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Q12083

- MLH3_YEAST

UniProt

Q12083 - MLH3_YEAST

Protein

DNA mismatch repair protein MLH3

Gene

MLH3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 99 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Involved in DNA mismatch repair (MMR), correcting insertion-deletion loops (IDLs) resulting from DNA replication, DNA damage or from recombination events between non-identical sequences during meiosis. Component of the MutLbeta heterodimer, which probably forms a ternary complex with the MutSbeta heterodimer that initially recognizes the DNA mismatches. This complex is thought to be responsible for directing the downsteam MMR events, including strand discrimination, excision, and resynthesis. Plays a major role in promoting meiotic crossing-over and is involved in maintaining the genetic stability of simple sequence repeats by correction of frameshift intermediates.3 Publications

    GO - Molecular functioni

    1. ATPase activity Source: SGD
    2. ATP binding Source: InterPro
    3. protein binding Source: IntAct

    GO - Biological processi

    1. ATP catabolic process Source: GOC
    2. meiotic mismatch repair Source: SGD
    3. mismatch repair Source: SGD
    4. reciprocal meiotic recombination Source: SGD

    Keywords - Biological processi

    DNA damage, DNA repair

    Enzyme and pathway databases

    BioCyciYEAST:G3O-34060-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    DNA mismatch repair protein MLH3
    Alternative name(s):
    MutL protein homolog 3
    Gene namesi
    Name:MLH3
    Ordered Locus Names:YPL164C
    ORF Names:P2550
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XVI

    Organism-specific databases

    CYGDiYPL164c.
    SGDiS000006085. MLH3.

    Subcellular locationi

    Nucleus Curated

    GO - Cellular componenti

    1. MutLgamma complex Source: SGD
    2. nucleus Source: SGD

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 715715DNA mismatch repair protein MLH3PRO_0000245570Add
    BLAST

    Proteomic databases

    MaxQBiQ12083.

    Expressioni

    Gene expression databases

    GenevestigatoriQ12083.

    Interactioni

    Subunit structurei

    Heterodimer of MLH1 and MLH3, called MutLbeta, which is involved in correction of a specific subset of IDLs when associated with MutSbeta. Forms a ternary complex with a SGS1-TOP3 heterodimer during meiosis.

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    MLH1P389204EBI-31634,EBI-11003

    Protein-protein interaction databases

    BioGridi36019. 10 interactions.
    DIPiDIP-2414N.
    IntActiQ12083. 3 interactions.
    MINTiMINT-678752.
    STRINGi4932.YPL164C.

    Structurei

    3D structure databases

    ProteinModelPortaliQ12083.
    SMRiQ12083. Positions 26-289.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0323.
    GeneTreeiENSGT00550000074903.
    HOGENOMiHOG000113600.
    KOiK08739.
    OMAiRIRVERY.
    OrthoDBiEOG77Q552.

    Family and domain databases

    Gene3Di3.30.230.10. 1 hit.
    3.30.565.10. 1 hit.
    InterProiIPR003594. HATPase_ATP-bd.
    IPR028830. Mlh3.
    IPR014790. MutL_C.
    IPR020568. Ribosomal_S5_D2-typ_fold.
    IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
    [Graphical view]
    PANTHERiPTHR10073:SF7. PTHR10073:SF7. 1 hit.
    PfamiPF08676. MutL_C. 1 hit.
    [Graphical view]
    SMARTiSM00853. MutL_C. 1 hit.
    [Graphical view]
    SUPFAMiSSF54211. SSF54211. 1 hit.
    SSF55874. SSF55874. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q12083-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSQHIRKLDS DVSERLKSQA CTVSLASAVR EIVQNSVDAH ATTIDVMIDL    50
    PNLSFAVYDD GIGLTRSDLN ILATQNYTSK IRKMNDLVTM KTYGYRGDAL 100
    YSISNVSNLF VCSKKKDYNS AWMRKFPSKS VMLSENTILP IDPFWKICPW 150
    SRTKSGTVVI VEDMLYNLPV RRRILKEEPP FKTFNTIKAD MLQILVMHPM 200
    ISLNVQYTDK LRINTEVLFR SKNITEGLTK HQQMSQVLRN VFGAIIPPDM 250
    LKKVSLKFNE YQIEGIISKM PVGLKDLQFI YINGRRYADS AFQGYVDSLF 300
    QAQDFGEKGM SLLKTKSVGK PYRSHPVFIL DVRCPQTIDD LLQDPAKKIV 350
    KPSHIRTIEP LIVKTIRSFL TFQGYLTPDK SDSSFEIVNC SQKTATLPDS 400
    RIQISKRNQV LNSKMKIARI NSYIGKPAVN GCRINNSTIN YEKIKNIRID 450
    GQKSRLRNKL SSRPYDSGFT EDYDSIGKTI TDFSISRSVL AKYEVINQVD 500
    KKFILIRCLD QSIHNCPLLV LVDQHACDER IRLEELFYSL LTEVVTGTFV 550
    ARDLKDCCIE VDRTEADLFK HYQSEFKKWG IGYETIEGTM ETSLLEIKTL 600
    PEMLTSKYNG DKDYLKMVLL QHAHDLKDFK KLPMDLSHFE NYTSVDKLYW 650
    WKYSSCVPTV FHEILNSKAC RSAVMFGDEL TRQECIILIS KLSRCHNPFE 700
    CAHGRPSMVP IAELK 715
    Length:715
    Mass (Da):82,001
    Last modified:November 1, 1996 - v1
    Checksum:i970FD7F57EB6E3B1
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X96770 Genomic DNA. Translation: CAA65557.1.
    Z73520 Genomic DNA. Translation: CAA97869.1.
    BK006949 Genomic DNA. Translation: DAA11270.1.
    PIRiS65175.
    RefSeqiNP_015161.1. NM_001183978.1.

    Genome annotation databases

    EnsemblFungiiYPL164C; YPL164C; YPL164C.
    GeneIDi855939.
    KEGGisce:YPL164C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X96770 Genomic DNA. Translation: CAA65557.1 .
    Z73520 Genomic DNA. Translation: CAA97869.1 .
    BK006949 Genomic DNA. Translation: DAA11270.1 .
    PIRi S65175.
    RefSeqi NP_015161.1. NM_001183978.1.

    3D structure databases

    ProteinModelPortali Q12083.
    SMRi Q12083. Positions 26-289.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 36019. 10 interactions.
    DIPi DIP-2414N.
    IntActi Q12083. 3 interactions.
    MINTi MINT-678752.
    STRINGi 4932.YPL164C.

    Proteomic databases

    MaxQBi Q12083.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YPL164C ; YPL164C ; YPL164C .
    GeneIDi 855939.
    KEGGi sce:YPL164C.

    Organism-specific databases

    CYGDi YPL164c.
    SGDi S000006085. MLH3.

    Phylogenomic databases

    eggNOGi COG0323.
    GeneTreei ENSGT00550000074903.
    HOGENOMi HOG000113600.
    KOi K08739.
    OMAi RIRVERY.
    OrthoDBi EOG77Q552.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-34060-MONOMER.

    Miscellaneous databases

    NextBioi 980696.
    PROi Q12083.

    Gene expression databases

    Genevestigatori Q12083.

    Family and domain databases

    Gene3Di 3.30.230.10. 1 hit.
    3.30.565.10. 1 hit.
    InterProi IPR003594. HATPase_ATP-bd.
    IPR028830. Mlh3.
    IPR014790. MutL_C.
    IPR020568. Ribosomal_S5_D2-typ_fold.
    IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
    [Graphical view ]
    PANTHERi PTHR10073:SF7. PTHR10073:SF7. 1 hit.
    Pfami PF08676. MutL_C. 1 hit.
    [Graphical view ]
    SMARTi SM00853. MutL_C. 1 hit.
    [Graphical view ]
    SUPFAMi SSF54211. SSF54211. 1 hit.
    SSF55874. SSF55874. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "The sequence of 55 kb on the left arm of yeast chromosome XVI identifies a small nuclear RNA, a new putative protein kinase and two new putative regulators."
      Purnelle B., Coster F., Goffeau A.
      Yeast 12:1483-1492(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204511 / S288c / AB972.
    2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI."
      Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M.
      , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J.
      Nature 387:103-105(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. "The Saccharomyces cerevisiae MLH3 gene functions in MSH3-dependent suppression of frameshift mutations."
      Flores-Rozas H., Kolodner R.D.
      Proc. Natl. Acad. Sci. U.S.A. 95:12404-12409(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH MLH1.
    5. "Functional specificity of MutL homologs in yeast: evidence for three Mlh1-based heterocomplexes with distinct roles during meiosis in recombination and mismatch correction."
      Wang T.-F., Kleckner N., Hunter N.
      Proc. Natl. Acad. Sci. U.S.A. 96:13914-13919(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH MLH1.
    6. "Discrete in vivo roles for the MutL homologs Mlh2p and Mlh3p in the removal of frameshift intermediates in budding yeast."
      Harfe B.D., Minesinger B.K., Jinks-Robertson S.
      Curr. Biol. 10:145-148(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    7. "Supercomplex formation between Mlh1-Mlh3 and Sgs1-Top3 heterocomplexes in meiotic yeast cells."
      Wang T.-F., Kung W.M.
      Biochem. Biophys. Res. Commun. 296:949-953(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH SGS1.

    Entry informationi

    Entry nameiMLH3_YEAST
    AccessioniPrimary (citable) accession number: Q12083
    Secondary accession number(s): D6W3K4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 11, 2006
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 99 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome XVI
      Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

    External Data

    Dasty 3