Q12084 (DAS2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Putative uridine kinase DAS2
EC=2.7.1.48

Alternative name(s):
DST1-delta 6-azauracil sensitivity protein 2
Regulator of rDNA transcription 3

Gene names

Name:	DAS2
Synonyms:	RRT3
Ordered Locus Names:	YDR020C
ORF Names:	PZE232

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	232 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Putative uridine kinase identified in a screen for mutants with increased levels of rDNA transcription. Ref.8
Catalytic activity	ATP + uridine = ADP + UMP. ATP + cytidine = ADP + CMP.
Pathway	Pyrimidine metabolism; CTP biosynthesis via salvage pathway; CTP from cytidine: step 1/3. Pyrimidine metabolism; UMP biosynthesis via salvage pathway; UMP from uridine: step 1/1.
Subcellular location	Cytoplasm. Nucleus Ref.6.
Miscellaneous	Present with 4280 molecules/cell in log phase SD medium. Ref.7
Sequence similarities	Belongs to the uridine kinase family.

Ontologies

Keywords
Cellular component	Cytoplasm Nucleus
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from direct assay Ref.6. Source: SGD nucleus Inferred from direct assay Ref.6. Source: SGD
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW protein binding Inferred from physical interaction. Source: IntAct uridine kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
URK1	P27515	5	EBI-35824,EBI-20151

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 232	232	Putative uridine kinase DAS2		PRO_0000242489
Regions
	Nucleotide binding	17 – 24	8	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	Q12084 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 4171B063EE750613	FASTA	232	26,595
	10 20 30 40 50 60 MDRKAVEEKR IVISIGGGHA TGVGAIALDL QNTFKSLYNS INIRVINLDN MIEGNIKSYN 70 80 90 100 110 120 NNDYDFDNIL NLVYEKHAVT SQNDMIQHDY EDPIDLIIVC GCYALYDKRI NEISQLKVFL 130 140 150 160 170 180 DSDADKRLIS LIKKKNVGSN EQLAQLITEY MDHLRPEMQQ YIEPTRTFAD LIIPSTNENL 190 200 210 220 230 GRAVLVDGIV KAIEDTKSQI EGNNTNNKIR PRLWDFEAET MDLEKDRYYD LS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequencing and analysis of a 35.4 kb region on the right arm of chromosome IV from Saccharomyces cerevisiae reveal 23 open reading frames." Eide L.G., Sander C., Prydz H. Yeast 12:1085-1090(1996) [PubMed: 8896275] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	Erratum Eide L.G., Sander C., Prydz H. Yeast 13:189-189(1997)
[3]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome IV." Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T. , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P. Nature 387:75-78(1997) [PubMed: 9169867] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[5]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[6]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[7]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[8]	"Genetic identification of factors that modulate ribosomal DNA transcription in Saccharomyces cerevisiae." Hontz R.D., Niederer R.O., Johnson J.M., Smith J.S. Genetics 182:105-119(2009) [PubMed: 19270272] [Abstract] Cited for: FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X95966 Genomic DNA. Translation: CAA65212.1. Z49770 Genomic DNA. Translation: CAA89845.1. Z74316 Genomic DNA. Translation: CAA98841.1. AY558571 Genomic DNA. Translation: AAS56897.1. BK006938 Genomic DNA. Translation: DAA11866.1.
PIR	S54643.
RefSeq	NP_010303.1. NM_001180328.1.
3D structure databases
ProteinModelPortal	Q12084.
SMR	Q12084. Positions 8-201.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-1721N.
IntAct	Q12084. 3 interactions.
MINT	MINT-407001.
STRING	Q12084.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YDR020C; YDR020C; YDR020C.
GeneID	851583.
KEGG	sce:YDR020C.
NMPDR	fig|4932.3.peg.1046.
Organism-specific databases
CYGD	YDR020c.
SGD	S000002427. DAS2.
Phylogenomic databases
eggNOG	fuNOG10690.
HOGENOM	HBG203124.
OMA	CGCYALY.
OrthoDB	EOG43V352.
Gene expression databases
ArrayExpress	Q12084.
Genevestigator	Q12084.
GermOnline	YDR020C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR006083. PRK/URK. [Graphical view]
Pfam	PF00485. PRK. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	969052.

Entry information

Entry name

DAS2_YEAST

Accession

Primary (citable) accession number: Q12084
Secondary accession number(s): D6VS06

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 27, 2006
Last sequence update:	November 1, 1996
Last modified:	December 14, 2011
This is version 98 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program