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Q12083 (MLH3_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 97. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
DNA mismatch repair protein MLH3
Alternative name(s):
MutL protein homolog 3
Gene names
Name:MLH3
Ordered Locus Names:YPL164C
ORF Names:P2550
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length715 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Involved in DNA mismatch repair (MMR), correcting insertion-deletion loops (IDLs) resulting from DNA replication, DNA damage or from recombination events between non-identical sequences during meiosis. Component of the MutLbeta heterodimer, which probably forms a ternary complex with the MutSbeta heterodimer that initially recognizes the DNA mismatches. This complex is thought to be responsible for directing the downsteam MMR events, including strand discrimination, excision, and resynthesis. Plays a major role in promoting meiotic crossing-over and is involved in maintaining the genetic stability of simple sequence repeats by correction of frameshift intermediates. Ref.4 Ref.5 Ref.6

Subunit structure

Heterodimer of MLH1 and MLH3, called MutLbeta, which is involved in correction of a specific subset of IDLs when associated with MutSbeta. Forms a ternary complex with a SGS1-TOP3 heterodimer during meiosis.

Subcellular location

Nucleus Probable.

Sequence similarities

Belongs to the DNA mismatch repair MutL/HexB family.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

MLH1P389204EBI-31634,EBI-11003

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 715715DNA mismatch repair protein MLH3
PRO_0000245570

Sequences

Sequence LengthMass (Da)Tools
Q12083 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 970FD7F57EB6E3B1

FASTA71582,001
        10         20         30         40         50         60 
MSQHIRKLDS DVSERLKSQA CTVSLASAVR EIVQNSVDAH ATTIDVMIDL PNLSFAVYDD 

        70         80         90        100        110        120 
GIGLTRSDLN ILATQNYTSK IRKMNDLVTM KTYGYRGDAL YSISNVSNLF VCSKKKDYNS 

       130        140        150        160        170        180 
AWMRKFPSKS VMLSENTILP IDPFWKICPW SRTKSGTVVI VEDMLYNLPV RRRILKEEPP 

       190        200        210        220        230        240 
FKTFNTIKAD MLQILVMHPM ISLNVQYTDK LRINTEVLFR SKNITEGLTK HQQMSQVLRN 

       250        260        270        280        290        300 
VFGAIIPPDM LKKVSLKFNE YQIEGIISKM PVGLKDLQFI YINGRRYADS AFQGYVDSLF 

       310        320        330        340        350        360 
QAQDFGEKGM SLLKTKSVGK PYRSHPVFIL DVRCPQTIDD LLQDPAKKIV KPSHIRTIEP 

       370        380        390        400        410        420 
LIVKTIRSFL TFQGYLTPDK SDSSFEIVNC SQKTATLPDS RIQISKRNQV LNSKMKIARI 

       430        440        450        460        470        480 
NSYIGKPAVN GCRINNSTIN YEKIKNIRID GQKSRLRNKL SSRPYDSGFT EDYDSIGKTI 

       490        500        510        520        530        540 
TDFSISRSVL AKYEVINQVD KKFILIRCLD QSIHNCPLLV LVDQHACDER IRLEELFYSL 

       550        560        570        580        590        600 
LTEVVTGTFV ARDLKDCCIE VDRTEADLFK HYQSEFKKWG IGYETIEGTM ETSLLEIKTL 

       610        620        630        640        650        660 
PEMLTSKYNG DKDYLKMVLL QHAHDLKDFK KLPMDLSHFE NYTSVDKLYW WKYSSCVPTV 

       670        680        690        700        710 
FHEILNSKAC RSAVMFGDEL TRQECIILIS KLSRCHNPFE CAHGRPSMVP IAELK 

« Hide

References

« Hide 'large scale' references
[1]"The sequence of 55 kb on the left arm of yeast chromosome XVI identifies a small nuclear RNA, a new putative protein kinase and two new putative regulators."
Purnelle B., Coster F., Goffeau A.
Yeast 12:1483-1492(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204511 / S288c / AB972.
[2]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI."
Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M. expand/collapse author list , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J.
Nature 387:103-105(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"The Saccharomyces cerevisiae MLH3 gene functions in MSH3-dependent suppression of frameshift mutations."
Flores-Rozas H., Kolodner R.D.
Proc. Natl. Acad. Sci. U.S.A. 95:12404-12409(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH MLH1.
[5]"Functional specificity of MutL homologs in yeast: evidence for three Mlh1-based heterocomplexes with distinct roles during meiosis in recombination and mismatch correction."
Wang T.-F., Kleckner N., Hunter N.
Proc. Natl. Acad. Sci. U.S.A. 96:13914-13919(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH MLH1.
[6]"Discrete in vivo roles for the MutL homologs Mlh2p and Mlh3p in the removal of frameshift intermediates in budding yeast."
Harfe B.D., Minesinger B.K., Jinks-Robertson S.
Curr. Biol. 10:145-148(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[7]"Supercomplex formation between Mlh1-Mlh3 and Sgs1-Top3 heterocomplexes in meiotic yeast cells."
Wang T.-F., Kung W.M.
Biochem. Biophys. Res. Commun. 296:949-953(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH SGS1.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X96770 Genomic DNA. Translation: CAA65557.1.
Z73520 Genomic DNA. Translation: CAA97869.1.
BK006949 Genomic DNA. Translation: DAA11270.1.
PIRS65175.
RefSeqNP_015161.1. NM_001183978.1.

3D structure databases

ProteinModelPortalQ12083.
SMRQ12083. Positions 26-289.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid36019. 10 interactions.
DIPDIP-2414N.
IntActQ12083. 3 interactions.
MINTMINT-678752.
STRING4932.YPL164C.

Proteomic databases

MaxQBQ12083.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYPL164C; YPL164C; YPL164C.
GeneID855939.
KEGGsce:YPL164C.

Organism-specific databases

CYGDYPL164c.
SGDS000006085. MLH3.

Phylogenomic databases

eggNOGCOG0323.
GeneTreeENSGT00550000074903.
HOGENOMHOG000113600.
KOK08739.
OMARIRVERY.
OrthoDBEOG77Q552.

Enzyme and pathway databases

BioCycYEAST:G3O-34060-MONOMER.

Gene expression databases

GenevestigatorQ12083.

Family and domain databases

Gene3D3.30.230.10. 1 hit.
3.30.565.10. 1 hit.
InterProIPR003594. HATPase_ATP-bd.
IPR028830. Mlh3.
IPR014790. MutL_C.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
[Graphical view]
PANTHERPTHR10073:SF7. PTHR10073:SF7. 1 hit.
PfamPF08676. MutL_C. 1 hit.
[Graphical view]
SMARTSM00853. MutL_C. 1 hit.
[Graphical view]
SUPFAMSSF54211. SSF54211. 1 hit.
SSF55874. SSF55874. 1 hit.
ProtoNetSearch...

Other

NextBio980696.
PROQ12083.

Entry information

Entry nameMLH3_YEAST
AccessionPrimary (citable) accession number: Q12083
Secondary accession number(s): D6W3K4
Entry history
Integrated into UniProtKB/Swiss-Prot: July 11, 2006
Last sequence update: November 1, 1996
Last modified: June 11, 2014
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XVI

Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families