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Protein

DNA mismatch repair protein MLH3

Gene

MLH3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Involved in DNA mismatch repair (MMR), correcting insertion-deletion loops (IDLs) resulting from DNA replication, DNA damage or from recombination events between non-identical sequences during meiosis. Component of the MutLbeta heterodimer, which probably forms a ternary complex with the MutSbeta heterodimer that initially recognizes the DNA mismatches. This complex is thought to be responsible for directing the downsteam MMR events, including strand discrimination, excision, and resynthesis. Plays a major role in promoting meiotic crossing-over and is involved in maintaining the genetic stability of simple sequence repeats by correction of frameshift intermediates.3 Publications

GO - Molecular functioni

  • ATPase activity Source: SGD
  • ATP binding Source: InterPro
  • mismatched DNA binding Source: InterPro
  • single-stranded DNA binding Source: GO_Central

GO - Biological processi

  • meiotic mismatch repair Source: SGD
  • mismatch repair Source: SGD
  • reciprocal meiotic recombination Source: SGD
Complete GO annotation...

Keywords - Biological processi

DNA damage, DNA repair

Enzyme and pathway databases

BioCyciYEAST:G3O-34060-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA mismatch repair protein MLH3
Alternative name(s):
MutL protein homolog 3
Gene namesi
Name:MLH3
Ordered Locus Names:YPL164C
ORF Names:P2550
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XVI

Organism-specific databases

EuPathDBiFungiDB:YPL164C.
SGDiS000006085. MLH3.

Subcellular locationi

GO - Cellular componenti

  • chiasma Source: GO_Central
  • MutLgamma complex Source: SGD
  • nucleus Source: SGD
  • synaptonemal complex Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 715715DNA mismatch repair protein MLH3PRO_0000245570Add
BLAST

Proteomic databases

MaxQBiQ12083.

Interactioni

Subunit structurei

Heterodimer of MLH1 and MLH3, called MutLbeta, which is involved in correction of a specific subset of IDLs when associated with MutSbeta. Forms a ternary complex with a SGS1-TOP3 heterodimer during meiosis.

Binary interactionsi

WithEntry#Exp.IntActNotes
MLH1P389204EBI-31634,EBI-11003

Protein-protein interaction databases

BioGridi36019. 13 interactions.
DIPiDIP-2414N.
IntActiQ12083. 3 interactions.
MINTiMINT-678752.

Structurei

3D structure databases

ProteinModelPortaliQ12083.
SMRiQ12083. Positions 26-289.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00800000124176.
HOGENOMiHOG000113600.
InParanoidiQ12083.
KOiK08739.
OMAiVDQHACD.
OrthoDBiEOG092C0TC4.

Family and domain databases

Gene3Di3.30.230.10. 1 hit.
3.30.565.10. 1 hit.
InterProiIPR013507. DNA_mismatch_repair_C.
IPR003594. HATPase_C.
IPR028830. Mlh3.
IPR014790. MutL_C.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
[Graphical view]
PANTHERiPTHR10073:SF7. PTHR10073:SF7. 2 hits.
SMARTiSM01340. DNA_mis_repair. 1 hit.
SM00853. MutL_C. 1 hit.
[Graphical view]
SUPFAMiSSF54211. SSF54211. 1 hit.
SSF55874. SSF55874. 1 hit.

Sequencei

Sequence statusi: Complete.

Q12083-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSQHIRKLDS DVSERLKSQA CTVSLASAVR EIVQNSVDAH ATTIDVMIDL
60 70 80 90 100
PNLSFAVYDD GIGLTRSDLN ILATQNYTSK IRKMNDLVTM KTYGYRGDAL
110 120 130 140 150
YSISNVSNLF VCSKKKDYNS AWMRKFPSKS VMLSENTILP IDPFWKICPW
160 170 180 190 200
SRTKSGTVVI VEDMLYNLPV RRRILKEEPP FKTFNTIKAD MLQILVMHPM
210 220 230 240 250
ISLNVQYTDK LRINTEVLFR SKNITEGLTK HQQMSQVLRN VFGAIIPPDM
260 270 280 290 300
LKKVSLKFNE YQIEGIISKM PVGLKDLQFI YINGRRYADS AFQGYVDSLF
310 320 330 340 350
QAQDFGEKGM SLLKTKSVGK PYRSHPVFIL DVRCPQTIDD LLQDPAKKIV
360 370 380 390 400
KPSHIRTIEP LIVKTIRSFL TFQGYLTPDK SDSSFEIVNC SQKTATLPDS
410 420 430 440 450
RIQISKRNQV LNSKMKIARI NSYIGKPAVN GCRINNSTIN YEKIKNIRID
460 470 480 490 500
GQKSRLRNKL SSRPYDSGFT EDYDSIGKTI TDFSISRSVL AKYEVINQVD
510 520 530 540 550
KKFILIRCLD QSIHNCPLLV LVDQHACDER IRLEELFYSL LTEVVTGTFV
560 570 580 590 600
ARDLKDCCIE VDRTEADLFK HYQSEFKKWG IGYETIEGTM ETSLLEIKTL
610 620 630 640 650
PEMLTSKYNG DKDYLKMVLL QHAHDLKDFK KLPMDLSHFE NYTSVDKLYW
660 670 680 690 700
WKYSSCVPTV FHEILNSKAC RSAVMFGDEL TRQECIILIS KLSRCHNPFE
710
CAHGRPSMVP IAELK
Length:715
Mass (Da):82,001
Last modified:November 1, 1996 - v1
Checksum:i970FD7F57EB6E3B1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X96770 Genomic DNA. Translation: CAA65557.1.
Z73520 Genomic DNA. Translation: CAA97869.1.
BK006949 Genomic DNA. Translation: DAA11270.1.
PIRiS65175.
RefSeqiNP_015161.1. NM_001183978.1.

Genome annotation databases

EnsemblFungiiYPL164C; YPL164C; YPL164C.
GeneIDi855939.
KEGGisce:YPL164C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X96770 Genomic DNA. Translation: CAA65557.1.
Z73520 Genomic DNA. Translation: CAA97869.1.
BK006949 Genomic DNA. Translation: DAA11270.1.
PIRiS65175.
RefSeqiNP_015161.1. NM_001183978.1.

3D structure databases

ProteinModelPortaliQ12083.
SMRiQ12083. Positions 26-289.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36019. 13 interactions.
DIPiDIP-2414N.
IntActiQ12083. 3 interactions.
MINTiMINT-678752.

Proteomic databases

MaxQBiQ12083.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYPL164C; YPL164C; YPL164C.
GeneIDi855939.
KEGGisce:YPL164C.

Organism-specific databases

EuPathDBiFungiDB:YPL164C.
SGDiS000006085. MLH3.

Phylogenomic databases

GeneTreeiENSGT00800000124176.
HOGENOMiHOG000113600.
InParanoidiQ12083.
KOiK08739.
OMAiVDQHACD.
OrthoDBiEOG092C0TC4.

Enzyme and pathway databases

BioCyciYEAST:G3O-34060-MONOMER.

Miscellaneous databases

PROiQ12083.

Family and domain databases

Gene3Di3.30.230.10. 1 hit.
3.30.565.10. 1 hit.
InterProiIPR013507. DNA_mismatch_repair_C.
IPR003594. HATPase_C.
IPR028830. Mlh3.
IPR014790. MutL_C.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
[Graphical view]
PANTHERiPTHR10073:SF7. PTHR10073:SF7. 2 hits.
SMARTiSM01340. DNA_mis_repair. 1 hit.
SM00853. MutL_C. 1 hit.
[Graphical view]
SUPFAMiSSF54211. SSF54211. 1 hit.
SSF55874. SSF55874. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiMLH3_YEAST
AccessioniPrimary (citable) accession number: Q12083
Secondary accession number(s): D6W3K4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 11, 2006
Last sequence update: November 1, 1996
Last modified: September 7, 2016
This is version 117 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XVI
    Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.