Q12089 (AEP3_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
ATPase expression protein 3

Gene names

Name:	AEP3
Ordered Locus Names:	YPL005W
ORF Names:	YP8132.08

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	606 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Required for respiration. Stabilizes the mitochondrial bicistronic mRNA encoding ATP6 and ATP8, 2 subunits of the proton-translocating ATP synthase. Ref.6
Subcellular location	Mitochondrion inner membrane; Peripheral membrane protein; Matrix side Ref.4 Ref.6.
Miscellaneous	Present with 1760 molecules/cell in log phase SD medium. Ref.5
Sequence similarities	Contains 3 PPR (pentatricopeptide) repeats.

Ontologies

Keywords
Cellular component	Membrane Mitochondrion Mitochondrion inner membrane
Domain	Repeat
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	mRNA metabolic process Inferred from mutant phenotype Ref.6. Source: SGD
Cellular component	extrinsic to membrane Inferred from direct assay Ref.6. Source: SGD mitochondrial inner membrane Inferred from direct assay Ref.6. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 606	606	ATPase expression protein 3		PRO_0000064466
Regions
	Repeat	220 – 254	35	PPR 1
	Repeat	397 – 432	36	PPR 2
	Repeat	433 – 467	35	PPR 3

Sequences

	Sequence		Length	Mass (Da)
	Q12089 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: B7F93D1FD22677F4	FASTA	606	70,310
	10 20 30 40 50 60 MNTLRCLTQA LSKSGREAPK LYQKVIFPGL FREGIPIANV KKVDEKIIDS PTSTSVNGEA 70 80 90 100 110 120 KKIVRHGVKY EREQVKEYLS SLPTLTLSRK QIRDDYDEER AKRMYMFSKQ TNSSNKFQKL 130 140 150 160 170 180 LTAKSQEFTR ELLTLLIDCT SNEKNSGPER FTRKFLKFSN DEIPPLPDFS KNPQLFENYI 190 200 210 220 230 240 GILSHTKFNF RSSSKLNGIV RKMLRHLLHP TNKTTLPLRS AQVYNDSIYF FSEHFDFASC 250 260 270 280 290 300 REIFAQMKAE GTKPNTITFN LLLRNVVKNS HIRKTKHPDD EVLFYLRSMR NHGVFADVIT 310 320 330 340 350 360 WTTCYNFLRD EVSRQLYIVQ MGEHLGNFNV NFVYTVLRNG DYRAEDCLKV LAANSLPISR 370 380 390 400 410 420 KTFYLCIERL LNEEQLETAS KLLDYGFQHL KSNFKLDSEA MNHFMRVFAN KGRSDLAFLC 430 440 450 460 470 480 YNTCRKIYKI KPDSQTFEML FKALVRNGNT KNFGAVLQYI KDLKVSEGFG LRTSYWRTKA 490 500 510 520 530 540 DSIFKFGSPN TLSEKSIEKA RKLLGNLIAS EGEFSWKIWK ESDSSQKKIL RFLGCIPTTL 550 560 570 580 590 600 RCTNTAQDHQ KPTNLPSNIS QKKREYRNRV KAIATKAALE KRMAYIKDND VAFKKELVKR RIVGEV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI." Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M. , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J. Nature 387:103-105(1997) [PubMed: 9169875] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]	"Aep3p stabilizes the mitochondrial bicistronic mRNA encoding subunits 6 and 8 of the H+-translocating ATP synthase of Saccharomyces cerevisiae." Ellis T.P., Helfenbein K.G., Tzagoloff A., Dieckmann C.L. J. Biol. Chem. 279:15728-15733(2004) [PubMed: 14742425] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z48483 Genomic DNA. Translation: CAA88381.1. Z71255 Genomic DNA. Translation: CAA95036.1. U33335 Genomic DNA. Translation: AAB68100.1. AY692659 Genomic DNA. Translation: AAT92678.1. BK006949 Genomic DNA. Translation: DAA11423.1.
PIR	S52526.
RefSeq	NP_015320.1. NM_001183819.1.
3D structure databases
ProteinModelPortal	Q12089.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-4045N.
MINT	MINT-474781.
STRING	Q12089.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YPL005W; YPL005W; YPL005W.
GeneID	856102.
KEGG	sce:YPL005W.
NMPDR	fig|4932.3.peg.6454.
Organism-specific databases
CYGD	YPL005w.
SGD	S000005926. AEP3.
Phylogenomic databases
eggNOG	fuNOG08513.
HOGENOM	HBG203940.
OMA	FATCREL.
OrthoDB	EOG447K34.
Gene expression databases
ArrayExpress	Q12089.
Genevestigator	Q12089.
GermOnline	YPL005W. Saccharomyces cerevisiae.
Family and domain databases
PROSITE	PS51375. PPR. False negative. [Graphical view]
ProtoNet	Search...
Other
NextBio	981152.

Entry information

Entry name

AEP3_YEAST

Accession

Primary (citable) accession number: Q12089
Secondary accession number(s): D6W407

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 25, 2005
Last sequence update:	November 1, 1996
Last modified:	December 14, 2011
This is version 72 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program