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Protein

Spermidine synthase

Gene

SPE3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalytic activityi

S-adenosyl 3-(methylthio)propylamine + putrescine = 5'-S-methyl-5'-thioadenosine + spermidine.

Pathwayi: spermidine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes spermidine from putrescine.
Proteins known to be involved in this subpathway in this organism are:
  1. Spermidine synthase (SPE3)
This subpathway is part of the pathway spermidine biosynthesis, which is itself part of Amine and polyamine biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes spermidine from putrescine, the pathway spermidine biosynthesis and in Amine and polyamine biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei44S-adenosylmethioninamineBy similarity1
Binding sitei74PutrescineBy similarity1
Binding sitei75S-adenosylmethioninamineBy similarity1
Binding sitei99S-adenosylmethioninamineBy similarity1
Binding sitei119S-adenosylmethioninamineBy similarity1
Active sitei168Proton acceptorBy similarity1
Binding sitei168S-adenosylmethioninamineBy similarity1
Binding sitei236PutrescineBy similarity1

GO - Molecular functioni

  • spermidine synthase activity Source: SGD

GO - Biological processi

  • pantothenate biosynthetic process Source: SGD
  • spermidine biosynthetic process Source: SGD

Keywordsi

Molecular functionTransferase
Biological processPolyamine biosynthesis, Spermidine biosynthesis

Enzyme and pathway databases

BioCyciMetaCyc:YPR069C-MONOMER
YEAST:YPR069C-MONOMER
ReactomeiR-SCE-351202 Metabolism of polyamines
UniPathwayiUPA00248; UER00314

Names & Taxonomyi

Protein namesi
Recommended name:
Spermidine synthase (EC:2.5.1.16)
Short name:
SPDSY
Alternative name(s):
Putrescine aminopropyltransferase
Gene namesi
Name:SPE3
Ordered Locus Names:YPR069C
ORF Names:YP9499.24C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XVI

Organism-specific databases

EuPathDBiFungiDB:YPR069C
SGDiS000006273 SPE3

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001564621 – 293Spermidine synthaseAdd BLAST293

Proteomic databases

MaxQBiQ12074
PaxDbiQ12074
PRIDEiQ12074

Interactioni

Protein-protein interaction databases

BioGridi36241, 314 interactors
DIPiDIP-6618N
IntActiQ12074, 5 interactors
MINTiQ12074
STRINGi4932.YPR069C

Structurei

3D structure databases

ProteinModelPortaliQ12074
SMRiQ12074
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini12 – 248PABSAdd BLAST237

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni150 – 151S-adenosylmethioninamine bindingBy similarity2
Regioni168 – 171Putrescine bindingBy similarity4

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00870000136521
HOGENOMiHOG000256147
InParanoidiQ12074
KOiK00797
OMAiLWPGQSF
OrthoDBiEOG092C2J3O

Family and domain databases

Gene3Di2.30.140.10, 1 hit
HAMAPiMF_00198 Spermidine_synth, 1 hit
InterProiView protein in InterPro
IPR030374 PABS
IPR030373 PABS_CS
IPR029063 SAM-dependent_MTases
IPR001045 Spermi_synthase
IPR030668 Spermi_synthase_euk
IPR035246 Spermidine_synt_N
IPR037163 Spermidine_synt_N_sf
PfamiView protein in Pfam
PF17284 Spermine_synt_N, 1 hit
PIRSFiPIRSF000502 Spermidine_synth, 1 hit
SUPFAMiSSF53335 SSF53335, 1 hit
TIGRFAMsiTIGR00417 speE, 1 hit
PROSITEiView protein in PROSITE
PS01330 PABS_1, 1 hit
PS51006 PABS_2, 1 hit

Sequencei

Sequence statusi: Complete.

Q12074-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAQEITHPTI VDGWFREISD TMWPGQAMTL KVEKVLHHEK SKYQDVLIFK
60 70 80 90 100
STTYGNVLVL DNVIQATERD EFAYQEMIAH LALNSHPNPK KVLVIGGGDG
110 120 130 140 150
GVLREVVKHD SVEEAWLCDI DEAVIRLSKE YLPEMAASYS HPKVKTHIGD
160 170 180 190 200
GFQFLRDYQN TFDVIITDSS DPEGPAETLF QKEYFQLLNS ALTEKGVITT
210 220 230 240 250
QAESMWIHLP IIKDLKKACS EVFPVAEYSF VTIPTYPTGT IGFMVCSKDK
260 270 280 290
TCNVKKPLRE ISDEKEAELY RYYNKKIHEA SFVLPTWAAK ELN
Length:293
Mass (Da):33,324
Last modified:November 1, 1996 - v1
Checksum:i5B1B361E084ED09F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U27519 Genomic DNA Translation: AAC17191.1
Z71255 Genomic DNA Translation: CAA94977.1
Z49219 Genomic DNA Translation: CAA89186.1
U51033 Genomic DNA Translation: AAB68120.1
BK006949 Genomic DNA Translation: DAA11489.1
PIRiS54090
RefSeqiNP_015394.1, NM_001184166.1

Genome annotation databases

EnsemblFungiiYPR069C; YPR069C; YPR069C
GeneIDi856182
KEGGisce:YPR069C

Similar proteinsi

Entry informationi

Entry nameiSPEE_YEAST
AccessioniPrimary (citable) accession number: Q12074
Secondary accession number(s): D6W473
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: May 23, 2018
This is version 148 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health