Q12083 (MLH3_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA mismatch repair protein MLH3

Alternative name(s):
MutL protein homolog 3

Gene names

Name:	MLH3
Ordered Locus Names:	YPL164C
ORF Names:	P2550

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	715 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in DNA mismatch repair (MMR), correcting insertion-deletion loops (IDLs) resulting from DNA replication, DNA damage or from recombination events between non-identical sequences during meiosis. Component of the MutLbeta heterodimer, which probably forms a ternary complex with the MutSbeta heterodimer that initially recognizes the DNA mismatches. This complex is thought to be responsible for directing the downsteam MMR events, including strand discrimination, excision, and resynthesis. Plays a major role in promoting meiotic crossing-over and is involved in maintaining the genetic stability of simple sequence repeats by corection of frameshift intermediates. Ref.4 Ref.5 Ref.6
Subunit structure	Heterodimer of MLH1 and MLH3, called MutLbeta, which is involved in correction of a specific subset of IDLs when associated with MutSbeta. Forms a ternary complex with a SGS1-TOP3 heterodimer during meiosis.
Subcellular location	Nucleus Probable.
Sequence similarities	Belongs to the DNA mismatch repair mutL/hexB family.

Ontologies

Keywords
Biological process	DNA damage DNA repair
Cellular component	Nucleus
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	mismatch repair Inferred from mutant phenotype Ref.6Ref.4. Source: SGD reciprocal meiotic recombination Inferred from mutant phenotype Ref.5. Source: SGD
Cellular component	MutLbeta complex Inferred from physical interaction Ref.4. Source: SGD chiasma Inferred from Biological aspect of Ancestor. Source: RefGenome synaptonemal complex Inferred from Biological aspect of Ancestor. Source: RefGenome
Molecular function	ATP binding Inferred from electronic annotation. Source: InterPro ATPase activity Inferred from Biological aspect of Ancestor. Source: RefGenome mismatched DNA binding Inferred from Biological aspect of Ancestor. Source: RefGenome protein binding Inferred from physical interaction Ref.5. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
MLH1	P38920	4	EBI-31634,EBI-11003

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 715	715	DNA mismatch repair protein MLH3		PRO_0000245570

Sequences

	Sequence		Length	Mass (Da)
	Q12083 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 970FD7F57EB6E3B1	FASTA	715	82,001
	10 20 30 40 50 60 MSQHIRKLDS DVSERLKSQA CTVSLASAVR EIVQNSVDAH ATTIDVMIDL PNLSFAVYDD 70 80 90 100 110 120 GIGLTRSDLN ILATQNYTSK IRKMNDLVTM KTYGYRGDAL YSISNVSNLF VCSKKKDYNS 130 140 150 160 170 180 AWMRKFPSKS VMLSENTILP IDPFWKICPW SRTKSGTVVI VEDMLYNLPV RRRILKEEPP 190 200 210 220 230 240 FKTFNTIKAD MLQILVMHPM ISLNVQYTDK LRINTEVLFR SKNITEGLTK HQQMSQVLRN 250 260 270 280 290 300 VFGAIIPPDM LKKVSLKFNE YQIEGIISKM PVGLKDLQFI YINGRRYADS AFQGYVDSLF 310 320 330 340 350 360 QAQDFGEKGM SLLKTKSVGK PYRSHPVFIL DVRCPQTIDD LLQDPAKKIV KPSHIRTIEP 370 380 390 400 410 420 LIVKTIRSFL TFQGYLTPDK SDSSFEIVNC SQKTATLPDS RIQISKRNQV LNSKMKIARI 430 440 450 460 470 480 NSYIGKPAVN GCRINNSTIN YEKIKNIRID GQKSRLRNKL SSRPYDSGFT EDYDSIGKTI 490 500 510 520 530 540 TDFSISRSVL AKYEVINQVD KKFILIRCLD QSIHNCPLLV LVDQHACDER IRLEELFYSL 550 560 570 580 590 600 LTEVVTGTFV ARDLKDCCIE VDRTEADLFK HYQSEFKKWG IGYETIEGTM ETSLLEIKTL 610 620 630 640 650 660 PEMLTSKYNG DKDYLKMVLL QHAHDLKDFK KLPMDLSHFE NYTSVDKLYW WKYSSCVPTV 670 680 690 700 710 FHEILNSKAC RSAVMFGDEL TRQECIILIS KLSRCHNPFE CAHGRPSMVP IAELK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The sequence of 55 kb on the left arm of yeast chromosome XVI identifies a small nuclear RNA, a new putative protein kinase and two new putative regulators." Purnelle B., Coster F., Goffeau A. Yeast 12:1483-1492(1996) [PubMed: 8948103] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI." Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M. , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J. Nature 387:103-105(1997) [PubMed: 9169875] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"The Saccharomyces cerevisiae MLH3 gene functions in MSH3-dependent suppression of frameshift mutations." Flores-Rozas H., Kolodner R.D. Proc. Natl. Acad. Sci. U.S.A. 95:12404-12409(1998) [PubMed: 9770499] [Abstract] Cited for: FUNCTION, INTERACTION WITH MLH1.
[5]	"Functional specificity of MutL homologs in yeast: evidence for three Mlh1-based heterocomplexes with distinct roles during meiosis in recombination and mismatch correction." Wang T.-F., Kleckner N., Hunter N. Proc. Natl. Acad. Sci. U.S.A. 96:13914-13919(1999) [PubMed: 10570173] [Abstract] Cited for: FUNCTION, INTERACTION WITH MLH1.
[6]	"Discrete in vivo roles for the MutL homologs Mlh2p and Mlh3p in the removal of frameshift intermediates in budding yeast." Harfe B.D., Minesinger B.K., Jinks-Robertson S. Curr. Biol. 10:145-148(2000) [PubMed: 10679328] [Abstract] Cited for: FUNCTION.
[7]	"Supercomplex formation between Mlh1-Mlh3 and Sgs1-Top3 heterocomplexes in meiotic yeast cells." Wang T.-F., Kung W.M. Biochem. Biophys. Res. Commun. 296:949-953(2002) [PubMed: 12200140] [Abstract] Cited for: INTERACTION WITH SGS1.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X96770 Genomic DNA. Translation: CAA65557.1. Z73520 Genomic DNA. Translation: CAA97869.1. BK006949 Genomic DNA. Translation: DAA11270.1.
PIR	S65175.
RefSeq	NP_015161.1. NM_001183978.1.
3D structure databases
ProteinModelPortal	Q12083.
SMR	Q12083. Positions 1-371.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-2414N.
IntAct	Q12083. 5 interactions.
MINT	MINT-678752.
STRING	Q12083.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YPL164C; YPL164C; YPL164C.
GeneID	855939.
KEGG	sce:YPL164C.
NMPDR	fig|4932.3.peg.6290.
Organism-specific databases
CYGD	YPL164c.
SGD	S000006085. MLH3.
Phylogenomic databases
eggNOG	fuNOG05561.
GeneTree	EFGT00050000002853.
HOGENOM	HBG203869.
OMA	KACRSAI.
OrthoDB	EOG46X2HJ.
Gene expression databases
ArrayExpress	Q12083.
Genevestigator	Q12083.
GermOnline	YPL164C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR003594. ATPase-like_ATP-bd. IPR002099. DNA_mismatch_repair. IPR014790. MutL_C. IPR020568. Ribosomal_S5_D2-typ_fold. IPR014721. Ribosomal_S5_D2-typ_fold_subgr. [Graphical view]
Gene3D	G3DSA:3.30.565.10. ATP_bd_ATPase. 1 hit. G3DSA:3.30.230.10. Ribosomal_S5_D2-type_fold. 1 hit.
KO	K08739.
PANTHER	PTHR10073. DNA_mis_repair. 1 hit.
Pfam	PF02518. HATPase_c. 1 hit. PF08676. MutL_C. 1 hit. [Graphical view]
SMART	SM00853. MutL_C. 1 hit. [Graphical view]
SUPFAM	SSF55874. ATP_bd_ATPase. 1 hit. SSF54211. Ribosomal_S5_D2-typ_fold. 1 hit.
ProtoNet	Search...
Other
NextBio	980696.

Entry information

Entry name

MLH3_YEAST

Accession

Primary (citable) accession number: Q12083
Secondary accession number(s): D6W3K4

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 11, 2006
Last sequence update:	November 1, 1996
Last modified:	January 25, 2012
This is version 74 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program