Q12091 (DAP1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Damage response protein 1

Gene names

Name:	DAP1
Ordered Locus Names:	YPL170W
ORF Names:	P2515

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	152 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Domain	The cytochrome b5 heme-binding domain lacks the conserved iron-binding His residues at positions 81 and 105 By similarity.
Miscellaneous	Present with 6300 molecules/cell in log phase SD medium. Ref.6
Sequence similarities	Belongs to the cytochrome b5 family. MAPR subfamily. Contains 1 cytochrome b5 heme-binding domain.

Ontologies

Keywords
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	regulation of ergosterol biosynthetic process Inferred from mutant phenotype. Source: SGD
Cellular component	endosome Inferred from direct assay. Source: SGD
Molecular function	heme binding Inferred from direct assay. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
AGP1	P25376	1	EBI-5554,EBI-2357
ALO1	P54783	1	EBI-5554,EBI-2519
PMP2	P40975	1	EBI-5554,EBI-2043041
PMP3	P87284	1	EBI-5554,EBI-13555
RTN1	Q04947	1	EBI-5554,EBI-38020
YHR045W	P38775	1	EBI-5554,EBI-24520

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 152	152	Damage response protein 1		PRO_0000121747
Regions
	Domain	46 – 145	100	Cytochrome b5 heme-binding

Sequences

	Sequence		Length	Mass (Da)
	Q12091 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 3E3CAC2403C3246F	FASTA	152	16,758
	10 20 30 40 50 60 MSFIKNLLFG GVKTSEDPTG LTGNGASNTN DSNKGSEPVV AGNFFPRTLS KFNGHDDEKI 70 80 90 100 110 120 FIAIRGKVYD CTRGRQFYGP SGPYTNFAGH DASRGLALNS FDLDVIKDWD QPIDPLDDLT 130 140 150 KEQIDALDEW QEHFENKYPC IGTLIPEPGV NV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The sequence of 55 kb on the left arm of yeast chromosome XVI identifies a small nuclear RNA, a new putative protein kinase and two new putative regulators." Purnelle B., Coster F., Goffeau A. Yeast 12:1483-1492(1996) [PubMed: 8948103] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI." Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M. , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J. Nature 387:103-105(1997) [PubMed: 9169875] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[5]	"Saccharomyces cerevisiae Dap1p, a novel DNA damage response protein related to the mammalian membrane-associated progesterone receptor." Hand R.A., Jia N., Bard M., Craven R.J. Eukaryot. Cell 2:306-317(2003) [PubMed: 12684380] [Abstract] Cited for: CHARACTERIZATION.
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X96770 Genomic DNA. Translation: CAA65551.1. Z73526 Genomic DNA. Translation: CAA97876.1. AY558521 Genomic DNA. Translation: AAS56847.1. BK006949 Genomic DNA. Translation: DAA11264.1.
PIR	S65181.
RefSeq	NP_015155.1. NM_001183984.1.
3D structure databases
ProteinModelPortal	Q12091.
SMR	Q12091. Positions 39-146.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-5226N.
IntAct	Q12091. 8 interactions.
MINT	MINT-512159.
STRING	Q12091.
Proteomic databases
PeptideAtlas	Q12091.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YPL170W; YPL170W; YPL170W.
GeneID	855933.
KEGG	sce:YPL170W.
NMPDR	fig|4932.3.peg.6284.
Organism-specific databases
CYGD	YPL170w.
SGD	S000006091. DAP1.
Phylogenomic databases
eggNOG	fuNOG11622.
GeneTree	EFGT00050000004679.
HOGENOM	HBG715794.
OMA	DEKIFIA.
OrthoDB	EOG4KD9WR.
Gene expression databases
ArrayExpress	Q12091.
Genevestigator	Q12091.
GermOnline	YPL170W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR001199. Cyt_B5. [Graphical view]
Gene3D	G3DSA:3.10.120.10. Cyt_B5. 1 hit.
Pfam	PF00173. Cyt-b5. 1 hit. [Graphical view]
SUPFAM	SSF55856. Cyt_B5. 1 hit.
PROSITE	PS00191. CYTOCHROME_B5_1. False negative. PS50255. CYTOCHROME_B5_2. False negative. [Graphical view]
ProtoNet	Search...
Other
NextBio	980678.

Entry information

Entry name

DAP1_YEAST

Accession

Primary (citable) accession number: Q12091
Secondary accession number(s): D6W3J8

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	November 1, 1996
Last modified:	December 14, 2011
This is version 97 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program