Translation: DAA10788.1.
PIR	S61990.
RefSeq	NP_014648.1. NM_001183425.1.
3D structure databases
ProteinModelPortal	Q12094.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-1251N.
IntAct	Q12094. 7 interactions.
MINT	MINT-395199.
STRING	Q12094.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YOR006C; YOR006C; YOR006C.
GeneID	854167.
KEGG	sce:YOR006C.
NMPDR	fig|4932.3.peg.5743.
Organism-specific databases
CYGD	YOR006c.
SGD	S000005532. YOR006C.
Phylogenomic databases
eggNOG	fuNOG07953.
GeneTree	EFGT00050000003113.
HOGENOM	HBG527152.
OMA	VGQKFQG.
OrthoDB	EOG4FR41Q.
Gene expression databases
ArrayExpress	Q12094.
Genevestigator	Q12094.
GermOnline	YOR006C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR007177. DUF367. IPR022968. Rbsm_biogenesis. IPR007209. RNaseL-inhib_metal-bd_dom. [Graphical view]
KO	K09140.
PANTHER	PTHR20426. PTHR20426. 1 hit.
Pfam	PF04034. DUF367. 1 hit. PF04068. RLI. 1 hit. [Graphical view]
ProDom	PD016638. DUF367. 1 hit. [Graphical view] [Entries sharing at least one domain]
ProtoNet	Search...
Other
NextBio	975953.

Entry information

Entry name

TSR3_YEAST

Accession

Primary (citable) accession number: Q12094
Secondary accession number(s): D6W272

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1996
Last modified:	December 14, 2011
This is version 78 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program