Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Putative uridine kinase DAS2

Gene

DAS2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Putative uridine kinase identified in a screen for mutants with increased levels of rDNA transcription.1 Publication

Miscellaneous

Present with 4280 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

ATP + uridine = ADP + UMP.
ATP + cytidine = ADP + CMP.

Pathwayi: CTP biosynthesis via salvage pathway

This protein is involved in step 1 of the subpathway that synthesizes CTP from cytidine.
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Putative uridine kinase DAS2 (DAS2), Uridine kinase (URK1)
  2. no protein annotated in this organism
  3. no protein annotated in this organism
This subpathway is part of the pathway CTP biosynthesis via salvage pathway, which is itself part of Pyrimidine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes CTP from cytidine, the pathway CTP biosynthesis via salvage pathway and in Pyrimidine metabolism.

Pathwayi: UMP biosynthesis via salvage pathway

This protein is involved in step 1 of the subpathway that synthesizes UMP from uridine.
Proteins known to be involved in this subpathway in this organism are:
  1. Putative uridine kinase DAS2 (DAS2), Uridine kinase (URK1)
This subpathway is part of the pathway UMP biosynthesis via salvage pathway, which is itself part of Pyrimidine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes UMP from uridine, the pathway UMP biosynthesis via salvage pathway and in Pyrimidine metabolism.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi17 – 24ATPSequence analysis8

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Transferase
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-29637-MONOMER
UniPathwayiUPA00574; UER00637
UPA00579; UER00640

Names & Taxonomyi

Protein namesi
Recommended name:
Putative uridine kinase DAS2 (EC:2.7.1.48)
Alternative name(s):
DST1-delta 6-azauracil sensitivity protein 2
Regulator of rDNA transcription 3
Gene namesi
Name:DAS2
Synonyms:RRT3
Ordered Locus Names:YDR020C
ORF Names:PZE232
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

EuPathDBiFungiDB:YDR020C
SGDiS000002427 DAS2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002424891 – 232Putative uridine kinase DAS2Add BLAST232

Proteomic databases

MaxQBiQ12084
PaxDbiQ12084
PRIDEiQ12084

PTM databases

iPTMnetiQ12084

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
URK1P275154EBI-35824,EBI-20151

Protein-protein interaction databases

BioGridi32070, 79 interactors
DIPiDIP-1721N
IntActiQ12084, 1 interactor
MINTiQ12084
STRINGi4932.YDR020C

Structurei

3D structure databases

ProteinModelPortaliQ12084
SMRiQ12084
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the uridine kinase family.Curated

Phylogenomic databases

InParanoidiQ12084
KOiK00876
OMAiFADVIMP
OrthoDBiEOG092C5CLC

Family and domain databases

InterProiView protein in InterPro
IPR027417 P-loop_NTPase
IPR006083 PRK/URK
PfamiView protein in Pfam
PF00485 PRK, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit

Sequencei

Sequence statusi: Complete.

Q12084-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDRKAVEEKR IVISIGGGHA TGVGAIALDL QNTFKSLYNS INIRVINLDN
60 70 80 90 100
MIEGNIKSYN NNDYDFDNIL NLVYEKHAVT SQNDMIQHDY EDPIDLIIVC
110 120 130 140 150
GCYALYDKRI NEISQLKVFL DSDADKRLIS LIKKKNVGSN EQLAQLITEY
160 170 180 190 200
MDHLRPEMQQ YIEPTRTFAD LIIPSTNENL GRAVLVDGIV KAIEDTKSQI
210 220 230
EGNNTNNKIR PRLWDFEAET MDLEKDRYYD LS
Length:232
Mass (Da):26,595
Last modified:November 1, 1996 - v1
Checksum:i4171B063EE750613
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X95966 Genomic DNA Translation: CAA65212.1
Z49770 Genomic DNA Translation: CAA89845.1
Z74316 Genomic DNA Translation: CAA98841.1
AY558571 Genomic DNA Translation: AAS56897.1
BK006938 Genomic DNA Translation: DAA11866.1
PIRiS54643
RefSeqiNP_010303.3, NM_001180328.3

Genome annotation databases

EnsemblFungiiYDR020C; YDR020C; YDR020C
GeneIDi851583
KEGGisce:YDR020C

Similar proteinsi

Entry informationi

Entry nameiDAS2_YEAST
AccessioniPrimary (citable) accession number: Q12084
Secondary accession number(s): D6VS06
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 27, 2006
Last sequence update: November 1, 1996
Last modified: April 25, 2018
This is version 148 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health