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Protein

Putative uridine kinase DAS2

Gene

DAS2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Putative uridine kinase identified in a screen for mutants with increased levels of rDNA transcription.1 Publication

Catalytic activityi

ATP + uridine = ADP + UMP.
ATP + cytidine = ADP + CMP.

Pathwayi: CTP biosynthesis via salvage pathway

This protein is involved in step 1 of the subpathway that synthesizes CTP from cytidine.
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Putative uridine kinase DAS2 (DAS2), Uridine kinase (URK1)
  2. no protein annotated in this organism
  3. no protein annotated in this organism
This subpathway is part of the pathway CTP biosynthesis via salvage pathway, which is itself part of Pyrimidine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes CTP from cytidine, the pathway CTP biosynthesis via salvage pathway and in Pyrimidine metabolism.

Pathwayi: UMP biosynthesis via salvage pathway

This protein is involved in step 1 of the subpathway that synthesizes UMP from uridine.
Proteins known to be involved in this subpathway in this organism are:
  1. Putative uridine kinase DAS2 (DAS2), Uridine kinase (URK1)
This subpathway is part of the pathway UMP biosynthesis via salvage pathway, which is itself part of Pyrimidine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes UMP from uridine, the pathway UMP biosynthesis via salvage pathway and in Pyrimidine metabolism.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi17 – 24ATPSequence analysis8

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-29637-MONOMER.
UniPathwayiUPA00574; UER00637.
UPA00579; UER00640.

Names & Taxonomyi

Protein namesi
Recommended name:
Putative uridine kinase DAS2 (EC:2.7.1.48)
Alternative name(s):
DST1-delta 6-azauracil sensitivity protein 2
Regulator of rDNA transcription 3
Gene namesi
Name:DAS2
Synonyms:RRT3
Ordered Locus Names:YDR020C
ORF Names:PZE232
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

EuPathDBiFungiDB:YDR020C.
SGDiS000002427. DAS2.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002424891 – 232Putative uridine kinase DAS2Add BLAST232

Proteomic databases

MaxQBiQ12084.
PRIDEiQ12084.

PTM databases

iPTMnetiQ12084.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
URK1P275155EBI-35824,EBI-20151

Protein-protein interaction databases

BioGridi32070. 10 interactors.
DIPiDIP-1721N.
IntActiQ12084. 1 interactor.
MINTiMINT-407001.

Structurei

3D structure databases

ProteinModelPortaliQ12084.
SMRiQ12084.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the uridine kinase family.Curated

Phylogenomic databases

InParanoidiQ12084.
OMAiMDHLRPE.
OrthoDBiEOG092C5CLC.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR006083. PRK/URK.
[Graphical view]
PfamiPF00485. PRK. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.

Sequencei

Sequence statusi: Complete.

Q12084-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDRKAVEEKR IVISIGGGHA TGVGAIALDL QNTFKSLYNS INIRVINLDN
60 70 80 90 100
MIEGNIKSYN NNDYDFDNIL NLVYEKHAVT SQNDMIQHDY EDPIDLIIVC
110 120 130 140 150
GCYALYDKRI NEISQLKVFL DSDADKRLIS LIKKKNVGSN EQLAQLITEY
160 170 180 190 200
MDHLRPEMQQ YIEPTRTFAD LIIPSTNENL GRAVLVDGIV KAIEDTKSQI
210 220 230
EGNNTNNKIR PRLWDFEAET MDLEKDRYYD LS
Length:232
Mass (Da):26,595
Last modified:November 1, 1996 - v1
Checksum:i4171B063EE750613
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X95966 Genomic DNA. Translation: CAA65212.1.
Z49770 Genomic DNA. Translation: CAA89845.1.
Z74316 Genomic DNA. Translation: CAA98841.1.
AY558571 Genomic DNA. Translation: AAS56897.1.
BK006938 Genomic DNA. Translation: DAA11866.1.
PIRiS54643.
RefSeqiNP_010303.3. NM_001180328.3.

Genome annotation databases

EnsemblFungiiYDR020C; YDR020C; YDR020C.
GeneIDi851583.
KEGGisce:YDR020C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X95966 Genomic DNA. Translation: CAA65212.1.
Z49770 Genomic DNA. Translation: CAA89845.1.
Z74316 Genomic DNA. Translation: CAA98841.1.
AY558571 Genomic DNA. Translation: AAS56897.1.
BK006938 Genomic DNA. Translation: DAA11866.1.
PIRiS54643.
RefSeqiNP_010303.3. NM_001180328.3.

3D structure databases

ProteinModelPortaliQ12084.
SMRiQ12084.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi32070. 10 interactors.
DIPiDIP-1721N.
IntActiQ12084. 1 interactor.
MINTiMINT-407001.

PTM databases

iPTMnetiQ12084.

Proteomic databases

MaxQBiQ12084.
PRIDEiQ12084.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYDR020C; YDR020C; YDR020C.
GeneIDi851583.
KEGGisce:YDR020C.

Organism-specific databases

EuPathDBiFungiDB:YDR020C.
SGDiS000002427. DAS2.

Phylogenomic databases

InParanoidiQ12084.
OMAiMDHLRPE.
OrthoDBiEOG092C5CLC.

Enzyme and pathway databases

UniPathwayiUPA00574; UER00637.
UPA00579; UER00640.
BioCyciYEAST:G3O-29637-MONOMER.

Miscellaneous databases

PROiQ12084.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR006083. PRK/URK.
[Graphical view]
PfamiPF00485. PRK. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiDAS2_YEAST
AccessioniPrimary (citable) accession number: Q12084
Secondary accession number(s): D6VS06
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 27, 2006
Last sequence update: November 1, 1996
Last modified: November 2, 2016
This is version 138 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 4280 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.