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Protein

NADPH-dependent methylglyoxal reductase GRE2

Gene

GRE2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the irreversible reduction of the cytotoxic compound methylglyoxal (MG, 2-oxopropanal) to (S)-lactaldehyde as an alternative to detoxification of MG by glyoxalase I GLO1. MG is synthesized via a bypath of glycolysis from dihydroxyacetone phosphate and is believed to play a role in cell cycle regulation and stress adaptation. Also catalyzes the reduction of 3-methylbutanal to 3-methylbutanol. Acts as a suppressor of 3-methylbutanol-induced filamentation by modulating the levels of 3-methylbutanal, the signal to which cells respond by filamentation. Also involved in ergosterol metabolism.4 Publications

Catalytic activityi

(S)-lactaldehyde + NADP+ = 2-oxopropanal + NADPH.3 Publications
3-methylbutanol + NAD(P)+ = 3-methylbutanal + NAD(P)H.1 Publication

Enzyme regulationi

Activated by glutathione.

Kineticsi

kcat is 31.3 min(-1) with 3-chloro-1-phenyl-1-propanol as substrate and 29.1 min(-1) for NADPH.

  1. KM=5.88 mM for methylglyoxal1 Publication
  2. KM=1.54 mM for phenylglyoxal1 Publication
  3. KM=0.14 mM for 3-methylbutanal1 Publication
  4. KM=0.192 mM for 3-chloro-1-phenyl-1-propanol2 Publications
  5. KM=4.33 mM for 2,5-hexanedione2 Publications
  6. KM=10.48 mM for (2S,5S)-hexanediol2 Publications
  7. KM=0.112 mM for NADPH2 Publications

    pH dependencei

    Optimum pH is 7 for 2,5-hexanedione reduction, and 10 for the reverse reaction.2 Publications

    Temperature dependencei

    Optimum temperature is 40 degrees Celsius for 3-chloro-1-phenyl-1-propanol reduction, and 54 degrees Celsius for 2,5-hexanedione reduction, and 30 degrees Celsius for the reverse reaction.2 Publications

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei32 – 321NADP1 Publication
    Binding sitei36 – 361NADP1 Publication
    Binding sitei165 – 1651NADP1 Publication
    Active sitei169 – 1691Proton donor1 Publication
    Binding sitei169 – 1691NADP1 Publication
    Binding sitei199 – 1991NADP; via amide nitrogen1 Publication
    Binding sitei216 – 2161NADP1 Publication

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi7 – 126NADP1 Publication
    Nucleotide bindingi57 – 582NADP1 Publication

    GO - Molecular functioni

    • 3-beta-hydroxy-delta5-steroid dehydrogenase activity Source: InterPro
    • 3-methylbutanol:NAD(P) oxidoreductase activity Source: SGD
    • methylglyoxal reductase (NADPH-dependent) activity Source: SGD

    GO - Biological processi

    • ergosterol metabolic process Source: SGD
    • filamentous growth Source: SGD
    • steroid biosynthetic process Source: InterPro
    Complete GO annotation...

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Ligandi

    NADP

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-12909.
    YEAST:YOL151W-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    NADPH-dependent methylglyoxal reductase GRE2 (EC:1.1.1.2833 Publications)
    Alternative name(s):
    3-methylbutanal reductase (EC:1.1.1.2651 Publication)
    Genes de respuesta a estres protein 2
    Isovaleraldehyde reductase
    Gene namesi
    Name:GRE2
    Ordered Locus Names:YOL151W
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    Proteomesi
    • UP000002311 Componenti: Chromosome XV

    Organism-specific databases

    EuPathDBiFungiDB:YOL151W.
    SGDiS000005511. GRE2.

    Subcellular locationi

    GO - Cellular componenti

    • cytoplasm Source: SGD
    • nucleus Source: SGD
    Complete GO annotation...

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    Pathology & Biotechi

    Biotechnological usei

    Used as a biocatalyst, because the enzyme accepts a broad range of substrates including aliphatic and aromatic ketones, chloroketones, diketones as well as beta-ketoesters which are reduced with high stereoselectivity.2 Publications

    Disruption phenotypei

    Causes hyperfilamentation, probably due to the elevated levels of 3-methylbutanal in the mutant.1 Publication

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi9 – 91N → E: Alters cofactor preference of the enzyme to be able to use as well NAD instead of NADP. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 342342NADPH-dependent methylglyoxal reductase GRE2PRO_0000215576Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei333 – 3331PhosphoserineCombined sources

    Post-translational modificationi

    The N-terminus is blocked.

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiQ12068.

    PTM databases

    iPTMnetiQ12068.

    Expressioni

    Inductioni

    Repressed by SKO1. During osmotic stress, this repression is relieved. Induced by transcription factor YAP1 during oxidative stress, and induced by ionic and heat stress. Induced by isoamylalcohol.4 Publications

    Interactioni

    Subunit structurei

    Monomer.1 Publication

    Protein-protein interaction databases

    BioGridi34267. 25 interactions.
    DIPiDIP-2645N.
    IntActiQ12068. 1 interaction.
    MINTiMINT-425047.

    Structurei

    Secondary structure

    1
    342
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi3 – 64Combined sources
    Turni7 – 93Combined sources
    Helixi11 – 2212Combined sources
    Beta strandi27 – 337Combined sources
    Helixi34 – 4310Combined sources
    Beta strandi50 – 545Combined sources
    Turni61 – 644Combined sources
    Helixi65 – 717Combined sources
    Turni72 – 743Combined sources
    Beta strandi77 – 804Combined sources
    Beta strandi89 – 913Combined sources
    Helixi92 – 954Combined sources
    Helixi97 – 11418Combined sources
    Turni116 – 1183Combined sources
    Beta strandi121 – 1255Combined sources
    Helixi128 – 1303Combined sources
    Helixi134 – 1363Combined sources
    Beta strandi143 – 1453Combined sources
    Helixi156 – 1594Combined sources
    Helixi161 – 18222Combined sources
    Turni183 – 1864Combined sources
    Beta strandi190 – 1967Combined sources
    Beta strandi198 – 2014Combined sources
    Helixi206 – 2105Combined sources
    Helixi215 – 22410Combined sources
    Beta strandi236 – 2416Combined sources
    Helixi242 – 25413Combined sources
    Turni257 – 2604Combined sources
    Beta strandi262 – 2654Combined sources
    Beta strandi268 – 2714Combined sources
    Helixi272 – 28211Combined sources
    Turni284 – 2896Combined sources
    Turni299 – 3013Combined sources
    Beta strandi307 – 3093Combined sources
    Helixi311 – 3177Combined sources
    Helixi324 – 33815Combined sources

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    4PVCX-ray2.00A/B1-342[»]
    4PVDX-ray2.40A/B/C/D1-342[»]
    ProteinModelPortaliQ12068.
    SMRiQ12068. Positions 1-342.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    GeneTreeiENSGT00390000002618.
    HOGENOMiHOG000167998.
    InParanoidiQ12068.
    KOiK17741.
    OMAiFINDETT.
    OrthoDBiEOG092C3RD2.

    Family and domain databases

    Gene3Di3.40.50.720. 1 hit.
    InterProiIPR002225. 3Beta_OHSteriod_DH/Estase.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view]
    PfamiPF01073. 3Beta_HSD. 1 hit.
    [Graphical view]
    SUPFAMiSSF51735. SSF51735. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q12068-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MSVFVSGANG FIAQHIVDLL LKEDYKVIGS ARSQEKAENL TEAFGNNPKF
    60 70 80 90 100
    SMEVVPDISK LDAFDHVFQK HGKDIKIVLH TASPFCFDIT DSERDLLIPA
    110 120 130 140 150
    VNGVKGILHS IKKYAADSVE RVVLTSSYAA VFDMAKENDK SLTFNEESWN
    160 170 180 190 200
    PATWESCQSD PVNAYCGSKK FAEKAAWEFL EENRDSVKFE LTAVNPVYVF
    210 220 230 240 250
    GPQMFDKDVK KHLNTSCELV NSLMHLSPED KIPELFGGYI DVRDVAKAHL
    260 270 280 290 300
    VAFQKRETIG QRLIVSEARF TMQDVLDILN EDFPVLKGNI PVGKPGSGAT
    310 320 330 340
    HNTLGATLDN KKSKKLLGFK FRNLKETIDD TASQILKFEG RI
    Length:342
    Mass (Da):38,170
    Last modified:November 1, 1996 - v1
    Checksum:i84DC286AAD8C88D2
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    Z48239 Genomic DNA. Translation: CAA88277.1.
    Z74893 Genomic DNA. Translation: CAA99172.1.
    AY558040 Genomic DNA. Translation: AAS56366.1.
    BK006948 Genomic DNA. Translation: DAA10635.1.
    PIRiS60386.
    RefSeqiNP_014490.1. NM_001183405.1.

    Genome annotation databases

    EnsemblFungiiYOL151W; YOL151W; YOL151W.
    GeneIDi854014.
    KEGGisce:YOL151W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    Z48239 Genomic DNA. Translation: CAA88277.1.
    Z74893 Genomic DNA. Translation: CAA99172.1.
    AY558040 Genomic DNA. Translation: AAS56366.1.
    BK006948 Genomic DNA. Translation: DAA10635.1.
    PIRiS60386.
    RefSeqiNP_014490.1. NM_001183405.1.

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    4PVCX-ray2.00A/B1-342[»]
    4PVDX-ray2.40A/B/C/D1-342[»]
    ProteinModelPortaliQ12068.
    SMRiQ12068. Positions 1-342.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    BioGridi34267. 25 interactions.
    DIPiDIP-2645N.
    IntActiQ12068. 1 interaction.
    MINTiMINT-425047.

    PTM databases

    iPTMnetiQ12068.

    Proteomic databases

    MaxQBiQ12068.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblFungiiYOL151W; YOL151W; YOL151W.
    GeneIDi854014.
    KEGGisce:YOL151W.

    Organism-specific databases

    EuPathDBiFungiDB:YOL151W.
    SGDiS000005511. GRE2.

    Phylogenomic databases

    GeneTreeiENSGT00390000002618.
    HOGENOMiHOG000167998.
    InParanoidiQ12068.
    KOiK17741.
    OMAiFINDETT.
    OrthoDBiEOG092C3RD2.

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-12909.
    YEAST:YOL151W-MONOMER.

    Miscellaneous databases

    PROiQ12068.

    Family and domain databases

    Gene3Di3.40.50.720. 1 hit.
    InterProiIPR002225. 3Beta_OHSteriod_DH/Estase.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view]
    PfamiPF01073. 3Beta_HSD. 1 hit.
    [Graphical view]
    SUPFAMiSSF51735. SSF51735. 1 hit.
    ProtoNetiSearch...

    Entry informationi

    Entry nameiGRE2_YEAST
    AccessioniPrimary (citable) accession number: Q12068
    Secondary accession number(s): D6W1R9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 25, 2004
    Last sequence update: November 1, 1996
    Last modified: September 7, 2016
    This is version 124 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 5458 molecules/cell in log phase SD medium.1 Publication
    'De respuesta a estres' means stress response in Spanish.

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome XV
      Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.