Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Spermidine synthase

Gene

SPE3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

S-adenosyl 3-(methylthio)propylamine + putrescine = 5'-S-methyl-5'-thioadenosine + spermidine.

Pathwayi: spermidine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes spermidine from putrescine.
Proteins known to be involved in this subpathway in this organism are:
  1. Spermidine synthase (SPE3)
This subpathway is part of the pathway spermidine biosynthesis, which is itself part of Amine and polyamine biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes spermidine from putrescine, the pathway spermidine biosynthesis and in Amine and polyamine biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei44 – 441S-adenosylmethioninamineBy similarity
Binding sitei74 – 741PutrescineBy similarity
Binding sitei75 – 751S-adenosylmethioninamineBy similarity
Binding sitei99 – 991S-adenosylmethioninamineBy similarity
Binding sitei119 – 1191S-adenosylmethioninamineBy similarity
Active sitei168 – 1681Proton acceptorBy similarity
Binding sitei168 – 1681S-adenosylmethioninamineBy similarity
Binding sitei236 – 2361PutrescineBy similarity

GO - Molecular functioni

  • spermidine synthase activity Source: SGD

GO - Biological processi

  • pantothenate biosynthetic process Source: SGD
  • spermidine biosynthetic process Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Polyamine biosynthesis, Spermidine biosynthesis

Enzyme and pathway databases

BioCyciMetaCyc:YPR069C-MONOMER.
YEAST:YPR069C-MONOMER.
ReactomeiR-SCE-351202. Metabolism of polyamines.
UniPathwayiUPA00248; UER00314.

Names & Taxonomyi

Protein namesi
Recommended name:
Spermidine synthase (EC:2.5.1.16)
Short name:
SPDSY
Alternative name(s):
Putrescine aminopropyltransferase
Gene namesi
Name:SPE3
Ordered Locus Names:YPR069C
ORF Names:YP9499.24C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XVI

Organism-specific databases

EuPathDBiFungiDB:YPR069C.
SGDiS000006273. SPE3.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 293293Spermidine synthasePRO_0000156462Add
BLAST

Proteomic databases

MaxQBiQ12074.
PeptideAtlasiQ12074.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
SPE4Q124553EBI-17836,EBI-17907

Protein-protein interaction databases

BioGridi36241. 98 interactions.
DIPiDIP-6618N.
IntActiQ12074. 3 interactions.
MINTiMINT-644204.

Structurei

3D structure databases

ProteinModelPortaliQ12074.
SMRiQ12074. Positions 10-293.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini12 – 248237PABSAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni150 – 1512S-adenosylmethioninamine bindingBy similarity
Regioni168 – 1714Putrescine bindingBy similarity

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00620000088039.
HOGENOMiHOG000256147.
InParanoidiQ12074.
KOiK00797.
OMAiLEAYCTT.
OrthoDBiEOG7FJH8S.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_00198. Spermidine_synth.
InterProiIPR030374. PABS.
IPR030373. PABS_CS.
IPR029063. SAM-dependent_MTases.
IPR001045. Spermi_synthase.
IPR030668. Spermi_synthase_euk.
[Graphical view]
PIRSFiPIRSF000502. Spermidine_synth. 1 hit.
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00417. speE. 1 hit.
PROSITEiPS01330. PABS_1. 1 hit.
PS51006. PABS_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q12074-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAQEITHPTI VDGWFREISD TMWPGQAMTL KVEKVLHHEK SKYQDVLIFK
60 70 80 90 100
STTYGNVLVL DNVIQATERD EFAYQEMIAH LALNSHPNPK KVLVIGGGDG
110 120 130 140 150
GVLREVVKHD SVEEAWLCDI DEAVIRLSKE YLPEMAASYS HPKVKTHIGD
160 170 180 190 200
GFQFLRDYQN TFDVIITDSS DPEGPAETLF QKEYFQLLNS ALTEKGVITT
210 220 230 240 250
QAESMWIHLP IIKDLKKACS EVFPVAEYSF VTIPTYPTGT IGFMVCSKDK
260 270 280 290
TCNVKKPLRE ISDEKEAELY RYYNKKIHEA SFVLPTWAAK ELN
Length:293
Mass (Da):33,324
Last modified:November 1, 1996 - v1
Checksum:i5B1B361E084ED09F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U27519 Genomic DNA. Translation: AAC17191.1.
Z71255 Genomic DNA. Translation: CAA94977.1.
Z49219 Genomic DNA. Translation: CAA89186.1.
U51033 Genomic DNA. Translation: AAB68120.1.
BK006949 Genomic DNA. Translation: DAA11489.1.
PIRiS54090.
RefSeqiNP_015394.1. NM_001184166.1.

Genome annotation databases

EnsemblFungiiYPR069C; YPR069C; YPR069C.
GeneIDi856182.
KEGGisce:YPR069C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U27519 Genomic DNA. Translation: AAC17191.1.
Z71255 Genomic DNA. Translation: CAA94977.1.
Z49219 Genomic DNA. Translation: CAA89186.1.
U51033 Genomic DNA. Translation: AAB68120.1.
BK006949 Genomic DNA. Translation: DAA11489.1.
PIRiS54090.
RefSeqiNP_015394.1. NM_001184166.1.

3D structure databases

ProteinModelPortaliQ12074.
SMRiQ12074. Positions 10-293.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36241. 98 interactions.
DIPiDIP-6618N.
IntActiQ12074. 3 interactions.
MINTiMINT-644204.

Proteomic databases

MaxQBiQ12074.
PeptideAtlasiQ12074.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYPR069C; YPR069C; YPR069C.
GeneIDi856182.
KEGGisce:YPR069C.

Organism-specific databases

EuPathDBiFungiDB:YPR069C.
SGDiS000006273. SPE3.

Phylogenomic databases

GeneTreeiENSGT00620000088039.
HOGENOMiHOG000256147.
InParanoidiQ12074.
KOiK00797.
OMAiLEAYCTT.
OrthoDBiEOG7FJH8S.

Enzyme and pathway databases

UniPathwayiUPA00248; UER00314.
BioCyciMetaCyc:YPR069C-MONOMER.
YEAST:YPR069C-MONOMER.
ReactomeiR-SCE-351202. Metabolism of polyamines.

Miscellaneous databases

PROiQ12074.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_00198. Spermidine_synth.
InterProiIPR030374. PABS.
IPR030373. PABS_CS.
IPR029063. SAM-dependent_MTases.
IPR001045. Spermi_synthase.
IPR030668. Spermi_synthase_euk.
[Graphical view]
PIRSFiPIRSF000502. Spermidine_synth. 1 hit.
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00417. speE. 1 hit.
PROSITEiPS01330. PABS_1. 1 hit.
PS51006. PABS_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Spermidine biosynthesis in Saccharomyces cerevisae: polyamine requirement of a null mutant of the SPE3 gene (spermidine synthase)."
    Hamasaki-Katagiri N., Tabor C.W., Tabor H.
    Gene 187:35-43(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI."
    Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M.
    , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J.
    Nature 387:103-105(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.

Entry informationi

Entry nameiSPEE_YEAST
AccessioniPrimary (citable) accession number: Q12074
Secondary accession number(s): D6W473
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: June 8, 2016
This is version 129 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XVI
    Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.