Q12068 (GRE2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
NADPH-dependent methylglyoxal reductase GRE2
EC=1.1.1.283

Alternative name(s):
Genes de respuesta a estres protein 2

Gene names

Name:	GRE2
Ordered Locus Names:	YOL151W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	342 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes the irreversible reduction of the cytotoxic compound methylglyoxal (MG) to (R)-lactaldehyde as an alternative to detoxification of MG by glyoxalase I GLO1. MG is synthesized via a bypath of glycolysis from dihydroxyacetone phosphate and is believed to play a role in cell cycle regulation and stress adaptation. Ref.5 Ref.6
Catalytic activity	Lactaldehyde + NADP+ = methylglyoxal + NADPH. Ref.5 Ref.6
Enzyme regulation	Activated by glutathione.
Subcellular location	Cytoplasm. Nucleus Ref.10.
Induction	Repressed by SKO1. During osmotic stress, this repression is relieved. Induced by transcription factor YAP1 during oxidative stress, and induced by ionic and heat stress. Ref.7 Ref.8 Ref.9
Miscellaneous	Present with 5458 molecules/cell in log phase SD medium. Ref.11 'De respuesta a estres' means stress response in Spanish.
Sequence similarities	Belongs to the dihydroflavonol-4-reductase family.

Ontologies

Keywords
Cellular component	Cytoplasm Nucleus
Ligand	NADP
Molecular function	Oxidoreductase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	ergosterol metabolic process Inferred from mutant phenotype. Source: SGD filamentous growth Inferred from mutant phenotype. Source: SGD
Cellular component	cytoplasm Inferred from direct assay. Source: SGD nucleus Inferred from direct assay. Source: SGD
Molecular function	3-methylbutanol:NAD(P) oxidoreductase activity Inferred from mutant phenotype. Source: SGD coenzyme binding Inferred from electronic annotation. Source: InterPro methylglyoxal reductase (NADPH-dependent) activity Inferred from direct assay Ref.6. Source: SGD nucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 342	342	NADPH-dependent methylglyoxal reductase GRE2		PRO_0000215576
Amino acid modifications
	Modified residue	333	1	Phosphoserine Ref.12

Sequences

	Sequence		Length	Mass (Da)
	Q12068 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 84DC286AAD8C88D2	FASTA	342	38,170
	10 20 30 40 50 60 MSVFVSGANG FIAQHIVDLL LKEDYKVIGS ARSQEKAENL TEAFGNNPKF SMEVVPDISK 70 80 90 100 110 120 LDAFDHVFQK HGKDIKIVLH TASPFCFDIT DSERDLLIPA VNGVKGILHS IKKYAADSVE 130 140 150 160 170 180 RVVLTSSYAA VFDMAKENDK SLTFNEESWN PATWESCQSD PVNAYCGSKK FAEKAAWEFL 190 200 210 220 230 240 EENRDSVKFE LTAVNPVYVF GPQMFDKDVK KHLNTSCELV NSLMHLSPED KIPELFGGYI 250 260 270 280 290 300 DVRDVAKAHL VAFQKRETIG QRLIVSEARF TMQDVLDILN EDFPVLKGNI PVGKPGSGAT 310 320 330 340 HNTLGATLDN KKSKKLLGFK FRNLKETIDD TASQILKFEG RI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"DNA sequence analysis of a 13 kbp fragment of the left arm of yeast chromosome XV containing seven new open reading frames." Casamayor A., Aldea M., Casas C., Herrero E., Gamo F.-J., Lafuente M.J., Gancedo C., Arino J. Yeast 11:1281-1288(1995) [PubMed: 8553699] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XV." Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D. , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K. Nature 387:98-102(1997) [PubMed: 9169874] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[5]	"Metabolism of 2-oxoaldehyde in yeasts. Purification and characterization of NADPH-dependent methylglyoxal-reducing enzyme from Saccharomyces cerevisiae." Murata K., Fukuda Y., Simosaka M., Watanabe K., Saikusa T., Kimura A. Eur. J. Biochem. 151:631-636(1985) [PubMed: 3896793] [Abstract] Cited for: FUNCTION, CATALYTIC ACTIVITY.
[6]	"Associating protein activities with their genes: rapid identification of a gene encoding a methylglyoxal reductase in the yeast Saccharomyces cerevisiae." Chen C.N., Porubleva L., Shearer G., Svrakic M., Holden L.G., Dover J.L., Johnston M., Chitnis P.R., Kohl D.H. Yeast 20:545-554(2003) [PubMed: 12722185] [Abstract] Cited for: FUNCTION, CATALYTIC ACTIVITY.
[7]	"Three genes whose expression is induced by stress in Saccharomyces cerevisiae." Garay-Arroyo A., Covarrubias A.A. Yeast 15:879-892(1999) [PubMed: 10407268] [Abstract] Cited for: INDUCTION.
[8]	"Yap1 and Skn7 control two specialized oxidative stress response regulons in yeast." Lee J., Godon C., Lagniel G., Spector D., Garin J., Labarre J., Toledano M.B. J. Biol. Chem. 274:16040-16046(1999) [PubMed: 10347154] [Abstract] Cited for: INDUCTION.
[9]	"The Saccharomyces cerevisiae Sko1p transcription factor mediates HOG pathway-dependent osmotic regulation of a set of genes encoding enzymes implicated in protection from oxidative damage." Rep M., Proft M., Remize F., Tamas M., Serrano R., Thevelein J.M., Hohmann S. Mol. Microbiol. 40:1067-1083(2001) [PubMed: 11401713] [Abstract] Cited for: INDUCTION.
[10]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[11]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[12]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-333, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z48239 Genomic DNA. Translation: CAA88277.1. Z74893 Genomic DNA. Translation: CAA99172.1. AY558040 Genomic DNA. Translation: AAS56366.1. BK006948 Genomic DNA. Translation: DAA10635.1.
PIR	S60386.
RefSeq	NP_014490.1. NM_001183405.1.
3D structure databases
ProteinModelPortal	Q12068.
SMR	Q12068. Positions 2-342.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-2645N.
MINT	MINT-425047.
STRING	Q12068.
Proteomic databases
PeptideAtlas	Q12068.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YOL151W; YOL151W; YOL151W.
GeneID	854014.
KEGG	sce:YOL151W.
NMPDR	fig|4932.3.peg.5576.
Organism-specific databases
CYGD	YOL151w.
SGD	S000005511. GRE2.
Phylogenomic databases
eggNOG	fuNOG07932.
GeneTree	EFGT00050000001225.
HOGENOM	HBG755066.
OMA	TAAVHYN.
OrthoDB	EOG480N5D.
Enzyme and pathway databases
BioCyc	MetaCyc:MONOMER-12909.
Gene expression databases
ArrayExpress	Q12068.
Genevestigator	Q12068.
GermOnline	YOL151W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR001509. Epimerase_deHydtase. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF01370. Epimerase. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	975535.

Entry information

Entry name

GRE2_YEAST

Accession

Primary (citable) accession number: Q12068
Secondary accession number(s): D6W1R9

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 25, 2004
Last sequence update:	November 1, 1996
Last modified:	December 14, 2011
This is version 87 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program